Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P74936 (PROB_TREPA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate 5-kinase

EC=2.7.2.11
Alternative name(s):
Gamma-glutamyl kinase
Short name=GK
Gene names
Name:proB
Ordered Locus Names:TP_0351
OrganismTreponema pallidum (strain Nichols) [Complete proteome] [HAMAP]
Taxonomic identifier243276 [NCBI]
Taxonomic lineageBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeTreponema

Protein attributes

Sequence length296 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP-Rule MF_00456

Catalytic activity

ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP-Rule MF_00456

Pathway

Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP-Rule MF_00456

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00456.

Sequence similarities

Belongs to the glutamate 5-kinase family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Proline biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processL-proline biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate 5-kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 296296Glutamate 5-kinase HAMAP-Rule MF_00456
PRO_0000109750

Regions

Nucleotide binding206 – 2072ATP By similarity
Nucleotide binding248 – 2547ATP By similarity

Sites

Binding site151ATP By similarity
Binding site551Substrate By similarity
Binding site1591Substrate By similarity
Binding site1861Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
P74936 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 4276927F287E4F14

FASTA29631,315
        10         20         30         40         50         60 
MIRALFAAAK KIVIKIGSNT LAQADGTPDE EFLAECARAC AALMRDGKQI VVVSSGAQVA 

        70         80         90        100        110        120 
GISALHCLSS PPQGAGLERH ESRGVIPGDG ASCKQALCAV GQAELISRWR SAFAAHQQCV 

       130        140        150        160        170        180 
GQFLCTKEDF TDSDRAAQVR YTLSFLLERR VVPILNENDA LCCSDVPSVP ADRRVSLSPQ 

       190        200        210        220        230        240 
KRIGDNDSLS AFVALLWQAD LLLLLSDIDG VYDKDPKAHT DAQHVPLVTD VSALVGKTSM 

       250        260        270        280        290 
GSSNVFGTGG IATKLDAARL VTRAGIPLVL ANGRHLDPIL SLMRGDARGT LFVPVS 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequences of the proA and proB genes of Treponema pallidum, the syphilis agent."
Stamm L.V., Barnes N.Y.
DNA Seq. 8:63-70(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Nichols.
[2]"Complete genome sequence of Treponema pallidum, the syphilis spirochete."
Fraser C.M., Norris S.J., Weinstock G.M., White O., Sutton G.G., Dodson R.J., Gwinn M.L., Hickey E.K., Clayton R.A., Ketchum K.A., Sodergren E., Hardham J.M., McLeod M.P., Salzberg S.L., Peterson J.D., Khalak H.G., Richardson D.L., Howell J.K. expand/collapse author list , Chidambaram M., Utterback T.R., McDonald L.A., Artiach P., Bowman C., Cotton M.D., Fujii C., Garland S.A., Hatch B., Horst K., Roberts K.M., Sandusky M., Weidman J.F., Smith H.O., Venter J.C.
Science 281:375-388(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Nichols.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U61535 Genomic DNA. Translation: AAB39981.1.
AE000520 Genomic DNA. Translation: AAC65336.1.
PIRF71336.
RefSeqNP_218791.1. NC_000919.1.
YP_008091203.1. NC_021490.2.

3D structure databases

ProteinModelPortalP74936.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP74936. 5 interactions.
STRING243276.TP0351.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC65336; AAC65336; TP_0351.
GeneID15851642.
2611465.
KEGGtpa:TP0351.
tpw:TPANIC_0351.
PATRIC20530695. VBITrePal57110_0368.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0263.
KOK00931.
OMAQENEEFA.
OrthoDBEOG6PGK7G.

Enzyme and pathway databases

BioCycTPAL243276:GC1H-376-MONOMER.
UniPathwayUPA00098; UER00359.

Family and domain databases

Gene3D3.40.1160.10. 1 hit.
HAMAPMF_00456. ProB.
InterProIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
[Graphical view]
PfamPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFPIRSF000729. GK. 1 hit.
PRINTSPR00474. GLU5KINASE.
SUPFAMSSF53633. SSF53633. 1 hit.
TIGRFAMsTIGR01027. proB. 1 hit.
PROSITEPS00902. GLUTAMATE_5_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePROB_TREPA
AccessionPrimary (citable) accession number: P74936
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: May 14, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways