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Protein

Glutamate 5-kinase

Gene

proB

Organism
Treponema pallidum (strain Nichols)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei15 – 151ATPUniRule annotation
Binding sitei55 – 551SubstrateUniRule annotation
Binding sitei159 – 1591SubstrateUniRule annotation
Binding sitei186 – 1861Substrate; via amide nitrogenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi206 – 2072ATPUniRule annotation
Nucleotide bindingi248 – 2547ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate 5-kinase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. L-proline biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTPAL243276:GC1H-376-MONOMER.
UniPathwayiUPA00098; UER00359.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:TP_0351
OrganismiTreponema pallidum (strain Nichols)
Taxonomic identifieri243276 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeTreponema
ProteomesiUP000000811: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 296296Glutamate 5-kinasePRO_0000109750Add
BLAST

Interactioni

Protein-protein interaction databases

IntActiP74936. 5 interactions.
STRINGi243276.TP0351.

Structurei

3D structure databases

ProteinModelPortaliP74936.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0263.
KOiK00931.
OMAiVEVVCQG.
OrthoDBiEOG6PGK7G.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P74936-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIRALFAAAK KIVIKIGSNT LAQADGTPDE EFLAECARAC AALMRDGKQI
60 70 80 90 100
VVVSSGAQVA GISALHCLSS PPQGAGLERH ESRGVIPGDG ASCKQALCAV
110 120 130 140 150
GQAELISRWR SAFAAHQQCV GQFLCTKEDF TDSDRAAQVR YTLSFLLERR
160 170 180 190 200
VVPILNENDA LCCSDVPSVP ADRRVSLSPQ KRIGDNDSLS AFVALLWQAD
210 220 230 240 250
LLLLLSDIDG VYDKDPKAHT DAQHVPLVTD VSALVGKTSM GSSNVFGTGG
260 270 280 290
IATKLDAARL VTRAGIPLVL ANGRHLDPIL SLMRGDARGT LFVPVS
Length:296
Mass (Da):31,315
Last modified:February 1, 1997 - v1
Checksum:i4276927F287E4F14
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U61535 Genomic DNA. Translation: AAB39981.1.
AE000520 Genomic DNA. Translation: AAC65336.1.
PIRiF71336.
RefSeqiNP_218791.1. NC_000919.1.
WP_010881799.1. NC_021490.2.
YP_008091203.1. NC_021490.2.

Genome annotation databases

EnsemblBacteriaiAAC65336; AAC65336; TP_0351.
GeneIDi15851642.
2611465.
KEGGitpa:TP0351.
tpw:TPANIC_0351.
PATRICi20530695. VBITrePal57110_0368.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U61535 Genomic DNA. Translation: AAB39981.1.
AE000520 Genomic DNA. Translation: AAC65336.1.
PIRiF71336.
RefSeqiNP_218791.1. NC_000919.1.
WP_010881799.1. NC_021490.2.
YP_008091203.1. NC_021490.2.

3D structure databases

ProteinModelPortaliP74936.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP74936. 5 interactions.
STRINGi243276.TP0351.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC65336; AAC65336; TP_0351.
GeneIDi15851642.
2611465.
KEGGitpa:TP0351.
tpw:TPANIC_0351.
PATRICi20530695. VBITrePal57110_0368.

Phylogenomic databases

eggNOGiCOG0263.
KOiK00931.
OMAiVEVVCQG.
OrthoDBiEOG6PGK7G.

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.
BioCyciTPAL243276:GC1H-376-MONOMER.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequences of the proA and proB genes of Treponema pallidum, the syphilis agent."
    Stamm L.V., Barnes N.Y.
    DNA Seq. 8:63-70(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Nichols.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Nichols.

Entry informationi

Entry nameiPROB_TREPA
AccessioniPrimary (citable) accession number: P74936
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: January 7, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.