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Reviewed, UniProtKB/Swiss-Prot P74881 (PUR4_SALTY)

Last modified November 3, 2009. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Phosphoribosylformylglycinamidine synthase
      Short name=FGAM synthase
      Short name=FGAMS
    EC=6.3.5.3
Alternative name(s):
    Formylglycinamide ribotide amidotransferase
      Short name=FGARAT
    Formylglycinamide ribotide synthetase
Gene names
Name: purL
Ordered Locus Names: STM2565
OrganismSalmonella typhimurium [Complete proteome] [HAMAP]
Taxonomic identifier90371 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

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Protein attributes

Sequence length1295 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Catalytic activity

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419

Subunit structure

Monomer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

In the N-terminal section; belongs to the FGAMS family.

Contains 1 glutamine amidotransferase type-1 domain.

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Ontologies

Keywords
   Biological processPurine biosynthesis
   Cellular componentCytoplasm
   DomainGlutamine amidotransferase
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological process'de novo' IMP biosynthetic process

Inferred from electronic annotation. Source: InterPro

glutamine metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: HAMAP

phosphoribosylformylglycinamidine synthase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12951295Phosphoribosylformylglycinamidine synthase HAMAP MF_00419
PRO_0000100418

Regions

Domain1042 – 1295254Glutamine amidotransferase type-1
Nucleotide binding307 – 31812ATP Potential

Sites

Active site11351Nucleophile By similarity
Active site12601 By similarity
Active site12621 By similarity

Secondary structure

1 .................................................................................................................................................................................................................. 1295
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P74881-1 [UniParc].

Last modified December 4, 2007. Version 3.
Checksum: 55072EAD8712040D

FASTA1,295141,472
        10         20         30         40         50         60 
MMEILRGSPA LSAFRINKLL ARFQAANLQV HNIYAEYVHF ADLNAPLNDS EQAQLTRLLQ 

        70         80         90        100        110        120 
YGPALSSHTP AGKLLLVTPR PGTISPWSSK ATDIAHNCGL QQVDRLERGV AYYIEASTLT 

       130        140        150        160        170        180 
AEQWRQVAAE LHDRMMETVF SSLTDAEKLF IHHQPAPVSS VDLLGEGRQA LIDANLRLGL 

       190        200        210        220        230        240 
ALAEDEIDYL QEAFTKLGRN PNDIELYMFA QANSEHCRHK IFNADWIIDG KPQPKSLFKM 

       250        260        270        280        290        300 
IKNTFETTPD YVLSAYKDNA AVMEGSAVGR YFADHNTGRY DFHQEPAHIL MKVETHNHPT 

       310        320        330        340        350        360 
AISPWPGAAT GSGGEIRDEG ATGRGAKPKA GLVGFSVSNL RIPGFEQPWE EDFGKPERIV 

       370        380        390        400        410        420 
TALDIMTEGP LGGAAFNNEF GRPALTGYFR TYEEKVNSHN GEELRGYHKP IMLAGGIGNI 

       430        440        450        460        470        480 
RADHVQKGEI VVGAKLIVLG GPAMNIGLGG GAASSMASGQ SDADLDFASV QRDNPEMERR 

       490        500        510        520        530        540 
CQEVIDRCWQ LGDANPILFI HDVGAGGLSN AMPELVSDGG RGGKFELRDI LSDEPGMSPL 

       550        560        570        580        590        600 
EIWCNESQER YVLAVAADQL PLFDELCKRE RAPYAVIGDA TEEQHLSLHD NHFDNQPIDL 

       610        620        630        640        650        660 
PLDVLLGKTP KMTRDVQTLK AKGDALNRAD ITIADAVKRV LHLPTVAEKT FLVTIGDRTV 

       670        680        690        700        710        720 
TGMVARDQMV GPWQVPVADC AVTTASLDSY YGEAMSIGER APVALLDFAA SARLAVGEAL 

       730        740        750        760        770        780 
TNIAATQIGD IKRIKLSANW MAAAGHPGED AGLYDAVKAV GEELCPQLGL TIPVGKDSMS 

       790        800        810        820        830        840 
MKTRWQEGNE QREMTSPLSL VISAFARVED VRHTLTPQLS TEDNALLLID LGKGHNALGA 

       850        860        870        880        890        900 
TALAQVYRQL GDKPADVRDV AQLKGFYDAM QALVAARKLL AWHDRSDGGL LVTLAEMAFA 

       910        920        930        940        950        960 
GHCGVQVDIA ALGDDHLAAL FNEELGGVIQ VRAEDRDAVE ALLAQYGLAD CVHYLGQALA 

       970        980        990       1000       1010       1020 
GDRFVITAND QTVFSESRTT LRVWWAETTW QMQRLRDNPQ CADQEHEAKA NDTDPGLNVK 

      1030       1040       1050       1060       1070       1080 
LSFDINEDIA APYIATGARP KVAVLREQGV NSHVEMAAAF HRAGFDAIDV HMSDLLGGRI 

      1090       1100       1110       1120       1130       1140 
GLGNFHALVA CGGFSYGDVL GAGEGWAKSI LFNHRVRDEF ETFFHRPQTL ALGVCNGCQM 

      1150       1160       1170       1180       1190       1200 
MSNLRELIPG SELWPRFVRN HSDRFEARFS LVEVTQSPSL LLQGMVGSQM PIAVSHGEGR 

      1210       1220       1230       1240       1250       1260 
VEVRDDAHLA ALESKGLVAL RYVDNFGKVT ETYPANPNGS PNGITAVTTE NGRVTIMMPH 

      1270       1280       1290 
PERVFRTVAN SWHPENWGED SPWMRIFRNA RKQLG

« Hide

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References

« Hide 'large scale' references
[1]Zilles J.L., Downs D.M.
Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: LT2.
[2]"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2."
McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. expand/collapse author list , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K.
Nature 413:852-856(2001) [PubMed: 11677609] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LT2 / SGSC1412 / ATCC 700720.
+Additional computationally mapped references.

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Cross-references

Sequence databases

U68728 Genomic DNA. Translation: AAB08888.1.
AE006468 Genomic DNA. Translation: AAL21459.1.
RefSeqNP_461500.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1T3TX-ray1.90A1-1295[»]
ModBaseSearch...

Proteomic databases

PRIDEP74881.

Genome annotation databases

GeneID1254087.
GenomeReviewsGene locus STM2565 in contig AE006468_GR.
KEGGstm:STM2565.
NMPDRfig|99287.1.peg.2479.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMP74881.
OMAERGIAYY.

Enzyme and pathway databases

BioCycSTYP99287:STM2565-MON.
BRENDA6.3.5.3. 2.

Family and domain databases

HAMAPMF_00419.
[Tree]
InterProIPR000728. AIR_synth.
IPR010918. AIR_synth_C.
IPR017926. GATASE_1.
IPR010073. PRibForGlyAmidine_synth.
[Graphical view]
PfamPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 2 hits.
[Graphical view]
TIGRFAMsTIGR01735. FGAM_synt. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry namePUR4_SALTY
AccessionPrimary (citable) accession number: P74881
Entry history
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: December 4, 2007
Last modified: November 3, 2009
This is version 89 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents