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P74840 (PRPD_SALTY) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
2-methylcitrate dehydratase

EC=4.2.1.79
Gene names
Name:prpD
Ordered Locus Names:STM0370
OrganismSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]
Taxonomic identifier99287 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Protein attributes

Sequence length483 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the dehydration of 2-methylcitrate to 2-methyl-cis-aconitate. Ref.3

Catalytic activity

(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate = (Z)-but-2-ene-1,2,3-tricarboxylate + H2O.

Cofactor

Binds 1 2Fe-2S cluster By similarity.

Pathway

Organic acid metabolism; propanoate degradation. Ref.3

Subunit structure

Monomer By similarity.

Sequence similarities

Belongs to the PrpD family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4834832-methylcitrate dehydratase
PRO_0000215026

Experimental info

Sequence conflict3081Q → H in AAC44816. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P74840 [UniParc].

Last modified January 23, 2002. Version 2.
Checksum: CF045F6958157467

FASTA48353,787
        10         20         30         40         50         60 
MSTQELNIRP DFDREIVDIV DYVMNYEITS KVAYDTAHYC LLDTLGCGLE ALEYPACKKL 

        70         80         90        100        110        120 
LGPIVPGTVV PNGARVPGTQ FQLDPVQAAF NIGAMIRWLD FNDTWLAAEW GHPSDNLGGI 

       130        140        150        160        170        180 
LATADWLSRN AVAAGKAPLT MKQVLSGMIK AHEIQGCIAL ENAFNRVGLD HVLLVKVAST 

       190        200        210        220        230        240 
AVVAEMLGLT RDEILNAVSL AWVDGQSLRT YRHAPNTGTR KSWAAGDATS RAVRLALMAK 

       250        260        270        280        290        300 
TGEMGYPSAL TAKTWGFYDV SFKGETFRFQ RPYGSYVMEN VLFKISFPAE FHSQTAVEAA 

       310        320        330        340        350        360 
MTLYEQMQAA GKTAADIEKV TIRTHEACLR IIDKKGPLNN PADRDHCIQY MVAVPLLFGR 

       370        380        390        400        410        420 
LTAADYEDEV AQDKRIDALR EKIVCYEDPA FTADYHDPEK RAIGNAITVE FTDGSRFGEV 

       430        440        450        460        470        480 
VVEYPIGHAR RRADGIPKLI EKFKINLARQ FPTRQQQRIL DVSLDRARLE QMPVNEYLDL 


YVI 

« Hide

References

« Hide 'large scale' references
[1]"Propionate catabolism in Salmonella typhimurium LT2: two divergently transcribed units comprise the prp locus at 8.5 centisomes, prpR encodes a member of the sigma-54 family of activators, and the prpBCDE genes constitute an operon."
Horswill A.R., Escalante-Semerena J.C.
J. Bacteriol. 179:928-940(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: LT2.
[2]"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2."
McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. expand/collapse author list , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K.
Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LT2 / SGSC1412 / ATCC 700720.
[3]"In vitro conversion of propionate to pyruvate by Salmonella enterica enzymes: 2-methylcitrate dehydratase (PrpD) and aconitase enzymes catalyze the conversion of 2-methylcitrate to 2-methylisocitrate."
Horswill A.R., Escalante-Semerena J.C.
Biochemistry 40:4703-4713(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, PATHWAY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U51879 Genomic DNA. Translation: AAC44816.1.
AE006468 Genomic DNA. Translation: AAL19324.1.
RefSeqNP_459365.1. NC_003197.1.

3D structure databases

ProteinModelPortalP74840.
SMRP74840. Positions 11-483.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING99287.STM0370.

Proteomic databases

PaxDbP74840.
PRIDEP74840.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL19324; AAL19324; STM0370.
GeneID1251889.
KEGGstm:STM0370.
PATRIC32379061. VBISalEnt20916_0392.

Phylogenomic databases

eggNOGCOG2079.
HOGENOMHOG000159916.
KOK01720.
OMATAPQWGF.
OrthoDBEOG6RC3K5.
PhylomeDBP74840.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-64.
SENT99287:GCTI-371-MONOMER.
UniPathwayUPA00946.

Family and domain databases

InterProIPR012705. 2Me_IsoCit_deHydtase_PrpD.
IPR005656. MmgE_PrpD.
[Graphical view]
PANTHERPTHR16943. PTHR16943. 1 hit.
PfamPF03972. MmgE_PrpD. 1 hit.
[Graphical view]
SUPFAMSSF103378. SSF103378. 1 hit.
TIGRFAMsTIGR02330. prpD. 1 hit.
ProtoNetSearch...

Entry information

Entry namePRPD_SALTY
AccessionPrimary (citable) accession number: P74840
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2002
Last modified: July 9, 2014
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways