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P74840

- PRPD_SALTY

UniProt

P74840 - PRPD_SALTY

Protein

2-methylcitrate dehydratase

Gene

prpD

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 2 (23 Jan 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the dehydration of 2-methylcitrate to 2-methyl-cis-aconitate.1 Publication

    Catalytic activityi

    (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate = (Z)-but-2-ene-1,2,3-tricarboxylate + H2O.

    Cofactori

    Binds 1 2Fe-2S cluster.By similarity

    Pathwayi

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 2-methylcitrate dehydratase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. propionate catabolic process, 2-methylcitrate cycle Source: InterPro

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    2Fe-2S, Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-64.
    SENT99287:GCTI-371-MONOMER.
    UniPathwayiUPA00946.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2-methylcitrate dehydratase (EC:4.2.1.79)
    Gene namesi
    Name:prpD
    Ordered Locus Names:STM0370
    OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
    Taxonomic identifieri99287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
    ProteomesiUP000001014: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 4834832-methylcitrate dehydratasePRO_0000215026Add
    BLAST

    Proteomic databases

    PaxDbiP74840.
    PRIDEiP74840.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi99287.STM0370.

    Structurei

    3D structure databases

    ProteinModelPortaliP74840.
    SMRiP74840. Positions 11-483.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PrpD family.Curated

    Phylogenomic databases

    eggNOGiCOG2079.
    HOGENOMiHOG000159916.
    KOiK01720.
    OMAiTAPQWGF.
    OrthoDBiEOG6RC3K5.
    PhylomeDBiP74840.

    Family and domain databases

    InterProiIPR012705. 2Me_IsoCit_deHydtase_PrpD.
    IPR005656. MmgE_PrpD.
    [Graphical view]
    PANTHERiPTHR16943. PTHR16943. 1 hit.
    PfamiPF03972. MmgE_PrpD. 1 hit.
    [Graphical view]
    SUPFAMiSSF103378. SSF103378. 1 hit.
    TIGRFAMsiTIGR02330. prpD. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P74840-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTQELNIRP DFDREIVDIV DYVMNYEITS KVAYDTAHYC LLDTLGCGLE    50
    ALEYPACKKL LGPIVPGTVV PNGARVPGTQ FQLDPVQAAF NIGAMIRWLD 100
    FNDTWLAAEW GHPSDNLGGI LATADWLSRN AVAAGKAPLT MKQVLSGMIK 150
    AHEIQGCIAL ENAFNRVGLD HVLLVKVAST AVVAEMLGLT RDEILNAVSL 200
    AWVDGQSLRT YRHAPNTGTR KSWAAGDATS RAVRLALMAK TGEMGYPSAL 250
    TAKTWGFYDV SFKGETFRFQ RPYGSYVMEN VLFKISFPAE FHSQTAVEAA 300
    MTLYEQMQAA GKTAADIEKV TIRTHEACLR IIDKKGPLNN PADRDHCIQY 350
    MVAVPLLFGR LTAADYEDEV AQDKRIDALR EKIVCYEDPA FTADYHDPEK 400
    RAIGNAITVE FTDGSRFGEV VVEYPIGHAR RRADGIPKLI EKFKINLARQ 450
    FPTRQQQRIL DVSLDRARLE QMPVNEYLDL YVI 483
    Length:483
    Mass (Da):53,787
    Last modified:January 23, 2002 - v2
    Checksum:iCF045F6958157467
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti308 – 3081Q → H in AAC44816. (PubMed:9006051)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U51879 Genomic DNA. Translation: AAC44816.1.
    AE006468 Genomic DNA. Translation: AAL19324.1.
    RefSeqiNP_459365.1. NC_003197.1.

    Genome annotation databases

    EnsemblBacteriaiAAL19324; AAL19324; STM0370.
    GeneIDi1251889.
    KEGGistm:STM0370.
    PATRICi32379061. VBISalEnt20916_0392.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U51879 Genomic DNA. Translation: AAC44816.1 .
    AE006468 Genomic DNA. Translation: AAL19324.1 .
    RefSeqi NP_459365.1. NC_003197.1.

    3D structure databases

    ProteinModelPortali P74840.
    SMRi P74840. Positions 11-483.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 99287.STM0370.

    Proteomic databases

    PaxDbi P74840.
    PRIDEi P74840.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL19324 ; AAL19324 ; STM0370 .
    GeneIDi 1251889.
    KEGGi stm:STM0370.
    PATRICi 32379061. VBISalEnt20916_0392.

    Phylogenomic databases

    eggNOGi COG2079.
    HOGENOMi HOG000159916.
    KOi K01720.
    OMAi TAPQWGF.
    OrthoDBi EOG6RC3K5.
    PhylomeDBi P74840.

    Enzyme and pathway databases

    UniPathwayi UPA00946 .
    BioCyci MetaCyc:MONOMER-64.
    SENT99287:GCTI-371-MONOMER.

    Family and domain databases

    InterProi IPR012705. 2Me_IsoCit_deHydtase_PrpD.
    IPR005656. MmgE_PrpD.
    [Graphical view ]
    PANTHERi PTHR16943. PTHR16943. 1 hit.
    Pfami PF03972. MmgE_PrpD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103378. SSF103378. 1 hit.
    TIGRFAMsi TIGR02330. prpD. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Propionate catabolism in Salmonella typhimurium LT2: two divergently transcribed units comprise the prp locus at 8.5 centisomes, prpR encodes a member of the sigma-54 family of activators, and the prpBCDE genes constitute an operon."
      Horswill A.R., Escalante-Semerena J.C.
      J. Bacteriol. 179:928-940(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: LT2.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: LT2 / SGSC1412 / ATCC 700720.
    3. "In vitro conversion of propionate to pyruvate by Salmonella enterica enzymes: 2-methylcitrate dehydratase (PrpD) and aconitase enzymes catalyze the conversion of 2-methylcitrate to 2-methylisocitrate."
      Horswill A.R., Escalante-Semerena J.C.
      Biochemistry 40:4703-4713(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PATHWAY.

    Entry informationi

    Entry nameiPRPD_SALTY
    AccessioniPrimary (citable) accession number: P74840
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: January 23, 2002
    Last modified: October 1, 2014
    This is version 88 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3