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Protein

2-methylcitrate dehydratase

Gene

prpD

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the dehydration of 2-methylcitrate to 2-methyl-cis-aconitate.1 Publication

Catalytic activityi

(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate = (Z)-but-2-ene-1,2,3-tricarboxylate + H2O.

Cofactori

[2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster.By similarity

Pathwayi

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 2-methylcitrate dehydratase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. propionate catabolic process Source: SGD
  2. propionate catabolic process, 2-methylcitrate cycle Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-64.
SENT99287:GCTI-371-MONOMER.
BRENDAi4.2.1.79. 2169.
UniPathwayiUPA00946.

Names & Taxonomyi

Protein namesi
Recommended name:
2-methylcitrate dehydratase (EC:4.2.1.79)
Gene namesi
Name:prpD
Ordered Locus Names:STM0370
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001014 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4834832-methylcitrate dehydratasePRO_0000215026Add
BLAST

Proteomic databases

PaxDbiP74840.
PRIDEiP74840.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi99287.STM0370.

Structurei

3D structure databases

ProteinModelPortaliP74840.
SMRiP74840. Positions 11-483.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PrpD family.Curated

Phylogenomic databases

eggNOGiCOG2079.
HOGENOMiHOG000159916.
KOiK01720.
OMAiCLMDTLG.
OrthoDBiEOG6RC3K5.
PhylomeDBiP74840.

Family and domain databases

InterProiIPR012705. 2Me_IsoCit_deHydtase_PrpD.
IPR005656. MmgE_PrpD.
[Graphical view]
PANTHERiPTHR16943. PTHR16943. 1 hit.
PfamiPF03972. MmgE_PrpD. 1 hit.
[Graphical view]
SUPFAMiSSF103378. SSF103378. 1 hit.
TIGRFAMsiTIGR02330. prpD. 1 hit.

Sequencei

Sequence statusi: Complete.

P74840-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTQELNIRP DFDREIVDIV DYVMNYEITS KVAYDTAHYC LLDTLGCGLE
60 70 80 90 100
ALEYPACKKL LGPIVPGTVV PNGARVPGTQ FQLDPVQAAF NIGAMIRWLD
110 120 130 140 150
FNDTWLAAEW GHPSDNLGGI LATADWLSRN AVAAGKAPLT MKQVLSGMIK
160 170 180 190 200
AHEIQGCIAL ENAFNRVGLD HVLLVKVAST AVVAEMLGLT RDEILNAVSL
210 220 230 240 250
AWVDGQSLRT YRHAPNTGTR KSWAAGDATS RAVRLALMAK TGEMGYPSAL
260 270 280 290 300
TAKTWGFYDV SFKGETFRFQ RPYGSYVMEN VLFKISFPAE FHSQTAVEAA
310 320 330 340 350
MTLYEQMQAA GKTAADIEKV TIRTHEACLR IIDKKGPLNN PADRDHCIQY
360 370 380 390 400
MVAVPLLFGR LTAADYEDEV AQDKRIDALR EKIVCYEDPA FTADYHDPEK
410 420 430 440 450
RAIGNAITVE FTDGSRFGEV VVEYPIGHAR RRADGIPKLI EKFKINLARQ
460 470 480
FPTRQQQRIL DVSLDRARLE QMPVNEYLDL YVI
Length:483
Mass (Da):53,787
Last modified:January 23, 2002 - v2
Checksum:iCF045F6958157467
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti308 – 3081Q → H in AAC44816 (PubMed:9006051).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51879 Genomic DNA. Translation: AAC44816.1.
AE006468 Genomic DNA. Translation: AAL19324.1.
RefSeqiNP_459365.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL19324; AAL19324; STM0370.
GeneIDi1251889.
KEGGistm:STM0370.
PATRICi32379061. VBISalEnt20916_0392.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51879 Genomic DNA. Translation: AAC44816.1.
AE006468 Genomic DNA. Translation: AAL19324.1.
RefSeqiNP_459365.1. NC_003197.1.

3D structure databases

ProteinModelPortaliP74840.
SMRiP74840. Positions 11-483.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM0370.

Proteomic databases

PaxDbiP74840.
PRIDEiP74840.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL19324; AAL19324; STM0370.
GeneIDi1251889.
KEGGistm:STM0370.
PATRICi32379061. VBISalEnt20916_0392.

Phylogenomic databases

eggNOGiCOG2079.
HOGENOMiHOG000159916.
KOiK01720.
OMAiCLMDTLG.
OrthoDBiEOG6RC3K5.
PhylomeDBiP74840.

Enzyme and pathway databases

UniPathwayiUPA00946.
BioCyciMetaCyc:MONOMER-64.
SENT99287:GCTI-371-MONOMER.
BRENDAi4.2.1.79. 2169.

Family and domain databases

InterProiIPR012705. 2Me_IsoCit_deHydtase_PrpD.
IPR005656. MmgE_PrpD.
[Graphical view]
PANTHERiPTHR16943. PTHR16943. 1 hit.
PfamiPF03972. MmgE_PrpD. 1 hit.
[Graphical view]
SUPFAMiSSF103378. SSF103378. 1 hit.
TIGRFAMsiTIGR02330. prpD. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Propionate catabolism in Salmonella typhimurium LT2: two divergently transcribed units comprise the prp locus at 8.5 centisomes, prpR encodes a member of the sigma-54 family of activators, and the prpBCDE genes constitute an operon."
    Horswill A.R., Escalante-Semerena J.C.
    J. Bacteriol. 179:928-940(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LT2.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.
  3. "In vitro conversion of propionate to pyruvate by Salmonella enterica enzymes: 2-methylcitrate dehydratase (PrpD) and aconitase enzymes catalyze the conversion of 2-methylcitrate to 2-methylisocitrate."
    Horswill A.R., Escalante-Semerena J.C.
    Biochemistry 40:4703-4713(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PATHWAY.

Entry informationi

Entry nameiPRPD_SALTY
AccessioniPrimary (citable) accession number: P74840
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2002
Last modified: April 1, 2015
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.