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Protein

Elongation factor 4

Gene

lepA

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.UniRule annotation

Catalytic activityi

GTP + H2O = GDP + phosphate.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi19 – 24GTPUniRule annotation6
Nucleotide bindingi136 – 139GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 4UniRule annotation (EC:3.6.5.n1UniRule annotation)
Short name:
EF-4UniRule annotation
Alternative name(s):
Ribosomal back-translocase LepAUniRule annotation
Gene namesi
Name:lepAUniRule annotation
Ordered Locus Names:slr0604
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesMerismopediaceaeSynechocystis
Proteomesi
  • UP000001425 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001763611 – 603Elongation factor 4Add BLAST603

Proteomic databases

PRIDEiP74751.

Interactioni

Protein-protein interaction databases

IntActiP74751. 3 interactors.

Structurei

3D structure databases

ProteinModelPortaliP74751.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 189tr-type GAdd BLAST183

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000020624.
InParanoidiP74751.
KOiK03596.
OMAiFAGIYPV.
PhylomeDBiP74751.

Family and domain databases

Gene3Di3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_03138. GUFP. 1 hit.
MF_00071. LepA. 1 hit.
InterProiIPR006297. EF-4.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027518. GUFP.
IPR013842. LepA_GTP-bd_C.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P74751-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDVPVSRIR NFSIIAHIDH GKSTLADRLL QVTDTVQQRE MKEQFLDNMD
60 70 80 90 100
LERERGITIK LQAARMNYKA KDGQDYVLNL IDTPGHVDFS YEVSRSLAAC
110 120 130 140 150
EGALLVVDAS QGVEAQTLAN VYLALDNNLE IIPVLNKIDL PSAEPDRVAA
160 170 180 190 200
EIEEVVGLDC SDIIQASAKA GIGVDDILEA IVQQVPPPAD TVDQPLRALI
210 220 230 240 250
FDSYYDAYRG VVVYFRVMDG QVKKGDKVRL MASDKEYVID ELGVLSPTQV
260 270 280 290 300
QVEALHAGEV GYFAAAIKAV ADARVGDTIT MANSPAAEPL PGYTEANPMV
310 320 330 340 350
FCGLFPIDAD QYPDLKDALE KLKLNDAALS YEPETSSAMG FGFRCGFLGL
360 370 380 390 400
LHMEIVQERL EREYNLDLIT TAPSVIYRVT TTDEEVIEVD NPSLLPPIQK
410 420 430 440 450
RLKVEEPFIK VEMITPETYV GTLMELCQSR RGVFKDMKFF TQTRTALIYE
460 470 480 490 500
LPLAEVVTDF FDQLKSRTKG YASMEYQLIG YRENPLVKLD ILVNGDGVDA
510 520 530 540 550
LAMIVHRDKA YYVGRAMVSK LKELIPRHQF KVPIQAAIGA KVIASEHIPA
560 570 580 590 600
LRKDVLAKCY GGDISRKKKL LQKQAKGKKR MKAIGTVDVP QEAFMAVLKL

DPQ
Length:603
Mass (Da):66,968
Last modified:February 1, 1997 - v1
Checksum:i27AE0D610529AD9B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA18871.1.
PIRiS76959.

Genome annotation databases

EnsemblBacteriaiBAA18871; BAA18871; BAA18871.
KEGGisyn:slr0604.
PATRICi23843976. VBISynSp132158_3518.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA18871.1.
PIRiS76959.

3D structure databases

ProteinModelPortaliP74751.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP74751. 3 interactors.

Proteomic databases

PRIDEiP74751.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA18871; BAA18871; BAA18871.
KEGGisyn:slr0604.
PATRICi23843976. VBISynSp132158_3518.

Phylogenomic databases

HOGENOMiHOG000020624.
InParanoidiP74751.
KOiK03596.
OMAiFAGIYPV.
PhylomeDBiP74751.

Family and domain databases

Gene3Di3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_03138. GUFP. 1 hit.
MF_00071. LepA. 1 hit.
InterProiIPR006297. EF-4.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027518. GUFP.
IPR013842. LepA_GTP-bd_C.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLEPA_SYNY3
AccessioniPrimary (citable) accession number: P74751
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.