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Protein

Elongation factor 4

Gene

lepA

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.UniRule annotation

Catalytic activityi

GTP + H2O = GDP + phosphate.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi19 – 24GTPUniRule annotation6
Nucleotide bindingi136 – 139GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 4UniRule annotation (EC:3.6.5.n1UniRule annotation)
Short name:
EF-4UniRule annotation
Alternative name(s):
Ribosomal back-translocase LepAUniRule annotation
Gene namesi
Name:lepAUniRule annotation
Ordered Locus Names:slr0604
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesMerismopediaceaeSynechocystis
Proteomesi
  • UP000001425 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001763611 – 603Elongation factor 4Add BLAST603

Proteomic databases

PaxDbiP74751
PRIDEiP74751

Interactioni

Protein-protein interaction databases

IntActiP74751, 3 interactors
STRINGi1148.SYNGTS_3165

Structurei

3D structure databases

ProteinModelPortaliP74751
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 189tr-type GAdd BLAST183

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000020624
InParanoidiP74751
KOiK03596
OMAiALIFDSW
PhylomeDBiP74751

Family and domain databases

CDDicd03709 lepA_C, 1 hit
Gene3Di3.30.70.2570, 1 hit
HAMAPiMF_03138 GUFP, 1 hit
MF_00071 LepA, 1 hit
InterProiView protein in InterPro
IPR006297 EF-4
IPR035647 EFG_III/V
IPR000640 EFG_V-like
IPR004161 EFTu-like_2
IPR031157 G_TR_CS
IPR027518 GUFP
IPR038363 LepA_C_sf
IPR013842 LepA_CTD
IPR035654 LepA_IV
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR000795 TF_GTP-bd_dom
PfamiView protein in Pfam
PF00679 EFG_C, 1 hit
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PF06421 LepA_C, 1 hit
PRINTSiPR00315 ELONGATNFCT
SMARTiView protein in SMART
SM00838 EFG_C, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
SSF54980 SSF54980, 2 hits
TIGRFAMsiTIGR01393 lepA, 1 hit
TIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

Sequencei

Sequence statusi: Complete.

P74751-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDVPVSRIR NFSIIAHIDH GKSTLADRLL QVTDTVQQRE MKEQFLDNMD
60 70 80 90 100
LERERGITIK LQAARMNYKA KDGQDYVLNL IDTPGHVDFS YEVSRSLAAC
110 120 130 140 150
EGALLVVDAS QGVEAQTLAN VYLALDNNLE IIPVLNKIDL PSAEPDRVAA
160 170 180 190 200
EIEEVVGLDC SDIIQASAKA GIGVDDILEA IVQQVPPPAD TVDQPLRALI
210 220 230 240 250
FDSYYDAYRG VVVYFRVMDG QVKKGDKVRL MASDKEYVID ELGVLSPTQV
260 270 280 290 300
QVEALHAGEV GYFAAAIKAV ADARVGDTIT MANSPAAEPL PGYTEANPMV
310 320 330 340 350
FCGLFPIDAD QYPDLKDALE KLKLNDAALS YEPETSSAMG FGFRCGFLGL
360 370 380 390 400
LHMEIVQERL EREYNLDLIT TAPSVIYRVT TTDEEVIEVD NPSLLPPIQK
410 420 430 440 450
RLKVEEPFIK VEMITPETYV GTLMELCQSR RGVFKDMKFF TQTRTALIYE
460 470 480 490 500
LPLAEVVTDF FDQLKSRTKG YASMEYQLIG YRENPLVKLD ILVNGDGVDA
510 520 530 540 550
LAMIVHRDKA YYVGRAMVSK LKELIPRHQF KVPIQAAIGA KVIASEHIPA
560 570 580 590 600
LRKDVLAKCY GGDISRKKKL LQKQAKGKKR MKAIGTVDVP QEAFMAVLKL

DPQ
Length:603
Mass (Da):66,968
Last modified:February 1, 1997 - v1
Checksum:i27AE0D610529AD9B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA Translation: BAA18871.1
PIRiS76959

Genome annotation databases

EnsemblBacteriaiBAA18871; BAA18871; BAA18871
KEGGisyn:slr0604

Similar proteinsi

Entry informationi

Entry nameiLEPA_SYNY3
AccessioniPrimary (citable) accession number: P74751
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

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