ID DPO3A_SYNY3 Reviewed; 1355 AA. AC P74750; P73215; DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot. DT 26-SEP-2001, sequence version 2. DT 22-SEP-2009, entry version 76. DE RecName: Full=DNA polymerase III subunit alpha; DE EC=2.7.7.7; DE Contains: DE RecName: Full=Ssp dnaE intein; DE Flags: Fragments; GN Name=dnaE-N; OrderedLocusNames=slr0603; GN and GN Name=dnaE-C; OrderedLocusNames=sll1572; OS Synechocystis sp. (strain PCC 6803). OC Bacteria; Cyanobacteria; Chroococcales; Synechocystis. OX NCBI_TaxID=1148; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX MEDLINE=97061201; PubMed=8905231; DOI=10.1093/dnares/3.3.109; RA Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., RA Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., RA Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., RA Shimpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., RA Tabata S.; RT "Sequence analysis of the genome of the unicellular cyanobacterium RT Synechocystis sp. strain PCC6803. II. Sequence determination of the RT entire genome and assignment of potential protein-coding regions."; RL DNA Res. 3:109-136(1996). RN [2] RP CHARACTERIZATION OF SPLIT INTEIN. RX MEDLINE=98356139; PubMed=9689062; DOI=10.1073/pnas.95.16.9226; RA Wu H., Hu Z., Liu X.-Q.; RT "Protein trans-splicing by a split intein encoded in a split DnaE gene RT of Synechocystis sp. PCC6803."; RL Proc. Natl. Acad. Sci. U.S.A. 95:9226-9231(1998). RN [3] RP CHARACTERIZATION OF SPLIT INTEIN. RX MEDLINE=20200384; PubMed=10734038; DOI=10.1074/jbc.275.13.9091; RA Evans T.C. Jr., Martin D., Kolly R., Panne D., Sun L., Ghosh I., RA Chen L., Benner J., Liu X.-Q., Xu M.-Q.; RT "Protein trans-splicing and cyclization by a naturally split intein RT from the dnaE gene of Synechocystis species PCC6803."; RL J. Biol. Chem. 275:9091-9094(2000). RN [4] RP CHARACTERIZATION OF SPLIT INTEIN. RX MEDLINE=21093115; PubMed=11170467; DOI=10.1021/bi001786g; RA Martin D.D., Xu M.-Q., Evans T.C. Jr.; RT "Characterization of a naturally occurring trans-splicing intein from RT Synechocystis sp. PCC6803."; RL Biochemistry 40:1393-1402(2001). CC -!- FUNCTION: DNA polymerase III is a complex, multichain enzyme CC responsible for most of the replicative synthesis in bacteria. CC This DNA polymerase also exhibits 3' to 5' exonuclease activity. CC The alpha chain is the DNA polymerase (By similarity). CC -!- CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) = CC diphosphate + DNA(n+1). CC -!- SUBUNIT: DNA polymerase III contains a core (composed of alpha, CC epsilon and theta chains) that associates with a tau subunit. This CC core dimerizes to form the PolIII' complex. PolIII' associates CC with the gamma complex (composed of gamma, delta, delta', psi and CC chi chains) and with the beta chain to form the complete DNA CC polymerase III complex (By similarity). CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity). CC -!- PTM: This protein undergoes a protein self splicing that involves CC a post-translational excision of the intervening region (intein) CC followed by peptide ligation. CC -!- MISCELLANEOUS: The intein is a split intein capable of protein CC trans-splicing. The N- and C-terminal halves of dnaE are encoded CC by two separate genes located 745 kb apart in the genome and on CC opposite DNA strands. The dnaE-N product consists of a N-extein CC sequence followed by a 123-aa intein sequence (Ssp dnaE1), whereas CC the dnaE-C product consists of a 36-aa intein sequence (Ssp dnaE2) CC followed by a C-extein sequence. The N- and C-extein sequences CC together reconstitute a complete DnaE sequence that is interrupted CC by the intein sequences inside the beta- and tau-binding domains. CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE CC subfamily. CC ----------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution-NoDerivs License CC ----------------------------------------------------------------------- DR EMBL; BA000022; BAA18870.1; ALT_SEQ; Genomic_DNA. DR EMBL; BA000022; BAA17242.1; ALT_SEQ; Genomic_DNA. DR PIR; A59016; A59016. DR PIR; S75328; S75328. DR PIR; S76958; S76958. DR RefSeq; NP_440562.1; -. DR RefSeq; NP_443058.1; -. DR PDB; 1ZD7; X-ray; 1.70 A; A/B=775-933. DR PDB; 1ZDE; X-ray; 1.95 A; A=770-936. DR PDBsum; 1ZD7; -. DR PDBsum; 1ZDE; -. DR IntAct; P74750; 2. DR STRING; P74750; -. DR GeneID; 951978; -. DR GeneID; 953863; -. DR GenomeReviews; BA000022_GR; sll1572. DR GenomeReviews; BA000022_GR; slr0603. DR KEGG; syn:sll1572; -. DR KEGG; syn:slr0603; -. DR HOGENOM; P74750; -. DR BioCyc; SSP1148:SLL1572-MON; -. DR BioCyc; SSP1148:SLR0603-MON; -. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro. DR GO; GO:0003677; F:DNA binding; IEA:InterPro. DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW. DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW. DR GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro. DR InterPro; IPR011708; DNA_pol3_alpha. DR InterPro; IPR003587; Hedgehog_hint_N. DR InterPro; IPR006141; Intein_splicing_site. DR InterPro; IPR004365; NA_bd_OB_tRNA-helicase. DR InterPro; IPR004013; PHP_C. DR InterPro; IPR003141; Pol/His_phosphatase_N. DR InterPro; IPR004805; PolC_alpha. DR Pfam; PF07733; DNA_pol3_alpha; 1. DR Pfam; PF02811; PHP; 1. DR Pfam; PF01336; tRNA_anti; 1. DR SMART; SM00306; HintN; 1. DR SMART; SM00481; POLIIIAc; 1. DR TIGRFAMs; TIGR01443; intein_Cterm; 1. DR TIGRFAMs; TIGR01445; intein_Nterm; 1. DR TIGRFAMs; TIGR00594; polc; 1. DR PROSITE; PS50818; INTEIN_C_TER; 1. DR PROSITE; PS50817; INTEIN_N_TER; 1. PE 1: Evidence at protein level; KW 3D-structure; Autocatalytic cleavage; Complete proteome; Cytoplasm; KW DNA replication; DNA-directed DNA polymerase; Nucleotidyltransferase; KW Protein splicing; Transferase. FT CHAIN 1 774 DNA polymerase III subunit alpha, 1st FT part. FT /FTId=PRO_0000007364. FT CHAIN 775 897 Ssp dnaE intein, 1st part. FT /FTId=PRO_0000007365. FT CHAIN 898 933 Ssp dnaE intein, 2nd part. FT /FTId=PRO_0000007366. FT CHAIN 934 1355 DNA polymerase III subunit alpha, 2nd FT part. FT /FTId=PRO_0000007367. FT NON_CONS 897 898 FT STRAND 781 784 FT TURN 785 787 FT STRAND 788 791 FT HELIX 792 797 FT STRAND 803 807 FT STRAND 811 817 FT STRAND 820 834 FT STRAND 839 842 FT STRAND 847 850 FT STRAND 855 857 FT HELIX 858 863 FT STRAND 867 872 FT HELIX 874 882 FT STRAND 886 889 FT HELIX 890 893 FT STRAND 896 916 FT STRAND 918 925 FT STRAND 928 932 SQ SEQUENCE 1355 AA; 153001 MW; 7C4F61EE4C36EE0F CRC64; MSFVGLHIHS DYSLLDGASQ LPALIDRAIE LGMPAIALTD HGVMYGAVEL LKVCRGKPIK PIIGNEMYVI NGDIEINKRH RRFHQVVLAK NNQGYRNLVK LTTISNLKGI QGSGIFARPC INKELLEQYK EGLIVTSACL GGEVPQAILK GDLDHARQVA KWYKNLFGDD YYLEIQDHGS VEDRLVNINL VKIAQELDIK IVATNDSHFI SCNDVEAHDA LLCIQTGKLI TEEKRLRYSG TEYLKSAEEM HLLFRDHLPD DIIETAIANT LEVADKVEAY SILGEPRIPH YPIPPNHTPE TYVEDIAWDG LLERLKCRER ADITPVYKER LEYELRMLEK MGFCTYFLVV WDYIKYARDH GIPVGPGRGS AAGSLVAYCM KITNIDPVHH GLIFERFLNP ERKSMPDVDT DFCIDRRDEM IEYVTQKYGE DKVAQIITFN RMTSKAVLKD VARVLDIPYA ESDKMAKMIP VSRGKPAKLK VMISDQTPEP QFKARYDNEP WVKKWIDMAI RIEGTNKTFG VHAAGVVISS EPLDQIVPLQ KNNDGAVITQ YYMEDVEAMG LLKMDFLGLK NLTTLQRAVE LVAETKGIEL DLDDLPLQER KALQIRARTG SKKLPDDVKK THKLLEAGDL EGIFQLESQG MKQIVRDLKP SGIEDISSIL ALYRPGPLDA GLIPIFINRK HGREEISYQH KLLEPILNET YGVLVYQEQI MKMAQDLADY SLGEADLLRR AMGKKKAEEM QKHRAKFVDG STKHGVPSRI AENLFDQMVK FAEYCLSFGT EILTVEYGPL PIGKIVSEEI NCSVYSVDPE GRVYTQAIAQ WHDRGEQEVL EYELEDGSVI RATSDHRFLT TDYQLLAIEE IFARQLDLLT LENIKQTEEA LDNHRLPFPL LDAGTIKMVK VIGRRSLGVQ RIFDIGLPQD HNFLLANGAI AANCFNKSHS TAYAYVTYQT AYLKANYPVE YMAALLTASS DSQEKVEKYR ENCQKMGITV EPPDINRSQR HFTPLGEAIL FGLSAVRNLG EGAIEQIITA RDNSEEKRFK SLADFCTQVD LRVVNRRAIE TLIMAGAFDG VQSNRNQLLH DLELVIAWAQ KRAKEKETGQ LNIFDSLTAG ESIKAKEAAN NGFEQEPSAP PVAEFSLQEK LQLEKEHLGF YVSEHPLKSV QRSARLLSPI NLVDLDQYKV RQKVSAVAIL VAVKKIITKK NGQPMAFLTL EDMSGQSEAV VFPSNYERLQ DVLIEGSQQM IWGKVDRRDD QYQLIVEDLE PVEEVKMVML DLTPQEIANT STQARLKQIL QSHAPQKEQM KIPVVACINE GPTKHFIRFG ENYWVNDYGT AVASLQELGF RARLEPLLPQ SQSVG //