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P74750 (DPO3A_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA polymerase III subunit alpha

EC=2.7.7.7

Cleaved into the following chain:

  1. Ssp dnaE intein
Gene names
Name:dnaE-N
Ordered Locus Names:slr0603
AND
Name:dnaE-C
Ordered Locus Names:sll1572
OrganismSynechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]
Taxonomic identifier1111708 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis

Protein attributes

Sequence length1355 AA.
Sequence statusFragments.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase By similarity.

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Subunit structure

DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the PolIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex By similarity.

Subcellular location

Cytoplasm By similarity.

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.

Miscellaneous

The intein is a split intein capable of protein trans-splicing. The N- and C-terminal halves of dnaE are encoded by two separate genes located 745 kb apart in the genome and on opposite DNA strands. The dnaE-N product consists of a N-extein sequence followed by a 123-aa intein sequence (Ssp dnaE1), whereas the dnaE-C product consists of a 36-aa intein sequence (Ssp dnaE2) followed by a C-extein sequence. The N- and C-extein sequences together reconstitute a complete DnaE sequence that is interrupted by the intein sequences inside the beta- and tau-binding domains.

Sequence similarities

Belongs to the DNA polymerase type-C family. DnaE subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 774774DNA polymerase III subunit alpha, 1st part
PRO_0000007364
Chain775 – 897123Ssp dnaE intein, 1st part
PRO_0000007365
Chain898 – 93336Ssp dnaE intein, 2nd part
PRO_0000007366
Chain934 – 1355422DNA polymerase III subunit alpha, 2nd part
PRO_0000007367

Experimental info

Non-adjacent residues897 – 8982

Secondary structure

.................................... 1355
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P74750 [UniParc].

Last modified September 26, 2001. Version 2.
Checksum: 7C4F61EE4C36EE0F

FASTA1,355153,001
        10         20         30         40         50         60 
MSFVGLHIHS DYSLLDGASQ LPALIDRAIE LGMPAIALTD HGVMYGAVEL LKVCRGKPIK 

        70         80         90        100        110        120 
PIIGNEMYVI NGDIEINKRH RRFHQVVLAK NNQGYRNLVK LTTISNLKGI QGSGIFARPC 

       130        140        150        160        170        180 
INKELLEQYK EGLIVTSACL GGEVPQAILK GDLDHARQVA KWYKNLFGDD YYLEIQDHGS 

       190        200        210        220        230        240 
VEDRLVNINL VKIAQELDIK IVATNDSHFI SCNDVEAHDA LLCIQTGKLI TEEKRLRYSG 

       250        260        270        280        290        300 
TEYLKSAEEM HLLFRDHLPD DIIETAIANT LEVADKVEAY SILGEPRIPH YPIPPNHTPE 

       310        320        330        340        350        360 
TYVEDIAWDG LLERLKCRER ADITPVYKER LEYELRMLEK MGFCTYFLVV WDYIKYARDH 

       370        380        390        400        410        420 
GIPVGPGRGS AAGSLVAYCM KITNIDPVHH GLIFERFLNP ERKSMPDVDT DFCIDRRDEM 

       430        440        450        460        470        480 
IEYVTQKYGE DKVAQIITFN RMTSKAVLKD VARVLDIPYA ESDKMAKMIP VSRGKPAKLK 

       490        500        510        520        530        540 
VMISDQTPEP QFKARYDNEP WVKKWIDMAI RIEGTNKTFG VHAAGVVISS EPLDQIVPLQ 

       550        560        570        580        590        600 
KNNDGAVITQ YYMEDVEAMG LLKMDFLGLK NLTTLQRAVE LVAETKGIEL DLDDLPLQER 

       610        620        630        640        650        660 
KALQIRARTG SKKLPDDVKK THKLLEAGDL EGIFQLESQG MKQIVRDLKP SGIEDISSIL 

       670        680        690        700        710        720 
ALYRPGPLDA GLIPIFINRK HGREEISYQH KLLEPILNET YGVLVYQEQI MKMAQDLADY 

       730        740        750        760        770        780 
SLGEADLLRR AMGKKKAEEM QKHRAKFVDG STKHGVPSRI AENLFDQMVK FAEYCLSFGT 

       790        800        810        820        830        840 
EILTVEYGPL PIGKIVSEEI NCSVYSVDPE GRVYTQAIAQ WHDRGEQEVL EYELEDGSVI 

       850        860        870        880        890        900 
RATSDHRFLT TDYQLLAIEE IFARQLDLLT LENIKQTEEA LDNHRLPFPL LDAGTIKMVK 

       910        920        930        940        950        960 
VIGRRSLGVQ RIFDIGLPQD HNFLLANGAI AANCFNKSHS TAYAYVTYQT AYLKANYPVE 

       970        980        990       1000       1010       1020 
YMAALLTASS DSQEKVEKYR ENCQKMGITV EPPDINRSQR HFTPLGEAIL FGLSAVRNLG 

      1030       1040       1050       1060       1070       1080 
EGAIEQIITA RDNSEEKRFK SLADFCTQVD LRVVNRRAIE TLIMAGAFDG VQSNRNQLLH 

      1090       1100       1110       1120       1130       1140 
DLELVIAWAQ KRAKEKETGQ LNIFDSLTAG ESIKAKEAAN NGFEQEPSAP PVAEFSLQEK 

      1150       1160       1170       1180       1190       1200 
LQLEKEHLGF YVSEHPLKSV QRSARLLSPI NLVDLDQYKV RQKVSAVAIL VAVKKIITKK 

      1210       1220       1230       1240       1250       1260 
NGQPMAFLTL EDMSGQSEAV VFPSNYERLQ DVLIEGSQQM IWGKVDRRDD QYQLIVEDLE 

      1270       1280       1290       1300       1310       1320 
PVEEVKMVML DLTPQEIANT STQARLKQIL QSHAPQKEQM KIPVVACINE GPTKHFIRFG 

      1330       1340       1350 
ENYWVNDYGT AVASLQELGF RARLEPLLPQ SQSVG 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. expand/collapse author list , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 6803 / Kazusa.
[2]"Protein trans-splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803."
Wu H., Hu Z., Liu X.-Q.
Proc. Natl. Acad. Sci. U.S.A. 95:9226-9231(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION OF SPLIT INTEIN.
[3]"Protein trans-splicing and cyclization by a naturally split intein from the dnaE gene of Synechocystis species PCC6803."
Evans T.C. Jr., Martin D., Kolly R., Panne D., Sun L., Ghosh I., Chen L., Benner J., Liu X.-Q., Xu M.-Q.
J. Biol. Chem. 275:9091-9094(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION OF SPLIT INTEIN.
[4]"Characterization of a naturally occurring trans-splicing intein from Synechocystis sp. PCC6803."
Martin D.D., Xu M.-Q., Evans T.C. Jr.
Biochemistry 40:1393-1402(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION OF SPLIT INTEIN.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000022 Genomic DNA. Translation: BAA18870.1. Sequence problems.
BA000022 Genomic DNA. Translation: BAA17242.1. Sequence problems.
PIRA59016.
S75328.
S76958.
RefSeqNP_440562.1. NC_000911.1.
NP_443058.1. NC_000911.1.
YP_005650620.1. NC_017277.1.
YP_005653117.1. NC_017277.1.
YP_007450445.1. NC_020286.1.
YP_007452933.1. NC_020286.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZD7X-ray1.70A/B775-933[»]
1ZDEX-ray1.95A770-936[»]
3NZMX-ray1.55A775-933[»]
4GIGX-ray1.80A775-933[»]
ProteinModelPortalP74750.
SMRP74750. Positions 775-933.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP74750. 2 interactions.
STRING1148.sll1572.

Protein family/group databases

MEROPSN10.003.

Proteomic databases

PRIDEP74750.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA17242; BAA17242; BAA17242.
BAA18870; BAA18870; BAA18870.
GeneID12255728.
12256312.
14616095.
14618625.
951978.
953863.
KEGGsyn:sll1572.
syn:slr0603.
syy:SYNGTS_0667.
syy:SYNGTS_3164.
syz:MYO_132000.
syz:MYO_16730.
PATRIC23838310. VBISynSp132158_0722.

Phylogenomic databases

eggNOGCOG0587.
HOGENOMHOG000021784.
KOK02337.
OrthoDBEOG6CZQGR.
PhylomeDBP74750.
ProtClustDBPRK09532.

Family and domain databases

Gene3D2.40.50.140. 1 hit.
InterProIPR011708. DNA_pol3_alpha.
IPR003587. Hint_dom_N.
IPR006141. Intein_splice_site.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
IPR004013. PHP_C.
IPR003141. Pol/His_phosphatase_N.
IPR016195. Pol/histidinol_Pase-like.
IPR004805. PolC_alpha.
[Graphical view]
PfamPF07733. DNA_pol3_alpha. 1 hit.
PF02811. PHP. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
SMARTSM00306. HintN. 1 hit.
SM00481. POLIIIAc. 1 hit.
[Graphical view]
SUPFAMSSF89550. SSF89550. 1 hit.
TIGRFAMsTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR00594. polc. 1 hit.
PROSITEPS50818. INTEIN_C_TER. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP74750.

Entry information

Entry nameDPO3A_SYNY3
AccessionPrimary (citable) accession number: P74750
Secondary accession number(s): P73215
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: September 26, 2001
Last modified: April 16, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Synechocystis PCC 6803

Synechocystis (strain PCC 6803): entries and gene names

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Intein-containing proteins

List of intein-containing protein entries