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P74750

- DPO3A_SYNY3

UniProt

P74750 - DPO3A_SYNY3

Protein

DNA polymerase III subunit alpha

Gene

dnaE-N

more
Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 2 (26 Sep 2001)
      Previous versions | rss
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    Functioni

    DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase By similarity.By similarity

    Catalytic activityi

    Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

    GO - Molecular functioni

    1. 3'-5' exonuclease activity Source: InterPro
    2. DNA binding Source: InterPro
    3. DNA-directed DNA polymerase activity Source: UniProtKB-KW

    GO - Biological processi

    1. intein-mediated protein splicing Source: InterPro

    Keywords - Molecular functioni

    DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    DNA replication

    Protein family/group databases

    MEROPSiN10.003.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA polymerase III subunit alpha (EC:2.7.7.7)
    Cleaved into the following chain:
    Gene namesi
    Name:dnaE-N
    Ordered Locus Names:slr0603
    AND
    Name:dnaE-C
    Ordered Locus Names:sll1572
    OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
    Taxonomic identifieri1111708 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
    ProteomesiUP000001425: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 774774DNA polymerase III subunit alpha, 1st partPRO_0000007364Add
    BLAST
    Chaini775 – 897123Ssp dnaE intein, 1st partPRO_0000007365Add
    BLAST
    Chaini898 – 93336Ssp dnaE intein, 2nd partPRO_0000007366Add
    BLAST
    Chaini934 – 1355422DNA polymerase III subunit alpha, 2nd partPRO_0000007367Add
    BLAST

    Post-translational modificationi

    This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.

    Keywords - PTMi

    Autocatalytic cleavage, Protein splicing

    Proteomic databases

    PRIDEiP74750.

    Interactioni

    Subunit structurei

    DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the PolIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex By similarity.By similarity

    Protein-protein interaction databases

    IntActiP74750. 2 interactions.
    STRINGi1148.sll1572.

    Structurei

    Secondary structure

    1
    1355
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi781 – 7844
    Turni785 – 7873
    Beta strandi788 – 7914
    Helixi792 – 7976
    Beta strandi803 – 8075
    Beta strandi811 – 8177
    Beta strandi820 – 83415
    Beta strandi839 – 8424
    Beta strandi847 – 8504
    Beta strandi855 – 8573
    Helixi858 – 8647
    Beta strandi868 – 8714
    Beta strandi877 – 8793
    Turni883 – 8853
    Beta strandi887 – 8893
    Helixi890 – 8934
    Beta strandi896 – 8994
    Beta strandi901 – 91616
    Beta strandi923 – 9253
    Beta strandi928 – 9325

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1ZD7X-ray1.70A/B775-933[»]
    1ZDEX-ray1.95A770-936[»]
    3NZMX-ray1.55A772-933[»]
    4GIGX-ray1.80A775-933[»]
    ProteinModelPortaliP74750.
    SMRiP74750. Positions 775-933.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP74750.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0587.
    HOGENOMiHOG000021784.
    KOiK02337.
    OrthoDBiEOG6CZQGR.
    PhylomeDBiP74750.

    Family and domain databases

    Gene3Di2.170.16.10. 1 hit.
    2.40.50.140. 1 hit.
    InterProiIPR011708. DNA_pol3_alpha.
    IPR029460. DNAPol_HHH.
    IPR028992. Hedgehog/Intein_dom.
    IPR003587. Hint_dom_N.
    IPR006141. Intein_splice_site.
    IPR012340. NA-bd_OB-fold.
    IPR004365. NA-bd_OB_tRNA.
    IPR004013. PHP_C.
    IPR003141. Pol/His_phosphatase_N.
    IPR016195. Pol/histidinol_Pase-like.
    IPR004805. PolC_alpha.
    [Graphical view]
    PfamiPF07733. DNA_pol3_alpha. 1 hit.
    PF14579. HHH_6. 1 hit.
    PF02811. PHP. 1 hit.
    PF01336. tRNA_anti-codon. 1 hit.
    [Graphical view]
    SMARTiSM00306. HintN. 1 hit.
    SM00481. POLIIIAc. 1 hit.
    [Graphical view]
    SUPFAMiSSF51294. SSF51294. 1 hit.
    SSF89550. SSF89550. 1 hit.
    TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
    TIGR01445. intein_Nterm. 1 hit.
    TIGR00594. polc. 1 hit.
    PROSITEiPS50818. INTEIN_C_TER. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragments.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P74750-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSFVGLHIHS DYSLLDGASQ LPALIDRAIE LGMPAIALTD HGVMYGAVEL     50
    LKVCRGKPIK PIIGNEMYVI NGDIEINKRH RRFHQVVLAK NNQGYRNLVK 100
    LTTISNLKGI QGSGIFARPC INKELLEQYK EGLIVTSACL GGEVPQAILK 150
    GDLDHARQVA KWYKNLFGDD YYLEIQDHGS VEDRLVNINL VKIAQELDIK 200
    IVATNDSHFI SCNDVEAHDA LLCIQTGKLI TEEKRLRYSG TEYLKSAEEM 250
    HLLFRDHLPD DIIETAIANT LEVADKVEAY SILGEPRIPH YPIPPNHTPE 300
    TYVEDIAWDG LLERLKCRER ADITPVYKER LEYELRMLEK MGFCTYFLVV 350
    WDYIKYARDH GIPVGPGRGS AAGSLVAYCM KITNIDPVHH GLIFERFLNP 400
    ERKSMPDVDT DFCIDRRDEM IEYVTQKYGE DKVAQIITFN RMTSKAVLKD 450
    VARVLDIPYA ESDKMAKMIP VSRGKPAKLK VMISDQTPEP QFKARYDNEP 500
    WVKKWIDMAI RIEGTNKTFG VHAAGVVISS EPLDQIVPLQ KNNDGAVITQ 550
    YYMEDVEAMG LLKMDFLGLK NLTTLQRAVE LVAETKGIEL DLDDLPLQER 600
    KALQIRARTG SKKLPDDVKK THKLLEAGDL EGIFQLESQG MKQIVRDLKP 650
    SGIEDISSIL ALYRPGPLDA GLIPIFINRK HGREEISYQH KLLEPILNET 700
    YGVLVYQEQI MKMAQDLADY SLGEADLLRR AMGKKKAEEM QKHRAKFVDG 750
    STKHGVPSRI AENLFDQMVK FAEYCLSFGT EILTVEYGPL PIGKIVSEEI 800
    NCSVYSVDPE GRVYTQAIAQ WHDRGEQEVL EYELEDGSVI RATSDHRFLT 850
    TDYQLLAIEE IFARQLDLLT LENIKQTEEA LDNHRLPFPL LDAGTIKMVK 900
    VIGRRSLGVQ RIFDIGLPQD HNFLLANGAI AANCFNKSHS TAYAYVTYQT 950
    AYLKANYPVE YMAALLTASS DSQEKVEKYR ENCQKMGITV EPPDINRSQR 1000
    HFTPLGEAIL FGLSAVRNLG EGAIEQIITA RDNSEEKRFK SLADFCTQVD 1050
    LRVVNRRAIE TLIMAGAFDG VQSNRNQLLH DLELVIAWAQ KRAKEKETGQ 1100
    LNIFDSLTAG ESIKAKEAAN NGFEQEPSAP PVAEFSLQEK LQLEKEHLGF 1150
    YVSEHPLKSV QRSARLLSPI NLVDLDQYKV RQKVSAVAIL VAVKKIITKK 1200
    NGQPMAFLTL EDMSGQSEAV VFPSNYERLQ DVLIEGSQQM IWGKVDRRDD 1250
    QYQLIVEDLE PVEEVKMVML DLTPQEIANT STQARLKQIL QSHAPQKEQM 1300
    KIPVVACINE GPTKHFIRFG ENYWVNDYGT AVASLQELGF RARLEPLLPQ 1350
    SQSVG 1355
    Length:1,355
    Mass (Da):153,001
    Last modified:September 26, 2001 - v2
    Checksum:i7C4F61EE4C36EE0F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-adjacent residuesi897 – 8982Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000022 Genomic DNA. Translation: BAA18870.1. Sequence problems.
    BA000022 Genomic DNA. Translation: BAA17242.1. Sequence problems.
    PIRiA59016.
    S75328.
    S76958.
    RefSeqiNP_440562.1. NC_000911.1.
    NP_443058.1. NC_000911.1.
    YP_005650620.1. NC_017277.1.
    YP_005653117.1. NC_017277.1.
    YP_007450445.1. NC_020286.1.
    YP_007452933.1. NC_020286.1.

    Genome annotation databases

    EnsemblBacteriaiBAA17242; BAA17242; BAA17242.
    BAA18870; BAA18870; BAA18870.
    GeneIDi951978.
    953863.
    KEGGisyn:sll1572.
    syn:slr0603.
    syy:SYNGTS_0667.
    syy:SYNGTS_3164.
    syz:MYO_132000.
    syz:MYO_16730.
    PATRICi23838310. VBISynSp132158_0722.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000022 Genomic DNA. Translation: BAA18870.1 . Sequence problems.
    BA000022 Genomic DNA. Translation: BAA17242.1 . Sequence problems.
    PIRi A59016.
    S75328.
    S76958.
    RefSeqi NP_440562.1. NC_000911.1.
    NP_443058.1. NC_000911.1.
    YP_005650620.1. NC_017277.1.
    YP_005653117.1. NC_017277.1.
    YP_007450445.1. NC_020286.1.
    YP_007452933.1. NC_020286.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1ZD7 X-ray 1.70 A/B 775-933 [» ]
    1ZDE X-ray 1.95 A 770-936 [» ]
    3NZM X-ray 1.55 A 772-933 [» ]
    4GIG X-ray 1.80 A 775-933 [» ]
    ProteinModelPortali P74750.
    SMRi P74750. Positions 775-933.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P74750. 2 interactions.
    STRINGi 1148.sll1572.

    Protein family/group databases

    MEROPSi N10.003.

    Proteomic databases

    PRIDEi P74750.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA17242 ; BAA17242 ; BAA17242 .
    BAA18870 ; BAA18870 ; BAA18870 .
    GeneIDi 951978.
    953863.
    KEGGi syn:sll1572.
    syn:slr0603.
    syy:SYNGTS_0667.
    syy:SYNGTS_3164.
    syz:MYO_132000.
    syz:MYO_16730.
    PATRICi 23838310. VBISynSp132158_0722.

    Phylogenomic databases

    eggNOGi COG0587.
    HOGENOMi HOG000021784.
    KOi K02337.
    OrthoDBi EOG6CZQGR.
    PhylomeDBi P74750.

    Miscellaneous databases

    EvolutionaryTracei P74750.

    Family and domain databases

    Gene3Di 2.170.16.10. 1 hit.
    2.40.50.140. 1 hit.
    InterProi IPR011708. DNA_pol3_alpha.
    IPR029460. DNAPol_HHH.
    IPR028992. Hedgehog/Intein_dom.
    IPR003587. Hint_dom_N.
    IPR006141. Intein_splice_site.
    IPR012340. NA-bd_OB-fold.
    IPR004365. NA-bd_OB_tRNA.
    IPR004013. PHP_C.
    IPR003141. Pol/His_phosphatase_N.
    IPR016195. Pol/histidinol_Pase-like.
    IPR004805. PolC_alpha.
    [Graphical view ]
    Pfami PF07733. DNA_pol3_alpha. 1 hit.
    PF14579. HHH_6. 1 hit.
    PF02811. PHP. 1 hit.
    PF01336. tRNA_anti-codon. 1 hit.
    [Graphical view ]
    SMARTi SM00306. HintN. 1 hit.
    SM00481. POLIIIAc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51294. SSF51294. 1 hit.
    SSF89550. SSF89550. 1 hit.
    TIGRFAMsi TIGR01443. intein_Cterm. 1 hit.
    TIGR01445. intein_Nterm. 1 hit.
    TIGR00594. polc. 1 hit.
    PROSITEi PS50818. INTEIN_C_TER. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
      Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
      , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PCC 6803 / Kazusa.
    2. "Protein trans-splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803."
      Wu H., Hu Z., Liu X.-Q.
      Proc. Natl. Acad. Sci. U.S.A. 95:9226-9231(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION OF SPLIT INTEIN.
    3. "Protein trans-splicing and cyclization by a naturally split intein from the dnaE gene of Synechocystis species PCC6803."
      Evans T.C. Jr., Martin D., Kolly R., Panne D., Sun L., Ghosh I., Chen L., Benner J., Liu X.-Q., Xu M.-Q.
      J. Biol. Chem. 275:9091-9094(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION OF SPLIT INTEIN.
    4. "Characterization of a naturally occurring trans-splicing intein from Synechocystis sp. PCC6803."
      Martin D.D., Xu M.-Q., Evans T.C. Jr.
      Biochemistry 40:1393-1402(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION OF SPLIT INTEIN.

    Entry informationi

    Entry nameiDPO3A_SYNY3
    AccessioniPrimary (citable) accession number: P74750
    Secondary accession number(s): P73215
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: September 26, 2001
    Last modified: October 1, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The intein is a split intein capable of protein trans-splicing. The N- and C-terminal halves of dnaE are encoded by two separate genes located 745 kb apart in the genome and on opposite DNA strands. The dnaE-N product consists of a N-extein sequence followed by a 123-aa intein sequence (Ssp dnaE1), whereas the dnaE-C product consists of a 36-aa intein sequence (Ssp dnaE2) followed by a C-extein sequence. The N- and C-extein sequences together reconstitute a complete DnaE sequence that is interrupted by the intein sequences inside the beta- and tau-binding domains.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Intein-containing proteins
      List of intein-containing protein entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families
    4. Synechocystis PCC 6803
      Synechocystis (strain PCC 6803): entries and gene names

    External Data

    Dasty 3