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P74750

- DPO3A_SYNY3

UniProt

P74750 - DPO3A_SYNY3

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Protein

DNA polymerase III subunit alpha

Gene

dnaE-N

more
Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase (By similarity).By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

  1. 3'-5' exonuclease activity Source: InterPro
  2. DNA binding Source: InterPro
  3. DNA-directed DNA polymerase activity Source: UniProtKB-KW

GO - Biological processi

  1. intein-mediated protein splicing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Protein family/group databases

MEROPSiN10.003.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase III subunit alpha (EC:2.7.7.7)
Cleaved into the following chain:
Gene namesi
Name:dnaE-N
Ordered Locus Names:slr0603
AND
Name:dnaE-C
Ordered Locus Names:sll1572
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 774774DNA polymerase III subunit alpha, 1st partPRO_0000007364Add
BLAST
Chaini775 – 897123Ssp dnaE intein, 1st partPRO_0000007365Add
BLAST
Chaini898 – 93336Ssp dnaE intein, 2nd partPRO_0000007366Add
BLAST
Chaini934 – 1355422DNA polymerase III subunit alpha, 2nd partPRO_0000007367Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Proteomic databases

PRIDEiP74750.

Interactioni

Subunit structurei

DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the PolIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex (By similarity).By similarity

Protein-protein interaction databases

IntActiP74750. 2 interactions.
STRINGi1148.sll1572.

Structurei

Secondary structure

1
1355
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi781 – 7844Combined sources
Turni785 – 7873Combined sources
Beta strandi788 – 7914Combined sources
Helixi792 – 7976Combined sources
Beta strandi803 – 8075Combined sources
Beta strandi811 – 8177Combined sources
Beta strandi820 – 83415Combined sources
Beta strandi839 – 8424Combined sources
Beta strandi847 – 8504Combined sources
Beta strandi855 – 8573Combined sources
Helixi858 – 8647Combined sources
Beta strandi868 – 8714Combined sources
Beta strandi877 – 8793Combined sources
Turni883 – 8853Combined sources
Beta strandi887 – 8893Combined sources
Helixi890 – 8934Combined sources
Beta strandi896 – 8994Combined sources
Beta strandi901 – 91616Combined sources
Beta strandi923 – 9253Combined sources
Beta strandi928 – 9325Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZD7X-ray1.70A/B775-933[»]
1ZDEX-ray1.95A770-936[»]
3NZMX-ray1.55A772-933[»]
4GIGX-ray1.80A775-933[»]
ProteinModelPortaliP74750.
SMRiP74750. Positions 775-933.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP74750.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0587.
HOGENOMiHOG000021784.
InParanoidiP74750.
KOiK02337.
OrthoDBiEOG6CZQGR.
PhylomeDBiP74750.

Family and domain databases

Gene3Di2.170.16.10. 1 hit.
2.40.50.140. 1 hit.
InterProiIPR011708. DNA_pol3_alpha.
IPR029460. DNAPol_HHH.
IPR028992. Hedgehog/Intein_dom.
IPR003587. Hint_dom_N.
IPR006141. Intein_splice_site.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
IPR004013. PHP_C.
IPR003141. Pol/His_phosphatase_N.
IPR016195. Pol/histidinol_Pase-like.
IPR004805. PolC_alpha.
[Graphical view]
PfamiPF07733. DNA_pol3_alpha. 1 hit.
PF14579. HHH_6. 1 hit.
PF02811. PHP. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
SMARTiSM00306. HintN. 1 hit.
SM00481. POLIIIAc. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 1 hit.
SSF89550. SSF89550. 1 hit.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR00594. polc. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragments.

Sequence processingi: The displayed sequence is further processed into a mature form.

P74750-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSFVGLHIHS DYSLLDGASQ LPALIDRAIE LGMPAIALTD HGVMYGAVEL
60 70 80 90 100
LKVCRGKPIK PIIGNEMYVI NGDIEINKRH RRFHQVVLAK NNQGYRNLVK
110 120 130 140 150
LTTISNLKGI QGSGIFARPC INKELLEQYK EGLIVTSACL GGEVPQAILK
160 170 180 190 200
GDLDHARQVA KWYKNLFGDD YYLEIQDHGS VEDRLVNINL VKIAQELDIK
210 220 230 240 250
IVATNDSHFI SCNDVEAHDA LLCIQTGKLI TEEKRLRYSG TEYLKSAEEM
260 270 280 290 300
HLLFRDHLPD DIIETAIANT LEVADKVEAY SILGEPRIPH YPIPPNHTPE
310 320 330 340 350
TYVEDIAWDG LLERLKCRER ADITPVYKER LEYELRMLEK MGFCTYFLVV
360 370 380 390 400
WDYIKYARDH GIPVGPGRGS AAGSLVAYCM KITNIDPVHH GLIFERFLNP
410 420 430 440 450
ERKSMPDVDT DFCIDRRDEM IEYVTQKYGE DKVAQIITFN RMTSKAVLKD
460 470 480 490 500
VARVLDIPYA ESDKMAKMIP VSRGKPAKLK VMISDQTPEP QFKARYDNEP
510 520 530 540 550
WVKKWIDMAI RIEGTNKTFG VHAAGVVISS EPLDQIVPLQ KNNDGAVITQ
560 570 580 590 600
YYMEDVEAMG LLKMDFLGLK NLTTLQRAVE LVAETKGIEL DLDDLPLQER
610 620 630 640 650
KALQIRARTG SKKLPDDVKK THKLLEAGDL EGIFQLESQG MKQIVRDLKP
660 670 680 690 700
SGIEDISSIL ALYRPGPLDA GLIPIFINRK HGREEISYQH KLLEPILNET
710 720 730 740 750
YGVLVYQEQI MKMAQDLADY SLGEADLLRR AMGKKKAEEM QKHRAKFVDG
760 770 780 790 800
STKHGVPSRI AENLFDQMVK FAEYCLSFGT EILTVEYGPL PIGKIVSEEI
810 820 830 840 850
NCSVYSVDPE GRVYTQAIAQ WHDRGEQEVL EYELEDGSVI RATSDHRFLT
860 870 880 890 900
TDYQLLAIEE IFARQLDLLT LENIKQTEEA LDNHRLPFPL LDAGTIKMVK
910 920 930 940 950
VIGRRSLGVQ RIFDIGLPQD HNFLLANGAI AANCFNKSHS TAYAYVTYQT
960 970 980 990 1000
AYLKANYPVE YMAALLTASS DSQEKVEKYR ENCQKMGITV EPPDINRSQR
1010 1020 1030 1040 1050
HFTPLGEAIL FGLSAVRNLG EGAIEQIITA RDNSEEKRFK SLADFCTQVD
1060 1070 1080 1090 1100
LRVVNRRAIE TLIMAGAFDG VQSNRNQLLH DLELVIAWAQ KRAKEKETGQ
1110 1120 1130 1140 1150
LNIFDSLTAG ESIKAKEAAN NGFEQEPSAP PVAEFSLQEK LQLEKEHLGF
1160 1170 1180 1190 1200
YVSEHPLKSV QRSARLLSPI NLVDLDQYKV RQKVSAVAIL VAVKKIITKK
1210 1220 1230 1240 1250
NGQPMAFLTL EDMSGQSEAV VFPSNYERLQ DVLIEGSQQM IWGKVDRRDD
1260 1270 1280 1290 1300
QYQLIVEDLE PVEEVKMVML DLTPQEIANT STQARLKQIL QSHAPQKEQM
1310 1320 1330 1340 1350
KIPVVACINE GPTKHFIRFG ENYWVNDYGT AVASLQELGF RARLEPLLPQ

SQSVG
Length:1,355
Mass (Da):153,001
Last modified:September 26, 2001 - v2
Checksum:i7C4F61EE4C36EE0F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-adjacent residuesi897 – 8982Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA18870.1. Sequence problems.
BA000022 Genomic DNA. Translation: BAA17242.1. Sequence problems.
PIRiA59016.
S75328.
S76958.
RefSeqiNP_440562.1. NC_000911.1.
NP_443058.1. NC_000911.1.
YP_005650620.1. NC_017277.1.
YP_005653117.1. NC_017277.1.
YP_007450445.1. NC_020286.1.
YP_007452933.1. NC_020286.1.

Genome annotation databases

EnsemblBacteriaiBAA17242; BAA17242; BAA17242.
BAA18870; BAA18870; BAA18870.
GeneIDi951978.
953863.
KEGGisyn:sll1572.
syn:slr0603.
PATRICi23838310. VBISynSp132158_0722.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA18870.1 . Sequence problems.
BA000022 Genomic DNA. Translation: BAA17242.1 . Sequence problems.
PIRi A59016.
S75328.
S76958.
RefSeqi NP_440562.1. NC_000911.1.
NP_443058.1. NC_000911.1.
YP_005650620.1. NC_017277.1.
YP_005653117.1. NC_017277.1.
YP_007450445.1. NC_020286.1.
YP_007452933.1. NC_020286.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1ZD7 X-ray 1.70 A/B 775-933 [» ]
1ZDE X-ray 1.95 A 770-936 [» ]
3NZM X-ray 1.55 A 772-933 [» ]
4GIG X-ray 1.80 A 775-933 [» ]
ProteinModelPortali P74750.
SMRi P74750. Positions 775-933.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P74750. 2 interactions.
STRINGi 1148.sll1572.

Protein family/group databases

MEROPSi N10.003.

Proteomic databases

PRIDEi P74750.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA17242 ; BAA17242 ; BAA17242 .
BAA18870 ; BAA18870 ; BAA18870 .
GeneIDi 951978.
953863.
KEGGi syn:sll1572.
syn:slr0603.
PATRICi 23838310. VBISynSp132158_0722.

Phylogenomic databases

eggNOGi COG0587.
HOGENOMi HOG000021784.
InParanoidi P74750.
KOi K02337.
OrthoDBi EOG6CZQGR.
PhylomeDBi P74750.

Miscellaneous databases

EvolutionaryTracei P74750.

Family and domain databases

Gene3Di 2.170.16.10. 1 hit.
2.40.50.140. 1 hit.
InterProi IPR011708. DNA_pol3_alpha.
IPR029460. DNAPol_HHH.
IPR028992. Hedgehog/Intein_dom.
IPR003587. Hint_dom_N.
IPR006141. Intein_splice_site.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
IPR004013. PHP_C.
IPR003141. Pol/His_phosphatase_N.
IPR016195. Pol/histidinol_Pase-like.
IPR004805. PolC_alpha.
[Graphical view ]
Pfami PF07733. DNA_pol3_alpha. 1 hit.
PF14579. HHH_6. 1 hit.
PF02811. PHP. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view ]
SMARTi SM00306. HintN. 1 hit.
SM00481. POLIIIAc. 1 hit.
[Graphical view ]
SUPFAMi SSF51294. SSF51294. 1 hit.
SSF89550. SSF89550. 1 hit.
TIGRFAMsi TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR00594. polc. 1 hit.
PROSITEi PS50818. INTEIN_C_TER. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.
  2. "Protein trans-splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803."
    Wu H., Hu Z., Liu X.-Q.
    Proc. Natl. Acad. Sci. U.S.A. 95:9226-9231(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION OF SPLIT INTEIN.
  3. "Protein trans-splicing and cyclization by a naturally split intein from the dnaE gene of Synechocystis species PCC6803."
    Evans T.C. Jr., Martin D., Kolly R., Panne D., Sun L., Ghosh I., Chen L., Benner J., Liu X.-Q., Xu M.-Q.
    J. Biol. Chem. 275:9091-9094(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION OF SPLIT INTEIN.
  4. "Characterization of a naturally occurring trans-splicing intein from Synechocystis sp. PCC6803."
    Martin D.D., Xu M.-Q., Evans T.C. Jr.
    Biochemistry 40:1393-1402(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION OF SPLIT INTEIN.

Entry informationi

Entry nameiDPO3A_SYNY3
AccessioniPrimary (citable) accession number: P74750
Secondary accession number(s): P73215
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: September 26, 2001
Last modified: November 26, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The intein is a split intein capable of protein trans-splicing. The N- and C-terminal halves of dnaE are encoded by two separate genes located 745 kb apart in the genome and on opposite DNA strands. The dnaE-N product consists of a N-extein sequence followed by a 123-aa intein sequence (Ssp dnaE1), whereas the dnaE-C product consists of a 36-aa intein sequence (Ssp dnaE2) followed by a C-extein sequence. The N- and C-extein sequences together reconstitute a complete DnaE sequence that is interrupted by the intein sequences inside the beta- and tau-binding domains.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3