P74724 (PURK_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N5-carboxyaminoimidazole ribonucleotide synthase
Short name=N5-CAIR synthase
EC=6.3.4.18

Alternative name(s):
5-(carboxyamino)imidazole ribonucleotide synthetase

Gene names

Name:	purK
Ordered Locus Names:	sll0578

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa)

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO3- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) By similarity.
Catalytic activity	ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO3- = ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 1/2.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the purK/purT family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	5-(carboxyamino)imidazole ribonucleotide synthase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: InterPro phosphoribosylaminoimidazole carboxylase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	N5-carboxyaminoimidazole ribonucleotide synthase		PRO_0000075014
Regions
	Domain	109 – 297	189	ATP-grasp
	Nucleotide binding	174 – 177	4	ATP By similarity
	Nucleotide binding	267 – 268	2	ATP By similarity
Sites
	Binding site	105	1	ATP By similarity
	Binding site	140	1	ATP By similarity
	Binding site	182	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P74724 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: BE43B86DE7D32174	FASTA	388	42,642
	10 20 30 40 50 60 METKLTTVGV IGGGQLAWMM AQEAPRLGLK LAVQTPGESD PAVALADKVV LTAIADGEGT 70 80 90 100 110 120 RELAEHCGVI TFENEFVDLP SLEKLAQQGI TFHPRLDALA PLLDKWEQRH FLQNLGLPVP 130 140 150 160 170 180 DFWALESLPA QLPDFPWVLK ARRHGYDGQG TQIINSPADL PDLSKAPPGS WMVESFVPYE 190 200 210 220 230 240 RELAVIGVRN ARGEMEIYPV VETKQIDQVC RWVVAPAPID AAIAATAQDY CRRILNHLDY 250 260 270 280 290 300 VGILAIEFFL LPASTQTPTP EQRLLVNELA PRTHNSGHFS LDACHTSQFA LQLQAVTGQT 310 320 330 340 350 360 LGSSALRCGQ AVMVNLLGYE VSTGDYQQQL DKLAALPHSH VHWYGKGDCR PGRKLGHVTL 370 380 LNPDVESEPS TAWAEDLVKQ VEAIWYPD

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA18844.1.
PIR	S76932.
RefSeq	NP_443032.1. NC_000911.1.
3D structure databases
ProteinModelPortal	P74724.
ModBase	Search...
Protein-protein interaction databases
STRING	P74724.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	952576.
GenomeReviews	Gene locus sll0578 in contig BA000022_GR.
KEGG	syn:sll0578.
NMPDR	fig|1148.1.peg.3133.
PATRIC	23843920. VBISynSp132158_3490.
Phylogenomic databases
eggNOG	COG0026.
HOGENOM	HBG516369.
OMA	ICPARID.
PhylomeDB	P74724.
ProtClustDB	PRK06019.
Enzyme and pathway databases
BioCyc	SSP1148:SLL0578-MONOMER.
Family and domain databases
InterPro	IPR005875. AIR_COase_ATPase-su. IPR011761. ATP-grasp. IPR003135. ATP-grasp_carboxylate-amine. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR011054. Rudment_hybrid_motif. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
KO	K01589.
Pfam	PF02222. ATP-grasp. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit. SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMs	TIGR01161. PurK. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURK_SYNY3

Accession

Primary (citable) accession number: P74724

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program