Reviewed, UniProtKB/Swiss-Prot P74714 (UCRIA_SYNY3)

Names and origin

Protein names

Recommended name:
    Cytochrome b6-f complex iron-sulfur subunit 1
    EC=1.10.99.1
Alternative name(s):
    Rieske iron-sulfur protein 1
    Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein 1
      Short name=ISP 1
      Short name=RISP 1

Gene names

Name:	petC1
Ordered Locus Names:	slr1185

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	178 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions By similarity.
Catalytic activity	Plastoquinol-1 + 2 oxidized plastocyanin = plastoquinone + 2 reduced plastocyanin.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity.
Subunit structure	The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and the Rieske protein, while the 4 small subunits are petG, petL, petM and petN. The complex functions as a dimer By similarity.
Subcellular location	Cellular thylakoid membrane; Single-pass membrane proteinBy similarity. Note= The transmembrane helix obliquely spans the membrane in one monomer, and its extrinsic C-terminal domain is part of the other monomer By similarity.
Miscellaneous	The Rieske iron-sulfur protein is a high potential 2Fe-2S protein.
Sequence similarities	Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Membrane Thylakoid
Domain	Transmembrane
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: InterPro electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW plastoquinol-plastocyanin reductase activity Inferred from electronic annotation. Source: HAMAP ubiquinol-cytochrome-c reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 178	178	Cytochrome b6-f complex iron-sulfur subunit 1		PRO_0000127784
Regions
	Transmembrane	17 – 36	20	Potential
	Domain	61 – 161	101	Rieske
Sites
	Metal binding	107	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	109	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	125	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	128	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Amino acid modifications
	Disulfide bond	112 ↔ 127		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P74714-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 6AFF9F4195A61956	FASTA	178	18,685
	10 20 30 40 50 60 MDNTQAIAPP SYSRRQLLNF LAGTTVAVTA SAGAYAMGKF FVPPAEKGGA GGGIIAKDVL 70 80 90 100 110 120 GNPIPASQIL AEAPGTRALV AGLAGDPTYL IVKEDGSLDS IGIVDSCTHL GCTFPWNGND 130 140 150 160 170 QEFQCPCHGS RYHPDGSVAR GPAPLPLKIV QVAVVDDQIF ISPWTDLDPR TGEKPWWV

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000022 Genomic DNA. Translation: BAA18833.1.
PIR	S76921.
RefSeq	NP_443021.1.
3D structure databases
HSSP	HSSP built from PDB template 1RFS based on UniProtKB P08980.
ModBase	Search...
Protein-protein interaction databases
IntAct	P74714.
Genome annotation databases
GeneID	951899.
GenomeReviews	Gene locus slr1185 in contig BA000022_GR.
KEGG	syn:slr1185.
NMPDR	fig|1148.1.peg.3122.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P74714.
Enzyme and pathway databases
BioCyc	SSP1148:SLR1185-MON.
Family and domain databases
HAMAP	MF_01335. [Tree]
InterPro	IPR014909. Cyt_b6-F_Fe-S_su. IPR014349. Rieske. IPR005805. Rieske_C. IPR005806. Rieske_reg. [Graphical view]
Gene3D	G3DSA:2.102.10.10. Rieske_reg. 1 hit.
PANTHER	PTHR10134. Rieske. 1 hit.
Pfam	PF08802. CytB6-F_Fe-S. 1 hit. PF00355. Rieske. 1 hit. [Graphical view]
PRINTS	PR00162. RIESKE.
PROSITE	PS51296. RIESKE. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UCRIA_SYNY3

Accession

Primary (citable) accession number: P74714

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	February 1, 1997
Last modified:	November 25, 2008
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)