P74707 (RF1_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptide chain release factor 1
Short name=RF-1

Gene names

Name:	prfA
Ordered Locus Names:	sll1110

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechocystis › Synechocystis sp. PCC 6803

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA By similarity. HAMAP-Rule MF_00093
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00093.
Post-translational modification	Methylated by PrmC. Methylation increases the termination efficiency of RF1 By similarity. HAMAP-Rule MF_00093
Sequence similarities	Belongs to the prokaryotic/mitochondrial release factor family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
PTM	Methylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	translation release factor activity, codon specific Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 365	365	Peptide chain release factor 1 HAMAP-Rule MF_00093		PRO_0000177760
Amino acid modifications
	Modified residue	239	1	N5-methylglutamine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P74707 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: D565402A35E27EAC	FASTA	365	41,126
	10 20 30 40 50 60 MAETYLLDKL ASVEQTYQEL TRMLADPDIA TNPDELQRVA KARSSLEETV DTYETWKRSQ 70 80 90 100 110 120 EDLKGARQIV KESGNDPEMR EMAQLEVEEL ENAIGELEKR LTILLLPKDP NDEKNIMLEI 130 140 150 160 170 180 RAGTGGDEAS IWAGDLVRMY TRYAESQGWK VSLLSESLAD MGGFKEAILE VKGDRVYSQL 190 200 210 220 230 240 KFEAGVHRVQ RVPVTEAGGR VHTSTATVAI MPEVDDVEVK IDPKDIEMST ARSGGAGGQN 250 260 270 280 290 300 VNKVETAVDL FHKPTGIRIF CTEERSQLQN RERAMQILRA KLYDMMLQEQ NDAISSNRRS 310 320 330 340 350 360 QVGTGSRSEK IRTYNYKDNR VTDHRLGRNF DLNTALEGEI HTIIESCISQ DQQERLAELA EATNN

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA18826.1.
PIR	S76914.
RefSeq	NP_443014.1. NC_000911.1. YP_005653073.1. NC_017277.1. YP_007452889.1. NC_020286.1.
3D structure databases
ProteinModelPortal	P74707.
ModBase	Search...
Protein-protein interaction databases
IntAct	P74707. 2 interactions.
STRING	1148.sll1110.
Proteomic databases
PaxDb	P74707.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAA18826; BAA18826; BAA18826.
GeneID	12255604. 14618581. 951907.
KEGG	syn:sll1110. syy:SYNGTS_3120.
PATRIC	23843878. VBISynSp132158_3469.
Phylogenomic databases
eggNOG	COG0216.
HOGENOM	HOG000074815.
KO	K02835.
OMA	SVMLEIR.
ProtClustDB	PRK00591.
Family and domain databases
Gene3D	3.30.160.20. 1 hit.
HAMAP	MF_00093. Rel_fac_1.
InterPro	IPR014720. dsRNA-bd-like_dom. IPR005139. PCRF. IPR000352. Pep_chain_release_fac_I_II. IPR004373. PrfA. [Graphical view]
Pfam	PF03462. PCRF. 1 hit. PF00472. RF-1. 1 hit. [Graphical view]
SMART	SM00937. PCRF. 1 hit. [Graphical view]
TIGRFAMs	TIGR00019. prfA. 1 hit.
PROSITE	PS00745. RF_PROK_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RF1_SYNY3

Accession

Primary (citable) accession number: P74707

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program