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Protein

Carbamoyl-phosphate synthase small chain

Gene

carA

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes carbamoyl phosphate from bicarbonate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes carbamoyl phosphate from bicarbonate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB)
  2. Aspartate carbamoyltransferase (pyrB)
  3. Dihydroorotase (pyrC)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei272NucleophileUniRule annotation1
Active sitei354UniRule annotation1
Active sitei356UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Arginine biosynthesis, Pyrimidine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00171.
UPA00070; UER00115.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase small chainUniRule annotation (EC:6.3.5.5UniRule annotation)
Alternative name(s):
Carbamoyl-phosphate synthetase glutamine chainUniRule annotation
Gene namesi
Name:carAUniRule annotation
Ordered Locus Names:sll1498
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesMerismopediaceaeSynechocystis
Proteomesi
  • UP000001425 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001123391 – 384Carbamoyl-phosphate synthase small chainAdd BLAST384

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.UniRule annotation

Protein-protein interaction databases

IntActiP74587. 1 interactor.
STRINGi1148.SYNGTS_2991.

Structurei

3D structure databases

ProteinModelPortaliP74587.
SMRiP74587.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini196 – 381Glutamine amidotransferase type-1UniRule annotationAdd BLAST186

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 192CPSaseAdd BLAST192

Sequence similaritiesi

Belongs to the CarA family.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

HOGENOMiHOG000038087.
InParanoidiP74587.
KOiK01956.
OMAiCFNTGMT.
PhylomeDBiP74587.

Family and domain databases

CDDicd01744. GATase1_CPSase. 1 hit.
Gene3Di3.40.50.880. 1 hit.
3.50.30.20. 1 hit.
HAMAPiMF_01209. CPSase_S_chain. 1 hit.
InterProiView protein in InterPro
IPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR036480. CarbP_synth_ssu_N_sf.
IPR029062. Class_I_gatase-like.
IPR035686. CPSase_GATase1.
IPR017926. GATASE.
PfamiView protein in Pfam
PF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
SMARTiView protein in SMART
SM01097. CPSase_sm_chain. 1 hit.
SUPFAMiSSF52021. SSF52021. 1 hit.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01368. CPSaseIIsmall. 1 hit.
PROSITEiView protein in PROSITE
PS51273. GATASE_TYPE_1. 1 hit.

Sequencei

Sequence statusi: Complete.

P74587-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPIAAAKPAL LVLADGTPYP GWSFGADGTT VGEVVFNTGM TGYQEVMTDP
60 70 80 90 100
SYCGQIVTFT YPELGNTGVN AEDEESIHPH VKGVVARNIT RRPSNWRSTQ
110 120 130 140 150
SLPDYLVEHK IIGIYGIDTR ALTRKLRSIG AMNGGISTEI LEPEALLHHI
160 170 180 190 200
QAAPSMAGLN LVKEVTTHEV YEWTDSTNDH WQFGPVAEQQ GQPPLTVVAL
210 220 230 240 250
DFGVKRNILR RLASYGCRVI VVPASTSAAE ILQYNPDGIF LSNGPGDPSA
260 270 280 290 300
VEEGIVTTKE LLAAKKPMFG ICMGHQVLGL SLGAETFKLK FGHRGLNQPC
310 320 330 340 350
GLEQQVEITS QNHGFAVTEG SLAEEVEITH FNLNDKTVAG LRHKELPFFS
360 370 380
VQYHPEASPG PHDADYLFEK FVKLMRQQKA EVAG
Length:384
Mass (Da):41,817
Last modified:July 11, 2002 - v2
Checksum:i3F3F4E33E38947D6
GO

Sequence cautioni

P74587: The sequence BAA18695 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA18695.1. Different initiation.
PIRiS76783.

Genome annotation databases

EnsemblBacteriaiBAA18695; BAA18695; BAA18695.
KEGGisyn:sll1498.

Similar proteinsi

Entry informationi

Entry nameiCARA_SYNY3
AccessioniPrimary (citable) accession number: P74587
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: July 11, 2002
Last modified: October 25, 2017
This is version 124 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names