Reviewed, UniProtKB/Swiss-Prot P74582 (ACON2_SYNY3)

Names and origin

Protein names

Recommended name:
    Aconitate hydratase 2
      Short name=Aconitase 2
    EC=4.2.1.3
Alternative name(s):
    Citrate hydro-lyase 2

Gene names

Name:	acnB
Ordered Locus Names:	slr0665

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	868 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Citrate = isocitrate.
Cofactor	Binds 1 4Fe-4S cluster per subunit By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle.
Sequence similarities	Belongs to the aconitase/IPM isomerase family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW aconitate hydratase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 868	868	Aconitate hydratase 2		PRO_0000076678
Sites
	Metal binding	700	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	758	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	761	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P74582-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: EBF93B0F966A94EE	FASTA	868	93,552
	10 20 30 40 50 60 MLQAYRRHVA DRQKLGIPPL PLNAQQTTEL CELLKNPPEA EKEELMMLLR DRVPPGVDEA 70 80 90 100 110 120 AYVKAGFLTA IAKGEVTCPL ISGQGAVDLL GTMIGGYNVQ SLIELLKSKD TNIASAAATA 130 140 150 160 170 180 LSKTLLVFDA FNDVLHLSDT NGYAKQVIDA WAEGAWFINK PEVPERITVT VFKVPGETNT 190 200 210 220 230 240 DDLSPAPHAT TRPDIPLHAL AMLEAKMPEG LGTIAELKQK GHPVAYVGDV VGTGSSRKSA 250 260 270 280 290 300 INSVLWHIGT DIPFVPNKRA GGYILGGKIA PIFFNTAEDS GALPIECDVN QLNTGDVITI 310 320 330 340 350 360 YPREGKITNK AGETITTFQL KPETILDEVR AGGRIPLLIG RALTDKTREA LGLSPSPLFV 370 380 390 400 410 420 RPTAPADTGK GFTLAQKMVG KACGVQGIRP GTSCEPIMTT VGSQDTTGPM TRDELKELAC 430 440 450 460 470 480 LGFNADLTLQ TFCHTAAYPK PVDIKTHKDL PDFFSTRGGV ALRPGDGIIH SWLNRMLLPD 490 500 510 520 530 540 TVGTGGDSHT RFPLGISFPA GSGLVAFAAA LGVMPLDMPE SVLVKFTGEL QPGVTLRDIV 550 560 570 580 590 600 NAIPWVAMQQ GKLTVGKGDK VNVFNGRIME MEGLPDLKVE QAFELTDATA ERSCSGSTIK 610 620 630 640 650 660 LSEETVAEYL RSNVVLMKNM IARGYQDART LLRRIAKMEE WLANPSLMSG DADAEYADVI 670 680 690 700 710 720 EVNLDEIKEP IVAAPNDPDN VKLMSECAGD VIHEVFIGSC MTNIGHYRAA AKILEGAGTV 730 740 750 760 770 780 KGRLWICPPT RMDEQQLREE GIYGIFAAAG ARTEMPGCSL CMGNQARVED GVTVFSTSTR 790 800 810 820 830 840 NFNNRMGKGA QVYLGSAELA AVCALLGKIP TVEEYLAIVS EKVAPFEGEL YRYLNFNEID 850 860 NFEDFGRVIP LDQMPKIEDI LGMPVGAK

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000022 Genomic DNA. Translation: BAA18689.1.
PIR	S76777.
RefSeq	NP_442877.1.
3D structure databases
HSSP	HSSP built from PDB template 1L5J based on UniProtKB P36683.
ModBase	Search...
Protein-protein interaction databases
IntAct	P74582. 4 interactions.
STRING	P74582.
Genome annotation databases
GeneID	952027.
GenomeReviews	Gene locus slr0665 in contig BA000022_GR.
KEGG	syn:slr0665.
NMPDR	fig|1148.1.peg.2978.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P74582.
OMA	VELLGTM.
Enzyme and pathway databases
BioCyc	SSP1148:SLR0665-MON.
Family and domain databases
InterPro	IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3. IPR001030. Acoase/IPM_deHydtase_lsu_aba. IPR015937. Aconitase-like_core. IPR015928. Aconitase/3IPM_dehydase_swvl. IPR015932. Aconitase/IPMdHydase_lsu_aba_2. IPR018136. Aconitase_4Fe-4S_BS. IPR004406. Aconitase_B_bac. IPR015930. Aconitase_B_FeS-bd_bac. IPR015933. Aconitase_B_HEAT-like_bac. IPR015929. Aconitase_B_N_bac. [Graphical view]
Gene3D	G3DSA:3.30.499.10. Acnase/IPM_dHydase_lsu_aba_1/3. 2 hits. G3DSA:3.20.19.10. Aconitase/3IPM_dehydase_swvl. 1 hit. G3DSA:3.40.1060.10. Aconitase/IPMdHydase_lsu_aba_2. 1 hit. G3DSA:1.25.40.160. Aconitase_B_HEAT-like_bac. 1 hit.
PANTHER	PTHR11670. Aconitase-like_core. 1 hit. PTHR11670:SF3. Aconitase_2. 1 hit.
Pfam	PF00330. Aconitase. 1 hit. PF06434. Aconitase_2_N. 1 hit. [Graphical view]
PIRSF	PIRSF036687. AcnB. 1 hit.
ProDom	PD000511. Aconitase_N. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00117. acnB. 1 hit.
PROSITE	PS00450. ACONITASE_1. 1 hit. PS01244. ACONITASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACON2_SYNY3

Accession

Primary (citable) accession number: P74582

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 1, 1997
Last modified:	November 3, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)