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P74565 (SIGA_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RNA polymerase sigma factor SigA
Gene names
Name:sigA
Synonyms:rpoD, rpoD1
Ordered Locus Names:slr0653
OrganismSynechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]
Taxonomic identifier1111708 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis

Protein attributes

Sequence length425 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth By similarity. HAMAP-Rule MF_00963

Subunit structure

Interacts transiently with the RNA polymerase catalytic core By similarity.

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00963.

Sequence similarities

Belongs to the sigma-70 factor family. RpoD/SigA subfamily.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentCytoplasm
   LigandDNA-binding
   Molecular functionSigma factor
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtranscription initiation from bacterial-type RNA polymerase promoter

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: InterPro

sigma factor activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 425425RNA polymerase sigma factor SigA HAMAP-Rule MF_00963
PRO_0000093928

Regions

DNA binding386 – 40520H-T-H motif By similarity
Region193 – 26371Sigma-70 factor domain-2 By similarity
Region272 – 34776Sigma-70 factor domain-3 By similarity
Region360 – 41354Sigma-70 factor domain-4 By similarity
Motif217 – 2204Interaction with polymerase core subunit RpoC HAMAP-Rule MF_00963

Sequences

Sequence LengthMass (Da)Tools
P74565 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 4043FEA859B442D7

FASTA42549,936
        10         20         30         40         50         60 
MTQTKEPLTK AESAELEQEI ELSQYINTDD IDDDDIDVED LEQEVAATEG KEKKVRKIRK 

        70         80         90        100        110        120 
DAVKKKPYTE DSIRIYLQEI GRIRLLRAEE EIELARQIAD LLELELIRDN LTLQLERQPS 

       130        140        150        160        170        180 
ELEWGKQVWK LETAKQRLVG DKKKEPKKKD IDSYLANPDN ELSLENEWSQ QPNKNFAAFR 

       190        200        210        220        230        240 
RRLFLDRRAK DKMVQSNLRL VVSIAKKYMN RGLSFQDLIQ EGSLGLIRAA EKFDHEKGYK 

       250        260        270        280        290        300 
FSTYATWWIR QAITRAIADQ SRTIRLPVHL YETISRIKKT TKLLSQEMRR KPTEEEIAEK 

       310        320        330        340        350        360 
MEMTIEKLRF IAKSAQLPIS LETPIGKEED SRLGDFIEAD GETPEDEVSK NLLREDLENV 

       370        380        390        400        410        420 
LDTLSPRERD VLRLRYGLDD GRMKTLEEIG QIFNVTRERI RQIEAKALRK LRHPNRNSIL 


KEYIR 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. expand/collapse author list , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 6803 / Kazusa.
[2]"A new set of PCR primers for specific detection of the gene encoding the principal sigma factor in cyanobacteria."
Asayama M., Yamada A., Tanaka K., Takahashi H., Shirai M.
J. Gen. Appl. Microbiol. 42:511-515(1996)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 211-396.
Strain: 559.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000022 Genomic DNA. Translation: BAA18672.1.
D86213 Genomic DNA. Translation: BAA13043.1.
PIRS76760.
RefSeqNP_442860.1. NC_000911.1.
YP_005652921.1. NC_017277.1.
YP_007452736.1. NC_020286.1.

3D structure databases

ProteinModelPortalP74565.
SMRP74565. Positions 187-260, 347-424.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP74565. 42 interactions.
STRING1148.slr0653.

Proteomic databases

PaxDbP74565.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA18672; BAA18672; BAA18672.
GeneID12255281.
14618421.
953190.
KEGGsyn:slr0653.
syy:SYNGTS_2968.
syz:MYO_129970.
PATRIC23843506. VBISynSp132158_3288.

Phylogenomic databases

eggNOGCOG0568.
HOGENOMHOG000270273.
KOK03086.
OMAKTELMAP.
OrthoDBEOG6XHC70.
PhylomeDBP74565.
ProtClustDBPRK07406.

Family and domain databases

Gene3D1.10.10.10. 2 hits.
HAMAPMF_00963. Sigma70_RpoD_SigA.
InterProIPR014284. RNA_pol_sigma-70_dom.
IPR000943. RNA_pol_sigma70.
IPR009042. RNA_pol_sigma70_r1_2.
IPR007627. RNA_pol_sigma70_r2.
IPR007624. RNA_pol_sigma70_r3.
IPR007630. RNA_pol_sigma70_r4.
IPR013325. RNA_pol_sigma_r2.
IPR013324. RNA_pol_sigma_r3_r4.
IPR012760. RNA_pol_sigma_RpoD_C.
IPR017848. RNA_pol_sigma_RpoD_cyanobac.
IPR028630. Sigma70_RpoD.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF00140. Sigma70_r1_2. 1 hit.
PF04542. Sigma70_r2. 1 hit.
PF04539. Sigma70_r3. 1 hit.
PF04545. Sigma70_r4. 1 hit.
[Graphical view]
PRINTSPR00046. SIGMA70FCT.
SUPFAMSSF88659. SSF88659. 2 hits.
SSF88946. SSF88946. 2 hits.
TIGRFAMsTIGR02393. RpoD_Cterm. 1 hit.
TIGR02997. Sig70-cyanoRpoD. 1 hit.
TIGR02937. sigma70-ECF. 1 hit.
PROSITEPS00715. SIGMA70_1. 1 hit.
PS00716. SIGMA70_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSIGA_SYNY3
AccessionPrimary (citable) accession number: P74565
Secondary accession number(s): Q55351
Entry history
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: February 1, 1997
Last modified: April 16, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Synechocystis PCC 6803

Synechocystis (strain PCC 6803): entries and gene names

SIMILARITY comments

Index of protein domains and families