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P74565

- SIGA_SYNY3

UniProt

P74565 - SIGA_SYNY3

Protein

RNA polymerase sigma factor SigA

Gene

sigA

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth.UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi386 – 40520H-T-H motifUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-HAMAP
    2. sequence-specific DNA binding transcription factor activity Source: InterPro
    3. sigma factor activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. transcription initiation from bacterial-type RNA polymerase promoter Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Sigma factor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA polymerase sigma factor SigAUniRule annotation
    Gene namesi
    Name:sigAUniRule annotation
    Synonyms:rpoD, rpoD1
    Ordered Locus Names:slr0653
    OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
    Taxonomic identifieri1111708 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
    ProteomesiUP000001425: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 425425RNA polymerase sigma factor SigAPRO_0000093928Add
    BLAST

    Proteomic databases

    PaxDbiP74565.

    Interactioni

    Subunit structurei

    Interacts transiently with the RNA polymerase catalytic core.UniRule annotation

    Protein-protein interaction databases

    IntActiP74565. 42 interactions.
    STRINGi1148.slr0653.

    Structurei

    3D structure databases

    ProteinModelPortaliP74565.
    SMRiP74565. Positions 187-260, 347-424.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni193 – 26371Sigma-70 factor domain-2UniRule annotationAdd
    BLAST
    Regioni272 – 34776Sigma-70 factor domain-3UniRule annotationAdd
    BLAST
    Regioni360 – 41354Sigma-70 factor domain-4UniRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi217 – 2204Interaction with polymerase core subunit RpoC

    Sequence similaritiesi

    Belongs to the sigma-70 factor family. RpoD/SigA subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0568.
    HOGENOMiHOG000270273.
    KOiK03086.
    OMAiIVEDGEM.
    OrthoDBiEOG6XHC70.
    PhylomeDBiP74565.

    Family and domain databases

    Gene3Di1.10.10.10. 2 hits.
    HAMAPiMF_00963. Sigma70_RpoD_SigA.
    InterProiIPR014284. RNA_pol_sigma-70_dom.
    IPR000943. RNA_pol_sigma70.
    IPR009042. RNA_pol_sigma70_r1_2.
    IPR007627. RNA_pol_sigma70_r2.
    IPR007624. RNA_pol_sigma70_r3.
    IPR007630. RNA_pol_sigma70_r4.
    IPR013325. RNA_pol_sigma_r2.
    IPR013324. RNA_pol_sigma_r3_r4.
    IPR012760. RNA_pol_sigma_RpoD_C.
    IPR017848. RNA_pol_sigma_RpoD_cyanobac.
    IPR028630. Sigma70_RpoD.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00140. Sigma70_r1_2. 1 hit.
    PF04542. Sigma70_r2. 1 hit.
    PF04539. Sigma70_r3. 1 hit.
    PF04545. Sigma70_r4. 1 hit.
    [Graphical view]
    PRINTSiPR00046. SIGMA70FCT.
    SUPFAMiSSF88659. SSF88659. 2 hits.
    SSF88946. SSF88946. 2 hits.
    TIGRFAMsiTIGR02393. RpoD_Cterm. 1 hit.
    TIGR02997. Sig70-cyanoRpoD. 1 hit.
    TIGR02937. sigma70-ECF. 1 hit.
    PROSITEiPS00715. SIGMA70_1. 1 hit.
    PS00716. SIGMA70_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P74565-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTQTKEPLTK AESAELEQEI ELSQYINTDD IDDDDIDVED LEQEVAATEG    50
    KEKKVRKIRK DAVKKKPYTE DSIRIYLQEI GRIRLLRAEE EIELARQIAD 100
    LLELELIRDN LTLQLERQPS ELEWGKQVWK LETAKQRLVG DKKKEPKKKD 150
    IDSYLANPDN ELSLENEWSQ QPNKNFAAFR RRLFLDRRAK DKMVQSNLRL 200
    VVSIAKKYMN RGLSFQDLIQ EGSLGLIRAA EKFDHEKGYK FSTYATWWIR 250
    QAITRAIADQ SRTIRLPVHL YETISRIKKT TKLLSQEMRR KPTEEEIAEK 300
    MEMTIEKLRF IAKSAQLPIS LETPIGKEED SRLGDFIEAD GETPEDEVSK 350
    NLLREDLENV LDTLSPRERD VLRLRYGLDD GRMKTLEEIG QIFNVTRERI 400
    RQIEAKALRK LRHPNRNSIL KEYIR 425
    Length:425
    Mass (Da):49,936
    Last modified:February 1, 1997 - v1
    Checksum:i4043FEA859B442D7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000022 Genomic DNA. Translation: BAA18672.1.
    D86213 Genomic DNA. Translation: BAA13043.1.
    PIRiS76760.
    RefSeqiNP_442860.1. NC_000911.1.
    YP_005652921.1. NC_017277.1.
    YP_007452736.1. NC_020286.1.

    Genome annotation databases

    EnsemblBacteriaiBAA18672; BAA18672; BAA18672.
    GeneIDi953190.
    KEGGisyn:slr0653.
    syy:SYNGTS_2968.
    syz:MYO_129970.
    PATRICi23843506. VBISynSp132158_3288.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000022 Genomic DNA. Translation: BAA18672.1 .
    D86213 Genomic DNA. Translation: BAA13043.1 .
    PIRi S76760.
    RefSeqi NP_442860.1. NC_000911.1.
    YP_005652921.1. NC_017277.1.
    YP_007452736.1. NC_020286.1.

    3D structure databases

    ProteinModelPortali P74565.
    SMRi P74565. Positions 187-260, 347-424.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P74565. 42 interactions.
    STRINGi 1148.slr0653.

    Proteomic databases

    PaxDbi P74565.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA18672 ; BAA18672 ; BAA18672 .
    GeneIDi 953190.
    KEGGi syn:slr0653.
    syy:SYNGTS_2968.
    syz:MYO_129970.
    PATRICi 23843506. VBISynSp132158_3288.

    Phylogenomic databases

    eggNOGi COG0568.
    HOGENOMi HOG000270273.
    KOi K03086.
    OMAi IVEDGEM.
    OrthoDBi EOG6XHC70.
    PhylomeDBi P74565.

    Family and domain databases

    Gene3Di 1.10.10.10. 2 hits.
    HAMAPi MF_00963. Sigma70_RpoD_SigA.
    InterProi IPR014284. RNA_pol_sigma-70_dom.
    IPR000943. RNA_pol_sigma70.
    IPR009042. RNA_pol_sigma70_r1_2.
    IPR007627. RNA_pol_sigma70_r2.
    IPR007624. RNA_pol_sigma70_r3.
    IPR007630. RNA_pol_sigma70_r4.
    IPR013325. RNA_pol_sigma_r2.
    IPR013324. RNA_pol_sigma_r3_r4.
    IPR012760. RNA_pol_sigma_RpoD_C.
    IPR017848. RNA_pol_sigma_RpoD_cyanobac.
    IPR028630. Sigma70_RpoD.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00140. Sigma70_r1_2. 1 hit.
    PF04542. Sigma70_r2. 1 hit.
    PF04539. Sigma70_r3. 1 hit.
    PF04545. Sigma70_r4. 1 hit.
    [Graphical view ]
    PRINTSi PR00046. SIGMA70FCT.
    SUPFAMi SSF88659. SSF88659. 2 hits.
    SSF88946. SSF88946. 2 hits.
    TIGRFAMsi TIGR02393. RpoD_Cterm. 1 hit.
    TIGR02997. Sig70-cyanoRpoD. 1 hit.
    TIGR02937. sigma70-ECF. 1 hit.
    PROSITEi PS00715. SIGMA70_1. 1 hit.
    PS00716. SIGMA70_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
      Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
      , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PCC 6803 / Kazusa.
    2. "A new set of PCR primers for specific detection of the gene encoding the principal sigma factor in cyanobacteria."
      Asayama M., Yamada A., Tanaka K., Takahashi H., Shirai M.
      J. Gen. Appl. Microbiol. 42:511-515(1996)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 211-396.
      Strain: 559.

    Entry informationi

    Entry nameiSIGA_SYNY3
    AccessioniPrimary (citable) accession number: P74565
    Secondary accession number(s): Q55351
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 24, 2004
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Synechocystis PCC 6803
      Synechocystis (strain PCC 6803): entries and gene names

    External Data

    Dasty 3