Reviewed, UniProtKB/Swiss-Prot P74562 (NADB_SYNY3)

Names and origin

Protein names

Recommended name:
    L-aspartate oxidase
      Short name=LASPO
    EC=1.4.3.16
Alternative name(s):
    Quinolinate synthetase B

Gene names

Name:	nadB
Ordered Locus Names:	sll0631

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	553 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of L-aspartate to iminoaspartate.
Catalytic activity	L-aspartate + H(2)O + O(2) = oxaloacetate + NH(3) + H(2)O(2).
Cofactor	FAD.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (oxidase route): step 1/1.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	L-aspartate oxidase activity Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 553	553	L-aspartate oxidase		PRO_0000184402
Regions
	Nucleotide binding	9 – 23	15	FAD Potential
Sites
	Active site	229	1	By similarity
	Active site	248	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P74562-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 0ABCE7366B9940CC	FASTA	553	60,498
	10 20 30 40 50 60 MGANQFDVVV VGSGAAGLYA CLCLPGHYRV ALVTKAELKT GASDWAQGGI AAAIAPTDSP 70 80 90 100 110 120 QSHYEDTLAA GAGLCDGEAV DFLVNHAPQA IAELVQFGVS FDRHGQHLAL TLEAAHSQPR 130 140 150 160 170 180 VLHAADTTGR AIVSTLMGQV QARPNVEIFS QAIALSLNIE PTTGHCRGIQ VFVNQTIETF 190 200 210 220 230 240 HSRAVLLATG GGGQVFAQTT NPKVSTGDGI ALAWRSGAQV RDLEFFQFHP TALTKPGVPH 250 260 270 280 290 300 FLISEAVRGE GAHLLDRQGK RFAFDYHPRG ELAPRDVVSR AIFQHLANTE KDPTQATVFL 310 320 330 340 350 360 DLSPIEPERI QRRFPNIIRR CLHWGVDIFR EPIPVAPAAH YWMGGITTDI NCQTTIPGLY 370 380 390 400 410 420 ALGETASTGV HGANRLASNS LLECIVFASQ LRNLSLPPLA SSDSNVNQSI KEIRLDTTND 430 440 450 460 470 480 LALLNHWRSE LPRLMWQTAG ICRQAETLQM AIAKLEQWQE QWQQLSSSKL LAHLPEDQKI 490 500 510 520 530 540 SLSGPGLNEF MQLWAETHNL LDIAQLILTS ALFREESRGG HYRLDCPDTK REWQSHTVIE 550 GTRVFLQPSQ SQD

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000022 Genomic DNA. Translation: BAA18669.1.
PIR	S76757.
RefSeq	NP_442857.1.
3D structure databases
HSSP	HSSP built from PDB template 1CHU based on UniProtKB P10902.
ModBase	Search...
Protein-protein interaction databases
IntAct	P74562.
Genome annotation databases
GeneID	952038.
GenomeReviews	Gene locus sll0631 in contig BA000022_GR.
KEGG	syn:sll0631.
NMPDR	fig|1148.1.peg.2958.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P74562.
Enzyme and pathway databases
BioCyc	SSP1148:SLL0631-MON.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR004112. Fum_Rdtase/Succ_DHase_flav_C. IPR005288. NadB. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00551. nadB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADB_SYNY3

Accession

Primary (citable) accession number: P74562

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	November 25, 2008
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)