P74467 (CLPP3_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable ATP-dependent Clp protease proteolytic subunit 3
EC=3.4.21.92

Alternative name(s):
Endopeptidase Clp 3

Gene names

Name:	clpP3
Ordered Locus Names:	slr0165

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechocystis › Synechocystis sp. PCC 6803

Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins By similarity. HAMAP-Rule MF_00444
Catalytic activity	Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs). HAMAP-Rule MF_00444
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase S14 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 202	202	Probable ATP-dependent Clp protease proteolytic subunit 3 HAMAP-Rule MF_00444		PRO_0000179696
Sites
	Active site	101	1	By similarity
	Active site	126	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P74467 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: AAC31B70F5ACBAC8	FASTA	202	22,397
	10 20 30 40 50 60 MPIGVPSVPF RLPGSQYERW IDIYTRLSQE RIIFLGQEVN DSIANRIVAF LLYLDSDDPS 70 80 90 100 110 120 KPIYLYINSP GGSVTAGMAI YDTMQYIKAE VITICVGLAA SMGAFLLASG APGKRLALPH 130 140 150 160 170 180 ARIMIHQPMG GTGRRQATDI DIEAREILRI RQQLNEIMAQ RTGQTVEKIA KDTDRDYFLS 190 200 AAEAKEYGLI DKVIENSTMG NN

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA18568.1.
PIR	S76439.
RefSeq	NP_441890.1. NC_000911.1. YP_005651949.1. NC_017277.1. YP_007451770.1. NC_020286.1.
3D structure databases
ProteinModelPortal	P74467.
SMR	P74467. Positions 15-194.
ModBase	Search...
Protein-protein interaction databases
IntAct	P74467. 3 interactions.
STRING	1148.slr0165.
Protein family/group databases
MEROPS	S14.001.
Proteomic databases
PaxDb	P74467.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAA18568; BAA18568; BAA18568.
GeneID	12254608. 14617437. 954955.
KEGG	syn:slr0165. syy:SYNGTS_1996.
PATRIC	23841315. VBISynSp132158_2207.
Phylogenomic databases
eggNOG	COG0740.
HOGENOM	HOG000285833.
KO	K01358.
OMA	NKPIYLY.
ProtClustDB	PRK00277.
Family and domain databases
HAMAP	MF_00444. ClpP.
InterPro	IPR001907. ClpP. IPR023562. ClpP/TepA. IPR018215. ClpP_AS. [Graphical view]
PANTHER	PTHR10381. PTHR10381. 1 hit.
Pfam	PF00574. CLP_protease. 1 hit. [Graphical view]
PRINTS	PR00127. CLPPROTEASEP.
PROSITE	PS00382. CLP_PROTEASE_HIS. 1 hit. PS00381. CLP_PROTEASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLPP3_SYNY3

Accession

Primary (citable) accession number: P74467

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program