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Protein

Chaperone protein ClpB 1

Gene

clpB1

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of ClpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by DnaK (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi208 – 215ATP 1By similarity8
Nucleotide bindingi620 – 627ATP 2By similarity8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein ClpB 1
Gene namesi
Name:clpB1
Ordered Locus Names:slr0156
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesMerismopediaceaeSynechocystis
Proteomesi
  • UP000001425 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001911921 – 898Chaperone protein ClpB 1Add BLAST898

Proteomic databases

PRIDEiP74459.

Interactioni

Subunit structurei

Homohexamer. The oligomerization is ATP-dependent (By similarity).By similarity

Protein-protein interaction databases

DIPiDIP-48813N.
IntActiP74459. 2 interactors.

Structurei

3D structure databases

ProteinModelPortaliP74459.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 145N-terminalBy similarityAdd BLAST145
Regioni161 – 344NBD1By similarityAdd BLAST184
Regioni345 – 560LinkerBy similarityAdd BLAST216
Regioni570 – 781NBD2By similarityAdd BLAST212
Regioni782 – 898C-terminalBy similarityAdd BLAST117

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili395 – 536By similarityAdd BLAST142

Domaini

The N-terminal domain probably functions as a substrate-discriminating domain, recruiting aggregated proteins to the ClpB hexamer and/or stabilizing bound proteins. The NBD2 domain is responsible for oligomerization, whereas the NBD1 domain stabilizes the hexamer probably in an ATP-dependent manner. The movement of the coiled-coil domain is essential for ClpB ability to rescue proteins from an aggregated state, probably by pulling apart large aggregated proteins, which are bound between the coiled-coils motifs of adjacent ClpB subunits in the functional hexamer (By similarity).By similarity

Sequence similaritiesi

Belongs to the ClpA/ClpB family.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

HOGENOMiHOG000218211.
InParanoidiP74459.
KOiK03695.
OMAiWHILLAM.
PhylomeDBiP74459.

Family and domain databases

Gene3Di1.10.1780.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR017730. Chaperonin_ClpB.
IPR019489. Clp_ATPase_C.
IPR004176. Clp_N.
IPR001270. ClpA/B.
IPR018368. ClpA/B_CS1.
IPR028299. ClpA/B_CS2.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF07724. AAA_2. 1 hit.
PF02861. Clp_N. 2 hits.
PF10431. ClpB_D2-small. 1 hit.
[Graphical view]
PRINTSiPR00300. CLPPROTEASEA.
SMARTiSM00382. AAA. 2 hits.
SM01086. ClpB_D2-small. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF81923. SSF81923. 1 hit.
TIGRFAMsiTIGR03346. chaperone_ClpB. 1 hit.
PROSITEiPS00870. CLPAB_1. 1 hit.
PS00871. CLPAB_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P74459-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQPTDPTKFT EQAWDAIVKS QEVARRYKNT NLEVEHILLA LLEQDMGLAA
60 70 80 90 100
RIFQRAAVDG EGLRQQLEIF TNRQPKQAYV EQLYLGRSLD VMLDRADAAR
110 120 130 140 150
SSWEDKFISV EHLLVGFAED DRVGRKTLRA FNLDPQDLEL AIKAIRGSQK
160 170 180 190 200
VTEPNQEEKY EALDKYGRDL TEQARQGKLD PVIGRDEEIR RVIQVLSRRS
210 220 230 240 250
KNNPVLIGEP GVGKTAIAEG LAQRIINGDV PESLKNRQLI SLDMGSLIAG
260 270 280 290 300
AKYRGEFEER LRSVMKEVTN SDGQIILFID EVHTVVGAGG REGSGSMDAG
310 320 330 340 350
NLLKPMLARG ELRCIGATTL DEYRKNIEKD PALERRFQQV YVKQPSVDDT
360 370 380 390 400
ISILRGLKEK YEVHHGVKIT DSALVAAATL SHRYIQDRFL PDKAIDLVDE
410 420 430 440 450
AAARLKMEIT SKPVELEDID RRLMQLQMEK LSLEGEEKRP GLGADKSSKE
460 470 480 490 500
RLEKIQQEIT ELEGQQQELS GQWLSEKQML EEINTLKEKE QELRLQVEKA
510 520 530 540 550
ERATDWEKAA KIKYGELEVL QHDIEEKESK LLEIQGSGNT LLREQVTESD
560 570 580 590 600
IAEIVAGWTG IPMNRLMETE RQKLLQLEGH LHQRVIGQKE AVAAVSAAIR
610 620 630 640 650
RARAGMKDPS RPIGSFLFMG PTGVGKTELA RALAGFLFDS EEAMVRIDMS
660 670 680 690 700
EYMEKHAVSR LIGAPPGYVG YEEGGQLSEA VRRRPYSVVL LDEVEKAHLD
710 720 730 740 750
VFNILLQVLD DGRITDSQGR VVDFRNTIIV MTSNIGSDHI LSLSADDADY
760 770 780 790 800
DKMQKQVLQS LRKHFRPEFL NRIDDLIIFH TLKRDELRRI VVLQIKRIEK
810 820 830 840 850
LLDEQKITLS LSDAALDHIV SAGYDPTYGA RPLKRAIQRQ LENPIATKIL
860 870 880 890
ENTFVAGDKI LIDCVDGQLI FDKEIEPTTL PEEPEENITA VEVEVLSS
Length:898
Mass (Da):101,392
Last modified:February 1, 1997 - v1
Checksum:i67BBA62136E36D63
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA18560.1.
PIRiS76431.

Genome annotation databases

EnsemblBacteriaiBAA18560; BAA18560; BAA18560.
KEGGisyn:slr0156.
PATRICi23841298. VBISynSp132158_2199.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA18560.1.
PIRiS76431.

3D structure databases

ProteinModelPortaliP74459.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48813N.
IntActiP74459. 2 interactors.

Proteomic databases

PRIDEiP74459.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA18560; BAA18560; BAA18560.
KEGGisyn:slr0156.
PATRICi23841298. VBISynSp132158_2199.

Phylogenomic databases

HOGENOMiHOG000218211.
InParanoidiP74459.
KOiK03695.
OMAiWHILLAM.
PhylomeDBiP74459.

Family and domain databases

Gene3Di1.10.1780.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR017730. Chaperonin_ClpB.
IPR019489. Clp_ATPase_C.
IPR004176. Clp_N.
IPR001270. ClpA/B.
IPR018368. ClpA/B_CS1.
IPR028299. ClpA/B_CS2.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF07724. AAA_2. 1 hit.
PF02861. Clp_N. 2 hits.
PF10431. ClpB_D2-small. 1 hit.
[Graphical view]
PRINTSiPR00300. CLPPROTEASEA.
SMARTiSM00382. AAA. 2 hits.
SM01086. ClpB_D2-small. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF81923. SSF81923. 1 hit.
TIGRFAMsiTIGR03346. chaperone_ClpB. 1 hit.
PROSITEiPS00870. CLPAB_1. 1 hit.
PS00871. CLPAB_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLPB1_SYNY3
AccessioniPrimary (citable) accession number: P74459
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.