P74459 (CLPB1_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chaperone protein ClpB 1

Gene names

Name:	clpB1
Ordered Locus Names:	slr0156

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechocystis › Synechocystis sp. PCC 6803

Protein attributes

Sequence length	898 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of ClpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by DnaK By similarity.
Subunit structure	Homohexamer. The oligomerization is ATP-dependent By similarity.
Subcellular location	Cytoplasm Probable.
Domain	The N-terminal domain probably functions as a substrate-discriminating domain, recruiting aggregated proteins to the ClpB hexamer and/or stabilizing bound proteins. The NBD2 domain is responsible for oligomerization, whereas the NBD1 domain stabilizes the hexamer probably in an ATP-dependent manner. The movement of the coiled-coil domain is essential for ClpB ability to rescue proteins from an aggregated state, probably by pulling apart large aggregated proteins, which are bound between the coiled-coils motifs of adjacent ClpB subunits in the functional hexamer By similarity.
Sequence similarities	Belongs to the clpA/clpB family.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Domain	Coiled coil Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein processing Inferred from electronic annotation. Source: InterPro response to heat Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 898	898	Chaperone protein ClpB 1		PRO_0000191192
Regions
	Nucleotide binding	208 – 215	8	ATP 1 By similarity
	Nucleotide binding	620 – 627	8	ATP 2 By similarity
	Region	1 – 145	145	N-terminal By similarity
	Region	161 – 344	184	NBD1 By similarity
	Region	345 – 560	216	Linker By similarity
	Region	570 – 781	212	NBD2 By similarity
	Region	782 – 898	117	C-terminal By similarity
	Coiled coil	395 – 536	142	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P74459 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 67BBA62136E36D63	FASTA	898	101,392
	10 20 30 40 50 60 MQPTDPTKFT EQAWDAIVKS QEVARRYKNT NLEVEHILLA LLEQDMGLAA RIFQRAAVDG 70 80 90 100 110 120 EGLRQQLEIF TNRQPKQAYV EQLYLGRSLD VMLDRADAAR SSWEDKFISV EHLLVGFAED 130 140 150 160 170 180 DRVGRKTLRA FNLDPQDLEL AIKAIRGSQK VTEPNQEEKY EALDKYGRDL TEQARQGKLD 190 200 210 220 230 240 PVIGRDEEIR RVIQVLSRRS KNNPVLIGEP GVGKTAIAEG LAQRIINGDV PESLKNRQLI 250 260 270 280 290 300 SLDMGSLIAG AKYRGEFEER LRSVMKEVTN SDGQIILFID EVHTVVGAGG REGSGSMDAG 310 320 330 340 350 360 NLLKPMLARG ELRCIGATTL DEYRKNIEKD PALERRFQQV YVKQPSVDDT ISILRGLKEK 370 380 390 400 410 420 YEVHHGVKIT DSALVAAATL SHRYIQDRFL PDKAIDLVDE AAARLKMEIT SKPVELEDID 430 440 450 460 470 480 RRLMQLQMEK LSLEGEEKRP GLGADKSSKE RLEKIQQEIT ELEGQQQELS GQWLSEKQML 490 500 510 520 530 540 EEINTLKEKE QELRLQVEKA ERATDWEKAA KIKYGELEVL QHDIEEKESK LLEIQGSGNT 550 560 570 580 590 600 LLREQVTESD IAEIVAGWTG IPMNRLMETE RQKLLQLEGH LHQRVIGQKE AVAAVSAAIR 610 620 630 640 650 660 RARAGMKDPS RPIGSFLFMG PTGVGKTELA RALAGFLFDS EEAMVRIDMS EYMEKHAVSR 670 680 690 700 710 720 LIGAPPGYVG YEEGGQLSEA VRRRPYSVVL LDEVEKAHLD VFNILLQVLD DGRITDSQGR 730 740 750 760 770 780 VVDFRNTIIV MTSNIGSDHI LSLSADDADY DKMQKQVLQS LRKHFRPEFL NRIDDLIIFH 790 800 810 820 830 840 TLKRDELRRI VVLQIKRIEK LLDEQKITLS LSDAALDHIV SAGYDPTYGA RPLKRAIQRQ 850 860 870 880 890 LENPIATKIL ENTFVAGDKI LIDCVDGQLI FDKEIEPTTL PEEPEENITA VEVEVLSS

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA18560.1.
PIR	S76431.
RefSeq	NP_441882.1. NC_000911.1. YP_005651942.1. NC_017277.1. YP_007451763.1. NC_020286.1.
3D structure databases
ProteinModelPortal	P74459.
SMR	P74459. Positions 161-355.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48813N.
IntAct	P74459. 2 interactions.
STRING	1148.slr0156.
Proteomic databases
PaxDb	P74459.
PRIDE	P74459.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAA18560; BAA18560; BAA18560.
GeneID	12254601. 14617430. 952862.
KEGG	syn:slr0156. syy:SYNGTS_1989.
PATRIC	23841298. VBISynSp132158_2199.
Phylogenomic databases
eggNOG	COG0542.
HOGENOM	HOG000218211.
KO	K03695.
OMA	SDQLYLG.
ProtClustDB	CLSK893322.
Family and domain databases
Gene3D	1.10.1780.10. 1 hit.
InterPro	IPR003593. AAA+_ATPase. IPR013093. ATPase_AAA-2. IPR003959. ATPase_AAA_core. IPR018368. Chaperonin_ClpA/B_CS. IPR017730. Chaperonin_ClpB. IPR001270. Chaprnin_ClpA/B. IPR019489. Clp_ATPase_C. IPR004176. Clp_N. IPR023150. Dbl_Clp-N. IPR027417. P-loop_NTPase. [Graphical view]
Pfam	PF00004. AAA. 1 hit. PF07724. AAA_2. 1 hit. PF02861. Clp_N. 2 hits. PF10431. ClpB_D2-small. 1 hit. [Graphical view]
PRINTS	PR00300. CLPPROTEASEA.
SMART	SM00382. AAA. 2 hits. SM01086. ClpB_D2-small. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 2 hits.
TIGRFAMs	TIGR03346. chaperone_ClpB. 1 hit.
PROSITE	PS00870. CLPAB_1. 1 hit. PS00871. CLPAB_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLPB1_SYNY3

Accession

Primary (citable) accession number: P74459

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	February 1, 1997
Last modified:	May 29, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program