Reviewed, UniProtKB/Swiss-Prot P74457 (PYRH_SYNY3)

Names and origin

Protein names

Recommended name:
    Uridylate kinase
      Short name=UK
    EC=2.7.4.22
Alternative name(s):
    Uridine monophosphate kinase
      Short name=UMP kinase
      Short name=UMPK

Gene names

Name:	pyrH
Ordered Locus Names:	sll0144

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible phosphorylation of UMP to UDP By similarity.
Catalytic activity	ATP + UMP = ADP + UDP. HAMAP MF_01220
Enzyme regulation	Allosterically activated by GTP. Inhibited by UTP By similarity.
Pathway	Pyrimidine metabolism; CTP biosynthesis via de novo pathway; UDP from UMP (UMPK route): step 1/1. HAMAP MF_01220
Subunit structure	Homohexamer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the UMP kinase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro cellular amino acid biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UMP kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 260	260	Uridylate kinase HAMAP MF_01220		PRO_0000143898
Regions
	Nucleotide binding	29 – 32	4	ATP By similarity
	Nucleotide binding	152 – 159	8	UMP By similarity
	Region	37 – 42	6	Involved in allosteric activation by GTP Potential
Sites
	Binding site	71	1	UMP; via amide nitrogen By similarity
	Binding site	72	1	ATP; via amide nitrogen By similarity
	Binding site	76	1	ATP By similarity
	Binding site	91	1	UMP By similarity
	Binding site	179	1	ATP By similarity
	Binding site	185	1	ATP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	188	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P74457-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: E234C011E3CB4C98	FASTA	260	27,651
	10 20 30 40 50 60 MGGILRLTLI PCLYINGDGG MSYQRVLLKL SGEALMGDLG YGIDPAVVGT IAQEIKDVLQ 70 80 90 100 110 120 AGVQLAIVVG GGNIFRGVKA SAAGMDRATA DYIGMIATVM NAMTLQDALE QMDIPTRVLT 130 140 150 160 170 180 AIAMQEVAEP YIRRRAIRHL EKGRVVIFGA GSGNPFFTTD TTAALRAAEI DAEVVFKATK 190 200 210 220 230 240 VDGVYDSDPK TNPNARRFTT LTYSHVLAED LKVMDSTAIA LCKDNNIPIM IFDLGVPGNI 250 260 VRAIKGEAVG TLVGENCEVS

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000022 Genomic DNA. Translation: BAA18558.1.
PIR	S76429.
RefSeq	NP_441880.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P74457. 3 interactions.
STRING	P74457.
Genome annotation databases
GeneID	952864.
GenomeReviews	Gene locus sll0144 in contig BA000022_GR.
KEGG	syn:sll0144.
NMPDR	fig|1148.1.peg.1981.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG497552.
OMA	RHMEKGR.
Enzyme and pathway databases
BioCyc	SSP1148:SLL0144-MON.
Family and domain databases
HAMAP	MF_01220. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR011817. Uridylate_kinase. IPR015963. Uridylate_kinase_bac. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF005650. Uridylate_kin. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PYRH_SYNY3

Accession

Primary (citable) accession number: P74457

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	December 15, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)