P74457 (PYRH_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uridylate kinase
Short name=UK
EC=2.7.4.22

Alternative name(s):
Uridine monophosphate kinase
Short name=UMP kinase
Short name=UMPK

Gene names

Name:	pyrH
Ordered Locus Names:	sll0144

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa)

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible phosphorylation of UMP to UDP By similarity. HAMAP MF_01220_B
Catalytic activity	ATP + UMP = ADP + UDP. HAMAP MF_01220_B
Enzyme regulation	Allosterically activated by GTP. Inhibited by UTP By similarity. HAMAP MF_01220_B
Pathway	Pyrimidine metabolism; CTP biosynthesis via de novo pathway; UDP from UMP (UMPK route): step 1/1. HAMAP MF_01220_B
Subunit structure	Homohexamer By similarity. HAMAP MF_01220_B
Subcellular location	Cytoplasm By similarity HAMAP MF_01220_B.
Sequence similarities	Belongs to the UMP kinase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular amino acid biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UMP kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 260	260	Uridylate kinase HAMAP MF_01220_B		PRO_0000143898
Regions
	Nucleotide binding	29 – 32	4	ATP By similarity
	Nucleotide binding	152 – 159	8	UMP By similarity
	Region	37 – 42	6	Involved in allosteric activation by GTP Potential
Sites
	Binding site	71	1	UMP; via amide nitrogen By similarity
	Binding site	72	1	ATP; via amide nitrogen By similarity
	Binding site	76	1	ATP By similarity
	Binding site	91	1	UMP By similarity
	Binding site	179	1	ATP By similarity
	Binding site	185	1	ATP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	188	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P74457 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: E234C011E3CB4C98	FASTA	260	27,651
	10 20 30 40 50 60 MGGILRLTLI PCLYINGDGG MSYQRVLLKL SGEALMGDLG YGIDPAVVGT IAQEIKDVLQ 70 80 90 100 110 120 AGVQLAIVVG GGNIFRGVKA SAAGMDRATA DYIGMIATVM NAMTLQDALE QMDIPTRVLT 130 140 150 160 170 180 AIAMQEVAEP YIRRRAIRHL EKGRVVIFGA GSGNPFFTTD TTAALRAAEI DAEVVFKATK 190 200 210 220 230 240 VDGVYDSDPK TNPNARRFTT LTYSHVLAED LKVMDSTAIA LCKDNNIPIM IFDLGVPGNI 250 260 VRAIKGEAVG TLVGENCEVS

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA18558.1.
PIR	S76429.
3D structure databases
ProteinModelPortal	P74457.
SMR	P74457. Positions 21-253.
ModBase	Search...
Protein-protein interaction databases
IntAct	P74457. 3 interactions.
STRING	P74457.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GenomeReviews	Gene locus sll0144 in contig BA000022_GR.
KEGG	syn:sll0144.
NMPDR	fig|1148.1.peg.1981.
Phylogenomic databases
eggNOG	COG0528.
HOGENOM	HBG497552.
OMA	RHMEKGR.
PhylomeDB	P74457.
ProtClustDB	PRK00358.
Enzyme and pathway databases
BioCyc	SSP1148:SLL0144-MONOMER.
Family and domain databases
HAMAP	MF_01220_B. PyrH_B. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR011817. Uridylate_kinase. IPR015963. Uridylate_kinase_bac. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
KO	K09903.
PANTHER	PTHR26059. PTHR26059. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF005650. Uridylate_kin. 1 hit.
SUPFAM	SSF53633. Aa_kinase. 1 hit.
TIGRFAMs	TIGR02075. PyrH_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PYRH_SYNY3

Accession

Primary (citable) accession number: P74457

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program