P74438 (PYRC_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroorotase
Short name=DHOase
EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	slr0406

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa)

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate. HAMAP MF_00219
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_00219
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3. HAMAP MF_00219
Subunit structure	Homodimer By similarity. HAMAP MF_00219
Sequence similarities	Belongs to the DHOase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 342	342	Dihydroorotase HAMAP MF_00219		PRO_0000147220
Sites
	Metal binding	13	1	Zinc 1 By similarity
	Metal binding	15	1	Zinc 1 By similarity
	Metal binding	99	1	Zinc 1; via carbamate group By similarity
	Metal binding	99	1	Zinc 2; via carbamate group By similarity
	Metal binding	136	1	Zinc 2 By similarity
	Metal binding	174	1	Zinc 2 By similarity
	Metal binding	246	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	99	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P74438 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: E8A572814296B121	FASTA	342	38,127
	10 20 30 40 50 60 MEKLTITRPD DWHLHLRDGA ALKAVLPHTV RQFARAIVMP NLKPPVRSVA DAAAYRERIL 70 80 90 100 110 120 AAIPAGGQFE PLMTLYLTDN TNPEEIIAAK ASQFVKAVKY YPAGATTNSD FGVTDIHRCD 130 140 150 160 170 180 AVLAAMEQVD LPLLLHGEVT DSNIDIFDRE KVFIEKYLIP LREKFPRLRV VLEHITTSDA 190 200 210 220 230 240 VQFVLSANNI AATITPQHLL FSRNALFKGG ICPHFYCLPI LKREEHRLSL LHAATSGNPK 250 260 270 280 290 300 FFLGTDSAPH ARNSKESLCG CAGCYSALHA MELYAEAFES VNSLDKLEAF ASFYGPDFYQ 310 320 330 340 LPRNTAQITL MKNPWRIPAE LPFPESGLVP LRAGEEITWQ MV

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA18539.1.
PIR	S76410.
RefSeq	NP_441861.1. NC_000911.1.
3D structure databases
ProteinModelPortal	P74438.
SMR	P74438. Positions 2-341.
ModBase	Search...
Protein-protein interaction databases
IntAct	P74438. 3 interactions.
STRING	P74438.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	954248.
GenomeReviews	Gene locus slr0406 in contig BA000022_GR.
KEGG	syn:slr0406.
NMPDR	fig|1148.1.peg.1962.
PATRIC	23841248. VBISynSp132158_2174.
Phylogenomic databases
eggNOG	COG0418.
HOGENOM	HBG628648.
OMA	CLPVAKR.
PhylomeDB	P74438.
ProtClustDB	PRK05451.
Enzyme and pathway databases
BioCyc	SSP1148:SLR0406-MONOMER.
Family and domain databases
HAMAP	MF_00219. PyrC_type1. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004721. DHOdimr. IPR002195. Dihydroorotase_CS. [Graphical view]
KO	K01465.
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
PIRSF	PIRSF001237. DHOdimr. 1 hit.
TIGRFAMs	TIGR00856. PyrC_dimer. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_SYNY3

Accession

Primary (citable) accession number: P74438

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program