P74432 (NTPA_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

slr0402

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa)

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	194 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 194	194	Non-canonical purine NTP pyrophosphatase HAMAP MF_01405		PRO_0000178251
Regions
	Region	8 – 13	6	Substrate binding By similarity
	Region	67 – 68	2	Substrate binding By similarity
Sites
	Metal binding	38	1	Magnesium or manganese By similarity
	Metal binding	67	1	Magnesium or manganese By similarity
	Binding site	149	1	Substrate By similarity
	Binding site	169	1	Substrate By similarity
	Binding site	175	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P74432 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: F6BBF1AF46DBB28B	FASTA	194	21,188
	10 20 30 40 50 60 MPTLIVATGN PGKLAEMQTY LEPLGCQLTL KPTEIEVEET GSTFYENACL KASQVAKAVN 70 80 90 100 110 120 QWAIADDSGL AVDALDGAPG LYSARYGNTD RERIAKLLQA LEGVSQRQAQ FICVVSIAAP 130 140 150 160 170 180 DGSIQLSTKG ICSGEITHSP RGDQGFGYDP IFWLPEHKKT FAEMTKVEKQ KVSHRGKAFN 190 KLIHQWPGLK FDSF

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA18532.1.
PIR	S76403.
RefSeq	NP_441854.1. NC_000911.1.
3D structure databases
ProteinModelPortal	P74432.
ModBase	Search...
Protein-protein interaction databases
STRING	P74432.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	952877.
GenomeReviews	Gene locus slr0402 in contig BA000022_GR.
KEGG	syn:slr0402.
NMPDR	fig|1148.1.peg.1955.
PATRIC	23841228. VBISynSp132158_2164.
Phylogenomic databases
eggNOG	COG0127.
HOGENOM	HBG697237.
OMA	ESSTRLT.
PhylomeDB	P74432.
ProtClustDB	PRK00120.
Enzyme and pathway databases
BioCyc	SSP1148:SLR0402-MONOMER.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NTPA_SYNY3

Accession

Primary (citable) accession number: P74432

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program