Reviewed, UniProtKB/Swiss-Prot P74423 (SYA_SYNY3)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	sll0362

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	877 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Subcellular location	Cytoplasm.
Domain	The C-terminal C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 877	877	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000075229

Sequences

	Sequence		Length	Mass (Da)
	P74423-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 2E68212CA2579A77	FASTA	877	96,141
	10 20 30 40 50 60 MTTTPPVLSG PEIRQQFLNF FADRQHQILP SASLVPEDPT VLLTIAGMLP FKPIFLGQKS 70 80 90 100 110 120 AEFPRATTSQ KCIRTNDIEN VGRTARHHTF FEMLGNFSFG DYFKSQAIAW AWELSTQVFK 130 140 150 160 170 180 LPAERLVVSV FEEDDEAFAI WRDEIGIPAH RIQRMGADDN FWVSGPTGPC GPCSEIYYDF 190 200 210 220 230 240 HPELGDEKLD LEDDSRFIEF YNLVFMQYNR DNAGNLTPLE KKNIDTGMGL ERMAQILQKV 250 260 270 280 290 300 PNNYETDLIF PIIQTAANIA GIDYAQANEK TKVSLKVIGD HVRSVVHMIA DGISASNLGR 310 320 330 340 350 360 GYVLRRLIRR VVRHGRLLGI NGEFTTKVAA TAVQLAQPVY PNVLERQSLI EQELQREEAA 370 380 390 400 410 420 FLKTLERGEK LLADLMADGV TEIAGADAFT LYDTFGFPLE LTQEIAEEQG ITVDVEGFEK 430 440 450 460 470 480 AMQEQQERSK AAHETIDLTV QESLDKLANH IHPTEFLGYT DLQSSAIVKA VLVGGELVDQ 490 500 510 520 530 540 AVAGQTVQIV LDQTPFYGES GGQIGDKGFL NGDNLLIRIE DVKRESGIFI HFGRVERGTV 550 560 570 580 590 600 QIGTTITATI DRACRRRAQA NHTATHLLQS ALKRVVDEGI SQAGSLVDFN RLRFDFNSPR 610 620 630 640 650 660 AVTMEELQQI EDLINQWIAE AHQTEVAVMP IADAKAKGAI AMFGEKYGAE VRVIDVPGVS 670 680 690 700 710 720 LELCGGTHVA NTAEIGLFKI VAETGIAAGV RRIEAVAGPS VLDYLNVREA VVKELGDRLK 730 740 750 760 770 780 AKPEEIPDRV HQLQQELKAS QKQLEALKQE LALQKSEQLL TQAQTVGEFK ILVADLGTVD 790 800 810 820 830 840 GESLKTAAER LQQKLGESAV VLASIPEEGK VSLVAAFSPQ LVKTKQLKAG QFIGAIAKIC 850 860 870 GGGGGGRPNL AQAGGRDASK LPEALATAKQ TLLAELG

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000022 Genomic DNA. Translation: BAA18523.1.
PIR	S76394.
RefSeq	NP_441845.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P74423. 1 interaction.
STRING	P74423.
Genome annotation databases
GeneID	953136.
GenomeReviews	Gene locus sll0362 in contig BA000022_GR.
KEGG	syn:sll0362.
NMPDR	fig|1148.1.peg.1946.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P74423.
OMA	GHTIEPS
Enzyme and pathway databases
BioCyc	SSP1148:SLL0362-MON.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_cons-reg. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_SYNY3

Accession

Primary (citable) accession number: P74423

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	November 24, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)