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P74397 (PRIA_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Primosomal protein N'

EC=3.6.4.-
Alternative name(s):
ATP-dependent helicase priA
Replication factor Y
Gene names
Name:priA
Ordered Locus Names:sll0270
OrganismSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifier1111708 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaChroococcalesSynechocystis

Protein attributes

Sequence length831 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase within the primosome By similarity.

Sequence similarities

Belongs to the helicase family. PriA subfamily.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 831831Primosomal protein N'
PRO_0000102134

Regions

Domain304 – 471168Helicase ATP-binding
Domain575 – 735161Helicase C-terminal
Nucleotide binding317 – 3248ATP Potential
Zinc finger537 – 54913C4-type Potential
Zinc finger568 – 58417C4-type Potential
Motif413 – 4164DEEH box

Sequences

Sequence LengthMass (Da)Tools
P74397 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: EA52CDF95654D94A

FASTA83193,307
        10         20         30         40         50         60 
MTVSPSALAE LGFNYQEEDS VRPWAAVLVD LPQNEGVYTY AIPPGLTVQD GDIVAVPFGN 

        70         80         90        100        110        120 
QQLGGIVVGC LTKLPPDLPP EKIKPIQDVI VSGFFAPHYW RLLHWLAEYY CTELITVIRM 

       130        140        150        160        170        180 
ALPPGLLQRS QRRIKLNGDR LPSDWPLFLS QPSHQGARQI LTLLQSSKEG DYSYRYLRQK 

       190        200        210        220        230        240 
VPGLTKALRD LLKRQWVESY LEPPKAVQPQ LPKMVTLLNF DPSFKLTELQ ARTLIVLKNQ 

       250        260        270        280        290        300 
GGEMWLADLL KAVPCSASTI QSLAKKGLVA IAEREKLRLF QQPSINASQA PELTPAQKQA 

       310        320        330        340        350        360 
CQTVLPLQGY HQVLLHGVTG SGKTEVYLQI CGDRLGKGQS VLVLVPEIGL TPQLTDRFRA 

       370        380        390        400        410        420 
RFGNKVAVYH SGLSSGEKYD TWRQTLLGHE QIVIGTRSAI FTPLPNLGLI ILDEEHDSSF 

       430        440        450        460        470        480 
KQTQLTPNYH ARTVAQRRAE LEQCPLILGS ATPSLESWHT VHRHQNDPQR HYLELPERVQ 

       490        500        510        520        530        540 
SRPLPPVQIV DMRAELKQGN RSIFSRALQT ALGELKAKQQ QGILFINRRG HSTFVSCRSC 

       550        560        570        580        590        600 
GYVLECPNCD VSLSYHYVQG NGQPLLRCHY CNHAEIQPKV CPECSSPYLK FFGSGTQKVT 

       610        620        630        640        650        660 
EALTQEFPDL RWIRFDSDTT RRKGAHRALL DQFQRGEADL LVGTQMLTKG LDLAQITLVG 

       670        680        690        700        710        720 
VVAADSLLNF ADYRSAERGF QTLTQVSGRA GRGEEPGQVI IQTYTPRHPV IQAVKTHNYH 

       730        740        750        760        770        780 
GFIAQELPQR EMLNYPPYGK LILLRCLGTN EREVEKTIQA IAVLCEQLLP KTVEILGPAP 

       790        800        810        820        830 
ASILRIAQRY RWQLLVKYPS FIKVILPLER IKQICPSSVY LDIDVDPLSI D 

« Hide

References

[1]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. expand/collapse author list , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27184 / PCC 6803 / N-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000022 Genomic DNA. Translation: BAA18494.1.
PIRS76235.
RefSeqNP_441816.1. NC_000911.1.

3D structure databases

ProteinModelPortalP74397.
ModBaseSearch...

Protein-protein interaction databases

IntActP74397. 5 interactions.
STRINGP74397.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID952898.
GenomeReviewsGene locus sll0270 in contig BA000022_GR.
KEGGsyn:sll0270.
NMPDRfig|1148.1.peg.1917.
PATRIC23841138. VBISynSp132158_2120.

Phylogenomic databases

eggNOGCOG1198.
HOGENOMHBG725563.
OMAVSLSYHY.
PhylomeDBP74397.
ProtClustDBPRK05580.

Enzyme and pathway databases

BioCycSSP1148:SLL0270-MONOMER.

Family and domain databases

InterProIPR014001. DEAD-like_helicase.
IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR001650. Helicase_C.
IPR005259. PriA.
[Graphical view]
KOK04066.
PfamPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
TIGRFAMsTIGR00595. PriA. 1 hit.
PROSITEPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePRIA_SYNY3
AccessionPrimary (citable) accession number: P74397
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: January 25, 2012
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Synechocystis PCC 6803

Synechocystis (strain PCC 6803): entries and gene names

SIMILARITY comments

Index of protein domains and families