P74397 (PRIA_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Primosomal protein N'
EC=3.6.4.-

Alternative name(s):
ATP-dependent helicase priA
Replication factor Y

Gene names

Name:	priA
Ordered Locus Names:	sll0270

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa)

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	831 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase within the primosome By similarity.
Sequence similarities	Belongs to the helicase family. PriA subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Primosome
Domain	Zinc-finger
Ligand	ATP-binding DNA-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Helicase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA replication, synthesis of RNA primer Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	alpha DNA polymerase:primase complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 831	831	Primosomal protein N'		PRO_0000102134
Regions
	Domain	304 – 471	168	Helicase ATP-binding
	Domain	575 – 735	161	Helicase C-terminal
	Nucleotide binding	317 – 324	8	ATP Potential
	Zinc finger	537 – 549	13	C4-type Potential
	Zinc finger	568 – 584	17	C4-type Potential
	Motif	413 – 416	4	DEEH box

Sequences

	Sequence		Length	Mass (Da)
	P74397 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: EA52CDF95654D94A	FASTA	831	93,307
	10 20 30 40 50 60 MTVSPSALAE LGFNYQEEDS VRPWAAVLVD LPQNEGVYTY AIPPGLTVQD GDIVAVPFGN 70 80 90 100 110 120 QQLGGIVVGC LTKLPPDLPP EKIKPIQDVI VSGFFAPHYW RLLHWLAEYY CTELITVIRM 130 140 150 160 170 180 ALPPGLLQRS QRRIKLNGDR LPSDWPLFLS QPSHQGARQI LTLLQSSKEG DYSYRYLRQK 190 200 210 220 230 240 VPGLTKALRD LLKRQWVESY LEPPKAVQPQ LPKMVTLLNF DPSFKLTELQ ARTLIVLKNQ 250 260 270 280 290 300 GGEMWLADLL KAVPCSASTI QSLAKKGLVA IAEREKLRLF QQPSINASQA PELTPAQKQA 310 320 330 340 350 360 CQTVLPLQGY HQVLLHGVTG SGKTEVYLQI CGDRLGKGQS VLVLVPEIGL TPQLTDRFRA 370 380 390 400 410 420 RFGNKVAVYH SGLSSGEKYD TWRQTLLGHE QIVIGTRSAI FTPLPNLGLI ILDEEHDSSF 430 440 450 460 470 480 KQTQLTPNYH ARTVAQRRAE LEQCPLILGS ATPSLESWHT VHRHQNDPQR HYLELPERVQ 490 500 510 520 530 540 SRPLPPVQIV DMRAELKQGN RSIFSRALQT ALGELKAKQQ QGILFINRRG HSTFVSCRSC 550 560 570 580 590 600 GYVLECPNCD VSLSYHYVQG NGQPLLRCHY CNHAEIQPKV CPECSSPYLK FFGSGTQKVT 610 620 630 640 650 660 EALTQEFPDL RWIRFDSDTT RRKGAHRALL DQFQRGEADL LVGTQMLTKG LDLAQITLVG 670 680 690 700 710 720 VVAADSLLNF ADYRSAERGF QTLTQVSGRA GRGEEPGQVI IQTYTPRHPV IQAVKTHNYH 730 740 750 760 770 780 GFIAQELPQR EMLNYPPYGK LILLRCLGTN EREVEKTIQA IAVLCEQLLP KTVEILGPAP 790 800 810 820 830 ASILRIAQRY RWQLLVKYPS FIKVILPLER IKQICPSSVY LDIDVDPLSI D

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA18494.1.
PIR	S76235.
RefSeq	NP_441816.1. NC_000911.1.
3D structure databases
ProteinModelPortal	P74397.
ModBase	Search...
Protein-protein interaction databases
IntAct	P74397. 5 interactions.
STRING	P74397.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	952898.
GenomeReviews	Gene locus sll0270 in contig BA000022_GR.
KEGG	syn:sll0270.
NMPDR	fig|1148.1.peg.1917.
PATRIC	23841138. VBISynSp132158_2120.
Phylogenomic databases
eggNOG	COG1198.
HOGENOM	HBG725563.
OMA	VSLSYHY.
PhylomeDB	P74397.
ProtClustDB	PRK05580.
Enzyme and pathway databases
BioCyc	SSP1148:SLL0270-MONOMER.
Family and domain databases
InterPro	IPR014001. DEAD-like_helicase. IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR001650. Helicase_C. IPR005259. PriA. [Graphical view]
KO	K04066.
Pfam	PF00270. DEAD. 1 hit. PF00271. Helicase_C. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00595. PriA. 1 hit.
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PRIA_SYNY3

Accession

Primary (citable) accession number: P74397

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program