P74384 (PUR8_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenylosuccinate lyase
Short name=ASL
EC=4.3.2.2

Alternative name(s):
Adenylosuccinase
Short name=ASase

Gene names

Name:	purB
Ordered Locus Names:	sll0421

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa)

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	N(6)-(1,2-dicarboxyethyl)AMP = fumarate + AMP. (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2. Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2.
Sequence similarities	Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	purine ribonucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate AMP-lyase (fumarate-forming) activity Inferred from electronic annotation. Source: EC N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 431	431	Adenylosuccinate lyase		PRO_0000137885
Sites
	Active site	68	1	Proton donor By similarity
	Active site	141	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P74384 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 38811A661A62BD07	FASTA	431	48,420
	10 20 30 40 50 60 MIERYTLPEM GKIWTDTYKL QTWLDVEIAV CEAQAELGYI PQAAVDEIKA KAKFDPQRVL 70 80 90 100 110 120 EIEAEVRHDV IAFLTNVNEY VGDAGRYIHL GLTSSDVLDT ALALQLVASL DLILEQLEKL 130 140 150 160 170 180 IQAIRYQAQQ HRYTVMVGRS HGIHAEPITF GFKLAGWLAE VLRNRDRLVR VAQSIAVGKI 190 200 210 220 230 240 SGAVGTYANI DPKVEAIACQ KLGLEPDTAS TQVISRDRHA EYVQQLALLA ASLERFAVEI 250 260 270 280 290 300 RNLQRTDVLE VEEYFSKGQK GSSAMPHKRN PIRSERLTGM ARLVRGHAVA ALENVALWHE 310 320 330 340 350 360 RDISHSSVER VAFPDCCILT HFMLKETTDL VKNLLVYPEN MKRNMNVYGG VIFSQKVLLA 370 380 390 400 410 420 LVEKGMNRED AYRVVQGSAH QAWNTEGGNF EELVRADAQV TALLSAEEID QCFDPQQHLT 430 NLAEIYSRLD I

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA18481.1.
PIR	S76222.
RefSeq	NP_441803.1. NC_000911.1.
3D structure databases
ProteinModelPortal	P74384.
ModBase	Search...
Protein-protein interaction databases
IntAct	P74384. 1 interaction.
STRING	P74384.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	952910.
GenomeReviews	Gene locus sll0421 in contig BA000022_GR.
KEGG	syn:sll0421.
NMPDR	fig|1148.1.peg.1904.
PATRIC	23841108. VBISynSp132158_2105.
Phylogenomic databases
eggNOG	COG0015.
HOGENOM	HBG499008.
OMA	KEVKHDV.
PhylomeDB	P74384.
ProtClustDB	PRK07380.
Enzyme and pathway databases
BioCyc	SSP1148:SLL0421-MONOMER.
Family and domain databases
InterPro	IPR019468. AdenyloSucc_lyase_C. IPR003031. D_crystallin. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. IPR008948. L-Aspartase-like. IPR024083. L-Aspartase-like_N. IPR022761. Lyase1_N. IPR004769. Pur_lyase. [Graphical view]
Gene3D	G3DSA:1.10.275.10. G3DSA:1.10.275.10. 1 hit.
KO	K01756.
PANTHER	PTHR11444:SF2. PTHR11444:SF2. 1 hit.
Pfam	PF10397. ADSL_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00145. ARGSUCLYASE. PR00149. FUMRATELYASE.
SMART	SM00998. ADSL_C. 1 hit. [Graphical view]
SUPFAM	SSF48557. L-Aspartase-like. 1 hit.
TIGRFAMs	TIGR00928. PurB. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR8_SYNY3

Accession

Primary (citable) accession number: P74384

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program