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Protein

Cysteine--tRNA ligase

Gene

cysS

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi29 – 291ZincBy similarity
Metal bindingi215 – 2151ZincBy similarity
Metal bindingi240 – 2401ZincBy similarity
Metal bindingi244 – 2441ZincBy similarity
Binding sitei275 – 2751ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine--tRNA ligase (EC:6.1.1.16)
Alternative name(s):
Cysteinyl-tRNA synthetase
Short name:
CysRS
Gene namesi
Name:cysS
Ordered Locus Names:slr0958
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
Proteomesi
  • UP000001425 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 483483Cysteine--tRNA ligasePRO_0000159505Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

IntActiP74330. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP74330.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi31 – 4111"HIGH" regionAdd
BLAST
Motifi272 – 2765"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000245250.
InParanoidiP74330.
KOiK01883.
OMAiHENEACQ.
OrthoDBiEOG6RVFXC.
PhylomeDBiP74330.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00041. Cys_tRNA_synth.
InterProiIPR015803. Cys-tRNA-ligase.
IPR015273. Cys-tRNA-synt_Ia_DALR.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF09190. DALR_2. 1 hit.
PF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
SMARTiSM00840. DALR_2. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00435. cysS. 1 hit.

Sequencei

Sequence statusi: Complete.

P74330-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLRLYDSLT RAKQPLQPLH PGLVTMYCCG ITVYDYCHLG HARTCITWDL
60 70 80 90 100
VRRYLQWSGY EVRYVQNFTD IDDKILKRAV DESSTMEAVS EKFIAAYFED
110 120 130 140 150
MEALGVQPAD LYPRATHTLD GIKRLIAELE AKGYAYPSGG DVYYSVRNFD
160 170 180 190 200
GYGKLSGRRL EDLQAGASGR VTVADPDGAQ KQDPFDFALW KSAKPGEPAW
210 220 230 240 250
ESPWGKGRPG WHIECSAMVR EFLGETIDLH VGGNDLIFPH HENEIAQSEA
260 270 280 290 300
VTGQPLAQYW LHNGMVKVDG EKMSKSLGNF TTIRALLKTV DPMAIRLLVL
310 320 330 340 350
QGHYRKPLDF TETAIAAATN GWHTLREGLQ FGYDYGGDFG WELAFTPLGP
360 370 380 390 400
DANQWTVAFQ EAVDDDFNFA GGLAVMFELA KHLRSEGNKL KFEGSTPADL
410 420 430 440 450
DLLQTQWITL VSLAEILGLS INPESQSSND NGLTDGDIEQ LVAQRTQARK
460 470 480
DKNWAEGDRL RDVLQEAGIT LVDKPGGLTE WFR
Length:483
Mass (Da):53,968
Last modified:February 1, 1997 - v1
Checksum:i6F200064977A3A5D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA18424.1.
PIRiS76165.

Genome annotation databases

EnsemblBacteriaiBAA18424; BAA18424; BAA18424.
KEGGisyn:slr0958.
PATRICi23840970. VBISynSp132158_2039.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA18424.1.
PIRiS76165.

3D structure databases

ProteinModelPortaliP74330.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP74330. 1 interaction.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA18424; BAA18424; BAA18424.
KEGGisyn:slr0958.
PATRICi23840970. VBISynSp132158_2039.

Phylogenomic databases

HOGENOMiHOG000245250.
InParanoidiP74330.
KOiK01883.
OMAiHENEACQ.
OrthoDBiEOG6RVFXC.
PhylomeDBiP74330.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00041. Cys_tRNA_synth.
InterProiIPR015803. Cys-tRNA-ligase.
IPR015273. Cys-tRNA-synt_Ia_DALR.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF09190. DALR_2. 1 hit.
PF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
SMARTiSM00840. DALR_2. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00435. cysS. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.

Entry informationi

Entry nameiSYC_SYNY3
AccessioniPrimary (citable) accession number: P74330
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: July 6, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.