P74325 (P74325_SYNY3) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Slr0953 protein EMBL BAA18419.1

Gene names

Ordered Locus Names:

slr0953

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa)

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	Magnesium PDB 1S2O PDB 1TJ5 PDB 2B1Q PDB 2B1R PDB 2D2V PDB 1TJ4 PDB 1U2S PDB 1TJ3 Metal-binding PDB 1S2O PDB 1TJ5 PDB 2B1Q PDB 2B1R PDB 2D2V PDB 1TJ4 PDB 1U2S PDB 1TJ3
Technical term	3D-structure PDB 1S2O PDB 1TJ5 PDB 2B1Q PDB 2B1R PDB 2D2V PDB 1TJ4 PDB 1U2S PDB 1U2T PDB 1TJ3 Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	sucrose biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: InterPro sucrose-phosphate phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	152 – 155	4	Sucrose binding PDB 1TJ5 PDB 1TJ4
Sites
	Metal binding	9	1	Magnesium 1 PDB 1S2O PDB 1TJ5
	Metal binding	9	1	Magnesium 2 PDB 1TJ5 PDB 2B1Q PDB 2B1R PDB 2D2V
	Metal binding	11	1	Magnesium 1; via carbonyl oxygen PDB 1S2O PDB 1TJ5
	Metal binding	186	1	Magnesium 1 PDB 1S2O PDB 1TJ5
	Metal binding	186	1	Magnesium 2 PDB 1TJ5 PDB 2B1Q PDB 2B1R PDB 2D2V
	Metal binding	187	1	Magnesium 2 PDB 1TJ5 PDB 2B1Q PDB 2B1R PDB 2D2V
	Metal binding	189	1	Magnesium 2 PDB 1TJ5 PDB 2B1Q PDB 2B1R PDB 2D2V
	Metal binding	190	1	Magnesium 2 PDB 1TJ5 PDB 2B1Q PDB 2B1R PDB 2D2V
	Binding site	107	1	Glucose PDB 1U2S
	Binding site	107	1	Sucrose PDB 1TJ5 PDB 1TJ4
	Binding site	111	1	Glucose PDB 1U2S
	Binding site	111	1	Sucrose PDB 1TJ5 PDB 1TJ4
	Binding site	116	1	Glucose PDB 1U2S
	Binding site	116	1	Sucrose PDB 1TJ5 PDB 1TJ4
	Binding site	155	1	Glucose PDB 1U2S
	Binding site	189	1	Glucose PDB 1U2S
	Binding site	189	1	Sucrose PDB 1TJ5 PDB 1TJ4

Sequences

	Sequence		Length	Mass (Da)
	P74325 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 0D4D7AC50AC4FF71	FASTA	244	27,762
	10 20 30 40 50 60 MRQLLLISDL DNTWVGDQQA LEHLQEYLGD RRGNFYLAYA TGRSYHSARE LQKQVGLMEP 70 80 90 100 110 120 DYWLTAVGSE IYHPEGLDQH WADYLSEHWQ RDILQAIADG FEALKPQSPL EQNPWKISYH 130 140 150 160 170 180 LDPQACPTVI DQLTEMLKET GIPVQVIFSS GKDVDLLPQR SNKGNATQYL QQHLAMEPSQ 190 200 210 220 230 240 TLVCGDSGND IGLFETSARG VIVRNAQPEL LHWYDQWGDS RHYRAQSSHA GAILEAIAHF DFLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.
[2]	"The structure of a cyanobacterial sucrose-phosphatase reveals the sugar tongs that release free sucrose in the cell." Fieulaine S., Lunn J.E., Borel F., Ferrer J.L. Plant Cell 17:2049-2058(2005) [PubMed: 15937230] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS) IN COMPLEX WITH GLUCOSE; MAGNESIUM AND SUCROSE.
[3]	"Crystal structure of a cyanobacterial sucrose-phosphatase in complex with glucose-containing disaccharides." Fieulaine S., Lunn J.E., Ferrer J.L. Proteins 68:796-801(2007) [PubMed: 17510968] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) IN COMPLEX WITH MAGNESIUM.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BA000022 Genomic DNA. Translation: BAA18419.1.

PIR

S76160.

RefSeq

NP_441739.1. NC_000911.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1S2O	X-ray	1.40	A	1-244	[»]
1TJ3	X-ray	2.80	A	1-244	[»]
1TJ4	X-ray	2.70	A	1-244	[»]
1TJ5	X-ray	2.20	A	1-244	[»]
1U2S	X-ray	2.50	A	1-244	[»]
1U2T	X-ray	2.90	A	1-244	[»]
2B1Q	X-ray	2.20	A	1-244	[»]
2B1R	X-ray	2.20	A	1-244	[»]
2D2V	X-ray	2.50	A	1-244	[»]

ProteinModelPortal

P74325.

SMR

P74325. Positions 1-244.

ModBase

Search...

Protein-protein interaction databases

IntAct

P74325. 1 interaction.

STRING

P74325.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

952967.

GenomeReviews

Gene locus slr0953 in contig BA000022_GR.

KEGG

syn:slr0953.

NMPDR

fig|1148.1.peg.1840.

PATRIC

23840960. VBISynSp132158_2034.

Phylogenomic databases

eggNOG

COG0561.

HOGENOM

HBG285174.

OMA

WESSAKP.

PhylomeDB

P74325.

ProtClustDB

CLSK2461515.

Enzyme and pathway databases

BioCyc

SSP1148:SLR0953-MONOMER.

Family and domain databases

InterPro

IPR023214. HAD-like_dom.
IPR006379. HAD-SF_hydro_IIB.
IPR006378. Suc_phosP.
IPR006380. Sucrose-P_synthase.
IPR012847. Sucrose_phosphatase_pln/cyn.
[Graphical view]

Gene3D

G3DSA:3.40.50.1000. HAD-like_dom. 2 hits.

KO

K07024.

Pfam

PF05116. S6PP. 1 hit.
[Graphical view]

SUPFAM

SSF56784. HAD-like_dom. 1 hit.

TIGRFAMs

TIGR01484. HAD-SF-IIB. 1 hit.
TIGR01482. SPP-subfamily. 1 hit.
TIGR01485. SPP_plant-cyano. 1 hit.

ProtoNet

Search...

Entry information

Entry name

P74325_SYNY3

Accession

Primary (citable) accession number: P74325

Entry history

Integrated into UniProtKB/TrEMBL:	February 1, 1997
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)