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Protein

Phenylalanine--tRNA ligase beta subunit

Gene

pheT

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per tetramer.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi473 – 4731MagnesiumBy similarity
Metal bindingi479 – 4791Magnesium; via carbonyl oxygenBy similarity
Metal bindingi482 – 4821MagnesiumBy similarity
Metal bindingi483 – 4831MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylalanine--tRNA ligase beta subunit (EC:6.1.1.20)
Alternative name(s):
Phenylalanyl-tRNA synthetase beta subunit
Short name:
PheRS
Gene namesi
Name:pheT
Ordered Locus Names:sll1553
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 810810Phenylalanine--tRNA ligase beta subunitPRO_0000126974Add
BLAST

Proteomic databases

PaxDbiP74296.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.By similarity

Protein-protein interaction databases

IntActiP74296. 4 interactions.

Structurei

3D structure databases

ProteinModelPortaliP74296.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 150112tRNA-bindingAdd
BLAST
Domaini407 – 49589B5Add
BLAST
Domaini716 – 80994FDX-ACBAdd
BLAST

Sequence similaritiesi

Contains 1 B5 domain.Curated
Contains 1 FDX-ACB domain.Curated
Contains 1 tRNA-binding domain.Curated

Phylogenomic databases

eggNOGiCOG0072.
HOGENOMiHOG000292087.
InParanoidiP74296.
KOiK01890.
OMAiMKFSEQW.
OrthoDBiEOG6CCH1J.
PhylomeDBiP74296.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.56.20. 1 hit.
3.30.70.380. 1 hit.
3.50.40.10. 1 hit.
HAMAPiMF_00283. Phe_tRNA_synth_beta1.
InterProiIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR012340. NA-bd_OB-fold.
IPR004532. Phe-tRNA-ligase_IIc_bsu.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR005121. PheS_beta_Fdx_antiC-bd.
IPR002547. tRNA-bd_dom.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
PfamiPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
PF03147. FDX-ACB. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
SMARTiSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
SM00896. FDX-ACB. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54991. SSF54991. 1 hit.
SSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR00472. pheT_bact. 1 hit.
PROSITEiPS51483. B5. 1 hit.
PS51447. FDX_ACB. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P74296-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRISVNWLQS LVELNLSPEE LGELLTIAGL EVEEIEDRRS WAAGVVLGRV
60 70 80 90 100
ISREKHPNAD KLSVCVVDIG TEEPSTIVCG AANVRADILV PVATLGSYLP
110 120 130 140 150
KVDLKIKPAK LRGVKSSGMI CSLAELGLSK ESEGIHIFPD LDLPSGSPVG
160 170 180 190 200
PLLGLDDVIL EISPTANRAD ALSMVGVARE VAALTGGKLS LPEIKAVSVS
210 220 230 240 250
DQDLPISVTE PQACPTYVGT VIRGVKVGPS PDWLQQRLLA AGTRPINNVV
260 270 280 290 300
DVTNYVLLEW GQPLHSFDQD KLQTLVGPEG FALGVRFAEE GEKLITLDDQ
310 320 330 340 350
ERTLQPQNLL VTANDQPVAI AGVMGGAATE VDENTQNIVL ETALFDGVTI
360 370 380 390 400
RKSSKAINLR SESSTRYERG VNRCELEVAL HRAIALMTEL AGGTVVRQGK
410 420 430 440 450
ADQRQDRGEA IINLRLERLQ QLLGKVNTPT GIGNITAEDV ERILTDLGCG
460 470 480 490 500
LTRQSDSDTP VWAVTVPSYR QRDIEREIDL IEEVARLYGY DHFCEQLPSN
510 520 530 540 550
TIAGGLSPSY QAELALREAC RGVGLTEVVH YSLVKPHGSE VMLANPLFAE
560 570 580 590 600
YSALRTNLLD GLITAFANNQ AQNNGALNAF EVGRVFWQNE GDIGEADHLA
610 620 630 640 650
GICGGSQITE GTWPQGGKPQ PMSWYDAKGL LEAIFQRLGA TVTYSGDHQD
660 670 680 690 700
PRLHPGRTAL LSCNGTVLGR FGQLHPQLRR EKGLIDEVYA FEITLTPLYQ
710 720 730 740 750
AMETQILGTP DFRPYSPYPA VARDLALYAP LELTVAELTQ AMVKAGGDLL
760 770 780 790 800
EQVELFDEYR GQSVPAGQRS LAFSLAYRVG DRTLTDADVE PLHNQIREAL
810
TKQFAVSLRS
Length:810
Mass (Da):87,888
Last modified:February 1, 1997 - v1
Checksum:i4D14168774C12BE9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA18390.1.
PIRiS75931.

Genome annotation databases

EnsemblBacteriaiBAA18390; BAA18390; BAA18390.
KEGGisyy:SYNGTS_1815.
syz:MYO_118330.
PATRICi23840898. VBISynSp132158_2003.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA18390.1.
PIRiS75931.

3D structure databases

ProteinModelPortaliP74296.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP74296. 4 interactions.

Proteomic databases

PaxDbiP74296.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA18390; BAA18390; BAA18390.
KEGGisyy:SYNGTS_1815.
syz:MYO_118330.
PATRICi23840898. VBISynSp132158_2003.

Phylogenomic databases

eggNOGiCOG0072.
HOGENOMiHOG000292087.
InParanoidiP74296.
KOiK01890.
OMAiMKFSEQW.
OrthoDBiEOG6CCH1J.
PhylomeDBiP74296.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.56.20. 1 hit.
3.30.70.380. 1 hit.
3.50.40.10. 1 hit.
HAMAPiMF_00283. Phe_tRNA_synth_beta1.
InterProiIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR012340. NA-bd_OB-fold.
IPR004532. Phe-tRNA-ligase_IIc_bsu.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR005121. PheS_beta_Fdx_antiC-bd.
IPR002547. tRNA-bd_dom.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
PfamiPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
PF03147. FDX-ACB. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
SMARTiSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
SM00896. FDX-ACB. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54991. SSF54991. 1 hit.
SSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR00472. pheT_bact. 1 hit.
PROSITEiPS51483. B5. 1 hit.
PS51447. FDX_ACB. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.

Entry informationi

Entry nameiSYFB_SYNY3
AccessioniPrimary (citable) accession number: P74296
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: July 22, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.