Phenylalanine--tRNA ligase beta subunit - Synechocystis sp. (strain PCC 6803 / Kazusa)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase beta subunit
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase beta subunit
Short name=PheRS

Gene names

Name:	pheT
Ordered Locus Names:	sll1553

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechocystis ›

Protein attributes

Sequence length	810 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP-Rule MF_00283
Cofactor	Binds 2 magnesium ions per tetramer By similarity.
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily. Contains 1 B5 domain. Contains 1 FDX-ACB domain. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP tRNA processing Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 810

810

Phenylalanine--tRNA ligase beta subunit HAMAP-Rule MF_00283

PRO_0000126974

Regions

Domain

39 – 150

112

tRNA-binding

Domain

407 – 495

89

B5

Domain

716 – 809

94

FDX-ACB

Sites

Metal binding

473

1

Magnesium By similarity

Metal binding

479

1

Magnesium; via carbonyl oxygen By similarity

Metal binding

482

1

Magnesium By similarity

Metal binding

483

1

Magnesium By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P74296 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 4D14168774C12BE9
Show »

FASTA

810

87,888

        10         20         30         40         50         60 
MRISVNWLQS LVELNLSPEE LGELLTIAGL EVEEIEDRRS WAAGVVLGRV ISREKHPNAD 

        70         80         90        100        110        120 
KLSVCVVDIG TEEPSTIVCG AANVRADILV PVATLGSYLP KVDLKIKPAK LRGVKSSGMI 

       130        140        150        160        170        180 
CSLAELGLSK ESEGIHIFPD LDLPSGSPVG PLLGLDDVIL EISPTANRAD ALSMVGVARE 

       190        200        210        220        230        240 
VAALTGGKLS LPEIKAVSVS DQDLPISVTE PQACPTYVGT VIRGVKVGPS PDWLQQRLLA 

       250        260        270        280        290        300 
AGTRPINNVV DVTNYVLLEW GQPLHSFDQD KLQTLVGPEG FALGVRFAEE GEKLITLDDQ 

       310        320        330        340        350        360 
ERTLQPQNLL VTANDQPVAI AGVMGGAATE VDENTQNIVL ETALFDGVTI RKSSKAINLR 

       370        380        390        400        410        420 
SESSTRYERG VNRCELEVAL HRAIALMTEL AGGTVVRQGK ADQRQDRGEA IINLRLERLQ 

       430        440        450        460        470        480 
QLLGKVNTPT GIGNITAEDV ERILTDLGCG LTRQSDSDTP VWAVTVPSYR QRDIEREIDL 

       490        500        510        520        530        540 
IEEVARLYGY DHFCEQLPSN TIAGGLSPSY QAELALREAC RGVGLTEVVH YSLVKPHGSE 

       550        560        570        580        590        600 
VMLANPLFAE YSALRTNLLD GLITAFANNQ AQNNGALNAF EVGRVFWQNE GDIGEADHLA 

       610        620        630        640        650        660 
GICGGSQITE GTWPQGGKPQ PMSWYDAKGL LEAIFQRLGA TVTYSGDHQD PRLHPGRTAL 

       670        680        690        700        710        720 
LSCNGTVLGR FGQLHPQLRR EKGLIDEVYA FEITLTPLYQ AMETQILGTP DFRPYSPYPA 

       730        740        750        760        770        780 
VARDLALYAP LELTVAELTQ AMVKAGGDLL EQVELFDEYR GQSVPAGQRS LAFSLAYRVG 

       790        800        810 
DRTLTDADVE PLHNQIREAL TKQFAVSLRS

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References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.

Tabata S.
DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA18390.1.
PIR	S75931.
RefSeq	NP_441710.1. NC_000911.1. YP_005651768.1. NC_017277.1. YP_007451592.1. NC_020286.1.
3D structure databases
ProteinModelPortal	P74296.
ModBase	Search...
Protein-protein interaction databases
IntAct	P74296. 4 interactions.
STRING	1148.sll1553.
Proteomic databases
PaxDb	P74296.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAA18390; BAA18390; BAA18390.
GeneID	12254423. 14617255. 952901.
KEGG	syn:sll1553. syy:SYNGTS_1815.
PATRIC	23840898. VBISynSp132158_2003.
Phylogenomic databases
eggNOG	COG0072.
HOGENOM	HOG000292087.
KO	K01890.
OMA	MKFSEQW.
ProtClustDB	PRK00629.
Family and domain databases
Gene3D	2.40.50.140. 1 hit. 3.30.56.20. 1 hit. 3.30.70.380. 1 hit. 3.50.40.10. 1 hit.
HAMAP	MF_00283. Phe_tRNA_synth_beta1.
InterPro	IPR005146. B3/B4_tRNA-bd. IPR009061. DNA-bd_dom_put. IPR012340. NA-bd_OB-fold. IPR004532. Phe-tRNA-ligase_IIc_bsu_bac. IPR020825. Phe-tRNA_synthase_B3/B4. IPR005121. PheS_beta_Fdx_antiC-bd. IPR002547. tRNA-bd_dom. IPR005147. tRNA_synthase_B5-dom. [Graphical view]
Pfam	PF03483. B3_4. 1 hit. PF03484. B5. 1 hit. PF03147. FDX-ACB. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
SMART	SM00873. B3_4. 1 hit. SM00874. B5. 1 hit. SM00896. FDX-ACB. 1 hit. [Graphical view]
SUPFAM	SSF56037. B3_4. 1 hit. SSF54991. Fdx_AntiC_bd. 1 hit. SSF50249. Nucleic_acid_OB. 1 hit. SSF46955. Putativ_DNA_bind. 1 hit.
TIGRFAMs	TIGR00472. pheT_bact. 1 hit.
PROSITE	PS51483. B5. 1 hit. PS51447. FDX_ACB. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFB_SYNY3

Accession

Primary (citable) accession number: P74296

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

Synechocystis PCC 6803

Synechocystis (strain PCC 6803): entries and gene names

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents