SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P74296

- SYFB_SYNY3

UniProt

P74296 - SYFB_SYNY3

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Phenylalanine--tRNA ligase beta subunit
Gene
pheT, sll1553
Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).UniRule annotation

Cofactori

Binds 2 magnesium ions per tetramer By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi473 – 4731Magnesium By similarity
Metal bindingi479 – 4791Magnesium; via carbonyl oxygen By similarity
Metal bindingi482 – 4821Magnesium By similarity
Metal bindingi483 – 4831Magnesium By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. phenylalanine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. phenylalanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
  2. tRNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylalanine--tRNA ligase beta subunit (EC:6.1.1.20)
Alternative name(s):
Phenylalanyl-tRNA synthetase beta subunit
Short name:
PheRS
Gene namesi
Name:pheT
Ordered Locus Names:sll1553
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 810810Phenylalanine--tRNA ligase beta subunitUniRule annotation
PRO_0000126974Add
BLAST

Proteomic databases

PaxDbiP74296.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits By similarity.

Protein-protein interaction databases

IntActiP74296. 4 interactions.
STRINGi1148.sll1553.

Structurei

3D structure databases

ProteinModelPortaliP74296.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 150112tRNA-binding
Add
BLAST
Domaini407 – 49589B5
Add
BLAST
Domaini716 – 80994FDX-ACB
Add
BLAST

Sequence similaritiesi

Contains 1 B5 domain.
Contains 1 FDX-ACB domain.

Phylogenomic databases

eggNOGiCOG0072.
HOGENOMiHOG000292087.
KOiK01890.
OMAiAISSTFC.
OrthoDBiEOG6CCH1J.
PhylomeDBiP74296.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.56.20. 1 hit.
3.30.70.380. 1 hit.
3.50.40.10. 1 hit.
HAMAPiMF_00283. Phe_tRNA_synth_beta1.
InterProiIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR012340. NA-bd_OB-fold.
IPR004532. Phe-tRNA-ligase_IIc_bsu.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR005121. PheS_beta_Fdx_antiC-bd.
IPR002547. tRNA-bd_dom.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
PfamiPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
PF03147. FDX-ACB. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
SMARTiSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
SM00896. FDX-ACB. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54991. SSF54991. 1 hit.
SSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR00472. pheT_bact. 1 hit.
PROSITEiPS51483. B5. 1 hit.
PS51447. FDX_ACB. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P74296-1 [UniParc]FASTAAdd to Basket

« Hide

MRISVNWLQS LVELNLSPEE LGELLTIAGL EVEEIEDRRS WAAGVVLGRV    50
ISREKHPNAD KLSVCVVDIG TEEPSTIVCG AANVRADILV PVATLGSYLP 100
KVDLKIKPAK LRGVKSSGMI CSLAELGLSK ESEGIHIFPD LDLPSGSPVG 150
PLLGLDDVIL EISPTANRAD ALSMVGVARE VAALTGGKLS LPEIKAVSVS 200
DQDLPISVTE PQACPTYVGT VIRGVKVGPS PDWLQQRLLA AGTRPINNVV 250
DVTNYVLLEW GQPLHSFDQD KLQTLVGPEG FALGVRFAEE GEKLITLDDQ 300
ERTLQPQNLL VTANDQPVAI AGVMGGAATE VDENTQNIVL ETALFDGVTI 350
RKSSKAINLR SESSTRYERG VNRCELEVAL HRAIALMTEL AGGTVVRQGK 400
ADQRQDRGEA IINLRLERLQ QLLGKVNTPT GIGNITAEDV ERILTDLGCG 450
LTRQSDSDTP VWAVTVPSYR QRDIEREIDL IEEVARLYGY DHFCEQLPSN 500
TIAGGLSPSY QAELALREAC RGVGLTEVVH YSLVKPHGSE VMLANPLFAE 550
YSALRTNLLD GLITAFANNQ AQNNGALNAF EVGRVFWQNE GDIGEADHLA 600
GICGGSQITE GTWPQGGKPQ PMSWYDAKGL LEAIFQRLGA TVTYSGDHQD 650
PRLHPGRTAL LSCNGTVLGR FGQLHPQLRR EKGLIDEVYA FEITLTPLYQ 700
AMETQILGTP DFRPYSPYPA VARDLALYAP LELTVAELTQ AMVKAGGDLL 750
EQVELFDEYR GQSVPAGQRS LAFSLAYRVG DRTLTDADVE PLHNQIREAL 800
TKQFAVSLRS 810
Length:810
Mass (Da):87,888
Last modified:February 1, 1997 - v1
Checksum:i4D14168774C12BE9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000022 Genomic DNA. Translation: BAA18390.1.
PIRiS75931.
RefSeqiNP_441710.1. NC_000911.1.
YP_005651768.1. NC_017277.1.
YP_007451592.1. NC_020286.1.

Genome annotation databases

EnsemblBacteriaiBAA18390; BAA18390; BAA18390.
GeneIDi952901.
KEGGisyn:sll1553.
syy:SYNGTS_1815.
syz:MYO_118330.
PATRICi23840898. VBISynSp132158_2003.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000022 Genomic DNA. Translation: BAA18390.1 .
PIRi S75931.
RefSeqi NP_441710.1. NC_000911.1.
YP_005651768.1. NC_017277.1.
YP_007451592.1. NC_020286.1.

3D structure databases

ProteinModelPortali P74296.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P74296. 4 interactions.
STRINGi 1148.sll1553.

Proteomic databases

PaxDbi P74296.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA18390 ; BAA18390 ; BAA18390 .
GeneIDi 952901.
KEGGi syn:sll1553.
syy:SYNGTS_1815.
syz:MYO_118330.
PATRICi 23840898. VBISynSp132158_2003.

Phylogenomic databases

eggNOGi COG0072.
HOGENOMi HOG000292087.
KOi K01890.
OMAi AISSTFC.
OrthoDBi EOG6CCH1J.
PhylomeDBi P74296.

Family and domain databases

Gene3Di 2.40.50.140. 1 hit.
3.30.56.20. 1 hit.
3.30.70.380. 1 hit.
3.50.40.10. 1 hit.
HAMAPi MF_00283. Phe_tRNA_synth_beta1.
InterProi IPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR012340. NA-bd_OB-fold.
IPR004532. Phe-tRNA-ligase_IIc_bsu.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR005121. PheS_beta_Fdx_antiC-bd.
IPR002547. tRNA-bd_dom.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view ]
Pfami PF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
PF03147. FDX-ACB. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view ]
SMARTi SM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
SM00896. FDX-ACB. 1 hit.
[Graphical view ]
SUPFAMi SSF46955. SSF46955. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54991. SSF54991. 1 hit.
SSF56037. SSF56037. 1 hit.
TIGRFAMsi TIGR00472. pheT_bact. 1 hit.
PROSITEi PS51483. B5. 1 hit.
PS51447. FDX_ACB. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.

Entry informationi

Entry nameiSYFB_SYNY3
AccessioniPrimary (citable) accession number: P74296
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: July 9, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3

Similar proteinsi