Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P74296

- SYFB_SYNY3

UniProt

P74296 - SYFB_SYNY3

Protein

Phenylalanine--tRNA ligase beta subunit

Gene

pheT

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).

    Cofactori

    Binds 2 magnesium ions per tetramer.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi473 – 4731MagnesiumBy similarity
    Metal bindingi479 – 4791Magnesium; via carbonyl oxygenBy similarity
    Metal bindingi482 – 4821MagnesiumBy similarity
    Metal bindingi483 – 4831MagnesiumBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. phenylalanine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. tRNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. phenylalanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
    2. tRNA processing Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phenylalanine--tRNA ligase beta subunit (EC:6.1.1.20)
    Alternative name(s):
    Phenylalanyl-tRNA synthetase beta subunit
    Short name:
    PheRS
    Gene namesi
    Name:pheT
    Ordered Locus Names:sll1553
    OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
    Taxonomic identifieri1111708 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
    ProteomesiUP000001425: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 810810Phenylalanine--tRNA ligase beta subunitPRO_0000126974Add
    BLAST

    Proteomic databases

    PaxDbiP74296.

    Interactioni

    Subunit structurei

    Tetramer of two alpha and two beta subunits.By similarity

    Protein-protein interaction databases

    IntActiP74296. 4 interactions.
    STRINGi1148.sll1553.

    Structurei

    3D structure databases

    ProteinModelPortaliP74296.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini39 – 150112tRNA-bindingAdd
    BLAST
    Domaini407 – 49589B5Add
    BLAST
    Domaini716 – 80994FDX-ACBAdd
    BLAST

    Sequence similaritiesi

    Contains 1 B5 domain.Curated
    Contains 1 FDX-ACB domain.Curated
    Contains 1 tRNA-binding domain.Curated

    Phylogenomic databases

    eggNOGiCOG0072.
    HOGENOMiHOG000292087.
    KOiK01890.
    OMAiAISSTFC.
    OrthoDBiEOG6CCH1J.
    PhylomeDBiP74296.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    3.30.56.20. 1 hit.
    3.30.70.380. 1 hit.
    3.50.40.10. 1 hit.
    HAMAPiMF_00283. Phe_tRNA_synth_beta1.
    InterProiIPR005146. B3/B4_tRNA-bd.
    IPR009061. DNA-bd_dom_put.
    IPR012340. NA-bd_OB-fold.
    IPR004532. Phe-tRNA-ligase_IIc_bsu.
    IPR020825. Phe-tRNA_synthase_B3/B4.
    IPR005121. PheS_beta_Fdx_antiC-bd.
    IPR002547. tRNA-bd_dom.
    IPR005147. tRNA_synthase_B5-dom.
    [Graphical view]
    PfamiPF03483. B3_4. 1 hit.
    PF03484. B5. 1 hit.
    PF03147. FDX-ACB. 1 hit.
    PF01588. tRNA_bind. 1 hit.
    [Graphical view]
    SMARTiSM00873. B3_4. 1 hit.
    SM00874. B5. 1 hit.
    SM00896. FDX-ACB. 1 hit.
    [Graphical view]
    SUPFAMiSSF46955. SSF46955. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54991. SSF54991. 1 hit.
    SSF56037. SSF56037. 1 hit.
    TIGRFAMsiTIGR00472. pheT_bact. 1 hit.
    PROSITEiPS51483. B5. 1 hit.
    PS51447. FDX_ACB. 1 hit.
    PS50886. TRBD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P74296-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRISVNWLQS LVELNLSPEE LGELLTIAGL EVEEIEDRRS WAAGVVLGRV    50
    ISREKHPNAD KLSVCVVDIG TEEPSTIVCG AANVRADILV PVATLGSYLP 100
    KVDLKIKPAK LRGVKSSGMI CSLAELGLSK ESEGIHIFPD LDLPSGSPVG 150
    PLLGLDDVIL EISPTANRAD ALSMVGVARE VAALTGGKLS LPEIKAVSVS 200
    DQDLPISVTE PQACPTYVGT VIRGVKVGPS PDWLQQRLLA AGTRPINNVV 250
    DVTNYVLLEW GQPLHSFDQD KLQTLVGPEG FALGVRFAEE GEKLITLDDQ 300
    ERTLQPQNLL VTANDQPVAI AGVMGGAATE VDENTQNIVL ETALFDGVTI 350
    RKSSKAINLR SESSTRYERG VNRCELEVAL HRAIALMTEL AGGTVVRQGK 400
    ADQRQDRGEA IINLRLERLQ QLLGKVNTPT GIGNITAEDV ERILTDLGCG 450
    LTRQSDSDTP VWAVTVPSYR QRDIEREIDL IEEVARLYGY DHFCEQLPSN 500
    TIAGGLSPSY QAELALREAC RGVGLTEVVH YSLVKPHGSE VMLANPLFAE 550
    YSALRTNLLD GLITAFANNQ AQNNGALNAF EVGRVFWQNE GDIGEADHLA 600
    GICGGSQITE GTWPQGGKPQ PMSWYDAKGL LEAIFQRLGA TVTYSGDHQD 650
    PRLHPGRTAL LSCNGTVLGR FGQLHPQLRR EKGLIDEVYA FEITLTPLYQ 700
    AMETQILGTP DFRPYSPYPA VARDLALYAP LELTVAELTQ AMVKAGGDLL 750
    EQVELFDEYR GQSVPAGQRS LAFSLAYRVG DRTLTDADVE PLHNQIREAL 800
    TKQFAVSLRS 810
    Length:810
    Mass (Da):87,888
    Last modified:February 1, 1997 - v1
    Checksum:i4D14168774C12BE9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000022 Genomic DNA. Translation: BAA18390.1.
    PIRiS75931.
    RefSeqiNP_441710.1. NC_000911.1.
    YP_005651768.1. NC_017277.1.
    YP_007451592.1. NC_020286.1.

    Genome annotation databases

    EnsemblBacteriaiBAA18390; BAA18390; BAA18390.
    GeneIDi952901.
    KEGGisyn:sll1553.
    syy:SYNGTS_1815.
    syz:MYO_118330.
    PATRICi23840898. VBISynSp132158_2003.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000022 Genomic DNA. Translation: BAA18390.1 .
    PIRi S75931.
    RefSeqi NP_441710.1. NC_000911.1.
    YP_005651768.1. NC_017277.1.
    YP_007451592.1. NC_020286.1.

    3D structure databases

    ProteinModelPortali P74296.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P74296. 4 interactions.
    STRINGi 1148.sll1553.

    Proteomic databases

    PaxDbi P74296.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA18390 ; BAA18390 ; BAA18390 .
    GeneIDi 952901.
    KEGGi syn:sll1553.
    syy:SYNGTS_1815.
    syz:MYO_118330.
    PATRICi 23840898. VBISynSp132158_2003.

    Phylogenomic databases

    eggNOGi COG0072.
    HOGENOMi HOG000292087.
    KOi K01890.
    OMAi AISSTFC.
    OrthoDBi EOG6CCH1J.
    PhylomeDBi P74296.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    3.30.56.20. 1 hit.
    3.30.70.380. 1 hit.
    3.50.40.10. 1 hit.
    HAMAPi MF_00283. Phe_tRNA_synth_beta1.
    InterProi IPR005146. B3/B4_tRNA-bd.
    IPR009061. DNA-bd_dom_put.
    IPR012340. NA-bd_OB-fold.
    IPR004532. Phe-tRNA-ligase_IIc_bsu.
    IPR020825. Phe-tRNA_synthase_B3/B4.
    IPR005121. PheS_beta_Fdx_antiC-bd.
    IPR002547. tRNA-bd_dom.
    IPR005147. tRNA_synthase_B5-dom.
    [Graphical view ]
    Pfami PF03483. B3_4. 1 hit.
    PF03484. B5. 1 hit.
    PF03147. FDX-ACB. 1 hit.
    PF01588. tRNA_bind. 1 hit.
    [Graphical view ]
    SMARTi SM00873. B3_4. 1 hit.
    SM00874. B5. 1 hit.
    SM00896. FDX-ACB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46955. SSF46955. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54991. SSF54991. 1 hit.
    SSF56037. SSF56037. 1 hit.
    TIGRFAMsi TIGR00472. pheT_bact. 1 hit.
    PROSITEi PS51483. B5. 1 hit.
    PS51447. FDX_ACB. 1 hit.
    PS50886. TRBD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
      Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
      , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PCC 6803 / Kazusa.

    Entry informationi

    Entry nameiSYFB_SYNY3
    AccessioniPrimary (citable) accession number: P74296
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Synechocystis PCC 6803
      Synechocystis (strain PCC 6803): entries and gene names

    External Data

    Dasty 3