UniProtKB - P74296 (SYFB_SYNY3)
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Protein
Phenylalanine--tRNA ligase beta subunit
Gene
pheT
Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Functioni
Catalytic activityi
ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).
Cofactori
Mg2+By similarityNote: Binds 2 magnesium ions per tetramer.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 473 | MagnesiumBy similarity | 1 | |
Metal bindingi | 479 | Magnesium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 482 | MagnesiumBy similarity | 1 | |
Metal bindingi | 483 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- magnesium ion binding Source: InterPro
- phenylalanine-tRNA ligase activity Source: UniProtKB-EC
- tRNA binding Source: UniProtKB-KW
GO - Biological processi
- phenylalanyl-tRNA aminoacylation Source: GO_Central
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase, RNA-binding, tRNA-binding |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Phenylalanine--tRNA ligase beta subunit (EC:6.1.1.20)Alternative name(s): Phenylalanyl-tRNA synthetase beta subunit Short name: PheRS |
Gene namesi | Name:pheT Ordered Locus Names:sll1553 |
Organismi | Synechocystis sp. (strain PCC 6803 / Kazusa) |
Taxonomic identifieri | 1111708 [NCBI] |
Taxonomic lineagei | Bacteria › Cyanobacteria › Synechococcales › Merismopediaceae › Synechocystis › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm By similarity
GO - Cellular componenti
- phenylalanine-tRNA ligase complex Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000126974 | 1 – 810 | Phenylalanine--tRNA ligase beta subunitAdd BLAST | 810 |
Proteomic databases
PaxDbi | P74296. |
Interactioni
Subunit structurei
Tetramer of two alpha and two beta subunits.By similarity
Protein-protein interaction databases
IntActi | P74296. 4 interactors. |
STRINGi | 1148.SYNGTS_1815. |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 39 – 150 | tRNA-bindingAdd BLAST | 112 | |
Domaini | 407 – 495 | B5Add BLAST | 89 | |
Domaini | 716 – 809 | FDX-ACBAdd BLAST | 94 |
Sequence similaritiesi
Phylogenomic databases
HOGENOMi | HOG000292087. |
InParanoidi | P74296. |
KOi | K01890. |
OMAi | LTPNRSD. |
PhylomeDBi | P74296. |
Family and domain databases
CDDi | cd02796. tRNA_bind_bactPheRS. 1 hit. |
Gene3Di | 3.30.70.380. 1 hit. 3.50.40.10. 1 hit. |
HAMAPi | MF_00283. Phe_tRNA_synth_beta1. 1 hit. |
InterProi | View protein in InterPro IPR005146. B3/B4_tRNA-bd. IPR009061. DNA-bd_dom_put_sf. IPR005121. Fdx_antiC-bd. IPR036690. Fdx_antiC-bd_sf. IPR012340. NA-bd_OB-fold. IPR004532. Phe-tRNA-ligase_IIc_bsu. IPR020825. Phe-tRNA_synthase_B3/B4. IPR002547. tRNA-bd_dom. IPR033714. tRNA_bind_bactPheRS. IPR005147. tRNA_synthase_B5-dom. |
Pfami | View protein in Pfam PF03483. B3_4. 1 hit. PF03484. B5. 1 hit. PF03147. FDX-ACB. 1 hit. PF01588. tRNA_bind. 1 hit. |
SMARTi | View protein in SMART SM00873. B3_4. 1 hit. SM00874. B5. 1 hit. SM00896. FDX-ACB. 1 hit. |
SUPFAMi | SSF46955. SSF46955. 1 hit. SSF50249. SSF50249. 1 hit. SSF54991. SSF54991. 1 hit. |
TIGRFAMsi | TIGR00472. pheT_bact. 1 hit. |
PROSITEi | View protein in PROSITE PS51483. B5. 1 hit. PS51447. FDX_ACB. 1 hit. PS50886. TRBD. 1 hit. |
i Sequence
Sequence statusi: Complete.
P74296-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRISVNWLQS LVELNLSPEE LGELLTIAGL EVEEIEDRRS WAAGVVLGRV
60 70 80 90 100
ISREKHPNAD KLSVCVVDIG TEEPSTIVCG AANVRADILV PVATLGSYLP
110 120 130 140 150
KVDLKIKPAK LRGVKSSGMI CSLAELGLSK ESEGIHIFPD LDLPSGSPVG
160 170 180 190 200
PLLGLDDVIL EISPTANRAD ALSMVGVARE VAALTGGKLS LPEIKAVSVS
210 220 230 240 250
DQDLPISVTE PQACPTYVGT VIRGVKVGPS PDWLQQRLLA AGTRPINNVV
260 270 280 290 300
DVTNYVLLEW GQPLHSFDQD KLQTLVGPEG FALGVRFAEE GEKLITLDDQ
310 320 330 340 350
ERTLQPQNLL VTANDQPVAI AGVMGGAATE VDENTQNIVL ETALFDGVTI
360 370 380 390 400
RKSSKAINLR SESSTRYERG VNRCELEVAL HRAIALMTEL AGGTVVRQGK
410 420 430 440 450
ADQRQDRGEA IINLRLERLQ QLLGKVNTPT GIGNITAEDV ERILTDLGCG
460 470 480 490 500
LTRQSDSDTP VWAVTVPSYR QRDIEREIDL IEEVARLYGY DHFCEQLPSN
510 520 530 540 550
TIAGGLSPSY QAELALREAC RGVGLTEVVH YSLVKPHGSE VMLANPLFAE
560 570 580 590 600
YSALRTNLLD GLITAFANNQ AQNNGALNAF EVGRVFWQNE GDIGEADHLA
610 620 630 640 650
GICGGSQITE GTWPQGGKPQ PMSWYDAKGL LEAIFQRLGA TVTYSGDHQD
660 670 680 690 700
PRLHPGRTAL LSCNGTVLGR FGQLHPQLRR EKGLIDEVYA FEITLTPLYQ
710 720 730 740 750
AMETQILGTP DFRPYSPYPA VARDLALYAP LELTVAELTQ AMVKAGGDLL
760 770 780 790 800
EQVELFDEYR GQSVPAGQRS LAFSLAYRVG DRTLTDADVE PLHNQIREAL
810
TKQFAVSLRS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BA000022 Genomic DNA. Translation: BAA18390.1. |
PIRi | S75931. |
Genome annotation databases
EnsemblBacteriai | BAA18390; BAA18390; BAA18390. |
KEGGi | syn:sll1553. |
Similar proteinsi
Entry informationi
Entry namei | SYFB_SYNY3 | |
Accessioni | P74296Primary (citable) accession number: P74296 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | February 1, 1997 | |
Last modified: | February 28, 2018 | |
This is version 127 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |