P74290 (FPG_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formamidopyrimidine-DNA glycosylase
Short name=Fapy-DNA glycosylase
EC=3.2.2.23

Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyase MutM
Short name=AP lyase MutM
EC=4.2.99.18

Gene names

Name:	mutM
Synonyms:	fpg
Ordered Locus Names:	slr1689

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechocystis › Synechocystis sp. PCC 6803

Protein attributes

Sequence length	287 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates By similarity. HAMAP-Rule MF_00103
Catalytic activity	Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine. HAMAP-Rule MF_00103 The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. HAMAP-Rule MF_00103
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00103
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00103
Sequence similarities	Belongs to the FPG family. Contains 1 FPG-type zinc finger.
Sequence caution	The sequence AAA88630.1 differs from that shown. Reason: Frameshift at position 38.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Glycosidase Hydrolase Lyase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	base-excision repair Inferred from electronic annotation. Source: InterPro nucleotide-excision repair Inferred from electronic annotation. Source: InterPro
Molecular_function	damaged DNA binding Inferred from electronic annotation. Source: InterPro oxidized purine nucleobase lesion DNA N-glycosylase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 287	286	Formamidopyrimidine-DNA glycosylase HAMAP-Rule MF_00103		PRO_0000170879
Regions
	Zinc finger	249 – 283	35	FPG-type HAMAP-Rule MF_00103
Sites
	Active site	2	1	Schiff-base intermediate with DNA By similarity
	Active site	3	1	Proton donor By similarity
	Active site	60	1	Proton donor; for beta-elimination activity By similarity
	Active site	273	1	Proton donor; for delta-elimination activity By similarity
	Binding site	100	1	DNA By similarity
	Binding site	119	1	DNA By similarity
Experimental info
	Sequence conflict	129 – 130	2	GD → AI in AAA88630. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P74290 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: FB9B4FE65916CC08	FASTA	287	32,062
	10 20 30 40 50 60 MPELPEVETV CRGLNRLTLE QTIGGGEVLL DRSLAYPVSV EDFQKQITGC RLVGWQRRGK 70 80 90 100 110 120 YLLGELRSNQ APGPAGWLGC HLRMTGQLLW TERDQQRPRH TRVVLHFEGG WELRFVDTRT 130 140 150 160 170 180 FGKVWLLPGD RPWAEVMTGL GQLGPEPFGA DFTAEYLYEK LKSSRRPLKN ALLDQRLVAG 190 200 210 220 230 240 LGNIYADEVL FFCGFHPTMA SNQVSLQDCE LIHQQIQATL TAAIEAGGTS FSDYRQVTGI 250 260 270 280 NGNYGGMAQV YGREGEPCRH CGTVIAKIKL GGRSAHFCPQ CQPKLER

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 6803 / Kazusa.
[2]	"Structure and targeted mutagenesis of the gene encoding 8-kDa subunit of photosystem I from the cyanobacterium Synechocystis sp. PCC 6803." Chitnis P.R., Reilly P.A., Miedel M.C., Nelson N. J. Biol. Chem. 264:18374-18380(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-169.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA18384.1. J05079 Genomic DNA. Translation: AAA88630.1. Frameshift.
PIR	S75925.
RefSeq	NP_441704.1. NC_000911.1. YP_005651762.1. NC_017277.1. YP_007451586.1. NC_020286.1.
3D structure databases
ProteinModelPortal	P74290.
ModBase	Search...
Protein-protein interaction databases
STRING	1148.slr1689.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	12254417. 14617249. 953005.
KEGG	syn:slr1689. syy:SYNGTS_1809.
PATRIC	23840886. VBISynSp132158_1997.
Phylogenomic databases
eggNOG	COG0266.
HOGENOM	HOG000020885.
KO	K10563.
OMA	VLRYNDP.
Family and domain databases
HAMAP	MF_00103. Fapy-DNA_glycosyl.
InterPro	IPR015886. DNA_glyclase/AP_lyase_DNA-bd. IPR015887. DNA_glyclase_Znf_dom_DNA_BS. IPR000191. DNA_glycosylase/AP_lyase. IPR012319. DNA_glycosylase/AP_lyase_cat. IPR020629. Formamido-pyr_DNA_Glyclase. IPR010979. Ribosomal_S13-like_H2TH. IPR000214. Znf_DNA_glyclase/AP_lyase. IPR010663. Znf_DNA_glyclase/IsotRNA_synth. [Graphical view]
Pfam	PF01149. Fapy_DNA_glyco. 1 hit. PF06831. H2TH. 1 hit. PF06827. zf-FPG_IleRS. 1 hit. [Graphical view]
SMART	SM00898. Fapy_DNA_glyco. 1 hit. [Graphical view]
SUPFAM	SSF81624. Form_DNAglyc_cat. 1 hit. SSF46946. Ribosomal_H2TH. 1 hit.
TIGRFAMs	TIGR00577. fpg. 1 hit.
PROSITE	PS51068. FPG_CAT. 1 hit. PS01242. ZF_FPG_1. 1 hit. PS51066. ZF_FPG_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FPG_SYNY3

Accession

Primary (citable) accession number: P74290
Secondary accession number(s): Q55328

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 102 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program