P74260 (GLPK_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol kinase
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	slr1672

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa)

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	495 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 495	495	Glycerol kinase HAMAP MF_00186		PRO_0000059511
Regions
	Nucleotide binding	417 – 421	5	ATP By similarity
Sites
	Binding site	16	1	Substrate By similarity
	Binding site	20	1	ATP By similarity
	Binding site	86	1	Substrate By similarity
	Binding site	138	1	Substrate By similarity
	Binding site	246	1	Substrate By similarity
	Binding site	268	1	ATP By similarity
	Binding site	316	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P74260 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 71AAC71E76816736	FASTA	495	54,351
	10 20 30 40 50 60 MTAKHNQYVM ALDLGTTGNR AILFDYEGNI VGQAYKELTQ FYPKAGWVEH DALEIWRDTK 70 80 90 100 110 120 TVMQEVVQKT AIQPQQIVAI GLTVQRETCL LWDKTTGQPL HPAIVWQDRR TAHFCGELTA 130 140 150 160 170 180 AGYVDEIYER TGLVLDAYFS GTKLHWLLDW VKQSKSVDPA NLLAGTIDSW ALWNLTGGKV 190 200 210 220 230 240 HRTDHSNASR TMVLNLDSLI WDEKLLDLFT IPAQIMPEVQ PSLSYFGVTD PEILGVEIPI 250 260 270 280 290 300 TAIFGDQQAA LYAHGCDRPG LLKCTYGTGA FLVANTGQTV TRSQHRLLST VAWTQTNRDK 310 320 330 340 350 360 SLTRDYALEG SMFTAGSCVQ WLRDKLGLIE SAAASESLAR SVDSNGGVYF VPALSGLGAP 370 380 390 400 410 420 HWDMNACGAF LGLTAGVTKA HLVRSVLEAI AFQAREVVEA INQDSPAPIQ QLKVDGGACN 430 440 450 460 470 480 NDFLMQCQAD VLGIPVERPA VLDATAQGAA FGAGLKIGWN QLQIRFFRTF HHGTISLICL 490 QFLPMRIQEG VSVDQ

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA18354.1.
PIR	S75895.
RefSeq	NP_441674.1. NC_000911.1.
3D structure databases
ProteinModelPortal	P74260.
ModBase	Search...
Protein-protein interaction databases
STRING	P74260.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	953024.
GenomeReviews	Gene locus slr1672 in contig BA000022_GR.
KEGG	syn:slr1672.
NMPDR	fig|1148.1.peg.1775.
PATRIC	23840819. VBISynSp132158_1964.
Phylogenomic databases
eggNOG	COG0554.
HOGENOM	HBG511469.
OMA	ATYFSGT.
PhylomeDB	P74260.
ProtClustDB	CLSK893231.
Enzyme and pathway databases
BioCyc	SSP1148:SLR1672-MONOMER.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
KO	K00864.
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. Glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. False negative. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_SYNY3

Accession

Primary (citable) accession number: P74260

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program