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Protein

Hydroperoxy fatty acid reductase gpx1

Gene

gpx1

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydroperoxy fatty acid reductase essential for the removal of lipid hydroperoxides under normal and stress conditions, leading to the protection of membrane integrity.1 Publication

Catalytic activityi

A hydroperoxy fatty acid + NADPH = a hydroxy fatty acid + NADP+ + H2O.1 Publication

Enzyme regulationi

Mercaptosuccinate, pCMB, and nethylmaleimide act as inhibitors of the catalytic activity.1 Publication

Kineticsi

  1. KM=83.1 µM for NADPH1 Publication
  2. KM=215 µM for alpha-linolenic acid hydroperoxide1 Publication

    pH dependencei

    Optimum pH is 8.2.1 Publication

    Temperature dependencei

    Optimum temperature is 37 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei41By similarity1

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase, Peroxidase

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-17842.

    Protein family/group databases

    PeroxiBasei3755. SYspGPx01.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hydroperoxy fatty acid reductase gpx1 (EC:1.11.1.22)
    Gene namesi
    Name:gpx1
    Ordered Locus Names:slr1171
    OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
    Taxonomic identifieri1111708 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesMerismopediaceaeSynechocystis
    Proteomesi
    • UP000001425 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000666631 – 169Hydroperoxy fatty acid reductase gpx1Add BLAST169

    Proteomic databases

    PRIDEiP74250.

    Expressioni

    Inductioni

    High light, methylviologen, t-butyl hydroperoxide, and salt stress conditions increase the expression level.1 Publication

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliP74250.
    SMRiP74250.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutathione peroxidase family.Curated

    Phylogenomic databases

    InParanoidiP74250.
    KOiK20207.
    OMAiTFYQLTA.
    PhylomeDBiP74250.

    Family and domain databases

    CDDicd00340. GSH_Peroxidase. 1 hit.
    Gene3Di3.40.30.10. 1 hit.
    InterProiIPR000889. Glutathione_peroxidase.
    IPR029759. GPX_AS.
    IPR029760. GPX_CS.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PANTHERiPTHR11592. PTHR11592. 1 hit.
    PfamiPF00255. GSHPx. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000303. Glutathion_perox. 1 hit.
    PRINTSiPR01011. GLUTPROXDASE.
    SUPFAMiSSF52833. SSF52833. 1 hit.
    PROSITEiPS00460. GLUTATHIONE_PEROXID_1. 1 hit.
    PS00763. GLUTATHIONE_PEROXID_2. 1 hit.
    PS51355. GLUTATHIONE_PEROXID_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P74250-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTAQANNTIY GFSANALDGS PVALRDFEGK VLLIVNTASQ CGFTPQYQGL
    60 70 80 90 100
    QALYNRFGDR GFTVLGFPCN QFGQQEPGGS GEIKNFCETR YGVTFPLFEK
    110 120 130 140 150
    VEVNGPNAHP LFKFLTAASP GMAIPFLGGA EDIKWNFTKF LVDRQGKVVK
    160
    RYGSIAKPDE IAADIEKLL
    Length:169
    Mass (Da):18,452
    Last modified:February 1, 1997 - v1
    Checksum:i0DC382089FCE39E2
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    BA000022 Genomic DNA. Translation: BAA18344.1.
    PIRiS75885.

    Genome annotation databases

    EnsemblBacteriaiBAA18344; BAA18344; BAA18344.
    KEGGisyn:slr1171.
    PATRICi23840787. VBISynSp132158_1948.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    BA000022 Genomic DNA. Translation: BAA18344.1.
    PIRiS75885.

    3D structure databases

    ProteinModelPortaliP74250.
    SMRiP74250.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein family/group databases

    PeroxiBasei3755. SYspGPx01.

    Proteomic databases

    PRIDEiP74250.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiBAA18344; BAA18344; BAA18344.
    KEGGisyn:slr1171.
    PATRICi23840787. VBISynSp132158_1948.

    Phylogenomic databases

    InParanoidiP74250.
    KOiK20207.
    OMAiTFYQLTA.
    PhylomeDBiP74250.

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-17842.

    Family and domain databases

    CDDicd00340. GSH_Peroxidase. 1 hit.
    Gene3Di3.40.30.10. 1 hit.
    InterProiIPR000889. Glutathione_peroxidase.
    IPR029759. GPX_AS.
    IPR029760. GPX_CS.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PANTHERiPTHR11592. PTHR11592. 1 hit.
    PfamiPF00255. GSHPx. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000303. Glutathion_perox. 1 hit.
    PRINTSiPR01011. GLUTPROXDASE.
    SUPFAMiSSF52833. SSF52833. 1 hit.
    PROSITEiPS00460. GLUTATHIONE_PEROXID_1. 1 hit.
    PS00763. GLUTATHIONE_PEROXID_2. 1 hit.
    PS51355. GLUTATHIONE_PEROXID_3. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiGPX1_SYNY3
    AccessioniPrimary (citable) accession number: P74250
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: February 1, 1997
    Last modified: November 2, 2016
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Synechocystis PCC 6803
      Synechocystis (strain PCC 6803): entries and gene names

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.