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Protein

Ribonucleoside-diphosphate reductase subunit alpha

Gene

nrdA

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction (By similarity).By similarity

Pathwayi: DNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei228SubstrateBy similarity1
Sitei244Important for hydrogen atom transferBy similarity1
Sitei251Allosteric effector bindingBy similarity1
Binding sitei272Substrate; via amide nitrogenBy similarity1
Sitei281Allosteric effector bindingBy similarity1
Active sitei460Proton acceptorBy similarity1
Active sitei462Cysteine radical intermediateBy similarity1
Active sitei464Proton acceptorBy similarity1
Sitei478Important for hydrogen atom transferBy similarity1
Sitei745Important for electron transferBy similarity1
Sitei746Important for electron transferBy similarity1
Sitei762Interacts with thioredoxin/glutaredoxinBy similarity1
Sitei765Interacts with thioredoxin/glutaredoxinBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase subunit alpha (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase
Gene namesi
Name:nrdA
Ordered Locus Names:slr1164
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesMerismopediaceaeSynechocystis
Proteomesi
  • UP000001425 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001872221 – 767Ribonucleoside-diphosphate reductase subunit alphaAdd BLAST767

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi244 ↔ 478Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP74240.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.By similarity

Protein-protein interaction databases

IntActiP74240. 3 interactors.

Structurei

3D structure databases

ProteinModelPortaliP74240.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 120ATP-conePROSITE-ProRule annotationAdd BLAST90

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni243 – 244Substrate bindingBy similarity2
Regioni460 – 464Substrate bindingBy similarity5
Regioni631 – 635Substrate bindingBy similarity5

Sequence similaritiesi

Contains 1 ATP-cone domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000057034.
InParanoidiP74240.
KOiK00525.
OMAiKLKCASI.
PhylomeDBiP74240.

Family and domain databases

InterProiIPR005144. ATP-cone_dom.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF03477. ATP-cone. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS51161. ATP_CONE. 1 hit.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P74240-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHPTLISAPI SSSANDAHAG TSQGSHQGHR IQVIRRDGSS TPLNIGKIRA
60 70 80 90 100
VVDWACLGLE VNSIALEAGL TTRLREGIST REIQDNLISC ALEMCSPNEP
110 120 130 140 150
DWRYVAGRLH VWSLWKDTLV RRGYQYGQYL RTVQTKVTNG EYDSRILTYS
160 170 180 190 200
EGELQEAGCW INSDWDTDYD YAGAVLLTSR YLLPNELPQE ALLTCALLLA
210 220 230 240 250
SVEAPDRRLQ WARRFYESIA ARRISLATPI LANLRVPGGS LTSCFIVAME
260 270 280 290 300
DNLESIFGEI TNAARISKNG GGVGVNVSRI RATGSWVMGK PNASGGVIPW
310 320 330 340 350
TKLLNDTAIA VNQGGRRAGA VTVGLDVWHL DVPEFLEMQA ENGDQRRKAY
360 370 380 390 400
DIFPQLILPD EFMRRVINKE DWTLVDPYEV REKMGIELAE LWGEQFEGAY
410 420 430 440 450
REIESNLDTT ITLYKRINAR ELFKQIMRTQ VETGMPYLSF KDTINKANPN
460 470 480 490 500
KHLGYIPGTN LCCESFSNVT PGQDAHCCNL VSLNLANLDL QDIAGVSQIA
510 520 530 540 550
VRMLDNTIEL TAPPFADAKS HNNKYRTIGV GAMGLADWLA KRRLNYDELA
560 570 580 590 600
DINRLFEEIG YWCTQSSMEL AKERGAYPAF PGSDWQKGLL IGSKPVSWFQ
610 620 630 640 650
ANAAKPERWE KLSNDIQTHG IRNSHITAIA PNTSSSLVQG CTASILPVYS
660 670 680 690 700
RFFYDKWAKG TVPIAPPFIG NCFWFYPENK TMDQRKVVKA VAAIQQWTDT
710 720 730 740 750
GISMELLFNL NAGIYFPEEP ERSLNAKDIF DTLVMAWEAG CKAIYYIRTV
760
QKDDFKDSSD GCVACAN
Length:767
Mass (Da):85,637
Last modified:February 1, 1997 - v1
Checksum:i147151C406A082C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA18334.1.
PIRiS75875.

Genome annotation databases

EnsemblBacteriaiBAA18334; BAA18334; BAA18334.
KEGGisyn:slr1164.
PATRICi23840765. VBISynSp132158_1937.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA18334.1.
PIRiS75875.

3D structure databases

ProteinModelPortaliP74240.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP74240. 3 interactors.

Proteomic databases

PRIDEiP74240.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA18334; BAA18334; BAA18334.
KEGGisyn:slr1164.
PATRICi23840765. VBISynSp132158_1937.

Phylogenomic databases

HOGENOMiHOG000057034.
InParanoidiP74240.
KOiK00525.
OMAiKLKCASI.
PhylomeDBiP74240.

Enzyme and pathway databases

UniPathwayiUPA00326.

Family and domain databases

InterProiIPR005144. ATP-cone_dom.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF03477. ATP-cone. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS51161. ATP_CONE. 1 hit.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIR1_SYNY3
AccessioniPrimary (citable) accession number: P74240
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.