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P74240

- RIR1_SYNY3

UniProt

P74240 - RIR1_SYNY3

Protein

Ribonucleoside-diphosphate reductase subunit alpha

Gene

nrdA

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.By similarity

    Catalytic activityi

    2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

    Enzyme regulationi

    Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei228 – 2281SubstrateBy similarity
    Sitei244 – 2441Important for hydrogen atom transferBy similarity
    Sitei251 – 2511Allosteric effector bindingBy similarity
    Binding sitei272 – 2721Substrate; via amide nitrogenBy similarity
    Sitei281 – 2811Allosteric effector bindingBy similarity
    Active sitei460 – 4601Proton acceptorBy similarity
    Active sitei462 – 4621Cysteine radical intermediateBy similarity
    Active sitei464 – 4641Proton acceptorBy similarity
    Sitei478 – 4781Important for hydrogen atom transferBy similarity
    Sitei745 – 7451Important for electron transferBy similarity
    Sitei746 – 7461Important for electron transferBy similarity
    Sitei762 – 7621Interacts with thioredoxin/glutaredoxinBy similarity
    Sitei765 – 7651Interacts with thioredoxin/glutaredoxinBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

    GO - Biological processi

    1. DNA replication Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00326.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonucleoside-diphosphate reductase subunit alpha (EC:1.17.4.1)
    Alternative name(s):
    Ribonucleotide reductase
    Gene namesi
    Name:nrdA
    Ordered Locus Names:slr1164
    OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
    Taxonomic identifieri1111708 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
    ProteomesiUP000001425: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. ribonucleoside-diphosphate reductase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 767767Ribonucleoside-diphosphate reductase subunit alphaPRO_0000187222Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi244 ↔ 478Redox-activeBy similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiP74240.

    Interactioni

    Subunit structurei

    Tetramer of two alpha and two beta subunits.By similarity

    Protein-protein interaction databases

    IntActiP74240. 3 interactions.
    STRINGi1148.slr1164.

    Structurei

    3D structure databases

    ProteinModelPortaliP74240.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini31 – 12090ATP-conePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni243 – 2442Substrate bindingBy similarity
    Regioni460 – 4645Substrate bindingBy similarity
    Regioni631 – 6355Substrate bindingBy similarity

    Sequence similaritiesi

    Contains 1 ATP-cone domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0209.
    HOGENOMiHOG000057034.
    KOiK00525.
    OMAiRDGQYES.
    OrthoDBiEOG6J48HC.
    PhylomeDBiP74240.

    Family and domain databases

    InterProiIPR005144. ATP-cone.
    IPR013346. NrdE_NrdA.
    IPR000788. RNR_lg_C.
    IPR013509. RNR_lsu_N.
    IPR008926. RNR_R1-su_N.
    [Graphical view]
    PfamiPF03477. ATP-cone. 1 hit.
    PF02867. Ribonuc_red_lgC. 1 hit.
    PF00317. Ribonuc_red_lgN. 1 hit.
    [Graphical view]
    PRINTSiPR01183. RIBORDTASEM1.
    SUPFAMiSSF48168. SSF48168. 1 hit.
    TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
    PROSITEiPS51161. ATP_CONE. 1 hit.
    PS00089. RIBORED_LARGE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P74240-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHPTLISAPI SSSANDAHAG TSQGSHQGHR IQVIRRDGSS TPLNIGKIRA    50
    VVDWACLGLE VNSIALEAGL TTRLREGIST REIQDNLISC ALEMCSPNEP 100
    DWRYVAGRLH VWSLWKDTLV RRGYQYGQYL RTVQTKVTNG EYDSRILTYS 150
    EGELQEAGCW INSDWDTDYD YAGAVLLTSR YLLPNELPQE ALLTCALLLA 200
    SVEAPDRRLQ WARRFYESIA ARRISLATPI LANLRVPGGS LTSCFIVAME 250
    DNLESIFGEI TNAARISKNG GGVGVNVSRI RATGSWVMGK PNASGGVIPW 300
    TKLLNDTAIA VNQGGRRAGA VTVGLDVWHL DVPEFLEMQA ENGDQRRKAY 350
    DIFPQLILPD EFMRRVINKE DWTLVDPYEV REKMGIELAE LWGEQFEGAY 400
    REIESNLDTT ITLYKRINAR ELFKQIMRTQ VETGMPYLSF KDTINKANPN 450
    KHLGYIPGTN LCCESFSNVT PGQDAHCCNL VSLNLANLDL QDIAGVSQIA 500
    VRMLDNTIEL TAPPFADAKS HNNKYRTIGV GAMGLADWLA KRRLNYDELA 550
    DINRLFEEIG YWCTQSSMEL AKERGAYPAF PGSDWQKGLL IGSKPVSWFQ 600
    ANAAKPERWE KLSNDIQTHG IRNSHITAIA PNTSSSLVQG CTASILPVYS 650
    RFFYDKWAKG TVPIAPPFIG NCFWFYPENK TMDQRKVVKA VAAIQQWTDT 700
    GISMELLFNL NAGIYFPEEP ERSLNAKDIF DTLVMAWEAG CKAIYYIRTV 750
    QKDDFKDSSD GCVACAN 767
    Length:767
    Mass (Da):85,637
    Last modified:February 1, 1997 - v1
    Checksum:i147151C406A082C5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000022 Genomic DNA. Translation: BAA18334.1.
    PIRiS75875.
    RefSeqiNP_441654.1. NC_000911.1.
    YP_005651712.1. NC_017277.1.
    YP_007451536.1. NC_020286.1.

    Genome annotation databases

    EnsemblBacteriaiBAA18334; BAA18334; BAA18334.
    GeneIDi954980.
    KEGGisyn:slr1164.
    syy:SYNGTS_1759.
    syz:MYO_117770.
    PATRICi23840765. VBISynSp132158_1937.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000022 Genomic DNA. Translation: BAA18334.1 .
    PIRi S75875.
    RefSeqi NP_441654.1. NC_000911.1.
    YP_005651712.1. NC_017277.1.
    YP_007451536.1. NC_020286.1.

    3D structure databases

    ProteinModelPortali P74240.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P74240. 3 interactions.
    STRINGi 1148.slr1164.

    Proteomic databases

    PaxDbi P74240.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA18334 ; BAA18334 ; BAA18334 .
    GeneIDi 954980.
    KEGGi syn:slr1164.
    syy:SYNGTS_1759.
    syz:MYO_117770.
    PATRICi 23840765. VBISynSp132158_1937.

    Phylogenomic databases

    eggNOGi COG0209.
    HOGENOMi HOG000057034.
    KOi K00525.
    OMAi RDGQYES.
    OrthoDBi EOG6J48HC.
    PhylomeDBi P74240.

    Enzyme and pathway databases

    UniPathwayi UPA00326 .

    Family and domain databases

    InterProi IPR005144. ATP-cone.
    IPR013346. NrdE_NrdA.
    IPR000788. RNR_lg_C.
    IPR013509. RNR_lsu_N.
    IPR008926. RNR_R1-su_N.
    [Graphical view ]
    Pfami PF03477. ATP-cone. 1 hit.
    PF02867. Ribonuc_red_lgC. 1 hit.
    PF00317. Ribonuc_red_lgN. 1 hit.
    [Graphical view ]
    PRINTSi PR01183. RIBORDTASEM1.
    SUPFAMi SSF48168. SSF48168. 1 hit.
    TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
    PROSITEi PS51161. ATP_CONE. 1 hit.
    PS00089. RIBORED_LARGE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
      Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
      , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PCC 6803 / Kazusa.

    Entry informationi

    Entry nameiRIR1_SYNY3
    AccessioniPrimary (citable) accession number: P74240
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Synechocystis PCC 6803
      Synechocystis (strain PCC 6803): entries and gene names

    External Data

    Dasty 3