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P74240 (RIR1_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonucleoside-diphosphate reductase subunit alpha

EC=1.17.4.1
Alternative name(s):
Ribonucleotide reductase
Gene names
Name:nrdA
Ordered Locus Names:slr1164
OrganismSynechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]
Taxonomic identifier1111708 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis

Protein attributes

Sequence length767 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activity

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulation

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction By similarity.

Pathway

Genetic information processing; DNA replication.

Subunit structure

Tetramer of two alpha and two beta subunits By similarity.

Sequence similarities

Belongs to the ribonucleoside diphosphate reductase large chain family.

Contains 1 ATP-cone domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 767767Ribonucleoside-diphosphate reductase subunit alpha
PRO_0000187222

Regions

Domain31 – 12090ATP-cone
Region243 – 2442Substrate binding By similarity
Region460 – 4645Substrate binding By similarity
Region631 – 6355Substrate binding By similarity

Sites

Active site4601Proton acceptor By similarity
Active site4621Cysteine radical intermediate By similarity
Active site4641Proton acceptor By similarity
Binding site2281Substrate By similarity
Binding site2721Substrate; via amide nitrogen By similarity
Site2441Important for hydrogen atom transfer By similarity
Site2511Allosteric effector binding By similarity
Site2811Allosteric effector binding By similarity
Site4781Important for hydrogen atom transfer By similarity
Site7451Important for electron transfer By similarity
Site7461Important for electron transfer By similarity
Site7621Interacts with thioredoxin/glutaredoxin By similarity
Site7651Interacts with thioredoxin/glutaredoxin By similarity

Amino acid modifications

Disulfide bond244 ↔ 478Redox-active By similarity

Sequences

Sequence LengthMass (Da)Tools
P74240 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 147151C406A082C5

FASTA76785,637
        10         20         30         40         50         60 
MHPTLISAPI SSSANDAHAG TSQGSHQGHR IQVIRRDGSS TPLNIGKIRA VVDWACLGLE 

        70         80         90        100        110        120 
VNSIALEAGL TTRLREGIST REIQDNLISC ALEMCSPNEP DWRYVAGRLH VWSLWKDTLV 

       130        140        150        160        170        180 
RRGYQYGQYL RTVQTKVTNG EYDSRILTYS EGELQEAGCW INSDWDTDYD YAGAVLLTSR 

       190        200        210        220        230        240 
YLLPNELPQE ALLTCALLLA SVEAPDRRLQ WARRFYESIA ARRISLATPI LANLRVPGGS 

       250        260        270        280        290        300 
LTSCFIVAME DNLESIFGEI TNAARISKNG GGVGVNVSRI RATGSWVMGK PNASGGVIPW 

       310        320        330        340        350        360 
TKLLNDTAIA VNQGGRRAGA VTVGLDVWHL DVPEFLEMQA ENGDQRRKAY DIFPQLILPD 

       370        380        390        400        410        420 
EFMRRVINKE DWTLVDPYEV REKMGIELAE LWGEQFEGAY REIESNLDTT ITLYKRINAR 

       430        440        450        460        470        480 
ELFKQIMRTQ VETGMPYLSF KDTINKANPN KHLGYIPGTN LCCESFSNVT PGQDAHCCNL 

       490        500        510        520        530        540 
VSLNLANLDL QDIAGVSQIA VRMLDNTIEL TAPPFADAKS HNNKYRTIGV GAMGLADWLA 

       550        560        570        580        590        600 
KRRLNYDELA DINRLFEEIG YWCTQSSMEL AKERGAYPAF PGSDWQKGLL IGSKPVSWFQ 

       610        620        630        640        650        660 
ANAAKPERWE KLSNDIQTHG IRNSHITAIA PNTSSSLVQG CTASILPVYS RFFYDKWAKG 

       670        680        690        700        710        720 
TVPIAPPFIG NCFWFYPENK TMDQRKVVKA VAAIQQWTDT GISMELLFNL NAGIYFPEEP 

       730        740        750        760 
ERSLNAKDIF DTLVMAWEAG CKAIYYIRTV QKDDFKDSSD GCVACAN 

« Hide

References

[1]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. expand/collapse author list , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000022 Genomic DNA. Translation: BAA18334.1.
PIRS75875.
RefSeqNP_441654.1. NC_000911.1.
YP_005651712.1. NC_017277.1.
YP_007451536.1. NC_020286.1.

3D structure databases

ProteinModelPortalP74240.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP74240. 3 interactions.
STRING1148.slr1164.

Proteomic databases

PaxDbP74240.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA18334; BAA18334; BAA18334.
GeneID954980.
KEGGsyn:slr1164.
syy:SYNGTS_1759.
syz:MYO_117770.
PATRIC23840765. VBISynSp132158_1937.

Phylogenomic databases

eggNOGCOG0209.
HOGENOMHOG000057034.
KOK00525.
OMARDGQYES.
OrthoDBEOG6J48HC.
PhylomeDBP74240.

Enzyme and pathway databases

UniPathwayUPA00326.

Family and domain databases

InterProIPR005144. ATP-cone.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamPF03477. ATP-cone. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSPR01183. RIBORDTASEM1.
SUPFAMSSF48168. SSF48168. 1 hit.
TIGRFAMsTIGR02506. NrdE_NrdA. 1 hit.
PROSITEPS51161. ATP_CONE. 1 hit.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRIR1_SYNY3
AccessionPrimary (citable) accession number: P74240
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 1, 1997
Last modified: July 9, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Synechocystis PCC 6803

Synechocystis (strain PCC 6803): entries and gene names

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways