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P74240

- RIR1_SYNY3

UniProt

P74240 - RIR1_SYNY3

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Protein

Ribonucleoside-diphosphate reductase subunit alpha

Gene

nrdA

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction (By similarity).By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei228 – 2281SubstrateBy similarity
Sitei244 – 2441Important for hydrogen atom transferBy similarity
Sitei251 – 2511Allosteric effector bindingBy similarity
Binding sitei272 – 2721Substrate; via amide nitrogenBy similarity
Sitei281 – 2811Allosteric effector bindingBy similarity
Active sitei460 – 4601Proton acceptorBy similarity
Active sitei462 – 4621Cysteine radical intermediateBy similarity
Active sitei464 – 4641Proton acceptorBy similarity
Sitei478 – 4781Important for hydrogen atom transferBy similarity
Sitei745 – 7451Important for electron transferBy similarity
Sitei746 – 7461Important for electron transferBy similarity
Sitei762 – 7621Interacts with thioredoxin/glutaredoxinBy similarity
Sitei765 – 7651Interacts with thioredoxin/glutaredoxinBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase subunit alpha (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase
Gene namesi
Name:nrdA
Ordered Locus Names:slr1164
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 767767Ribonucleoside-diphosphate reductase subunit alphaPRO_0000187222Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi244 ↔ 478Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP74240.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.By similarity

Protein-protein interaction databases

IntActiP74240. 3 interactions.
STRINGi1148.slr1164.

Structurei

3D structure databases

ProteinModelPortaliP74240.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 12090ATP-conePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni243 – 2442Substrate bindingBy similarity
Regioni460 – 4645Substrate bindingBy similarity
Regioni631 – 6355Substrate bindingBy similarity

Sequence similaritiesi

Contains 1 ATP-cone domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0209.
HOGENOMiHOG000057034.
InParanoidiP74240.
KOiK00525.
OMAiRDGQYES.
OrthoDBiEOG6J48HC.
PhylomeDBiP74240.

Family and domain databases

InterProiIPR005144. ATP-cone.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF03477. ATP-cone. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS51161. ATP_CONE. 1 hit.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P74240-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MHPTLISAPI SSSANDAHAG TSQGSHQGHR IQVIRRDGSS TPLNIGKIRA
60 70 80 90 100
VVDWACLGLE VNSIALEAGL TTRLREGIST REIQDNLISC ALEMCSPNEP
110 120 130 140 150
DWRYVAGRLH VWSLWKDTLV RRGYQYGQYL RTVQTKVTNG EYDSRILTYS
160 170 180 190 200
EGELQEAGCW INSDWDTDYD YAGAVLLTSR YLLPNELPQE ALLTCALLLA
210 220 230 240 250
SVEAPDRRLQ WARRFYESIA ARRISLATPI LANLRVPGGS LTSCFIVAME
260 270 280 290 300
DNLESIFGEI TNAARISKNG GGVGVNVSRI RATGSWVMGK PNASGGVIPW
310 320 330 340 350
TKLLNDTAIA VNQGGRRAGA VTVGLDVWHL DVPEFLEMQA ENGDQRRKAY
360 370 380 390 400
DIFPQLILPD EFMRRVINKE DWTLVDPYEV REKMGIELAE LWGEQFEGAY
410 420 430 440 450
REIESNLDTT ITLYKRINAR ELFKQIMRTQ VETGMPYLSF KDTINKANPN
460 470 480 490 500
KHLGYIPGTN LCCESFSNVT PGQDAHCCNL VSLNLANLDL QDIAGVSQIA
510 520 530 540 550
VRMLDNTIEL TAPPFADAKS HNNKYRTIGV GAMGLADWLA KRRLNYDELA
560 570 580 590 600
DINRLFEEIG YWCTQSSMEL AKERGAYPAF PGSDWQKGLL IGSKPVSWFQ
610 620 630 640 650
ANAAKPERWE KLSNDIQTHG IRNSHITAIA PNTSSSLVQG CTASILPVYS
660 670 680 690 700
RFFYDKWAKG TVPIAPPFIG NCFWFYPENK TMDQRKVVKA VAAIQQWTDT
710 720 730 740 750
GISMELLFNL NAGIYFPEEP ERSLNAKDIF DTLVMAWEAG CKAIYYIRTV
760
QKDDFKDSSD GCVACAN
Length:767
Mass (Da):85,637
Last modified:February 1, 1997 - v1
Checksum:i147151C406A082C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA18334.1.
PIRiS75875.
RefSeqiNP_441654.1. NC_000911.1.
YP_005651712.1. NC_017277.1.
YP_007451536.1. NC_020286.1.

Genome annotation databases

EnsemblBacteriaiBAA18334; BAA18334; BAA18334.
GeneIDi954980.
KEGGisyn:slr1164.
PATRICi23840765. VBISynSp132158_1937.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA18334.1 .
PIRi S75875.
RefSeqi NP_441654.1. NC_000911.1.
YP_005651712.1. NC_017277.1.
YP_007451536.1. NC_020286.1.

3D structure databases

ProteinModelPortali P74240.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P74240. 3 interactions.
STRINGi 1148.slr1164.

Proteomic databases

PaxDbi P74240.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA18334 ; BAA18334 ; BAA18334 .
GeneIDi 954980.
KEGGi syn:slr1164.
PATRICi 23840765. VBISynSp132158_1937.

Phylogenomic databases

eggNOGi COG0209.
HOGENOMi HOG000057034.
InParanoidi P74240.
KOi K00525.
OMAi RDGQYES.
OrthoDBi EOG6J48HC.
PhylomeDBi P74240.

Enzyme and pathway databases

UniPathwayi UPA00326 .

Family and domain databases

InterProi IPR005144. ATP-cone.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view ]
Pfami PF03477. ATP-cone. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
SUPFAMi SSF48168. SSF48168. 1 hit.
TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
PROSITEi PS51161. ATP_CONE. 1 hit.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.

Entry informationi

Entry nameiRIR1_SYNY3
AccessioniPrimary (citable) accession number: P74240
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 1, 1997
Last modified: November 26, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3