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P74232 (PUR2_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:slr1159
OrganismSynechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]
Taxonomic identifier1111708 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis

Protein attributes

Sequence length419 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 419419Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151494

Regions

Domain109 – 311203ATP-grasp
Nucleotide binding135 – 19157ATP By similarity

Sites

Metal binding2811Magnesium or manganese By similarity
Metal binding2831Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
P74232 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 9701E513A08CF2BC

FASTA41944,017
        10         20         30         40         50         60 
MKVAVIGSGG REHTLAWSLV RSPEVSHCYC LPGNGGTAGL PKTENVAIAV DQLGEICEFC 

        70         80         90        100        110        120 
QTEKINLVVV GPELPLTLGL TDQLQALGIK VFGPTKAGAE LEASKSWTKQ LLIEAGVPTA 

       130        140        150        160        170        180 
FGETFTEPAP AQAYATKMGA PIVVKADGLA AGKGVIVAQT SAEATTAIAE LFDQGFEKIV 

       190        200        210        220        230        240 
VEEFLPGEEV SVLALCDGKT VIPLLPAQDH KRIGEGDQGL NTGGMGAYCP APIAPPAVID 

       250        260        270        280        290        300 
QVQKQILQPT ANALAKRGID YRGVLYAGLM VSPTGEIKVL EYNCRFGDPE TQAVLPLLAT 

       310        320        330        340        350        360 
PLEKVLMACV EQNLEQLGPL QWHSGNAVCV VVAAGGYPGS YRKGDEINGL AEAEAQDVKV 

       370        380        390        400        410 
FHAGTELKEG KVLTNGGRVL GVTALGKDLS TAIATAYRGV EQIDFDGMYY RRDIGHKAL 

« Hide

References

[1]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. expand/collapse author list , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000022 Genomic DNA. Translation: BAA18326.1.
PIRS75867.
RefSeqNP_441646.1. NC_000911.1.
YP_005651704.1. NC_017277.1.
YP_007451528.1. NC_020286.1.

3D structure databases

ProteinModelPortalP74232.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING1148.slr1159.

Proteomic databases

PaxDbP74232.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA18326; BAA18326; BAA18326.
GeneID12254359.
14617191.
953042.
KEGGsyn:slr1159.
syy:SYNGTS_1751.
syz:MYO_117690.
PATRIC23840743. VBISynSp132158_1926.

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033463.
KOK01945.
OMADPEAMNV.
OrthoDBEOG69SKD1.
PhylomeDBP74232.
ProtClustDBPRK00885.

Enzyme and pathway databases

UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_SYNY3
AccessionPrimary (citable) accession number: P74232
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: April 16, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Synechocystis PCC 6803

Synechocystis (strain PCC 6803): entries and gene names

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways