P74227 (EFTU_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor Tu
Short name=EF-Tu

Gene names

Name:	tuf
Synonyms:	tufA
Ordered Locus Names:	sll1099

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa)

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	399 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. HAMAP MF_00118_B
Subunit structure	Monomer By similarity. HAMAP MF_00118_B
Subcellular location	Cytoplasm HAMAP MF_00118_B.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct translation elongation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
trxA	P52231	5	EBI-862703,EBI-862916

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 399	399	Elongation factor Tu HAMAP MF_00118_B		PRO_0000091418
Regions
	Nucleotide binding	19 – 26	8	GTP By similarity
	Nucleotide binding	81 – 85	5	GTP By similarity
	Nucleotide binding	136 – 139	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P74227 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 4F059A997D89A58C	FASTA	399	43,733
	10 20 30 40 50 60 MARAKFERTK DHVNIGTIGH VDHGKTTLTA AITMTLAELG GAKARKYEDI DAAPEEKARG 70 80 90 100 110 120 ITINTAHVEY ETDSRHYAHV DCPGHADYVK NMITGAAQMD GAILVVSAAD GPMPQTREHI 130 140 150 160 170 180 LLAKQVGVPK LVVFLNKKDM VDDEELLELV ELEVRELLSD YDFPGDDIPI VAGSALKAIE 190 200 210 220 230 240 GEKEYKDAIL ELMKAVDDYI DTPEREVDKP FLMAVEDVFS ITGRGTVATG RIERGKVKVG 250 260 270 280 290 300 EEISIVGIKD TRKATVTGVE MFQKTLEEGM AGDNVGLLLR GIQKEDIERG MVLAKPGSIT 310 320 330 340 350 360 PHTEFEGEVY VLKKEEGGRH TPFFANYRPQ FYVRTTDVTG TIKSYTADDG SAVEMVMPGD 370 380 390 RIKMTVELIN PIAIEQGMRF AIREGGRTIG AGVVSKILK

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA18321.1.
PIR	S75862.
RefSeq	NP_441641.1. NC_000911.1.
3D structure databases
ProteinModelPortal	P74227.
SMR	P74227. Positions 2-399.
ModBase	Search...
Protein-protein interaction databases
IntAct	P74227. 4 interactions.
STRING	P74227.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	952743.
GenomeReviews	Gene locus sll1099 in contig BA000022_GR.
KEGG	syn:sll1099.
NMPDR	fig|1148.1.peg.1742.
PATRIC	23840733. VBISynSp132158_1921.
Phylogenomic databases
eggNOG	COG0050.
HOGENOM	HBG307581.
OMA	MEKGLRF.
PhylomeDB	P74227.
ProtClustDB	PRK00049.
Enzyme and pathway databases
BioCyc	SSP1148:SLL1099-MONOMER.
Family and domain databases
HAMAP	MF_00118_B. EF_Tu_B. [Tree]
InterPro	IPR000795. ProtSyn_GTP-bd. IPR005225. Small_GTP-bd_dom. IPR009001. Transl_elong_EF1A/Init_IF2_C. IPR004161. Transl_elong_EFTu/EF1A_2. IPR004541. Transl_elong_EFTu/EF1A_bac/org. IPR004160. Transl_elong_EFTu/EF1A_C. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
KO	K02358.
PANTHER	PTHR23115:SF31. Transl_elong_EFTu/EF1A_bac/org. 1 hit.
Pfam	PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF03143. GTP_EFTU_D3. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SUPFAM	SSF50465. Elong_init_C. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00485. EF-Tu. 1 hit. TIGR00231. Small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFTU_SYNY3

Accession

Primary (citable) accession number: P74227

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program