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P74208 (PYRG_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
CTP synthase

EC=6.3.4.2
Alternative name(s):
CTP synthetase
UTP--ammonia ligase
Gene names
Name:pyrG
Ordered Locus Names:sll1443
OrganismSynechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]
Taxonomic identifier1111708 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis

Protein attributes

Sequence length552 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen By similarity. HAMAP-Rule MF_01227

Catalytic activity

ATP + UTP + L-glutamine = ADP + phosphate + CTP + L-glutamate. HAMAP-Rule MF_01227

Enzyme regulation

Allosterically activated by GTP, when glutamine is the substrate. Inhibited by CTP By similarity. HAMAP-Rule MF_01227

Pathway

Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2. HAMAP-Rule MF_01227

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_01227

Sequence similarities

Belongs to the CTP synthase family.

Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
   Biological processPyrimidine biosynthesis
   DomainGlutamine amidotransferase
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_process'de novo' CTP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

glutamine metabolic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

CTP synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 552552CTP synthase HAMAP-Rule MF_01227
PRO_0000138241

Regions

Domain292 – 534243Glutamine amidotransferase type-1
Region1 – 254254Aminator domain HAMAP-Rule MF_01227

Sites

Active site3811Nucleophile By similarity
Active site5071 By similarity
Active site5091 By similarity

Sequences

Sequence LengthMass (Da)Tools
P74208 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 7DA823A1B21C7074

FASTA55261,515
        10         20         30         40         50         60 
MSKFVFVTGG VVSSIGKGIV AASLGRLLKS RHYSVSILKL DPYINVDPGT MSPFQHGEVF 

        70         80         90        100        110        120 
VTEDGAETDL DLGHYERFTD TSMSRLNSVT TGSIYQAVIN KERRGAYMGG TVQVIPHITN 

       130        140        150        160        170        180 
EIKERILRVA QNTNPDVVIT EIGGTVGDIE SLPFLEAIRQ FRKEVGRHNV IYMHVTLIPW 

       190        200        210        220        230        240 
IPAAKEMKTK PTQHSVKELR SIGIQPDILV CRCDRPLHPG MKEKLSEFCD VPVESVITCQ 

       250        260        270        280        290        300 
DASSIYEVPL ILEKEGLAHQ TLELLRMENR SPDLSQWQSL VEKMQSPHHD ITVALVGKYV 

       310        320        330        340        350        360 
QLSDAYLSVV EALGHAAIAS DSKLHLRWIS AEEIEAQGAA TFLKDVDGVL VPGGFGIRGV 

       370        380        390        400        410        420 
DGKVQAIEYA RENQLPFLGL CLGMQCSVIE WARNVAKLPE ANSAEFETET PNPVINLLPE 

       430        440        450        460        470        480 
QQDVVDLGGT MRLGLYPCRI APDTLAFSLY QKEVVYERHR HRYEFNNSYR TQFTDTGFVV 

       490        500        510        520        530        540 
SGTSPDGRLV EIVEYPHHPF FIACQFHPEF HSRPNQAHPL FSGFINAVLK RRNAPAKIAV 

       550 
NCHTVTEDHE PS 

« Hide

References

[1]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. expand/collapse author list , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000022 Genomic DNA. Translation: BAA18299.1.
PIRS75840.
RefSeqNP_441619.1. NC_000911.1.
YP_005651677.1. NC_017277.1.
YP_007451501.1. NC_020286.1.

3D structure databases

ProteinModelPortalP74208.
SMRP74208. Positions 2-532.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP74208. 4 interactions.
STRING1148.sll1443.

Proteomic databases

PaxDbP74208.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA18299; BAA18299; BAA18299.
GeneID12254332.
14617164.
953058.
KEGGsyn:sll1443.
syy:SYNGTS_1724.
syz:MYO_117420.
PATRIC23840679. VBISynSp132158_1894.

Phylogenomic databases

eggNOGCOG0504.
HOGENOMHOG000077515.
KOK01937.
OMAYEFNNAY.
OrthoDBEOG6RC3NR.
PhylomeDBP74208.
ProtClustDBPRK05380.

Enzyme and pathway databases

UniPathwayUPA00159; UER00277.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
HAMAPMF_01227. PyrG.
InterProIPR004468. CTP_synthase.
IPR017456. CTP_synthase_N.
IPR017926. GATASE.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR11550. PTHR11550. 1 hit.
PfamPF06418. CTP_synth_N. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00337. PyrG. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePYRG_SYNY3
AccessionPrimary (citable) accession number: P74208
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: April 16, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Synechocystis PCC 6803

Synechocystis (strain PCC 6803): entries and gene names

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways