Reviewed, UniProtKB/Swiss-Prot P74132 (HEMN_SYNY3)

Names and origin

Protein names

Recommended name:
    Oxygen-independent coproporphyrinogen-III oxidase
      Short name=Coproporphyrinogenase
      Short name=Coprogen oxidase
    EC=1.3.99.22

Gene names

Name:	hemN
Ordered Locus Names:	sll1876

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX By similarity.
Catalytic activity	Coproporphyrinogen-III + 2 S-adenosyl-L-methionine = protoporphyrinogen-IX + 2 CO2 + 2 L-methionine + 2 5'-deoxyadenosine.
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (AdoMet route): step 1/1.
Subunit structure	Monomer.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the anaerobic coproporphyrinogen-III oxidase family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW porphyrin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW coproporphyrinogen dehydrogenase activity Inferred from electronic annotation. Source: EC coproporphyrinogen oxidase activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 466	466	Oxygen-independent coproporphyrinogen-III oxidase		PRO_0000109954
Regions
	Region	118 – 119	2	S-adenosyl-L-methionine 2 binding By similarity
Sites
	Metal binding	66	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	70	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	73	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Binding site	60	1	S-adenosyl-L-methionine 1 By similarity
	Binding site	72	1	S-adenosyl-L-methionine 2; via carbonyl oxygen By similarity
	Binding site	117	1	S-adenosyl-L-methionine 1; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	150	1	S-adenosyl-L-methionine 1 By similarity
	Binding site	177	1	S-adenosyl-L-methionine 2 By similarity
	Binding site	189	1	S-adenosyl-L-methionine 2 By similarity
	Binding site	214	1	S-adenosyl-L-methionine 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P74132-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: D49735A905A5C572	FASTA	466	53,161
	10 20 30 40 50 60 MTTTFPTVEF SAELLNKYNQ GIPRYTSYPP ATELNKEFDP SDFQTAINLG NYKKTPLSLY 70 80 90 100 110 120 CHIPFCAKAC YFCGCNTIIT QHKPAVDPYL KAVAKQIALV APLVDQQRPV QQLHWGGGTP 130 140 150 160 170 180 NYLTLEQAEF LFNTITDAFP LAENAEISIE INPCYVDKDY IFALRQLGFN RISFGIQDFN 190 200 210 220 230 240 SQVQQAVNRI QPEAMLFQVM DWIRQANFDS VNVDLIYGLP HQNLATFRET LRKTAQLNPD 250 260 270 280 290 300 RIAVFNFAYV PWLKPVQKKM PESALPPAEE KLKIMQATIA DLTEQGYVFI GMDHFAKPDD 310 320 330 340 350 360 ELAIAQRRGE LHRNFQGYTT QPESDLLGFG ITSISMLQDV YAQNHKTLKA FYNALDREVM 370 380 390 400 410 420 PIEKGFKLSQ DDLIRRTVIK ELMCQFKLSA QELESKYNLG FDCDFNDYFA KELSALDVLE 430 440 450 460 ADGLLRRLGD GLEVTPRGRI LIRNIAAVFD TYLQNKSKQQ MFSRAI

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000022 Genomic DNA. Translation: BAA18218.1.
PIR	S75657.
RefSeq	NP_441538.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	955014.
GenomeReviews	Gene locus sll1876 in contig BA000022_GR.
KEGG	syn:sll1876.
NMPDR	fig|1148.1.peg.1639.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P74132.
OMA	P74132. HVPFCES.
Enzyme and pathway databases
BioCyc	SSP1148:SLL1876-MON.
Family and domain databases
InterPro	IPR006638. Elp3/MiaB/NifB. IPR004558. HemN. IPR010723. HemN_C. IPR007197. Radical_SAM. [Graphical view]
Pfam	PF06969. HemN_C. 1 hit. PF04055. Radical_SAM. 1 hit. [Graphical view]
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00538. hemN. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HEMN_SYNY3

Accession

Primary (citable) accession number: P74132

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 1, 1997
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)