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Protein

Anthranilate synthase component I-like protein

Gene

trpE2

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Anthranilate synthase component I-like protein (trpE2), Anthranilate synthase component 1 (trpE)
  2. Anthranilate phosphoribosyltransferase (trpD)
  3. N-(5'-phosphoribosyl)anthranilate isomerase (trpF)
  4. Indole-3-glycerol phosphate synthase (trpC)
  5. Tryptophan synthase beta chain (trpB), Tryptophan synthase alpha chain (trpA)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei69TryptophanBy similarity1
Metal bindingi333MagnesiumBy similarity1
Binding sitei441ChorismateBy similarity1
Binding sitei457Chorismate; via amide nitrogenBy similarity1
Metal bindingi470MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.

Names & Taxonomyi

Protein namesi
Recommended name:
Anthranilate synthase component I-like protein (EC:4.1.3.27)
Gene namesi
Name:trpE2
Ordered Locus Names:slr1979
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesMerismopediaceaeSynechocystis
Proteomesi
  • UP000001425 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001541151 – 485Anthranilate synthase component I-like proteinAdd BLAST485

Proteomic databases

PRIDEiP74130.

Interactioni

Subunit structurei

Tetramer of two components I and two components II.By similarity

Structurei

3D structure databases

ProteinModelPortaliP74130.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni271 – 273Tryptophan bindingBy similarity3
Regioni306 – 307Chorismate bindingBy similarity2
Regioni455 – 457Chorismate bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000025142.
InParanoidiP74130.
KOiK01665.
OMAiIKETFPM.
PhylomeDBiP74130.

Family and domain databases

Gene3Di3.60.120.10. 1 hit.
InterProiIPR005801. ADC_synthase.
IPR019999. Anth_synth_I-like.
IPR006805. Anth_synth_I_N.
IPR015890. Chorismate_C.
IPR010118. Para-NH2Bz/anthranilate_synth.
[Graphical view]
PfamiPF04715. Anth_synt_I_N. 1 hit.
PF00425. Chorismate_bind. 1 hit.
[Graphical view]
PRINTSiPR00095. ANTSNTHASEI.
SUPFAMiSSF56322. SSF56322. 1 hit.
TIGRFAMsiTIGR01824. PabB-clade2. 1 hit.

Sequencei

Sequence statusi: Complete.

P74130-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASIAHCSGG LVGAGNFDFI LGGSDPVGRH GLMELQPWHW CCLPLEGRTG
60 70 80 90 100
SEIFSQLFGH QGIATLLESP YPASPDHPHL GRYSLCAGQP RKGRLWTPKP
110 120 130 140 150
EEIFSFLNQL CPCNHDVNLT KNIPEHLPFH GGWLGWLGYD TAWAIEKLPY
160 170 180 190 200
SKADDLPFPV AYWYEPENFV ILDHQEQLLW LATTDQEKIK FFQTQLADKI
210 220 230 240 250
NSVSSPQVPP LNLTYTTDQD QYETMVNQAK QYIKAGDIFQ ANLTLRFIAK
260 270 280 290 300
TEQKLNSWQV YQHLQTINPS PFASYWRSPW GDVVSCSPER LVKLEGNVAQ
310 320 330 340 350
TRPIAGTRAR GKNLAEDEQL LQELLVNTKE LAEHIMLVDL ERNDLGRVCT
360 370 380 390 400
WGTVEVDELL AIERYSHVSH LVSNVKGILQ PDKTGVDLVK ALFPGGTITG
410 420 430 440 450
CPKIRCLEII EELEPVRRSL FYGSCGYWDQ RGNLDLNILI RTLLFTSGQV
460 470 480
TGQVGAGIVA DSDPAKEWLE SLQKAKALLA ALEGL
Length:485
Mass (Da):54,271
Last modified:February 1, 1997 - v1
Checksum:i4F25ECCB3897BC7C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA18216.1.
PIRiS75655.

Genome annotation databases

EnsemblBacteriaiBAA18216; BAA18216; BAA18216.
KEGGisyn:slr1979.
PATRICi23840495. VBISynSp132158_1805.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA18216.1.
PIRiS75655.

3D structure databases

ProteinModelPortaliP74130.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP74130.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA18216; BAA18216; BAA18216.
KEGGisyn:slr1979.
PATRICi23840495. VBISynSp132158_1805.

Phylogenomic databases

HOGENOMiHOG000025142.
InParanoidiP74130.
KOiK01665.
OMAiIKETFPM.
PhylomeDBiP74130.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.

Family and domain databases

Gene3Di3.60.120.10. 1 hit.
InterProiIPR005801. ADC_synthase.
IPR019999. Anth_synth_I-like.
IPR006805. Anth_synth_I_N.
IPR015890. Chorismate_C.
IPR010118. Para-NH2Bz/anthranilate_synth.
[Graphical view]
PfamiPF04715. Anth_synt_I_N. 1 hit.
PF00425. Chorismate_bind. 1 hit.
[Graphical view]
PRINTSiPR00095. ANTSNTHASEI.
SUPFAMiSSF56322. SSF56322. 1 hit.
TIGRFAMsiTIGR01824. PabB-clade2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRE2_SYNY3
AccessioniPrimary (citable) accession number: P74130
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Component I catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II provides glutamine amidotransferase activity.

Caution

This is a divergent form of trpE. It is not obvious if it is active in Trp biosynthesis.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.