P74120 (DER_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTPase Der

Alternative name(s):
GTP-binding protein EngA

Gene names

Name:	der
Synonyms:	engA
Ordered Locus Names:	slr1974

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechocystis › Synechocystis sp. PCC 6803

Protein attributes

Sequence length	452 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	GTPase that plays an essential role in the late steps of ribosome biogenesis By similarity. HAMAP-Rule MF_00195
Subunit structure	Associates with the 50S ribosomal subunit By similarity.
Sequence similarities	Belongs to the Era/MnmE GTP-binding protein family. Der subfamily. Contains 2 G (guanine nucleotide-binding) domains. Contains 1 KH-like domain.

Ontologies

Keywords
Biological process	Ribosome biogenesis
Domain	Repeat
Ligand	GTP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ribosome biogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	GTP binding Inferred from electronic annotation. Source: HAMAP nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 452	452	GTPase Der HAMAP-Rule MF_00195		PRO_0000179063
Regions
	Domain	15 – 121	107	G 1
	Domain	188 – 296	109	G 2
	Domain	352 – 439	88	KH-like
	Nucleotide binding	10 – 17	8	GTP 1 Potential
	Nucleotide binding	57 – 61	5	GTP 1 Potential
	Nucleotide binding	120 – 123	4	GTP 1 Potential
	Nucleotide binding	183 – 190	8	GTP 2 Potential
	Nucleotide binding	230 – 234	5	GTP 2 Potential
	Nucleotide binding	295 – 298	4	GTP 2 Potential

Sequences

	Sequence		Length	Mass (Da)
	P74120 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 7FF771EAF7D6CC76	FASTA	452	50,826
	10 20 30 40 50 60 MSLPIVAIIG RPNVGKSTFV NRLAGNQQAI VHDQPGITRD RTYRPAFWRD RDFQVVDTGG 70 80 90 100 110 120 LVFNDDSEFL PEIREQANLA LAEAKAAIFV VDGQQGPTAS DEEIAQWLRQ QSVPVILAVN 130 140 150 160 170 180 KCESPDQGAI QAAEFWHLGL GEPYPMSAIH GSGTGDLLDA LLEYLPAPQE EPEEDEIKVA 190 200 210 220 230 240 IVGRPNVGKS SLLNALTGEQ RAIVSPISGT TRDAIDMVVE RNGQKYRLID TAGIRRKKNV 250 260 270 280 290 300 DYGAEFFGIN RAFKAIRRAD VVLFVLDVLD GVTEQDLKLA GRIIEDGRAV VLVINKWDAV 310 320 330 340 350 360 EKDSYTIYEH REQLMARLYF MDWAEMIFVS AQTGLRVQKI LDCVDIAAQE HRRRVTTAVI 370 380 390 400 410 420 NEVLEEAVSW HSPPTTRQGK QGKIYYGTQV STQPPAIALF VNDPNRFNDN YRRYIEKQFR 430 440 450 KQLGFFGSPI RLFWRGKKVR EMEGSRNRAT KV

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA18206.1.
PIR	S75645.
RefSeq	NP_441526.1. NC_000911.1. YP_005651584.1. NC_017277.1. YP_007451408.1. NC_020286.1.
3D structure databases
ProteinModelPortal	P74120.
ModBase	Search...
Protein-protein interaction databases
IntAct	P74120. 11 interactions.
STRING	1148.slr1974.
Proteomic databases
PaxDb	P74120.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAA18206; BAA18206; BAA18206.
GeneID	12254236. 14617068. 954938.
KEGG	syn:slr1974. syy:SYNGTS_1631.
PATRIC	23840475. VBISynSp132158_1795.
Phylogenomic databases
eggNOG	COG1160.
HOGENOM	HOG000242861.
KO	K03977.
OMA	YLENTYR.
ProtClustDB	PRK00093.
Family and domain databases
Gene3D	3.30.300.20. 1 hit.
HAMAP	MF_00195. GTPase_Der.
InterPro	IPR003593. AAA+_ATPase. IPR016484. GTP-bd_EngA. IPR006073. GTP_binding_domain. IPR015946. KH_dom-like_a/b. IPR005225. Small_GTP-bd_dom. [Graphical view]
PANTHER	PTHR11649:SF5. PTHR11649:SF5. 1 hit.
Pfam	PF01926. MMR_HSR1. 2 hits. [Graphical view]
PIRSF	PIRSF006485. GTP-binding_EngA. 1 hit.
PRINTS	PR00326. GTP1OBG.
SMART	SM00382. AAA. 2 hits. [Graphical view]
TIGRFAMs	TIGR03594. GTPase_EngA. 1 hit. TIGR00231. small_GTP. 2 hits.
ProtoNet	Search...

Entry information

Entry name

DER_SYNY3

Accession

Primary (citable) accession number: P74120

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program