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P74007 (SPOT_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

EC=3.1.7.2
Alternative name(s):
Penta-phosphate guanosine-3'-pyrophosphohydrolase
Short name=(ppGpp)ase
Gene names
Name:spoT
Ordered Locus Names:slr1325
OrganismSynechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]
Taxonomic identifier1111708 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis

Protein attributes

Sequence length760 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into GDP. It may also be capable of catalyzing the synthesis of ppGpp By similarity.

Catalytic activity

Guanosine 3',5'-bis(diphosphate) + H2O = guanosine 5'-diphosphate + diphosphate.

Cofactor

Manganese By similarity.

Pathway

Purine metabolism; ppGpp biosynthesis; ppGpp from GDP: step 1/1.

Sequence similarities

Belongs to the RelA/SpoT family.

Contains 1 ACT domain.

Contains 1 HD domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 760760Probable guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase
PRO_0000166576

Regions

Domain76 – 175100HD
Domain685 – 75975ACT

Sequences

Sequence LengthMass (Da)Tools
P74007 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: CA276EA2286358F7

FASTA76086,569
        10         20         30         40         50         60 
MNAVAALPTP TIHTTCAQDI HDIELPQWLE DCLQQWQREI EQGQDETTAP HCLICRAFCF 

        70         80         90        100        110        120 
AYDLHAQQRR KSGEPYIAHP VAVAGLLRDL GGDEAMIAAG FLHDVVEDTD ISIEQIEALF 

       130        140        150        160        170        180 
GEETASLVEG VTKLSKFNFS STTEHQAENF RRMFLAMAKD IRVIVVKLAD RLHNMRTLDA 

       190        200        210        220        230        240 
LSPEKQRRIA RETKDIFAPL ANRLGIWRFK WELEDLSFKY LEPDSYRKIQ SLVVEKRGDR 

       250        260        270        280        290        300 
ESRLETVKDM LRFRLRDEGI EHFELQGRPK HLYGIYYKMT SQDKAFEEIY DIAALRIIVE 

       310        320        330        340        350        360 
SKGECYRALS VVHDVFKPIP GRFKDYIGLP KPNRYQSLHT TVLGLTSRPL EIQIRTEEMH 

       370        380        390        400        410        420 
HVAEYGIAAH WKYKESGGSE NATLTSTDEK FTWLRQLLDW QSDLKDAQEY VENLKQNLFD 

       430        440        450        460        470        480 
DDVYVFTPKG EVISLARGAT PVDFAYRIHT EVGHHMKGAR VNGQWLGVDT RLKNGDIVEI 

       490        500        510        520        530        540 
VTQKNSHPSL DWLNFVVTPS ARHRIRQWFK RSRRDENILR GRELLEKELG KTGLEALLKS 

       550        560        570        580        590        600 
EPMQKTAERC NYQNVEDLLA GLGYGEITSN SVVNRLRENN VNNVKNSQSS QEVTLASSPQ 

       610        620        630        640        650        660 
VHPPTPPATG KDNSPIAGIE GLLYHIAGCC HPLPGEPIMG VVTRGARGIS IHRQGCHNLE 

       670        680        690        700        710        720 
QMDGDRLIPV RWNPNTNNHQ TYPVDIVIEA IDRVGVLKDI LSRLSDNHIN VRNADVKTHL 

       730        740        750        760 
GRPAIISLKI DIHDYQQLLG IMAKIKNMSD VMDLRRVISG 

« Hide

References

[1]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. expand/collapse author list , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000022 Genomic DNA. Translation: BAA18078.1.
PIRS75517.
RefSeqNP_441398.1. NC_000911.1.
YP_005651456.1. NC_017277.1.
YP_007451280.1. NC_020286.1.

3D structure databases

ProteinModelPortalP74007.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP74007. 3 interactions.
STRING1148.slr1325.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA18078; BAA18078; BAA18078.
GeneID12254106.
14616938.
954753.
KEGGsyn:slr1325.
syy:SYNGTS_1503.
syz:MYO_115160.
PATRIC23840191. VBISynSp132158_1655.

Phylogenomic databases

eggNOGCOG0317.
HOGENOMHOG000018301.
KOK00951.
K01139.
OMALDWLNFV.
OrthoDBEOG6SV551.
PhylomeDBP74007.
ProtClustDBCLSK893102.

Enzyme and pathway databases

UniPathwayUPA00908; UER00886.

Family and domain databases

Gene3D3.10.20.30. 1 hit.
InterProIPR026020. (p)ppGpp_Synthase.
IPR002912. ACT_dom.
IPR012675. Beta-grasp_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view]
PANTHERPTHR21262:SF1. PTHR21262:SF1. 1 hit.
PfamPF01842. ACT. 1 hit.
PF01966. HD. 1 hit.
PF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view]
SMARTSM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view]
SUPFAMSSF81271. SSF81271. 1 hit.
TIGRFAMsTIGR00691. spoT_relA. 1 hit.
PROSITEPS51671. ACT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSPOT_SYNY3
AccessionPrimary (citable) accession number: P74007
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 1, 1997
Last modified: April 16, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Synechocystis PCC 6803

Synechocystis (strain PCC 6803): entries and gene names

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways