Reviewed, UniProtKB/Swiss-Prot P73925 (GLBN_SYNY3)

Names and origin

Protein names

Recommended name:
    Cyanoglobin
Alternative name(s):
    Hemoglobin
      Short name=Hb

Gene names

Name:	glbN
Ordered Locus Names:	slr2097

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	124 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Forms a very stable complex with oxygen. The oxygen dissociation rate is 0.011 s(-1).
Subunit structure	Monomer.
Sequence similarities	Belongs to the protozoan/cyanobacterial globin family.

Ontologies

Keywords
Biological process	Oxygen transport Transport
Ligand	Heme Iron Metal-binding
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	oxygen transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	heme binding Inferred from electronic annotation. Source: InterPro oxygen binding Inferred from electronic annotation. Source: InterPro oxygen transporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 124

124

Cyanoglobin

PRO_0000162646

Sites

Metal binding

46

1

Iron (heme distal ligand)

Metal binding

70

1

Iron (heme proximal ligand) Potential

Binding site

117

1

Heme (covalent; via tele nitrogen)

Experimental info

Mutagenesis

46

1

H → A: Changes iron coordination. Ref.2

Secondary structure

1

.

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124

Helix Strand Turn

Details...

Helix

4 – 8

5

Helix

11 – 26

16

Turn

29 – 31

3

Helix

32 – 35

4

Helix

40 – 55

16

Helix

58 – 60

3

Helix

63 – 69

7

Helix

71 – 76

6

Helix

81 – 96

16

Turn

97 – 99

3

Helix

102 – 112

11

Helix

115 – 121

7

Sequences

	Sequence		Length	Mass (Da)
	P73925-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 8F53B809B8358844	FASTA	124	13,868
	10 20 30 40 50 60 MSTLYEKLGG TTAVDLAVDK FYERVLQDDR IKHFFADVDM AKQRAHQKAF LTYAFGGTDK 70 80 90 100 110 120 YDGRYMREAH KELVENHGLN GEHFDAVAED LLATLKEMGV PEDLIAEVAA VAGAPAHKRD VLNQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Structural investigations of the hemoglobin of the cyanobacterium Synechocystis PCC6803 reveal a unique distal heme pocket." Couture M., Das T.K., Savard P.Y., Ouellet Y., Wittenberg J.B., Wittenberg B.A., Rousseau D.L., Guertin M. Eur. J. Biochem. 267:4770-4780(2000) [PubMed: 10903511] [Abstract] Cited for: CHARACTERIZATION, MUTAGENESIS OF HIS-46.
[3]	"Cloning, expression, purification, and preliminary characterization of a putative hemoglobin from the cyanobacterium Synechocystis sp. PCC 6803." Scott N.L., Lecomte J.T.J. Protein Sci. 9:587-597(2000) [PubMed: 10752621] [Abstract] Cited for: CHARACTERIZATION.
[4]	"Novel histidine-heme covalent linkage in a hemoglobin." Vu B.C., Jones A.D., Lecomte J.T.J. J. Am. Chem. Soc. 124:8544-8545(2002) [PubMed: 12121092] [Abstract] Cited for: COVALENT HEME ATTACHMENT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

BA000022 Genomic DNA. Translation: BAA17991.1.

PIR

S75129.

RefSeq

NP_441311.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1MWB	NMR	-	A	2-124	[»]
1RTX	X-ray	1.80	A	2-124	[»]
1S69	X-ray	1.68	A	1-124	[»]
1S6A	X-ray	1.69	A	1-124	[»]
2HZ1	X-ray	1.80	A	2-124	[»]
2HZ2	X-ray	2.00	A	2-124	[»]
2HZ3	X-ray	1.90	A	2-124	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

P73925.

Genome annotation databases

GeneID

954656.

GenomeReviews

Gene locus slr2097 in contig BA000022_GR.

KEGG

syn:slr2097.

NMPDR

fig|1148.1.peg.1412.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

P73925.

OMA

VDIFYRK.

Enzyme and pathway databases

BioCyc

SSP1148:SLR2097-MON.

Family and domain databases

InterPro

IPR012292. Globin.
IPR019795. Globin_bac-like_CS.
IPR001486. Globin_trunc_bac-like.
IPR016339. Globin_trunc_bac-like_HbN/cyan.
[Graphical view]

Gene3D

G3DSA:1.10.490.10. Globin_related. 1 hit.

Pfam

PF01152. Bac_globin. 1 hit.
[Graphical view]

PIRSF

PIRSF002030. Globin_Protozoa/Cyanobacteria. 1 hit.

ProDom

PD004840. Bac_globin. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS01213. GLOBIN_FAM_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

GLBN_SYNY3

Accession

Primary (citable) accession number: P73925

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	February 1, 1997
Last modified:	November 3, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)