Reviewed, UniProtKB/Swiss-Prot P73881 (COABC_SYNY3)

Names and origin

Protein names

Recommended name:
    Coenzyme A biosynthesis bifunctional protein coaBC
Alternative name(s):
    DNA/pantothenate metabolism flavoprotein
Including the following 2 domains:
    1- Recommended name:
            Phosphopantothenoylcysteine decarboxylase
                Short name=PPCDC
              EC=4.1.1.36
        Alternative name(s):
            CoaC
    2- Recommended name:
            Phosphopantothenate--cysteine ligase
              EC=6.3.2.5
        Alternative name(s):
            Phosphopantothenoylcysteine synthase
            PPC synthetase
              Short name=PPCS
            CoaB

Gene names

Name:	coaBC
Synonyms:	dfp
Ordered Locus Names:	sll0250

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	402 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes two steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine By similarity.
Catalytic activity	N-((R)-4'-phosphopantothenoyl)-L-cysteine = pantotheine 4'-phosphate + CO2. CTP + (R)-4'-phosphopantothenate + L-cysteine = CMP + PPi + N-((R)-4'-phosphopantothenoyl)-L-cysteine.
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Cofactor biosynthesis; coenzyme A biosynthesis; coenzyme A from pantothenate: step 2/5. Cofactor biosynthesis; coenzyme A biosynthesis; coenzyme A from pantothenate: step 3/5.
Subunit structure	Homododecamer, the coaB domains form homodimers.
Sequence similarities	In the N-terminal section; belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily. In the C-terminal section; belongs to the PPC synthetase family.

Ontologies

Keywords
Ligand	FMN Flavoprotein
Molecular function	Decarboxylase Ligase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	coenzyme A biosynthetic process Inferred from electronic annotation. Source: InterPro pantothenate catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro phosphopantothenate--cysteine ligase activity Inferred from electronic annotation. Source: EC phosphopantothenoylcysteine decarboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 402	402	Coenzyme A biosynthesis bifunctional protein coaBC		PRO_0000232697
Regions
	Region	1 – 191	191	Phosphopantothenoylcysteine decarboxylase
	Region	192 – 402	211	Phosphopantothenate--cysteine ligase
Sites
	Active site	159	1	Proton donor By similarity
	Binding site	125	1	Substrate By similarity
	Binding site	283	1	CTP By similarity
	Binding site	293	1	CTP By similarity
	Binding site	342	1	CTP By similarity
	Binding site	346	1	CTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P73881-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 7E3F73DE185EC97C	FASTA	402	43,419
	10 20 30 40 50 60 MVKGKRILIG VGGGIAAYKI CEVVSQLFQQ GAEVRVILTA EAEKFVTPLT FTTLARHPAY 70 80 90 100 110 120 GDADFWQPIH HRPLHIDLGE WADIFLIAPL TAHTLAKLGH GFADDLLSNT VLASSCPILL 130 140 150 160 170 180 APAMNTDMWE QEAVQRNLQQ LLGDRRYHLL APNGGLLACD RRGVGRLAEP AQIMHRLQAL 190 200 210 220 230 240 LFTGGQEDLR GKRILITAGG TQEYLDAVRF IGNPSTGKMG LALAQSAGDR GARVTLIHGP 250 260 270 280 290 300 IGDLPIPMGV ISMAVVNAEQ MEQALAQQAP LADWIVMAAA VADVKPAQTW TGKIAKQDLP 310 320 330 340 350 360 DSLPLAPVPD LIAQVSKQKR PHQLLVGFAA QAGDIVTPAK EKLQRKNLDV IVANPIDQPQ 370 380 390 400 SGFGTDSNQA VIIDRHGSQT AIAPCSKLAM AHRIWQYLLE RI

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000022 Genomic DNA. Translation: BAA17944.1.
PIR	S75082.
RefSeq	NP_441264.1.
3D structure databases
HSSP	HSSP built from PDB template 1MVL based on UniProtKB Q9SWE5.
ModBase	Search...
Genome annotation databases
GeneID	954606.
GenomeReviews	Gene locus sll0250 in contig BA000022_GR.
KEGG	syn:sll0250.
NMPDR	fig|1148.1.peg.1365.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P73881.
OMA	P73881. VTSGPTH.
Enzyme and pathway databases
BioCyc	SSP1148:SLL0250-MON.
Family and domain databases
InterPro	IPR005252. Bifunc_COABC. IPR007085. DNA/pantothenate-metab_flavo_C. IPR003382. Flavoprotein. [Graphical view]
Gene3D	G3DSA:3.40.50.10300. DNA/pantothenate-metab_flavo_C. 1 hit.
Pfam	PF04127. DFP. 1 hit. PF02441. Flavoprotein. 1 hit. [Graphical view]
TIGRFAMs	TIGR00521. coaBC_dfp. 1 hit.
ProtoNet	Search...

Entry information

Entry name

COABC_SYNY3

Accession

Primary (citable) accession number: P73881

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)