P73851 (SYD_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--tRNA ligase
EC=6.1.1.12

Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	slr1720

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechocystis › Synechocystis sp. PCC 6803

Protein attributes

Sequence length	599 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP-Rule MF_00044_B
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00044_B
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00044_B.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 599	599	Aspartate--tRNA ligase HAMAP-Rule MF_00044_B		PRO_0000110967

Sequences

	Sequence		Length	Mass (Da)
	P73851 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 2B89C952C82A5246	FASTA	599	67,209
	10 20 30 40 50 60 MRTHYCGDLR TTHLGETVTL YGWVDRRRDH GGVIFLDLRD RQGIVQIASS PDQTPASYPV 70 80 90 100 110 120 AEGLRNEYVV QVTGVVSKRP PESLNEKIPT GEIEIYADSI ILLNGVNQQL PFVVSSHEAE 130 140 150 160 170 180 QVREDVRLKY RYLDLRRARL SQNLQLRHQV VKAMRRFLED QENFLEIETP ILTRSTPEGA 190 200 210 220 230 240 RDYLVPSRVN PGEWYALPQS PQLFKQLLMV AGMDRYYQIA RCFRDEDLRA DRQPEFTQLD 250 260 270 280 290 300 MEMSFMGQEE ILDLNEALIC HIFKVVKNID LPRPFPRLTY QESMAKYGND RPDTRFGLEL 310 320 330 340 350 360 VDVSDLLGNT GFKVFSAAVS SGGSIKAIRV PGGNETISNV RIKPGGDLFK EATEAGAKGI 370 380 390 400 410 420 AYIRVRDNGE IDTIGAIKEN LDEAQVKTLL QLTQAEAGDL LLFGAGDTAT VDKSLSRLRL 430 440 450 460 470 480 VLGEQLGLID PDAINLLWIT DFPMFEWNSD EKRFEALHHP FTAPHPDDLG DLKTARAQAY 490 500 510 520 530 540 DLVMNGVEIG GGSLRIYQRE VQEKVFATIG LSPEEAHEKF GFLLDAFEYG TPPHGGIAYG 550 560 570 580 590 LDRLVMLLAK EDSIRDVIAF PKTQQASCLL TEAPAAVDKK QLKELSVAST YVPKVKVDD

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA17910.1.
PIR	S75048.
RefSeq	NP_441230.1. NC_000911.1. YP_005651287.1. NC_017277.1. YP_007451112.1. NC_020286.1.
3D structure databases
ProteinModelPortal	P73851.
ModBase	Search...
Protein-protein interaction databases
IntAct	P73851. 2 interactions.
STRING	1148.slr1720.
Proteomic databases
PaxDb	P73851.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAA17910; BAA17910; BAA17910.
GeneID	12255695. 14616768. 954572.
KEGG	syn:slr1720. syy:SYNGTS_1334.
PATRIC	23839800. VBISynSp132158_1461.
Phylogenomic databases
eggNOG	COG0173.
HOGENOM	HOG000275159.
KO	K01876.
OMA	QEWAKQR.
ProtClustDB	PRK00476.
Family and domain databases
Gene3D	2.40.50.140. 1 hit. 3.30.1360.30. 1 hit.
HAMAP	MF_00044_B. Asp_tRNA_synth_B.
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004524. Asp-tRNA-ligase_IIb_bac/mt. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
PANTHER	PTHR22594. PTHR22594. 1 hit. PTHR22594:SF5. PTHR22594:SF5. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF55261. SSF55261. 1 hit.
TIGRFAMs	TIGR00459. aspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_SYNY3

Accession

Primary (citable) accession number: P73851

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program