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P73851

- SYDND_SYNY3

UniProt

P73851 - SYDND_SYNY3

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Protein
Aspartate--tRNA(Asp/Asn) ligase
Gene
aspS, slr1720
Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn) By similarity.UniRule annotation

Catalytic activityi

ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-aspartyl-tRNA(Asx).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei30 – 301Important for tRNA non-discrimination By similarity
Binding sitei178 – 1781Aspartate By similarity
Binding sitei224 – 2241Aspartate By similarity
Binding sitei233 – 2331ATP By similarity
Binding sitei458 – 4581Aspartate By similarity
Binding sitei488 – 4881ATP By similarity
Binding sitei495 – 4951Aspartate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi224 – 2263ATP By similarity
Nucleotide bindingi540 – 5434ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aspartate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. aspartate-tRNA(Asn) ligase activity Source: UniProtKB-EC
  4. nucleic acid binding Source: InterPro

GO - Biological processi

  1. aspartyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate--tRNA(Asp/Asn) ligase (EC:6.1.1.23)
Alternative name(s):
Aspartyl-tRNA synthetase
Short name:
AspRS
Non-discriminating aspartyl-tRNA synthetase
Short name:
ND-AspRS
Gene namesi
Name:aspS
Ordered Locus Names:slr1720
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 599599Aspartate--tRNA(Asp/Asn) ligaseUniRule annotation
PRO_0000110967Add
BLAST

Proteomic databases

PaxDbiP73851.

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

IntActiP73851. 2 interactions.
STRINGi1148.slr1720.

Structurei

3D structure databases

ProteinModelPortaliP73851.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni202 – 2054Aspartate By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0173.
HOGENOMiHOG000275159.
KOiK01876.
OMAiIGYEIST.
OrthoDBiEOG68Q0NX.
PhylomeDBiP73851.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.1360.30. 1 hit.
HAMAPiMF_00044. Asp_tRNA_synth.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004524. Asp-tRNA-ligase_IIb_bac/mt.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR004115. GAD-like.
IPR029351. GAD_dom.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF5. PTHR22594:SF5. 1 hit.
PfamiPF02938. GAD. 1 hit.
PF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF55261. SSF55261. 1 hit.
TIGRFAMsiTIGR00459. aspS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P73851-1 [UniParc]FASTAAdd to Basket

« Hide

MRTHYCGDLR TTHLGETVTL YGWVDRRRDH GGVIFLDLRD RQGIVQIASS    50
PDQTPASYPV AEGLRNEYVV QVTGVVSKRP PESLNEKIPT GEIEIYADSI 100
ILLNGVNQQL PFVVSSHEAE QVREDVRLKY RYLDLRRARL SQNLQLRHQV 150
VKAMRRFLED QENFLEIETP ILTRSTPEGA RDYLVPSRVN PGEWYALPQS 200
PQLFKQLLMV AGMDRYYQIA RCFRDEDLRA DRQPEFTQLD MEMSFMGQEE 250
ILDLNEALIC HIFKVVKNID LPRPFPRLTY QESMAKYGND RPDTRFGLEL 300
VDVSDLLGNT GFKVFSAAVS SGGSIKAIRV PGGNETISNV RIKPGGDLFK 350
EATEAGAKGI AYIRVRDNGE IDTIGAIKEN LDEAQVKTLL QLTQAEAGDL 400
LLFGAGDTAT VDKSLSRLRL VLGEQLGLID PDAINLLWIT DFPMFEWNSD 450
EKRFEALHHP FTAPHPDDLG DLKTARAQAY DLVMNGVEIG GGSLRIYQRE 500
VQEKVFATIG LSPEEAHEKF GFLLDAFEYG TPPHGGIAYG LDRLVMLLAK 550
EDSIRDVIAF PKTQQASCLL TEAPAAVDKK QLKELSVAST YVPKVKVDD 599
Length:599
Mass (Da):67,209
Last modified:February 1, 1997 - v1
Checksum:i2B89C952C82A5246
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000022 Genomic DNA. Translation: BAA17910.1.
PIRiS75048.
RefSeqiNP_441230.1. NC_000911.1.
YP_005651287.1. NC_017277.1.
YP_007451112.1. NC_020286.1.

Genome annotation databases

EnsemblBacteriaiBAA17910; BAA17910; BAA17910.
GeneIDi954572.
KEGGisyn:slr1720.
syy:SYNGTS_1334.
syz:MYO_113460.
PATRICi23839800. VBISynSp132158_1461.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000022 Genomic DNA. Translation: BAA17910.1 .
PIRi S75048.
RefSeqi NP_441230.1. NC_000911.1.
YP_005651287.1. NC_017277.1.
YP_007451112.1. NC_020286.1.

3D structure databases

ProteinModelPortali P73851.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P73851. 2 interactions.
STRINGi 1148.slr1720.

Proteomic databases

PaxDbi P73851.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA17910 ; BAA17910 ; BAA17910 .
GeneIDi 954572.
KEGGi syn:slr1720.
syy:SYNGTS_1334.
syz:MYO_113460.
PATRICi 23839800. VBISynSp132158_1461.

Phylogenomic databases

eggNOGi COG0173.
HOGENOMi HOG000275159.
KOi K01876.
OMAi IGYEIST.
OrthoDBi EOG68Q0NX.
PhylomeDBi P73851.

Family and domain databases

Gene3Di 2.40.50.140. 1 hit.
3.30.1360.30. 1 hit.
HAMAPi MF_00044. Asp_tRNA_synth.
InterProi IPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004524. Asp-tRNA-ligase_IIb_bac/mt.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR004115. GAD-like.
IPR029351. GAD_dom.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view ]
PANTHERi PTHR22594. PTHR22594. 1 hit.
PTHR22594:SF5. PTHR22594:SF5. 1 hit.
Pfami PF02938. GAD. 1 hit.
PF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view ]
PRINTSi PR01042. TRNASYNTHASP.
SUPFAMi SSF50249. SSF50249. 1 hit.
SSF55261. SSF55261. 1 hit.
TIGRFAMsi TIGR00459. aspS_bact. 1 hit.
PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.

Entry informationi

Entry nameiSYDND_SYNY3
AccessioniPrimary (citable) accession number: P73851
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: July 9, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3

Similar proteinsi