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P73851

- SYDND_SYNY3

UniProt

P73851 - SYDND_SYNY3

Protein

Aspartate--tRNA(Asp/Asn) ligase

Gene

aspS

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).UniRule annotation

    Catalytic activityi

    ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-aspartyl-tRNA(Asx).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei30 – 301Important for tRNA non-discriminationUniRule annotation
    Binding sitei178 – 1781AspartateUniRule annotation
    Binding sitei224 – 2241AspartateUniRule annotation
    Binding sitei233 – 2331ATPUniRule annotation
    Binding sitei458 – 4581AspartateUniRule annotation
    Binding sitei488 – 4881ATPUniRule annotation
    Binding sitei495 – 4951AspartateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi224 – 2263ATPUniRule annotation
    Nucleotide bindingi540 – 5434ATPUniRule annotation

    GO - Molecular functioni

    1. aspartate-tRNA(Asn) ligase activity Source: UniProtKB-EC
    2. aspartate-tRNA ligase activity Source: UniProtKB-HAMAP
    3. ATP binding Source: UniProtKB-HAMAP
    4. nucleic acid binding Source: InterPro

    GO - Biological processi

    1. aspartyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aspartate--tRNA(Asp/Asn) ligaseUniRule annotation (EC:6.1.1.23UniRule annotation)
    Alternative name(s):
    Aspartyl-tRNA synthetaseUniRule annotation
    Short name:
    AspRSUniRule annotation
    Non-discriminating aspartyl-tRNA synthetaseUniRule annotation
    Short name:
    ND-AspRSUniRule annotation
    Gene namesi
    Name:aspSUniRule annotation
    Ordered Locus Names:slr1720
    OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
    Taxonomic identifieri1111708 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
    ProteomesiUP000001425: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 599599Aspartate--tRNA(Asp/Asn) ligasePRO_0000110967Add
    BLAST

    Proteomic databases

    PaxDbiP73851.

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    IntActiP73851. 2 interactions.
    STRINGi1148.slr1720.

    Structurei

    3D structure databases

    ProteinModelPortaliP73851.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni202 – 2054AspartateUniRule annotation

    Sequence similaritiesi

    Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0173.
    HOGENOMiHOG000275159.
    KOiK01876.
    OMAiIGYEIST.
    OrthoDBiEOG68Q0NX.
    PhylomeDBiP73851.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    3.30.1360.30. 1 hit.
    HAMAPiMF_00044. Asp_tRNA_synth.
    InterProiIPR004364. aa-tRNA-synt_II.
    IPR018150. aa-tRNA-synt_II-like.
    IPR006195. aa-tRNA-synth_II.
    IPR004524. Asp-tRNA-ligase_IIb_bac/mt.
    IPR002312. Asp/Asn-tRNA-synth_IIb.
    IPR004115. GAD-like.
    IPR029351. GAD_dom.
    IPR012340. NA-bd_OB-fold.
    IPR004365. NA-bd_OB_tRNA.
    [Graphical view]
    PANTHERiPTHR22594. PTHR22594. 1 hit.
    PTHR22594:SF5. PTHR22594:SF5. 1 hit.
    PfamiPF02938. GAD. 1 hit.
    PF00152. tRNA-synt_2. 1 hit.
    PF01336. tRNA_anti-codon. 1 hit.
    [Graphical view]
    PRINTSiPR01042. TRNASYNTHASP.
    SUPFAMiSSF50249. SSF50249. 1 hit.
    SSF55261. SSF55261. 1 hit.
    TIGRFAMsiTIGR00459. aspS_bact. 1 hit.
    PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P73851-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRTHYCGDLR TTHLGETVTL YGWVDRRRDH GGVIFLDLRD RQGIVQIASS    50
    PDQTPASYPV AEGLRNEYVV QVTGVVSKRP PESLNEKIPT GEIEIYADSI 100
    ILLNGVNQQL PFVVSSHEAE QVREDVRLKY RYLDLRRARL SQNLQLRHQV 150
    VKAMRRFLED QENFLEIETP ILTRSTPEGA RDYLVPSRVN PGEWYALPQS 200
    PQLFKQLLMV AGMDRYYQIA RCFRDEDLRA DRQPEFTQLD MEMSFMGQEE 250
    ILDLNEALIC HIFKVVKNID LPRPFPRLTY QESMAKYGND RPDTRFGLEL 300
    VDVSDLLGNT GFKVFSAAVS SGGSIKAIRV PGGNETISNV RIKPGGDLFK 350
    EATEAGAKGI AYIRVRDNGE IDTIGAIKEN LDEAQVKTLL QLTQAEAGDL 400
    LLFGAGDTAT VDKSLSRLRL VLGEQLGLID PDAINLLWIT DFPMFEWNSD 450
    EKRFEALHHP FTAPHPDDLG DLKTARAQAY DLVMNGVEIG GGSLRIYQRE 500
    VQEKVFATIG LSPEEAHEKF GFLLDAFEYG TPPHGGIAYG LDRLVMLLAK 550
    EDSIRDVIAF PKTQQASCLL TEAPAAVDKK QLKELSVAST YVPKVKVDD 599
    Length:599
    Mass (Da):67,209
    Last modified:February 1, 1997 - v1
    Checksum:i2B89C952C82A5246
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000022 Genomic DNA. Translation: BAA17910.1.
    PIRiS75048.
    RefSeqiNP_441230.1. NC_000911.1.
    YP_005651287.1. NC_017277.1.
    YP_007451112.1. NC_020286.1.

    Genome annotation databases

    EnsemblBacteriaiBAA17910; BAA17910; BAA17910.
    GeneIDi954572.
    KEGGisyn:slr1720.
    syy:SYNGTS_1334.
    syz:MYO_113460.
    PATRICi23839800. VBISynSp132158_1461.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000022 Genomic DNA. Translation: BAA17910.1 .
    PIRi S75048.
    RefSeqi NP_441230.1. NC_000911.1.
    YP_005651287.1. NC_017277.1.
    YP_007451112.1. NC_020286.1.

    3D structure databases

    ProteinModelPortali P73851.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P73851. 2 interactions.
    STRINGi 1148.slr1720.

    Proteomic databases

    PaxDbi P73851.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA17910 ; BAA17910 ; BAA17910 .
    GeneIDi 954572.
    KEGGi syn:slr1720.
    syy:SYNGTS_1334.
    syz:MYO_113460.
    PATRICi 23839800. VBISynSp132158_1461.

    Phylogenomic databases

    eggNOGi COG0173.
    HOGENOMi HOG000275159.
    KOi K01876.
    OMAi IGYEIST.
    OrthoDBi EOG68Q0NX.
    PhylomeDBi P73851.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    3.30.1360.30. 1 hit.
    HAMAPi MF_00044. Asp_tRNA_synth.
    InterProi IPR004364. aa-tRNA-synt_II.
    IPR018150. aa-tRNA-synt_II-like.
    IPR006195. aa-tRNA-synth_II.
    IPR004524. Asp-tRNA-ligase_IIb_bac/mt.
    IPR002312. Asp/Asn-tRNA-synth_IIb.
    IPR004115. GAD-like.
    IPR029351. GAD_dom.
    IPR012340. NA-bd_OB-fold.
    IPR004365. NA-bd_OB_tRNA.
    [Graphical view ]
    PANTHERi PTHR22594. PTHR22594. 1 hit.
    PTHR22594:SF5. PTHR22594:SF5. 1 hit.
    Pfami PF02938. GAD. 1 hit.
    PF00152. tRNA-synt_2. 1 hit.
    PF01336. tRNA_anti-codon. 1 hit.
    [Graphical view ]
    PRINTSi PR01042. TRNASYNTHASP.
    SUPFAMi SSF50249. SSF50249. 1 hit.
    SSF55261. SSF55261. 1 hit.
    TIGRFAMsi TIGR00459. aspS_bact. 1 hit.
    PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
      Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
      , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PCC 6803 / Kazusa.

    Entry informationi

    Entry nameiSYDND_SYNY3
    AccessioniPrimary (citable) accession number: P73851
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Synechocystis PCC 6803
      Synechocystis (strain PCC 6803): entries and gene names

    External Data

    Dasty 3