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Protein

Aspartate--tRNA(Asp/Asn) ligase

Gene

aspS

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).UniRule annotation

Catalytic activityi

ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-aspartyl-tRNA(Asx).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei30 – 301Important for tRNA non-discriminationUniRule annotation
Binding sitei178 – 1781AspartateUniRule annotation
Binding sitei224 – 2241AspartateUniRule annotation
Binding sitei233 – 2331ATPUniRule annotation
Binding sitei458 – 4581AspartateUniRule annotation
Binding sitei488 – 4881ATPUniRule annotation
Binding sitei495 – 4951AspartateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi224 – 2263ATPUniRule annotation
Nucleotide bindingi540 – 5434ATPUniRule annotation

GO - Molecular functioni

  1. aspartate-tRNA(Asn) ligase activity Source: UniProtKB-EC
  2. aspartate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. ATP binding Source: UniProtKB-HAMAP
  4. nucleic acid binding Source: InterPro

GO - Biological processi

  1. aspartyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate--tRNA(Asp/Asn) ligaseUniRule annotation (EC:6.1.1.23UniRule annotation)
Alternative name(s):
Aspartyl-tRNA synthetaseUniRule annotation
Short name:
AspRSUniRule annotation
Non-discriminating aspartyl-tRNA synthetaseUniRule annotation
Short name:
ND-AspRSUniRule annotation
Gene namesi
Name:aspSUniRule annotation
Ordered Locus Names:slr1720
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 599599Aspartate--tRNA(Asp/Asn) ligasePRO_0000110967Add
BLAST

Proteomic databases

PaxDbiP73851.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

IntActiP73851. 2 interactions.
STRINGi1148.slr1720.

Structurei

3D structure databases

ProteinModelPortaliP73851.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni202 – 2054AspartateUniRule annotation

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0173.
HOGENOMiHOG000275159.
InParanoidiP73851.
KOiK01876.
OMAiYQLDVEM.
OrthoDBiEOG68Q0NX.
PhylomeDBiP73851.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.1360.30. 1 hit.
HAMAPiMF_00044. Asp_tRNA_synth.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004524. Asp-tRNA-ligase_IIb_bac/mt.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR004115. GAD-like.
IPR029351. GAD_dom.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF5. PTHR22594:SF5. 1 hit.
PfamiPF02938. GAD. 1 hit.
PF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF55261. SSF55261. 1 hit.
TIGRFAMsiTIGR00459. aspS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P73851-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRTHYCGDLR TTHLGETVTL YGWVDRRRDH GGVIFLDLRD RQGIVQIASS
60 70 80 90 100
PDQTPASYPV AEGLRNEYVV QVTGVVSKRP PESLNEKIPT GEIEIYADSI
110 120 130 140 150
ILLNGVNQQL PFVVSSHEAE QVREDVRLKY RYLDLRRARL SQNLQLRHQV
160 170 180 190 200
VKAMRRFLED QENFLEIETP ILTRSTPEGA RDYLVPSRVN PGEWYALPQS
210 220 230 240 250
PQLFKQLLMV AGMDRYYQIA RCFRDEDLRA DRQPEFTQLD MEMSFMGQEE
260 270 280 290 300
ILDLNEALIC HIFKVVKNID LPRPFPRLTY QESMAKYGND RPDTRFGLEL
310 320 330 340 350
VDVSDLLGNT GFKVFSAAVS SGGSIKAIRV PGGNETISNV RIKPGGDLFK
360 370 380 390 400
EATEAGAKGI AYIRVRDNGE IDTIGAIKEN LDEAQVKTLL QLTQAEAGDL
410 420 430 440 450
LLFGAGDTAT VDKSLSRLRL VLGEQLGLID PDAINLLWIT DFPMFEWNSD
460 470 480 490 500
EKRFEALHHP FTAPHPDDLG DLKTARAQAY DLVMNGVEIG GGSLRIYQRE
510 520 530 540 550
VQEKVFATIG LSPEEAHEKF GFLLDAFEYG TPPHGGIAYG LDRLVMLLAK
560 570 580 590
EDSIRDVIAF PKTQQASCLL TEAPAAVDKK QLKELSVAST YVPKVKVDD
Length:599
Mass (Da):67,209
Last modified:February 1, 1997 - v1
Checksum:i2B89C952C82A5246
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA17910.1.
PIRiS75048.
RefSeqiNP_441230.1. NC_000911.1.
YP_005651287.1. NC_017277.1.
YP_007451112.1. NC_020286.1.

Genome annotation databases

EnsemblBacteriaiBAA17910; BAA17910; BAA17910.
GeneIDi954572.
KEGGisyn:slr1720.
PATRICi23839800. VBISynSp132158_1461.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA17910.1.
PIRiS75048.
RefSeqiNP_441230.1. NC_000911.1.
YP_005651287.1. NC_017277.1.
YP_007451112.1. NC_020286.1.

3D structure databases

ProteinModelPortaliP73851.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP73851. 2 interactions.
STRINGi1148.slr1720.

Proteomic databases

PaxDbiP73851.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA17910; BAA17910; BAA17910.
GeneIDi954572.
KEGGisyn:slr1720.
PATRICi23839800. VBISynSp132158_1461.

Phylogenomic databases

eggNOGiCOG0173.
HOGENOMiHOG000275159.
InParanoidiP73851.
KOiK01876.
OMAiYQLDVEM.
OrthoDBiEOG68Q0NX.
PhylomeDBiP73851.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.1360.30. 1 hit.
HAMAPiMF_00044. Asp_tRNA_synth.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004524. Asp-tRNA-ligase_IIb_bac/mt.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR004115. GAD-like.
IPR029351. GAD_dom.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF5. PTHR22594:SF5. 1 hit.
PfamiPF02938. GAD. 1 hit.
PF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF55261. SSF55261. 1 hit.
TIGRFAMsiTIGR00459. aspS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.

Entry informationi

Entry nameiSYDND_SYNY3
AccessioniPrimary (citable) accession number: P73851
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: January 7, 2015
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.