true1997-11-012024-03-27144PHAB_SYNY3Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions.Kaneko T.Sato S.Kotani H.Tanaka A.Asamizu E.Nakamura Y.Miyajima N.Hirosawa M.Sugiura M.Sasamoto S.Kimura T.Hosouchi T.Matsuno A.Muraki A.Nakazaki N.Naruo K.Okumura S.Shimpo S.Takeuchi C.Wada T.Watanabe A.Yamada M.Yasuda M.Tabata S.doi:10.1093/dnares/3.3.1091996DNA Res.3109-136NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]PCC 6803 / KazusaIdentification and analysis of the polyhydroxyalkanoate-specific beta-ketothiolase and acetoacetyl coenzyme A reductase genes in the cyanobacterium Synechocystis sp. strain PCC6803.Taroncher-Oldenburg G.Nishina K.Stephanopoulos G.doi:10.1128/aem.66.10.4440-4448.20002000Appl. Environ. Microbiol.664440-4448FUNCTIONDISRUPTION PHENOTYPEEXPRESSION IN E.COLIATCC 27184 / PCC 6803 / N-1Structure-directed construction of a high-performance version of the enzyme FabG from the photosynthetic microorganism Synechocystis sp. PCC 6803.Liu Y.Feng Y.Cao X.Li X.Xue S.doi:10.1016/j.febslet.2015.09.0012015FEBS Lett.5893052-3057X-RAY CRYSTALLOGRAPHY (2.89 ANGSTROMS)FUNCTIONPATHWAYACTIVE SITEMUTAGENESIS OF SER-134; TYR-147 AND LYS-1512.89A/B/C=1-240BacteriaBKR_SDR_cNAD(P)-binding Rossmann-like DomainNAD(P)-bd_dom_sfSc_DH/Rdtase_CSSDR_fam3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE FABGadh_short_C2GDHRDHSDRFAMILYPKS_KRNAD(P)-binding Rossmann-fold domainsADH_SHORTAcetoacetyl-CoA reductase1.1.1.36phaBslr1994Catalyzes the reduction of acetoacetyl-CoA to (R)-3-hydroxybutyryl-CoA (PubMed:26358291). When expressed in E.coli with Synechocystis PhaA, PhaC and PhaE confers the ability to synthesize up to 12% (w/w) poly(3-hydroxybutyrate) (PHB) depending on the carbon source (PubMed:11010896).Double deletion of phaA and phaB leads to loss of synthesis of PHB, no visible growth phenotype.Poly(3-hydroxyalkanoic acids) (PHA), of which PHB is among the most common compounds, are prokaryotic intracellular storage compounds with potential uses as a renewable, biodegradable thermoplastic. Cyanobacterial PHB synthesis is particularly attractive as cyanobacteria use CO(2) as the carbon source.Nitrogen-free medium induces chlorosis in Synechocystis, leading to the degradation of the photosynthetic apparatus and concomitant accumulation of cytoplasmic polyhydroxyalkanoic acid (PHA) granules which in this cyanobacterium are composed of PHB.Belongs to the short-chain dehydrogenases/reductases (SDR) family.Acetoacetyl-CoA reductase253331240Proton acceptor1471820828613414114417718017817912% enzymatic activity.ANo enzymatic activity.A5% enzymatic activity.A151581014192930323440515359737881919396106108124127132135139145165166168170185187190197208219221223231235substrate1997-02-01125333d7e430dcf4ec53d592fc6280cd71de9dMLSLGLEDKVIVVTGGNRGIGAAIVKLLQEMGAKVAFTDLATDGGNTEALGVVANVTDLESMTAAAAEITDKLGPVYGVVANAGITKDNFFPKLTPADWDAVLNVNLKGVAYSIKPFIEGMYERKAGSIVAISSISGERGNVGQTNYSATKAGVIGMMKSLAREGARYGVRANAVAPGFIDTEMTLAIREDIREKITKEIPFRRFGKPEEIAWAVAFLLSPVASSYVTGEVLRVNGAHHTtruetruetruetruetruetruetruetruetruetrue