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P73810 (TOP1_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 1

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene names
Name:topA
Ordered Locus Names:slr2058
OrganismSynechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]
Taxonomic identifier1111708 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis

Protein attributes

Sequence length898 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. HAMAP-Rule MF_00952

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. HAMAP-Rule MF_00952

Cofactor

Magnesium. Binds two Mg2+ per subunit By similarity. HAMAP-Rule MF_00952

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00952

Sequence similarities

Belongs to the type IA topoisomerase family.

Contains 1 Toprim domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 898898DNA topoisomerase 1 HAMAP-Rule MF_00952
PRO_0000145168

Regions

Domain2 – 126125Toprim
Region175 – 1806Interaction with DNA By similarity

Sites

Active site3201O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Metal binding81Magnesium 1; catalytic By similarity
Metal binding951Magnesium 1; catalytic By similarity
Metal binding951Magnesium 2 By similarity
Metal binding971Magnesium 2 By similarity
Site321Interaction with DNA By similarity
Site1511Interaction with DNA By similarity
Site1521Interaction with DNA By similarity
Site1551Interaction with DNA By similarity
Site1601Interaction with DNA By similarity
Site1671Interaction with DNA By similarity
Site3221Interaction with DNA By similarity
Site5151Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
P73810 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 9416665977398EB3

FASTA89899,340
        10         20         30         40         50         60 
MSKLVIVESP TKARTIRNYL PQDYRVEASM GHVRDLPASA EEVPAAYKDK SWANLGVNVE 

        70         80         90        100        110        120 
DHFSPLYVIP KSKKKVVKEL QTALKNADEV ILATDEDREG ESISWHLLQL LQPKVPIKRM 

       130        140        150        160        170        180 
VFHEITQEAI RSALENCRDI DENLVHAQET RRILDRLVGY TLSPLLWKKI AWGLSAGRVQ 

       190        200        210        220        230        240 
SVAVRLIVQR ERARRAFKTA GYWDLKAELE QNKNPFQAKL MTLGGTKLAN GSDFDPNTGA 

       250        260        270        280        290        300 
LLPDKQVVVL DEAQAIALKE RLTGKPWAVV NTEEKPGVRK PSPPFTTSTL QQEANRKLGI 

       310        320        330        340        350        360 
SARDTMRVAQ KLYEEGYITY MRTDSVHLSD QAVTAARNCV QQMYGKEYLS PQPKQYTTKS 

       370        380        390        400        410        420 
KGAQEAHEAI RPAGTEFRIP NQTGLKDREL ALYELIWKRT VACQMADARI TQLSVLLKVE 

       430        440        450        460        470        480 
DAEFRAAGKR IDFPGYFRAY VEGSDDPDAA LENQEVILPP LKVGDRPNCR EIDTVGHETQ 

       490        500        510        520        530        540 
PPARYTEASL VKTLESEGVG RPSTYASIIG TIIDRGYVQM RSKALTPTFT AFAVVSLLES 

       550        560        570        580        590        600 
HFPDLVDTGF TSRMEQKLDE IAIGKTQWLP YLQGFFLGES GLENQVKVRQ DQIDPAIAKA 

       610        620        630        640        650        660 
IELENLAAKV KIGKFGPYIE IPQGEEIITA SIPQDLTPAD LNPEQVAVLL KQKTEGPDKV 

       670        680        690        700        710        720 
GIHPETGEPI FILIGAYGPY VQLGEATEEN KKPKRTSLPK GVKPEDVTLE MAVGLLALPR 

       730        740        750        760        770        780 
NLGEHPESGK NIKASLGRFG PYVVHDQGKD GKDYRSLKGD DDVLTITLER ALELLAEPKR 

       790        800        810        820        830        840 
GRGSRTPLKQ LGLHPEDQEP VNLFKGPYGV YIKHGKVNAS LPEGETEETI TLEKALPLLA 

       850        860        870        880        890 
EKAGSGKKTS RKKATTTAKG TKKTTSKKAS ATGTAKKTTT KRTTRKKAAS PDQSSEAG 

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References

[1]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. expand/collapse author list , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000022 Genomic DNA. Translation: BAA17864.1.
PIRS74903.
RefSeqNP_441184.1. NC_000911.1.
YP_005651241.1. NC_017277.1.
YP_007451066.1. NC_020286.1.

3D structure databases

ProteinModelPortalP73810.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING1148.slr2058.

Proteomic databases

PaxDbP73810.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA17864; BAA17864; BAA17864.
GeneID954520.
KEGGsyn:slr2058.
syy:SYNGTS_1288.
syz:MYO_113000.
PATRIC23839689. VBISynSp132158_1406.

Phylogenomic databases

eggNOGCOG1754.
HOGENOMHOG000004020.
KOK03168.
OMAPSCNTGE.
OrthoDBEOG6S7XQ9.
PhylomeDBP73810.

Family and domain databases

Gene3D1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPMF_00952. Topoisom_1_prok.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR025589. Toprim_C_rpt.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF13368. Toprim_C_rpt. 3 hits.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. SSF56712. 1 hit.
TIGRFAMsTIGR01051. topA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP1_SYNY3
AccessionPrimary (citable) accession number: P73810
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: July 9, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Synechocystis PCC 6803

Synechocystis (strain PCC 6803): entries and gene names

SIMILARITY comments

Index of protein domains and families