P73810 (TOP1_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA topoisomerase 1
EC=5.99.1.2

Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme

Gene names

Name:	topA
Ordered Locus Names:	slr2058

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa)

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	898 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the type IA topoisomerase family. Contains 1 Toprim domain.

Ontologies

Keywords
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 898	898	DNA topoisomerase 1		PRO_0000145168
Regions
	Domain	2 – 123	122	Toprim
Sites
	Active site	320	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P73810 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 9416665977398EB3	FASTA	898	99,340
	10 20 30 40 50 60 MSKLVIVESP TKARTIRNYL PQDYRVEASM GHVRDLPASA EEVPAAYKDK SWANLGVNVE 70 80 90 100 110 120 DHFSPLYVIP KSKKKVVKEL QTALKNADEV ILATDEDREG ESISWHLLQL LQPKVPIKRM 130 140 150 160 170 180 VFHEITQEAI RSALENCRDI DENLVHAQET RRILDRLVGY TLSPLLWKKI AWGLSAGRVQ 190 200 210 220 230 240 SVAVRLIVQR ERARRAFKTA GYWDLKAELE QNKNPFQAKL MTLGGTKLAN GSDFDPNTGA 250 260 270 280 290 300 LLPDKQVVVL DEAQAIALKE RLTGKPWAVV NTEEKPGVRK PSPPFTTSTL QQEANRKLGI 310 320 330 340 350 360 SARDTMRVAQ KLYEEGYITY MRTDSVHLSD QAVTAARNCV QQMYGKEYLS PQPKQYTTKS 370 380 390 400 410 420 KGAQEAHEAI RPAGTEFRIP NQTGLKDREL ALYELIWKRT VACQMADARI TQLSVLLKVE 430 440 450 460 470 480 DAEFRAAGKR IDFPGYFRAY VEGSDDPDAA LENQEVILPP LKVGDRPNCR EIDTVGHETQ 490 500 510 520 530 540 PPARYTEASL VKTLESEGVG RPSTYASIIG TIIDRGYVQM RSKALTPTFT AFAVVSLLES 550 560 570 580 590 600 HFPDLVDTGF TSRMEQKLDE IAIGKTQWLP YLQGFFLGES GLENQVKVRQ DQIDPAIAKA 610 620 630 640 650 660 IELENLAAKV KIGKFGPYIE IPQGEEIITA SIPQDLTPAD LNPEQVAVLL KQKTEGPDKV 670 680 690 700 710 720 GIHPETGEPI FILIGAYGPY VQLGEATEEN KKPKRTSLPK GVKPEDVTLE MAVGLLALPR 730 740 750 760 770 780 NLGEHPESGK NIKASLGRFG PYVVHDQGKD GKDYRSLKGD DDVLTITLER ALELLAEPKR 790 800 810 820 830 840 GRGSRTPLKQ LGLHPEDQEP VNLFKGPYGV YIKHGKVNAS LPEGETEETI TLEKALPLLA 850 860 870 880 890 EKAGSGKKTS RKKATTTAKG TKKTTSKKAS ATGTAKKTTT KRTTRKKAAS PDQSSEAG

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA17864.1.
PIR	S74903.
RefSeq	NP_441184.1. NC_000911.1.
3D structure databases
ProteinModelPortal	P73810.
ModBase	Search...
Protein-protein interaction databases
STRING	P73810.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	954520.
GenomeReviews	Gene locus slr2058 in contig BA000022_GR.
KEGG	syn:slr2058.
NMPDR	fig|1148.1.peg.1285.
PATRIC	23839689. VBISynSp132158_1406.
Phylogenomic databases
eggNOG	COG0550.
HOGENOM	HBG512935.
OMA	KKVMPRL.
PhylomeDB	P73810.
ProtClustDB	PRK07561.
Enzyme and pathway databases
BioCyc	SSP1148:SLR2058-MONOMER.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOP1_SYNY3

Accession

Primary (citable) accession number: P73810

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program