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P73810

- TOP1_SYNY3

UniProt

P73810 - TOP1_SYNY3

Protein

DNA topoisomerase 1

Gene

topA

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

    Cofactori

    Magnesium. Binds two Mg2+ per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi8 – 81Magnesium 1; catalyticUniRule annotation
    Sitei32 – 321Interaction with DNAUniRule annotation
    Metal bindingi95 – 951Magnesium 1; catalyticUniRule annotation
    Metal bindingi95 – 951Magnesium 2UniRule annotation
    Metal bindingi97 – 971Magnesium 2UniRule annotation
    Sitei151 – 1511Interaction with DNAUniRule annotation
    Sitei152 – 1521Interaction with DNAUniRule annotation
    Sitei155 – 1551Interaction with DNAUniRule annotation
    Sitei160 – 1601Interaction with DNAUniRule annotation
    Sitei167 – 1671Interaction with DNAUniRule annotation
    Active sitei320 – 3201O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
    Sitei322 – 3221Interaction with DNAUniRule annotation
    Sitei515 – 5151Interaction with DNAUniRule annotation

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA topological change Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding, Magnesium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
    Alternative name(s):
    DNA topoisomerase IUniRule annotation
    Omega-protein
    Relaxing enzyme
    Swivelase
    Untwisting enzyme
    Gene namesi
    Name:topAUniRule annotation
    Ordered Locus Names:slr2058
    OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
    Taxonomic identifieri1111708 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
    ProteomesiUP000001425: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 898898DNA topoisomerase 1PRO_0000145168Add
    BLAST

    Proteomic databases

    PaxDbiP73810.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi1148.slr2058.

    Structurei

    3D structure databases

    ProteinModelPortaliP73810.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 126125ToprimUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni175 – 1806Interaction with DNAUniRule annotation

    Sequence similaritiesi

    Belongs to the type IA topoisomerase family.UniRule annotation
    Contains 1 Toprim domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1754.
    HOGENOMiHOG000004020.
    KOiK03168.
    OMAiPSCNTGE.
    OrthoDBiEOG6S7XQ9.
    PhylomeDBiP73810.

    Family and domain databases

    Gene3Di1.10.460.10. 2 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPiMF_00952. Topoisom_1_prok.
    InterProiIPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR005733. TopoI_bac-type.
    IPR028612. Topoisom_1_IA.
    IPR025589. Toprim_C_rpt.
    IPR006171. Toprim_domain.
    [Graphical view]
    PANTHERiPTHR11390. PTHR11390. 1 hit.
    PfamiPF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF13368. Toprim_C_rpt. 3 hits.
    [Graphical view]
    PRINTSiPR00417. PRTPISMRASEI.
    SMARTiSM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view]
    SUPFAMiSSF56712. SSF56712. 1 hit.
    TIGRFAMsiTIGR01051. topA_bact. 1 hit.
    PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P73810-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKLVIVESP TKARTIRNYL PQDYRVEASM GHVRDLPASA EEVPAAYKDK    50
    SWANLGVNVE DHFSPLYVIP KSKKKVVKEL QTALKNADEV ILATDEDREG 100
    ESISWHLLQL LQPKVPIKRM VFHEITQEAI RSALENCRDI DENLVHAQET 150
    RRILDRLVGY TLSPLLWKKI AWGLSAGRVQ SVAVRLIVQR ERARRAFKTA 200
    GYWDLKAELE QNKNPFQAKL MTLGGTKLAN GSDFDPNTGA LLPDKQVVVL 250
    DEAQAIALKE RLTGKPWAVV NTEEKPGVRK PSPPFTTSTL QQEANRKLGI 300
    SARDTMRVAQ KLYEEGYITY MRTDSVHLSD QAVTAARNCV QQMYGKEYLS 350
    PQPKQYTTKS KGAQEAHEAI RPAGTEFRIP NQTGLKDREL ALYELIWKRT 400
    VACQMADARI TQLSVLLKVE DAEFRAAGKR IDFPGYFRAY VEGSDDPDAA 450
    LENQEVILPP LKVGDRPNCR EIDTVGHETQ PPARYTEASL VKTLESEGVG 500
    RPSTYASIIG TIIDRGYVQM RSKALTPTFT AFAVVSLLES HFPDLVDTGF 550
    TSRMEQKLDE IAIGKTQWLP YLQGFFLGES GLENQVKVRQ DQIDPAIAKA 600
    IELENLAAKV KIGKFGPYIE IPQGEEIITA SIPQDLTPAD LNPEQVAVLL 650
    KQKTEGPDKV GIHPETGEPI FILIGAYGPY VQLGEATEEN KKPKRTSLPK 700
    GVKPEDVTLE MAVGLLALPR NLGEHPESGK NIKASLGRFG PYVVHDQGKD 750
    GKDYRSLKGD DDVLTITLER ALELLAEPKR GRGSRTPLKQ LGLHPEDQEP 800
    VNLFKGPYGV YIKHGKVNAS LPEGETEETI TLEKALPLLA EKAGSGKKTS 850
    RKKATTTAKG TKKTTSKKAS ATGTAKKTTT KRTTRKKAAS PDQSSEAG 898
    Length:898
    Mass (Da):99,340
    Last modified:February 1, 1997 - v1
    Checksum:i9416665977398EB3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000022 Genomic DNA. Translation: BAA17864.1.
    PIRiS74903.
    RefSeqiNP_441184.1. NC_000911.1.
    YP_005651241.1. NC_017277.1.
    YP_007451066.1. NC_020286.1.

    Genome annotation databases

    EnsemblBacteriaiBAA17864; BAA17864; BAA17864.
    GeneIDi954520.
    KEGGisyn:slr2058.
    syy:SYNGTS_1288.
    syz:MYO_113000.
    PATRICi23839689. VBISynSp132158_1406.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000022 Genomic DNA. Translation: BAA17864.1 .
    PIRi S74903.
    RefSeqi NP_441184.1. NC_000911.1.
    YP_005651241.1. NC_017277.1.
    YP_007451066.1. NC_020286.1.

    3D structure databases

    ProteinModelPortali P73810.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 1148.slr2058.

    Proteomic databases

    PaxDbi P73810.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA17864 ; BAA17864 ; BAA17864 .
    GeneIDi 954520.
    KEGGi syn:slr2058.
    syy:SYNGTS_1288.
    syz:MYO_113000.
    PATRICi 23839689. VBISynSp132158_1406.

    Phylogenomic databases

    eggNOGi COG1754.
    HOGENOMi HOG000004020.
    KOi K03168.
    OMAi PSCNTGE.
    OrthoDBi EOG6S7XQ9.
    PhylomeDBi P73810.

    Family and domain databases

    Gene3Di 1.10.460.10. 2 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPi MF_00952. Topoisom_1_prok.
    InterProi IPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR005733. TopoI_bac-type.
    IPR028612. Topoisom_1_IA.
    IPR025589. Toprim_C_rpt.
    IPR006171. Toprim_domain.
    [Graphical view ]
    PANTHERi PTHR11390. PTHR11390. 1 hit.
    Pfami PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF13368. Toprim_C_rpt. 3 hits.
    [Graphical view ]
    PRINTSi PR00417. PRTPISMRASEI.
    SMARTi SM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56712. SSF56712. 1 hit.
    TIGRFAMsi TIGR01051. topA_bact. 1 hit.
    PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
      Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
      , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PCC 6803 / Kazusa.

    Entry informationi

    Entry nameiTOP1_SYNY3
    AccessioniPrimary (citable) accession number: P73810
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Synechocystis PCC 6803
      Synechocystis (strain PCC 6803): entries and gene names

    External Data

    Dasty 3