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P73810

- TOP1_SYNY3

UniProt

P73810 - TOP1_SYNY3

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Protein

DNA topoisomerase 1

Gene
topA, slr2058
Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi8 – 81Magnesium 1; catalytic By similarity
Sitei32 – 321Interaction with DNA By similarity
Metal bindingi95 – 951Magnesium 1; catalytic By similarity
Metal bindingi95 – 951Magnesium 2 By similarity
Metal bindingi97 – 971Magnesium 2 By similarity
Sitei151 – 1511Interaction with DNA By similarity
Sitei152 – 1521Interaction with DNA By similarity
Sitei155 – 1551Interaction with DNA By similarity
Sitei160 – 1601Interaction with DNA By similarity
Sitei167 – 1671Interaction with DNA By similarity
Active sitei320 – 3201O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Sitei322 – 3221Interaction with DNA By similarity
Sitei515 – 5151Interaction with DNA By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topA
Ordered Locus Names:slr2058
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 898898DNA topoisomerase 1UniRule annotationPRO_0000145168Add
BLAST

Proteomic databases

PaxDbiP73810.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi1148.slr2058.

Structurei

3D structure databases

ProteinModelPortaliP73810.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 126125ToprimAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni175 – 1806Interaction with DNA By similarity

Sequence similaritiesi

Contains 1 Toprim domain.

Phylogenomic databases

eggNOGiCOG1754.
HOGENOMiHOG000004020.
KOiK03168.
OMAiPSCNTGE.
OrthoDBiEOG6S7XQ9.
PhylomeDBiP73810.

Family and domain databases

Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR025589. Toprim_C_rpt.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF13368. Toprim_C_rpt. 3 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P73810-1 [UniParc]FASTAAdd to Basket

« Hide

MSKLVIVESP TKARTIRNYL PQDYRVEASM GHVRDLPASA EEVPAAYKDK    50
SWANLGVNVE DHFSPLYVIP KSKKKVVKEL QTALKNADEV ILATDEDREG 100
ESISWHLLQL LQPKVPIKRM VFHEITQEAI RSALENCRDI DENLVHAQET 150
RRILDRLVGY TLSPLLWKKI AWGLSAGRVQ SVAVRLIVQR ERARRAFKTA 200
GYWDLKAELE QNKNPFQAKL MTLGGTKLAN GSDFDPNTGA LLPDKQVVVL 250
DEAQAIALKE RLTGKPWAVV NTEEKPGVRK PSPPFTTSTL QQEANRKLGI 300
SARDTMRVAQ KLYEEGYITY MRTDSVHLSD QAVTAARNCV QQMYGKEYLS 350
PQPKQYTTKS KGAQEAHEAI RPAGTEFRIP NQTGLKDREL ALYELIWKRT 400
VACQMADARI TQLSVLLKVE DAEFRAAGKR IDFPGYFRAY VEGSDDPDAA 450
LENQEVILPP LKVGDRPNCR EIDTVGHETQ PPARYTEASL VKTLESEGVG 500
RPSTYASIIG TIIDRGYVQM RSKALTPTFT AFAVVSLLES HFPDLVDTGF 550
TSRMEQKLDE IAIGKTQWLP YLQGFFLGES GLENQVKVRQ DQIDPAIAKA 600
IELENLAAKV KIGKFGPYIE IPQGEEIITA SIPQDLTPAD LNPEQVAVLL 650
KQKTEGPDKV GIHPETGEPI FILIGAYGPY VQLGEATEEN KKPKRTSLPK 700
GVKPEDVTLE MAVGLLALPR NLGEHPESGK NIKASLGRFG PYVVHDQGKD 750
GKDYRSLKGD DDVLTITLER ALELLAEPKR GRGSRTPLKQ LGLHPEDQEP 800
VNLFKGPYGV YIKHGKVNAS LPEGETEETI TLEKALPLLA EKAGSGKKTS 850
RKKATTTAKG TKKTTSKKAS ATGTAKKTTT KRTTRKKAAS PDQSSEAG 898
Length:898
Mass (Da):99,340
Last modified:February 1, 1997 - v1
Checksum:i9416665977398EB3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000022 Genomic DNA. Translation: BAA17864.1.
PIRiS74903.
RefSeqiNP_441184.1. NC_000911.1.
YP_005651241.1. NC_017277.1.
YP_007451066.1. NC_020286.1.

Genome annotation databases

EnsemblBacteriaiBAA17864; BAA17864; BAA17864.
GeneIDi954520.
KEGGisyn:slr2058.
syy:SYNGTS_1288.
syz:MYO_113000.
PATRICi23839689. VBISynSp132158_1406.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000022 Genomic DNA. Translation: BAA17864.1 .
PIRi S74903.
RefSeqi NP_441184.1. NC_000911.1.
YP_005651241.1. NC_017277.1.
YP_007451066.1. NC_020286.1.

3D structure databases

ProteinModelPortali P73810.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 1148.slr2058.

Proteomic databases

PaxDbi P73810.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA17864 ; BAA17864 ; BAA17864 .
GeneIDi 954520.
KEGGi syn:slr2058.
syy:SYNGTS_1288.
syz:MYO_113000.
PATRICi 23839689. VBISynSp132158_1406.

Phylogenomic databases

eggNOGi COG1754.
HOGENOMi HOG000004020.
KOi K03168.
OMAi PSCNTGE.
OrthoDBi EOG6S7XQ9.
PhylomeDBi P73810.

Family and domain databases

Gene3Di 1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00952. Topoisom_1_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR025589. Toprim_C_rpt.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF13368. Toprim_C_rpt. 3 hits.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
TIGRFAMsi TIGR01051. topA_bact. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.

Entry informationi

Entry nameiTOP1_SYNY3
AccessioniPrimary (citable) accession number: P73810
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: September 3, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3

Similar proteinsi