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P73715 (END3_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Endonuclease III

EC=4.2.99.18
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyase
Gene names
Name:nth
Ordered Locus Names:slr1822
OrganismSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifier1111708 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaChroococcalesSynechocystis

Protein attributes

Sequence length219 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site By similarity.

Catalytic activity

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

Cofactor

Binds 1 4Fe-4S cluster. The cluster is not important for the catalytic activity, but which is probably involved in the proper positioning of the enzyme along the DNA strand By similarity.

Sequence similarities

Belongs to the Nth/MutY family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 219219Endonuclease III
PRO_0000102223

Sites

Metal binding1971Iron-sulfur (4Fe-4S) By similarity
Metal binding2041Iron-sulfur (4Fe-4S) By similarity
Metal binding2071Iron-sulfur (4Fe-4S) By similarity
Metal binding2131Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
P73715 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: DB4EF2681C309949

FASTA21924,377
        10         20         30         40         50         60 
MSSLLRKMAS KKQRATEILL ILKKLYPGAT CSLDYQTPVQ LLVATILSAQ CTDERVNKVT 

        70         80         90        100        110        120 
PALFQRYPDA NALAYGDRQE IEELIHSTGF FRNKAKNIQG ACRKIVEEFD GEVPQRMEEL 

       130        140        150        160        170        180 
LTLPGVARKT ANVVLAHAFG ILAGVTVDTH VKRLSQRLGL TKATDPIRIE RDLMKLIPQP 

       190        200        210 
DWENFSIHII YHGRAVCAAR KPLCGECQLA HLCPSAQAS 

« Hide

References

[1]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. expand/collapse author list , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27184 / PCC 6803 / N-1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000022 Genomic DNA. Translation: BAA17762.1.
PIRS77204.
RefSeqNP_441082.1. NC_000911.1.

3D structure databases

ProteinModelPortalP73715.
ModBaseSearch...

Protein-protein interaction databases

STRINGP73715.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID954396.
GenomeReviewsGene locus slr1822 in contig BA000022_GR.
KEGGsyn:slr1822.
NMPDRfig|1148.1.peg.1183.
PATRIC23839458. VBISynSp132158_1293.

Phylogenomic databases

eggNOGCOG0177.
HOGENOMHBG464473.
OMAACGACPV.
PhylomeDBP73715.
ProtClustDBCLSK892970.

Enzyme and pathway databases

BioCycSSP1148:SLR1822-MONOMER.

Family and domain databases

InterProIPR011257. DNA_glycosylase.
IPR004036. Endonuclease-III_CS2.
IPR005759. Endonuclease-III_Nth.
IPR004035. Endouclease-III_FeS-bd_BS.
IPR003651. Endouclease3_FeS-loop_motif.
IPR003265. HhH-GPD_domain.
IPR000445. HhH_motif.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR023170. HTH_base_excis_C.
[Graphical view]
Gene3DG3DSA:1.10.340.30. DNA_glycosylase. 1 hit.
G3DSA:1.10.1670.10. HTH_base_excis_C. 1 hit.
KOK10773.
PfamPF00633. HHH. 1 hit.
PF00730. HhH-GPD. 1 hit.
[Graphical view]
SMARTSM00478. ENDO3c. 1 hit.
SM00525. FES. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view]
SUPFAMSSF48150. DNA_glycsylse. 1 hit.
TIGRFAMsTIGR01083. Nth. 1 hit.
PROSITEPS00764. ENDONUCLEASE_III_1. 1 hit.
PS01155. ENDONUCLEASE_III_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameEND3_SYNY3
AccessionPrimary (citable) accession number: P73715
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: January 25, 2012
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Synechocystis PCC 6803

Synechocystis (strain PCC 6803): entries and gene names

SIMILARITY comments

Index of protein domains and families