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P73572 (LIPA2_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lipoyl synthase 2

EC=2.8.1.8
Alternative name(s):
Lip-syn 2
Lipoate synthase 2
Lipoic acid synthase 2
Sulfur insertion protein lipA2
Gene names
Name:lipA2
Ordered Locus Names:sll0868
OrganismSynechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]
Taxonomic identifier1111708 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis

Protein attributes

Sequence length303 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity. HAMAP-Rule MF_00206

Catalytic activity

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_00206

Cofactor

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathway

Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. HAMAP-Rule MF_00206

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00206.

Sequence similarities

Belongs to the radical SAM superfamily. Lipoyl synthase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   Ligand4Fe-4S
Iron
Iron-sulfur
Metal-binding
S-adenosyl-L-methionine
   Molecular functionTransferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotein lipoylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function4 iron, 4 sulfur cluster binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

lipoate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 303303Lipoyl synthase 2 HAMAP-Rule MF_00206
PRO_0000102372

Sites

Metal binding501Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding551Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding611Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding761Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal binding801Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal binding831Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

Sequences

Sequence LengthMass (Da)Tools
P73572 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 9E259BDC3D262653

FASTA30334,267
        10         20         30         40         50         60 
MPQQSFMNGS SLPREFPPRL PSWLKRPIGR ASELSTVQQI IKQRQIHTIC EEGRCPNRGE 

        70         80         90        100        110        120 
CYANRTATFL LMGQICTRAC GFCQVEKGQA PMMLDQDEPR KVAEAVQLLG LKYVVLTSVA 

       130        140        150        160        170        180 
RDDLTDGGAG WFVAVMERIR QDNPATQIEV LTPDFWGGMG RERSQKERVL TVTKAKPVCY 

       190        200        210        220        230        240 
NHNLETVARL QGKVRRGAKY QRSLDVLRWI KEFDPDIFTK SGLMLGHGET VDEVVETLKD 

       250        260        270        280        290        300 
LRSVGCDRLT LGQYMQPSLD HLPVQKYWTP EEFDQLGEIA KDLGFSHVRS GPLVRSSYHA 


GED 

« Hide

References

[1]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. expand/collapse author list , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000022 Genomic DNA. Translation: BAA17612.1.
PIRS77278.
RefSeqNP_440932.1. NC_000911.1.
YP_005650991.1. NC_017277.1.
YP_007450815.1. NC_020286.1.

3D structure databases

ProteinModelPortalP73572.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING1148.sll0868.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA17612; BAA17612; BAA17612.
GeneID12255077.
14616469.
954238.
KEGGsyn:sll0868.
syy:SYNGTS_1038.
syz:MYO_110470.
PATRIC23839136. VBISynSp132158_1132.

Phylogenomic databases

eggNOGCOG0320.
HOGENOMHOG000235997.
KOK03644.
OMAVQKYWTP.
OrthoDBEOG6038ZS.
PhylomeDBP73572.
ProtClustDBPRK12928.

Enzyme and pathway databases

UniPathwayUPA00538; UER00593.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00206. Lipoyl_synth.
InterProIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERPTHR10949. PTHR10949. 1 hit.
PfamPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF005963. Lipoyl_synth. 1 hit.
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00510. lipA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameLIPA2_SYNY3
AccessionPrimary (citable) accession number: P73572
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: February 1, 1997
Last modified: April 16, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Synechocystis PCC 6803

Synechocystis (strain PCC 6803): entries and gene names

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways