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Protein

Lipoyl synthase 2

Gene

lipA2

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N6-(octanoyl)lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+ = protein N6-(dihydrolipoyl)lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Octanoyltransferase (lipB)
  2. Lipoyl synthase 1 (lipA1), Lipoyl synthase 2 (lipA2)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi50Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi55Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi61Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi76Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi80Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi83Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase 2UniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lip-syn 2UniRule annotation
Lipoate synthase 2UniRule annotation
Lipoic acid synthase 2UniRule annotation
Sulfur insertion protein lipA2
Gene namesi
Name:lipA2UniRule annotation
Ordered Locus Names:sll0868
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesMerismopediaceaeSynechocystis
Proteomesi
  • UP000001425 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001023721 – 303Lipoyl synthase 2Add BLAST303

Proteomic databases

PaxDbiP73572
PRIDEiP73572

Interactioni

Protein-protein interaction databases

STRINGi1148.SYNGTS_1038

Structurei

3D structure databases

ProteinModelPortaliP73572
SMRiP73572
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000235997
InParanoidiP73572
KOiK03644
OMAiGRCPNRG
PhylomeDBiP73572

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR003698 Lipoyl_synth
IPR007197 rSAM
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF005963 Lipoyl_synth, 1 hit
SFLDiSFLDG01058 lipoyl_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit

Sequencei

Sequence statusi: Complete.

P73572-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPQQSFMNGS SLPREFPPRL PSWLKRPIGR ASELSTVQQI IKQRQIHTIC
60 70 80 90 100
EEGRCPNRGE CYANRTATFL LMGQICTRAC GFCQVEKGQA PMMLDQDEPR
110 120 130 140 150
KVAEAVQLLG LKYVVLTSVA RDDLTDGGAG WFVAVMERIR QDNPATQIEV
160 170 180 190 200
LTPDFWGGMG RERSQKERVL TVTKAKPVCY NHNLETVARL QGKVRRGAKY
210 220 230 240 250
QRSLDVLRWI KEFDPDIFTK SGLMLGHGET VDEVVETLKD LRSVGCDRLT
260 270 280 290 300
LGQYMQPSLD HLPVQKYWTP EEFDQLGEIA KDLGFSHVRS GPLVRSSYHA

GED
Length:303
Mass (Da):34,267
Last modified:February 1, 1997 - v1
Checksum:i9E259BDC3D262653
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA Translation: BAA17612.1
PIRiS77278

Genome annotation databases

EnsemblBacteriaiBAA17612; BAA17612; BAA17612
KEGGisyn:sll0868

Similar proteinsi

Entry informationi

Entry nameiLIPA2_SYNY3
AccessioniPrimary (citable) accession number: P73572
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: February 1, 1997
Last modified: May 23, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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