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Protein

Glutamyl-tRNA(Gln) amidotransferase subunit A

Gene

gatA

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) (By similarity).By similarity

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei75 – 751Charge relay systemBy similarity
Active sitei150 – 1501Charge relay systemBy similarity
Active sitei174 – 1741Acyl-ester intermediateBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. translation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit A (EC:6.3.5.7)
Short name:
Glu-ADT subunit A
Gene namesi
Name:gatA
Ordered Locus Names:slr0877
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 483483Glutamyl-tRNA(Gln) amidotransferase subunit APRO_0000105220Add
BLAST

Proteomic databases

PaxDbiP73558.

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.By similarity

Protein-protein interaction databases

IntActiP73558. 3 interactions.
STRINGi1148.slr0877.

Structurei

3D structure databases

ProteinModelPortaliP73558.
SMRiP73558. Positions 4-481.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the amidase family. GatA subfamily.Curated

Phylogenomic databases

eggNOGiCOG0154.
HOGENOMiHOG000116699.
InParanoidiP73558.
KOiK02433.
OMAiKEYFGAG.
OrthoDBiEOG61P6R9.
PhylomeDBiP73558.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P73558-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLIRALHQQ LIDQEISAVA VAQASLARIE AVDDRLKSFL QVTAPQAIAQ
60 70 80 90 100
AEKVDAQIAA GEPIGLLAGI PIGIKDNLCT KGIVTTCASQ ILRGFVPPYE
110 120 130 140 150
STVTEKLQKA GAVMVGKTNL DEFAMGSSTE NSGYQVTANP WDLTRVPGGS
160 170 180 190 200
SGGSAAAVAA DECLIALGSD TGGSIRQPAS LCGVVGMKPT YGLVSRFGLV
210 220 230 240 250
AYASSLDQIG PFARRVEDAA ILLQAIAGHD SRDSTSLDVP IPDYTQALKP
260 270 280 290 300
DLKGVKVGVI MDAFGEGLDD TVNEAVQTAI AKLRELGATI EEIHCPRFRS
310 320 330 340 350
GIAAYYVIAP SEASANLARY DAVRYGLRAE ADNLMEMYTH TRAKGFGAEV
360 370 380 390 400
KRRIMLGTYA LSAGYYDAYY LKAQKVRTLI KQDFDQAFTK VDVLVCPTAP
410 420 430 440 450
TTAFKAGEKT DDPLSMYLSD LMTIPVNLAG LPAMSVPCGF DDNNLPIGLQ
460 470 480
LVGNVLGEEM LFRVGYAYEQ ATTWHDRQPD LSA
Length:483
Mass (Da):51,471
Last modified:February 1, 1997 - v1
Checksum:i2DB78663B9087403
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA17598.1.
PIRiS77264.
RefSeqiNP_440918.1. NC_000911.1.
YP_005650977.1. NC_017277.1.
YP_007450801.1. NC_020286.1.

Genome annotation databases

EnsemblBacteriaiBAA17598; BAA17598; BAA17598.
GeneIDi954223.
KEGGisyn:slr0877.
PATRICi23839102. VBISynSp132158_1115.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA17598.1.
PIRiS77264.
RefSeqiNP_440918.1. NC_000911.1.
YP_005650977.1. NC_017277.1.
YP_007450801.1. NC_020286.1.

3D structure databases

ProteinModelPortaliP73558.
SMRiP73558. Positions 4-481.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP73558. 3 interactions.
STRINGi1148.slr0877.

Proteomic databases

PaxDbiP73558.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA17598; BAA17598; BAA17598.
GeneIDi954223.
KEGGisyn:slr0877.
PATRICi23839102. VBISynSp132158_1115.

Phylogenomic databases

eggNOGiCOG0154.
HOGENOMiHOG000116699.
InParanoidiP73558.
KOiK02433.
OMAiKEYFGAG.
OrthoDBiEOG61P6R9.
PhylomeDBiP73558.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.

Entry informationi

Entry nameiGATA_SYNY3
AccessioniPrimary (citable) accession number: P73558
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 1, 1997
Last modified: January 7, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.