P73558 (GATA_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit A
Short name=Glu-ADT subunit A
EC=6.3.5.-

Gene names

Name:	gatA
Ordered Locus Names:	slr0877

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechocystis › Synechocystis sp. PCC 6803

Protein attributes

Sequence length	483 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) By similarity. HAMAP-Rule MF_00120
Catalytic activity	ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP-Rule MF_00120
Subunit structure	Heterotrimer of A, B and C subunits By similarity.
Sequence similarities	Belongs to the amidase family. GatA subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 483	483	Glutamyl-tRNA(Gln) amidotransferase subunit A HAMAP-Rule MF_00120		PRO_0000105220
Sites
	Active site	75	1	Charge relay system By similarity
	Active site	150	1	Charge relay system By similarity
	Active site	174	1	Acyl-ester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P73558 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 2DB78663B9087403	FASTA	483	51,471
	10 20 30 40 50 60 MSLIRALHQQ LIDQEISAVA VAQASLARIE AVDDRLKSFL QVTAPQAIAQ AEKVDAQIAA 70 80 90 100 110 120 GEPIGLLAGI PIGIKDNLCT KGIVTTCASQ ILRGFVPPYE STVTEKLQKA GAVMVGKTNL 130 140 150 160 170 180 DEFAMGSSTE NSGYQVTANP WDLTRVPGGS SGGSAAAVAA DECLIALGSD TGGSIRQPAS 190 200 210 220 230 240 LCGVVGMKPT YGLVSRFGLV AYASSLDQIG PFARRVEDAA ILLQAIAGHD SRDSTSLDVP 250 260 270 280 290 300 IPDYTQALKP DLKGVKVGVI MDAFGEGLDD TVNEAVQTAI AKLRELGATI EEIHCPRFRS 310 320 330 340 350 360 GIAAYYVIAP SEASANLARY DAVRYGLRAE ADNLMEMYTH TRAKGFGAEV KRRIMLGTYA 370 380 390 400 410 420 LSAGYYDAYY LKAQKVRTLI KQDFDQAFTK VDVLVCPTAP TTAFKAGEKT DDPLSMYLSD 430 440 450 460 470 480 LMTIPVNLAG LPAMSVPCGF DDNNLPIGLQ LVGNVLGEEM LFRVGYAYEQ ATTWHDRQPD LSA

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA17598.1.
PIR	S77264.
RefSeq	NP_440918.1. NC_000911.1. YP_005650977.1. NC_017277.1. YP_007450801.1. NC_020286.1.
3D structure databases
ProteinModelPortal	P73558.
SMR	P73558. Positions 4-481.
ModBase	Search...
Protein-protein interaction databases
IntAct	P73558. 3 interactions.
STRING	1148.slr0877.
Proteomic databases
PaxDb	P73558.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAA17598; BAA17598; BAA17598.
GeneID	12255063. 14616455. 954223.
KEGG	syn:slr0877. syy:SYNGTS_1024.
PATRIC	23839102. VBISynSp132158_1115.
Phylogenomic databases
eggNOG	COG0154.
HOGENOM	HOG000116699.
KO	K02433.
OMA	RYDGVKY.
ProtClustDB	PRK00012.
Family and domain databases
Gene3D	3.90.1300.10. 1 hit.
HAMAP	MF_00120. GatA.
InterPro	IPR000120. Amidase. IPR020556. Amidase_CS. IPR023631. Amidase_dom. IPR004412. GatA. [Graphical view]
PANTHER	PTHR11895. PTHR11895. 1 hit.
Pfam	PF01425. Amidase. 1 hit. [Graphical view]
SUPFAM	SSF75304. Amidase_sig_enz. 1 hit.
TIGRFAMs	TIGR00132. gatA. 1 hit.
PROSITE	PS00571. AMIDASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GATA_SYNY3

Accession

Primary (citable) accession number: P73558

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program