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P73558

- GATA_SYNY3

UniProt

P73558 - GATA_SYNY3

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Protein

Glutamyl-tRNA(Gln) amidotransferase subunit A

Gene
gatA, slr0877
Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) By similarity.UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei75 – 751Charge relay system By similarity
Active sitei150 – 1501Charge relay system By similarity
Active sitei174 – 1741Acyl-ester intermediate By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. translation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit A (EC:6.3.5.7)
Short name:
Glu-ADT subunit A
Gene namesi
Name:gatA
Ordered Locus Names:slr0877
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 483483Glutamyl-tRNA(Gln) amidotransferase subunit AUniRule annotationPRO_0000105220Add
BLAST

Proteomic databases

PaxDbiP73558.

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits By similarity.UniRule annotation

Protein-protein interaction databases

IntActiP73558. 3 interactions.
STRINGi1148.slr0877.

Structurei

3D structure databases

ProteinModelPortaliP73558.
SMRiP73558. Positions 4-481.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0154.
HOGENOMiHOG000116699.
KOiK02433.
OMAiGKPFAEE.
OrthoDBiEOG61P6R9.
PhylomeDBiP73558.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P73558-1 [UniParc]FASTAAdd to Basket

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MSLIRALHQQ LIDQEISAVA VAQASLARIE AVDDRLKSFL QVTAPQAIAQ    50
AEKVDAQIAA GEPIGLLAGI PIGIKDNLCT KGIVTTCASQ ILRGFVPPYE 100
STVTEKLQKA GAVMVGKTNL DEFAMGSSTE NSGYQVTANP WDLTRVPGGS 150
SGGSAAAVAA DECLIALGSD TGGSIRQPAS LCGVVGMKPT YGLVSRFGLV 200
AYASSLDQIG PFARRVEDAA ILLQAIAGHD SRDSTSLDVP IPDYTQALKP 250
DLKGVKVGVI MDAFGEGLDD TVNEAVQTAI AKLRELGATI EEIHCPRFRS 300
GIAAYYVIAP SEASANLARY DAVRYGLRAE ADNLMEMYTH TRAKGFGAEV 350
KRRIMLGTYA LSAGYYDAYY LKAQKVRTLI KQDFDQAFTK VDVLVCPTAP 400
TTAFKAGEKT DDPLSMYLSD LMTIPVNLAG LPAMSVPCGF DDNNLPIGLQ 450
LVGNVLGEEM LFRVGYAYEQ ATTWHDRQPD LSA 483
Length:483
Mass (Da):51,471
Last modified:February 1, 1997 - v1
Checksum:i2DB78663B9087403
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000022 Genomic DNA. Translation: BAA17598.1.
PIRiS77264.
RefSeqiNP_440918.1. NC_000911.1.
YP_005650977.1. NC_017277.1.
YP_007450801.1. NC_020286.1.

Genome annotation databases

EnsemblBacteriaiBAA17598; BAA17598; BAA17598.
GeneIDi954223.
KEGGisyn:slr0877.
syy:SYNGTS_1024.
syz:MYO_110330.
PATRICi23839102. VBISynSp132158_1115.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000022 Genomic DNA. Translation: BAA17598.1 .
PIRi S77264.
RefSeqi NP_440918.1. NC_000911.1.
YP_005650977.1. NC_017277.1.
YP_007450801.1. NC_020286.1.

3D structure databases

ProteinModelPortali P73558.
SMRi P73558. Positions 4-481.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P73558. 3 interactions.
STRINGi 1148.slr0877.

Proteomic databases

PaxDbi P73558.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA17598 ; BAA17598 ; BAA17598 .
GeneIDi 954223.
KEGGi syn:slr0877.
syy:SYNGTS_1024.
syz:MYO_110330.
PATRICi 23839102. VBISynSp132158_1115.

Phylogenomic databases

eggNOGi COG0154.
HOGENOMi HOG000116699.
KOi K02433.
OMAi GKPFAEE.
OrthoDBi EOG61P6R9.
PhylomeDBi P73558.

Family and domain databases

Gene3Di 3.90.1300.10. 1 hit.
HAMAPi MF_00120. GatA.
InterProi IPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view ]
PANTHERi PTHR11895. PTHR11895. 1 hit.
Pfami PF01425. Amidase. 1 hit.
[Graphical view ]
SUPFAMi SSF75304. SSF75304. 1 hit.
TIGRFAMsi TIGR00132. gatA. 1 hit.
PROSITEi PS00571. AMIDASES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.

Entry informationi

Entry nameiGATA_SYNY3
AccessioniPrimary (citable) accession number: P73558
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 1, 1997
Last modified: July 9, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3

Similar proteinsi