P73554 (RUVA_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Holliday junction ATP-dependent DNA helicase RuvA
EC=3.6.4.12

Gene names

Name:	ruvA
Ordered Locus Names:	sll0876

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechocystis › Synechocystis sp. PCC 6803

Protein attributes

Sequence length	211 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB By similarity. HAMAP-Rule MF_00031
Catalytic activity	ATP + H2O = ADP + phosphate. HAMAP-Rule MF_00031
Subunit structure	Forms a complex with RuvB By similarity. HAMAP-Rule MF_00031
Sequence similarities	Belongs to the RuvA family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair SOS response
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA duplex unwinding Inferred from electronic annotation. Source: GOC DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repair Inferred from electronic annotation. Source: UniProtKB-KW SOS response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Holliday junction helicase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW four-way junction helicase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 211	211	Holliday junction ATP-dependent DNA helicase RuvA HAMAP-Rule MF_00031		PRO_0000094699

Sequences

	Sequence		Length	Mass (Da)
	P73554 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 781E1816B8589715	FASTA	211	23,190
	10 20 30 40 50 60 MIQFLQGQVV TVTKNIQNRW FLILSVNGVG YELQVPHSLA QQWTPPPPEP QQVFTHLLVR 70 80 90 100 110 120 QDQIALFGFG RLAERDLFGQ LMGVTGIGAQ LAIALIETLG LEGLVQAVVT GNVKQLCQTP 130 140 150 160 170 180 GVGKKGAERL ALELKTKLSQ WHKLQMGTGE TDSTLPTTAL LEDLEMTLLA LGYTQTEIQQ 190 200 210 AIAMVSQVPD VAQSEDPEVW IRQAIGWLSD H

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA17594.1.
PIR	S77260.
RefSeq	NP_440914.1. NC_000911.1. YP_005650973.1. NC_017277.1. YP_007450797.1. NC_020286.1.
3D structure databases
ProteinModelPortal	P73554.
ModBase	Search...
Protein-protein interaction databases
STRING	1148.sll0876.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAA17594; BAA17594; BAA17594.
GeneID	12255059. 14616451. 954217.
KEGG	syn:sll0876. syy:SYNGTS_1020.
PATRIC	23839092. VBISynSp132158_1110.
Phylogenomic databases
eggNOG	COG0632.
HOGENOM	HOG000057116.
KO	K03550.
OMA	MSADEYI.
ProtClustDB	PRK00116.
Family and domain databases
Gene3D	2.40.50.140. 1 hit.
HAMAP	MF_00031. DNA_helic_RuvA.
InterPro	IPR011114. DNA_helicas_Holl-junc_RuvA_C. IPR013849. DNA_helicase_Holl-junc_RuvA_I. IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif. IPR012340. NA-bd_OB-fold. IPR000085. RuvA. IPR010994. RuvA_2-like. [Graphical view]
Pfam	PF07499. RuvA_C. 1 hit. PF01330. RuvA_N. 1 hit. [Graphical view]
SMART	SM00278. HhH1. 2 hits. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF47781. RuvA_2_like. 1 hit.
TIGRFAMs	TIGR00084. ruvA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RUVA_SYNY3

Accession

Primary (citable) accession number: P73554

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 1, 1997
Last modified:	May 29, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program