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P73538

- BIOB_SYNY3

UniProt

P73538 - BIOB_SYNY3

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Protein

Biotin synthase

Gene

bioB

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi88 – 881Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi92 – 921Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi95 – 951Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi133 – 1331Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi170 – 1701Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi230 – 2301Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi300 – 3001Iron-sulfur 2 (2Fe-2S)UniRule annotation

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:slr1364
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 362362Biotin synthasePRO_0000185563Add
BLAST

Proteomic databases

PRIDEiP73538.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

IntActiP73538. 4 interactions.
STRINGi1148.slr1364.

Structurei

3D structure databases

ProteinModelPortaliP73538.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
InParanoidiP73538.
KOiK01012.
OMAiTCENTLR.
OrthoDBiEOG622PMP.
PhylomeDBiP73538.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

P73538-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVLASSRPPS PTNSPADRQG TLQAWLTGLS QRIIDGDRLT REEALQLAAI
60 70 80 90 100
EGEENILLLC EAANRIREAC CGNVVDLCSI INVKSGNCSE NCGFCSQSSH
110 120 130 140 150
HPDPNSPIYG LKTEAEILEQ ARAAAAAGAK RFCLVSQGRG PKYHSPKDRE
160 170 180 190 200
FEQILATVRQ IIQETNIKPC CALGEVTPEQ AQQLKEAGVT RYNHNLEASA
210 220 230 240 250
TYYDKIVSTH TWQDRVDTVK NLKAAGIQAC TGGILGMGET WEDRIDLALA
260 270 280 290 300
LRELEVESVP LNLLNPRPGT PLGEQQKLNP YDALKAIAIF RFILPQQIIR
310 320 330 340 350
YAGGREAVMG ELQDLGLKAG INAMLVGHYL TTLGQPPERD QKLLASLGLE
360
GGEAPIPGEY QS
Length:362
Mass (Da):39,350
Last modified:February 1, 1997 - v1
Checksum:iBF7A16FB1CBC68B4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000022 Genomic DNA. Translation: BAA17578.1.
PIRiS77244.
RefSeqiNP_440898.1. NC_000911.1.
YP_005650957.1. NC_017277.1.
YP_007450781.1. NC_020286.1.

Genome annotation databases

EnsemblBacteriaiBAA17578; BAA17578; BAA17578.
GeneIDi954201.
KEGGisyn:slr1364.
syy:SYNGTS_1004.
syz:MYO_110130.
PATRICi23839056. VBISynSp132158_1092.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000022 Genomic DNA. Translation: BAA17578.1 .
PIRi S77244.
RefSeqi NP_440898.1. NC_000911.1.
YP_005650957.1. NC_017277.1.
YP_007450781.1. NC_020286.1.

3D structure databases

ProteinModelPortali P73538.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P73538. 4 interactions.
STRINGi 1148.slr1364.

Proteomic databases

PRIDEi P73538.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA17578 ; BAA17578 ; BAA17578 .
GeneIDi 954201.
KEGGi syn:slr1364.
syy:SYNGTS_1004.
syz:MYO_110130.
PATRICi 23839056. VBISynSp132158_1092.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
InParanoidi P73538.
KOi K01012.
OMAi TCENTLR.
OrthoDBi EOG622PMP.
PhylomeDBi P73538.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.

Entry informationi

Entry nameiBIOB_SYNY3
AccessioniPrimary (citable) accession number: P73538
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: October 29, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3