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P73538 (BIOB_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:slr1364
OrganismSynechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]
Taxonomic identifier1111708 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis

Protein attributes

Sequence length362 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 362362Biotin synthase HAMAP-Rule MF_01694
PRO_0000185563

Sites

Metal binding881Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding921Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding951Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1331Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1701Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2301Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding3001Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
P73538 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: BF7A16FB1CBC68B4

FASTA36239,350
        10         20         30         40         50         60 
MVLASSRPPS PTNSPADRQG TLQAWLTGLS QRIIDGDRLT REEALQLAAI EGEENILLLC 

        70         80         90        100        110        120 
EAANRIREAC CGNVVDLCSI INVKSGNCSE NCGFCSQSSH HPDPNSPIYG LKTEAEILEQ 

       130        140        150        160        170        180 
ARAAAAAGAK RFCLVSQGRG PKYHSPKDRE FEQILATVRQ IIQETNIKPC CALGEVTPEQ 

       190        200        210        220        230        240 
AQQLKEAGVT RYNHNLEASA TYYDKIVSTH TWQDRVDTVK NLKAAGIQAC TGGILGMGET 

       250        260        270        280        290        300 
WEDRIDLALA LRELEVESVP LNLLNPRPGT PLGEQQKLNP YDALKAIAIF RFILPQQIIR 

       310        320        330        340        350        360 
YAGGREAVMG ELQDLGLKAG INAMLVGHYL TTLGQPPERD QKLLASLGLE GGEAPIPGEY 


QS 

« Hide

References

[1]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. expand/collapse author list , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000022 Genomic DNA. Translation: BAA17578.1.
PIRS77244.
RefSeqNP_440898.1. NC_000911.1.
YP_005650957.1. NC_017277.1.
YP_007450781.1. NC_020286.1.

3D structure databases

ProteinModelPortalP73538.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP73538. 4 interactions.
STRING1148.slr1364.

Proteomic databases

PRIDEP73538.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA17578; BAA17578; BAA17578.
GeneID954201.
KEGGsyn:slr1364.
syy:SYNGTS_1004.
syz:MYO_110130.
PATRIC23839056. VBISynSp132158_1092.

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMATCENTLR.
OrthoDBEOG622PMP.
PhylomeDBP73538.

Enzyme and pathway databases

UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_SYNY3
AccessionPrimary (citable) accession number: P73538
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: July 9, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Synechocystis PCC 6803

Synechocystis (strain PCC 6803): entries and gene names

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways