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Protein

Pyruvate kinase 2

Gene

pyk2

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

Pathway: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 1 (gap1), Glyceraldehyde-3-phosphate dehydrogenase 2 (gap2)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI)
  4. Enolase (eno)
  5. Pyruvate kinase 1 (pyk1), Pyruvate kinase 2 (pyk2)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei38 – 381SubstrateBy similarity
Metal bindingi40 – 401PotassiumBy similarity
Metal bindingi42 – 421PotassiumBy similarity
Metal bindingi72 – 721PotassiumBy similarity
Sitei227 – 2271Transition state stabilizerBy similarity
Metal bindingi229 – 2291MagnesiumBy similarity
Binding sitei252 – 2521Substrate; via amide nitrogenBy similarity
Metal bindingi253 – 2531MagnesiumBy similarity
Binding sitei253 – 2531Substrate; via amide nitrogenBy similarity
Binding sitei285 – 2851SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase 2 (EC:2.7.1.40)
Short name:
PK 2
Gene namesi
Name:pyk2
Ordered Locus Names:sll1275
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 591591Pyruvate kinase 2PRO_0000112086Add
BLAST

Proteomic databases

PaxDbiP73534.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

IntActiP73534. 2 interactions.
STRINGi1148.SYNGTS_1000.

Structurei

3D structure databases

ProteinModelPortaliP73534.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated
In the C-terminal section; belongs to the PEP-utilizing enzyme family.Curated

Phylogenomic databases

eggNOGiCOG0469.
HOGENOMiHOG000021558.
InParanoidiP73534.
KOiK00873.
OMAiKIVAYCD.
OrthoDBiEOG6GBMB0.
PhylomeDBiP73534.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
3.50.30.10. 1 hit.
InterProiIPR008279. PEP-util_enz_mobile_dom.
IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00391. PEP-utilizers. 1 hit.
PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52009. SSF52009. 1 hit.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P73534-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQTSPLPRRT KIVATIGPAT QSKEVLRQLI QAGATTFRLN FSHGDHAYHQ
60 70 80 90 100
QSIRLIRQIA FELNQPVGIL QDLQGPKIRV GKFLNDAGSV QLKNGDPYTL
110 120 130 140 150
TSRPVECTET ISSISYEYLA DEVPSGARIL LDDGKLEMLV EEVDTVARDL
160 170 180 190 200
HCRVIVGGTL SSNKGVNFPG VCLSVKAMTD KDKEDLMFGL DQGVDWVALS
210 220 230 240 250
FVRNPQDIDE IKGLIAAAGK SVPVIAKIEK HEAIKDMQAV LEKCDGVMVA
260 270 280 290 300
RGDLGVELPA EDVPILQKKL IATANRLGIP VITATQMLDS MVNSPRPTRA
310 320 330 340 350
EVSDVANAIL DGTDAVMLSN ETAIGKFPVE AVAIMAKIAE RIEQEDINPS
360 370 380 390 400
QAEASRTSIP NAISSAVSQI AETLNAAAIM SLTKTGSTAR HVSKFRPKTP
410 420 430 440 450
ILAVTPHVDV SRQLQLVWGV KPLLVLDLPS TSQTFQAAIN VAQENHFLRD
460 470 480 490 500
GDLVVMTAGT LQGVAGSTDL IKVEVVKAIL GRGVGIGQGA VSGRARVASR
510 520 530 540 550
PQAIAQFTQG EILVVPSTNA DCVDMMRRAA GIITEEESLT SHAAIIGLRL
560 570 580 590
GVPVIVGFKG ATQKIRDGAI VTIDAQKGLI YSGALPPVSK G
Length:591
Mass (Da):62,986
Last modified:February 1, 1997 - v1
Checksum:i2520AA81145C11EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA17574.1.
PIRiS77240.
RefSeqiYP_005650953.1. NC_017277.1.
YP_007450777.1. NC_020286.1.

Genome annotation databases

EnsemblBacteriaiBAA17574; BAA17574; BAA17574.
KEGGisyn:sll1275.
PATRICi23839046. VBISynSp132158_1087.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA. Translation: BAA17574.1.
PIRiS77240.
RefSeqiYP_005650953.1. NC_017277.1.
YP_007450777.1. NC_020286.1.

3D structure databases

ProteinModelPortaliP73534.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP73534. 2 interactions.
STRINGi1148.SYNGTS_1000.

Proteomic databases

PaxDbiP73534.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA17574; BAA17574; BAA17574.
KEGGisyn:sll1275.
PATRICi23839046. VBISynSp132158_1087.

Phylogenomic databases

eggNOGiCOG0469.
HOGENOMiHOG000021558.
InParanoidiP73534.
KOiK00873.
OMAiKIVAYCD.
OrthoDBiEOG6GBMB0.
PhylomeDBiP73534.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
3.50.30.10. 1 hit.
InterProiIPR008279. PEP-util_enz_mobile_dom.
IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00391. PEP-utilizers. 1 hit.
PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52009. SSF52009. 1 hit.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.

Entry informationi

Entry nameiKPYK2_SYNY3
AccessioniPrimary (citable) accession number: P73534
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: June 24, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.