ID   SPKE_SYNY3              Reviewed;         614 AA.
AC   P73515;
DT   14-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   27-MAR-2024, entry version 132.
DE   RecName: Full=Serine/threonine-protein kinase-like protein E;
GN   Name=spkE; OrderedLocusNames=slr1443;
OS   Synechocystis sp. (strain PCC 6803 / Kazusa).
OC   Bacteria; Cyanobacteriota; Cyanophyceae; Synechococcales; Merismopediaceae;
OC   Synechocystis.
OX   NCBI_TaxID=1111708;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND LACK OF KINASE ACTIVITY.
RX   PubMed=12168951; DOI=10.1093/dnares/9.3.71;
RA   Kamei A., Yuasa T., Geng X., Ikeuchi M.;
RT   "Biochemical examination of the potential eukaryotic-type protein kinase
RT   genes in the complete genome of the unicellular Cyanobacterium
RT   synechocystis sp. PCC 6803.";
RL   DNA Res. 9:71-78(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 6803 / Kazusa;
RX   PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA   Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA   Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA   Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA   Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence analysis of the genome of the unicellular cyanobacterium
RT   Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT   genome and assignment of potential protein-coding regions.";
RL   DNA Res. 3:109-136(1996).
CC   -!- FUNCTION: Lacks protein kinase activity.
CC   -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein
CC       kinase family. {ECO:0000255|PROSITE-ProRule:PRU00159}.
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DR   EMBL; AB046602; BAB17038.1; -; Genomic_DNA.
DR   EMBL; BA000022; BAA17555.1; -; Genomic_DNA.
DR   PIR; S77221; S77221.
DR   AlphaFoldDB; P73515; -.
DR   SMR; P73515; -.
DR   IntAct; P73515; 4.
DR   STRING; 1148.gene:10498420; -.
DR   PaxDb; 1148-1652635; -.
DR   EnsemblBacteria; BAA17555; BAA17555; BAA17555.
DR   KEGG; syn:slr1443; -.
DR   eggNOG; COG0515; Bacteria.
DR   InParanoid; P73515; -.
DR   BRENDA; 2.7.11.1; 382.
DR   Proteomes; UP000001425; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004674; F:protein serine/threonine kinase activity; IBA:GO_Central.
DR   Gene3D; 1.10.510.10; Transferase(Phosphotransferase) domain 1; 1.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   InterPro; IPR000719; Prot_kinase_dom.
DR   PANTHER; PTHR24363; SERINE/THREONINE PROTEIN KINASE; 1.
DR   PANTHER; PTHR24363:SF0; SERINE_THREONINE-PROTEIN KINASE DDB_G0277989-RELATED; 1.
DR   Pfam; PF00069; Pkinase; 1.
DR   SMART; SM00220; S_TKc; 1.
DR   SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR   PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..614
FT                   /note="Serine/threonine-protein kinase-like protein E"
FT                   /id="PRO_0000171243"
FT   DOMAIN          15..404
FT                   /note="Protein kinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT   REGION          256..284
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         21..29
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT   SITE            44
FT                   /note="Defective ATP-binding"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   614 AA;  68700 MW;  19B4F1DB691E96DB CRC64;
     MTPSLSLGQR LDDRYLIQLH LGQNSLGQQY LAQDTHRFDE PCTITVLTAP PGDKAGNLFK
     QQAEILYALD HPQIANFREF FALGSELYLV QDFIEGQSYF DLLKDRRLQG KAFTELEVRQ
     WCRQLLPVLH YLHIQKICHG QISPSSIMRR TVDGLPVLVD FTHSQYYSGT PGEDRRDLHG
     LGLGAIALLT GEVNPNPPWE NLLSSVALSQ EFRQLLLKLL AWPPLVDWPT LTDNLNNLPQ
     HFLPTVAEFN LTSTIPEQTD NGVGKSSTGE PPFPTVHQSP ESSSSAKVKN MVRVDGLKGL
     VKKSFILLGL MAIAMALGWG AGQLWLDNQQ RAALEKLAQE EPGDDVPEKT DLEIKNEIRA
     RRLNLGISPQ RFQTLMDDGL AFQLGIDPQQ RLEKNPNTNG APLSLGSPEQ QMAMTITVLD
     ALESLSREAT RDFAQNNSGD RRRWIPRVNQ LRLSSRSFYD LVNARFHHYL PMVSPALLGE
     PEFEQRPLAQ VWNAMAFDSL ASLEDESHYQ RLSFDDTNQL NLNGTLEPGE GYAYAVTIPP
     TEEFSLQLTA PPTARISFYP PTGPEVILQN SAMHRWSGPT DQAGYYELVI TSVAEEPIAF
     ELELSIIPAS AQPF
//