P73515 (SPKE_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase-like protein E

Gene names

Name:	spkE
Ordered Locus Names:	slr1443

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechocystis › Synechocystis sp. PCC 6803

Protein attributes

Sequence length	614 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Lacks protein kinase activity.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 614	614	Serine/threonine-protein kinase-like protein E		PRO_0000171243
Regions
	Domain	15 – 404	390	Protein kinase
	Nucleotide binding	21 – 29	9	ATP By similarity
Sites
	Site	44	1	Defective ATP-binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	P73515 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 19B4F1DB691E96DB	FASTA	614	68,700
	10 20 30 40 50 60 MTPSLSLGQR LDDRYLIQLH LGQNSLGQQY LAQDTHRFDE PCTITVLTAP PGDKAGNLFK 70 80 90 100 110 120 QQAEILYALD HPQIANFREF FALGSELYLV QDFIEGQSYF DLLKDRRLQG KAFTELEVRQ 130 140 150 160 170 180 WCRQLLPVLH YLHIQKICHG QISPSSIMRR TVDGLPVLVD FTHSQYYSGT PGEDRRDLHG 190 200 210 220 230 240 LGLGAIALLT GEVNPNPPWE NLLSSVALSQ EFRQLLLKLL AWPPLVDWPT LTDNLNNLPQ 250 260 270 280 290 300 HFLPTVAEFN LTSTIPEQTD NGVGKSSTGE PPFPTVHQSP ESSSSAKVKN MVRVDGLKGL 310 320 330 340 350 360 VKKSFILLGL MAIAMALGWG AGQLWLDNQQ RAALEKLAQE EPGDDVPEKT DLEIKNEIRA 370 380 390 400 410 420 RRLNLGISPQ RFQTLMDDGL AFQLGIDPQQ RLEKNPNTNG APLSLGSPEQ QMAMTITVLD 430 440 450 460 470 480 ALESLSREAT RDFAQNNSGD RRRWIPRVNQ LRLSSRSFYD LVNARFHHYL PMVSPALLGE 490 500 510 520 530 540 PEFEQRPLAQ VWNAMAFDSL ASLEDESHYQ RLSFDDTNQL NLNGTLEPGE GYAYAVTIPP 550 560 570 580 590 600 TEEFSLQLTA PPTARISFYP PTGPEVILQN SAMHRWSGPT DQAGYYELVI TSVAEEPIAF 610 ELELSIIPAS AQPF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Biochemical examination of the potential eukaryotic-type protein kinase genes in the complete genome of the unicellular Cyanobacterium synechocystis sp. PCC 6803." Kamei A., Yuasa T., Geng X., Ikeuchi M. DNA Res. 9:71-78(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], LACK OF KINASE ACTIVITY.
[2]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB046602 Genomic DNA. Translation: BAB17038.1. BA000022 Genomic DNA. Translation: BAA17555.1.
PIR	S77221.
RefSeq	NP_440875.1. NC_000911.1. YP_005650934.1. NC_017277.1. YP_007450758.1. NC_020286.1.
3D structure databases
ProteinModelPortal	P73515.
ModBase	Search...
Protein-protein interaction databases
IntAct	P73515. 4 interactions.
STRING	1148.slr1443.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAA17555; BAA17555; BAA17555.
GeneID	12255018. 14616410. 954178.
KEGG	syn:slr1443. syy:SYNGTS_0981.
PATRIC	23839000. VBISynSp132158_1065.
Phylogenomic databases
eggNOG	COG0515.
KO	K08884.
OMA	LEDESHY.
Family and domain databases
InterPro	IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. [Graphical view]
PANTHER	PTHR24347. PTHR24347. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPKE_SYNY3

Accession

Primary (citable) accession number: P73515

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 14, 2001
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program