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P73505

- SYI_SYNY3

UniProt

P73505 - SYI_SYNY3

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei570 – 5701Aminoacyl-adenylateUniRule annotation
    Binding sitei614 – 6141ATPUniRule annotation
    Metal bindingi957 – 9571ZincUniRule annotation
    Metal bindingi960 – 9601ZincUniRule annotation
    Metal bindingi977 – 9771ZincUniRule annotation
    Metal bindingi980 – 9801ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:sll1362
    OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
    Taxonomic identifieri1111708 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
    ProteomesiUP000001425: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 988988Isoleucine--tRNA ligasePRO_0000098492Add
    BLAST

    Proteomic databases

    PaxDbiP73505.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    IntActiP73505. 1 interaction.
    STRINGi1148.sll1362.

    Structurei

    3D structure databases

    ProteinModelPortaliP73505.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi60 – 7011"HIGH" regionAdd
    BLAST
    Motifi611 – 6155"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiLGHRVHY.
    OrthoDBiEOG644ZM1.
    PhylomeDBiP73505.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P73505-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTEAKSYKDT VNLPQTRFDM RANANKREPE IQQYWQEKGI YADLADNNPK    50
    DLFVLHDGPP YANGALHMGH ALNKVLKDII NKYKLLQGHK VHYVPGWDCH 100
    GLPIELKVLQ SLKSSERAEL TPLTLRHKAR DFALKAQQEQ AVGFQRYGIW 150
    GDWKKPYLTL TPEYEAAQIG VFGAMALKGY IYRGLKPVHW SPSSRTALAE 200
    AELEYPEGHT SRSIYVSFPI TQAGEKAAEI LAPYIDDLAV AIWTTTPWTL 250
    PGNLAVALNP ELTYAVVETE SHIFHRSYLI VALDLVEKLS ETFGVKLTVK 300
    VTLQGESLEN TCYQHPLFDR VSPIVIGGDY VTTESGTGLV HTAPGHGQED 350
    YVVGQRYGLP ILSPVDAAGN LTEEAGKFAG LNVLNDANEA IINALQDQKV 400
    LLKEEAYEHK YPYDWRTKKP TIFRATEQWF ASVEGFRDQA LKAIKEVTWI 450
    PTQGENRITP MVGDRSDWCI SRQRAWGVPI PVFYDEETSE PLLTEETINH 500
    VQQIIAEKGS DAWWELTVEE LLPEAYRNNG RTYRKGEDTM DVWFDSGSSW 550
    AAVANAKNRP LKYPVDMYLE GSDQHRGWFQ SSLLTSVAVN GIAPYKTVLT 600
    HGFVLDEKGH KMSKSLGNVV DPYQIINGGK NQKQEPAYGA DVLRLWVASV 650
    DYANDVPIGQ GILKQLVDIR NKIRNTARFL LGNLHDFNPE TDAVAYEDLP 700
    ELDRYMLHRM TEVFTEVTEA YESFQFFKFF QTVQNFCVVD LSNFYLDIAK 750
    DRLYISAPNA PRRRSCQTIL QLALENLTKA IAPVLCHLAE DIWQFLPYEK 800
    PTESVFQSGW VNLDAQWQQP QLAQKWVSLR QLRDGINQLL EQARRDKVIG 850
    SSLEAKLIIV DPRTAESGTD EKQGENFVHW FYGWLENFNE PTFDPNDRKI 900
    SLRNIFLTSQ VEFIKSAEIE PSFTYEETVV INLERHDNNI RTKPIKIIVT 950
    KADGHKCERC WNYSTQVGSF SDDPTICERC NEALQGNF 988
    Length:988
    Mass (Da):112,346
    Last modified:February 1, 1997 - v1
    Checksum:i13423806C0E4F0A0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000022 Genomic DNA. Translation: BAA17545.1.
    PIRiS77211.
    RefSeqiNP_440865.1. NC_000911.1.
    YP_005650924.1. NC_017277.1.
    YP_007450748.1. NC_020286.1.

    Genome annotation databases

    EnsemblBacteriaiBAA17545; BAA17545; BAA17545.
    GeneIDi954168.
    KEGGisyn:sll1362.
    syy:SYNGTS_0971.
    syz:MYO_19780.
    PATRICi23838974. VBISynSp132158_1052.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000022 Genomic DNA. Translation: BAA17545.1 .
    PIRi S77211.
    RefSeqi NP_440865.1. NC_000911.1.
    YP_005650924.1. NC_017277.1.
    YP_007450748.1. NC_020286.1.

    3D structure databases

    ProteinModelPortali P73505.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P73505. 1 interaction.
    STRINGi 1148.sll1362.

    Proteomic databases

    PaxDbi P73505.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA17545 ; BAA17545 ; BAA17545 .
    GeneIDi 954168.
    KEGGi syn:sll1362.
    syy:SYNGTS_0971.
    syz:MYO_19780.
    PATRICi 23838974. VBISynSp132158_1052.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi LGHRVHY.
    OrthoDBi EOG644ZM1.
    PhylomeDBi P73505.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
      Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
      , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PCC 6803 / Kazusa.

    Entry informationi

    Entry nameiSYI_SYNY3
    AccessioniPrimary (citable) accession number: P73505
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Synechocystis PCC 6803
      Synechocystis (strain PCC 6803): entries and gene names

    External Data

    Dasty 3