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P73505

- SYI_SYNY3

UniProt

P73505 - SYI_SYNY3

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Protein
Isoleucine--tRNA ligase
Gene
ileS, sll1362
Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei570 – 5701Aminoacyl-adenylate By similarity
Binding sitei614 – 6141ATP By similarity
Metal bindingi957 – 9571Zinc By similarity
Metal bindingi960 – 9601Zinc By similarity
Metal bindingi977 – 9771Zinc By similarity
Metal bindingi980 – 9801Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:sll1362
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 988988Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098492Add
BLAST

Proteomic databases

PaxDbiP73505.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

IntActiP73505. 1 interaction.
STRINGi1148.sll1362.

Structurei

3D structure databases

ProteinModelPortaliP73505.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi60 – 7011"HIGH" regionUniRule annotation
Add
BLAST
Motifi611 – 6155"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiLGHRVHY.
OrthoDBiEOG644ZM1.
PhylomeDBiP73505.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P73505-1 [UniParc]FASTAAdd to Basket

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MTEAKSYKDT VNLPQTRFDM RANANKREPE IQQYWQEKGI YADLADNNPK    50
DLFVLHDGPP YANGALHMGH ALNKVLKDII NKYKLLQGHK VHYVPGWDCH 100
GLPIELKVLQ SLKSSERAEL TPLTLRHKAR DFALKAQQEQ AVGFQRYGIW 150
GDWKKPYLTL TPEYEAAQIG VFGAMALKGY IYRGLKPVHW SPSSRTALAE 200
AELEYPEGHT SRSIYVSFPI TQAGEKAAEI LAPYIDDLAV AIWTTTPWTL 250
PGNLAVALNP ELTYAVVETE SHIFHRSYLI VALDLVEKLS ETFGVKLTVK 300
VTLQGESLEN TCYQHPLFDR VSPIVIGGDY VTTESGTGLV HTAPGHGQED 350
YVVGQRYGLP ILSPVDAAGN LTEEAGKFAG LNVLNDANEA IINALQDQKV 400
LLKEEAYEHK YPYDWRTKKP TIFRATEQWF ASVEGFRDQA LKAIKEVTWI 450
PTQGENRITP MVGDRSDWCI SRQRAWGVPI PVFYDEETSE PLLTEETINH 500
VQQIIAEKGS DAWWELTVEE LLPEAYRNNG RTYRKGEDTM DVWFDSGSSW 550
AAVANAKNRP LKYPVDMYLE GSDQHRGWFQ SSLLTSVAVN GIAPYKTVLT 600
HGFVLDEKGH KMSKSLGNVV DPYQIINGGK NQKQEPAYGA DVLRLWVASV 650
DYANDVPIGQ GILKQLVDIR NKIRNTARFL LGNLHDFNPE TDAVAYEDLP 700
ELDRYMLHRM TEVFTEVTEA YESFQFFKFF QTVQNFCVVD LSNFYLDIAK 750
DRLYISAPNA PRRRSCQTIL QLALENLTKA IAPVLCHLAE DIWQFLPYEK 800
PTESVFQSGW VNLDAQWQQP QLAQKWVSLR QLRDGINQLL EQARRDKVIG 850
SSLEAKLIIV DPRTAESGTD EKQGENFVHW FYGWLENFNE PTFDPNDRKI 900
SLRNIFLTSQ VEFIKSAEIE PSFTYEETVV INLERHDNNI RTKPIKIIVT 950
KADGHKCERC WNYSTQVGSF SDDPTICERC NEALQGNF 988
Length:988
Mass (Da):112,346
Last modified:February 1, 1997 - v1
Checksum:i13423806C0E4F0A0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000022 Genomic DNA. Translation: BAA17545.1.
PIRiS77211.
RefSeqiNP_440865.1. NC_000911.1.
YP_005650924.1. NC_017277.1.
YP_007450748.1. NC_020286.1.

Genome annotation databases

EnsemblBacteriaiBAA17545; BAA17545; BAA17545.
GeneIDi954168.
KEGGisyn:sll1362.
syy:SYNGTS_0971.
syz:MYO_19780.
PATRICi23838974. VBISynSp132158_1052.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000022 Genomic DNA. Translation: BAA17545.1 .
PIRi S77211.
RefSeqi NP_440865.1. NC_000911.1.
YP_005650924.1. NC_017277.1.
YP_007450748.1. NC_020286.1.

3D structure databases

ProteinModelPortali P73505.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P73505. 1 interaction.
STRINGi 1148.sll1362.

Proteomic databases

PaxDbi P73505.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA17545 ; BAA17545 ; BAA17545 .
GeneIDi 954168.
KEGGi syn:sll1362.
syy:SYNGTS_0971.
syz:MYO_19780.
PATRICi 23838974. VBISynSp132158_1052.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi LGHRVHY.
OrthoDBi EOG644ZM1.
PhylomeDBi P73505.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.

Entry informationi

Entry nameiSYI_SYNY3
AccessioniPrimary (citable) accession number: P73505
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: July 9, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3

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