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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei570Aminoacyl-adenylateUniRule annotation1
Binding sitei614ATPUniRule annotation1
Metal bindingi957ZincUniRule annotation1
Metal bindingi960ZincUniRule annotation1
Metal bindingi977ZincUniRule annotation1
Metal bindingi980ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:sll1362
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesMerismopediaceaeSynechocystis
Proteomesi
  • UP000001425 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000984921 – 988Isoleucine--tRNA ligaseAdd BLAST988

Proteomic databases

PaxDbiP73505
PRIDEiP73505

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

IntActiP73505, 1 interactor
STRINGi1148.SYNGTS_0971

Structurei

3D structure databases

ProteinModelPortaliP73505
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi60 – 70"HIGH" regionAdd BLAST11
Motifi611 – 615"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000246402
InParanoidiP73505
KOiK01870
OMAiHLGTAWN
PhylomeDBiP73505

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

P73505-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEAKSYKDT VNLPQTRFDM RANANKREPE IQQYWQEKGI YADLADNNPK
60 70 80 90 100
DLFVLHDGPP YANGALHMGH ALNKVLKDII NKYKLLQGHK VHYVPGWDCH
110 120 130 140 150
GLPIELKVLQ SLKSSERAEL TPLTLRHKAR DFALKAQQEQ AVGFQRYGIW
160 170 180 190 200
GDWKKPYLTL TPEYEAAQIG VFGAMALKGY IYRGLKPVHW SPSSRTALAE
210 220 230 240 250
AELEYPEGHT SRSIYVSFPI TQAGEKAAEI LAPYIDDLAV AIWTTTPWTL
260 270 280 290 300
PGNLAVALNP ELTYAVVETE SHIFHRSYLI VALDLVEKLS ETFGVKLTVK
310 320 330 340 350
VTLQGESLEN TCYQHPLFDR VSPIVIGGDY VTTESGTGLV HTAPGHGQED
360 370 380 390 400
YVVGQRYGLP ILSPVDAAGN LTEEAGKFAG LNVLNDANEA IINALQDQKV
410 420 430 440 450
LLKEEAYEHK YPYDWRTKKP TIFRATEQWF ASVEGFRDQA LKAIKEVTWI
460 470 480 490 500
PTQGENRITP MVGDRSDWCI SRQRAWGVPI PVFYDEETSE PLLTEETINH
510 520 530 540 550
VQQIIAEKGS DAWWELTVEE LLPEAYRNNG RTYRKGEDTM DVWFDSGSSW
560 570 580 590 600
AAVANAKNRP LKYPVDMYLE GSDQHRGWFQ SSLLTSVAVN GIAPYKTVLT
610 620 630 640 650
HGFVLDEKGH KMSKSLGNVV DPYQIINGGK NQKQEPAYGA DVLRLWVASV
660 670 680 690 700
DYANDVPIGQ GILKQLVDIR NKIRNTARFL LGNLHDFNPE TDAVAYEDLP
710 720 730 740 750
ELDRYMLHRM TEVFTEVTEA YESFQFFKFF QTVQNFCVVD LSNFYLDIAK
760 770 780 790 800
DRLYISAPNA PRRRSCQTIL QLALENLTKA IAPVLCHLAE DIWQFLPYEK
810 820 830 840 850
PTESVFQSGW VNLDAQWQQP QLAQKWVSLR QLRDGINQLL EQARRDKVIG
860 870 880 890 900
SSLEAKLIIV DPRTAESGTD EKQGENFVHW FYGWLENFNE PTFDPNDRKI
910 920 930 940 950
SLRNIFLTSQ VEFIKSAEIE PSFTYEETVV INLERHDNNI RTKPIKIIVT
960 970 980
KADGHKCERC WNYSTQVGSF SDDPTICERC NEALQGNF
Length:988
Mass (Da):112,346
Last modified:February 1, 1997 - v1
Checksum:i13423806C0E4F0A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA Translation: BAA17545.1
PIRiS77211

Genome annotation databases

EnsemblBacteriaiBAA17545; BAA17545; BAA17545
KEGGisyn:sll1362

Similar proteinsi

Entry informationi

Entry nameiSYI_SYNY3
AccessioniPrimary (citable) accession number: P73505
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: May 23, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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