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P73505 (SYI_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:ileS
Ordered Locus Names:sll1362
OrganismSynechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]
Taxonomic identifier1111708 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis

Protein attributes

Sequence length988 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02002

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_02002

Subunit structure

Monomer By similarity. HAMAP-Rule MF_02002

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02002.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02002

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processisoleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

isoleucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 988988Isoleucine--tRNA ligase HAMAP-Rule MF_02002
PRO_0000098492

Regions

Motif60 – 7011"HIGH" region HAMAP-Rule MF_02002
Motif611 – 6155"KMSKS" region HAMAP-Rule MF_02002

Sites

Metal binding9571Zinc By similarity
Metal binding9601Zinc By similarity
Metal binding9771Zinc By similarity
Metal binding9801Zinc By similarity
Binding site5701Aminoacyl-adenylate By similarity
Binding site6141ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P73505 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 13423806C0E4F0A0

FASTA988112,346
        10         20         30         40         50         60 
MTEAKSYKDT VNLPQTRFDM RANANKREPE IQQYWQEKGI YADLADNNPK DLFVLHDGPP 

        70         80         90        100        110        120 
YANGALHMGH ALNKVLKDII NKYKLLQGHK VHYVPGWDCH GLPIELKVLQ SLKSSERAEL 

       130        140        150        160        170        180 
TPLTLRHKAR DFALKAQQEQ AVGFQRYGIW GDWKKPYLTL TPEYEAAQIG VFGAMALKGY 

       190        200        210        220        230        240 
IYRGLKPVHW SPSSRTALAE AELEYPEGHT SRSIYVSFPI TQAGEKAAEI LAPYIDDLAV 

       250        260        270        280        290        300 
AIWTTTPWTL PGNLAVALNP ELTYAVVETE SHIFHRSYLI VALDLVEKLS ETFGVKLTVK 

       310        320        330        340        350        360 
VTLQGESLEN TCYQHPLFDR VSPIVIGGDY VTTESGTGLV HTAPGHGQED YVVGQRYGLP 

       370        380        390        400        410        420 
ILSPVDAAGN LTEEAGKFAG LNVLNDANEA IINALQDQKV LLKEEAYEHK YPYDWRTKKP 

       430        440        450        460        470        480 
TIFRATEQWF ASVEGFRDQA LKAIKEVTWI PTQGENRITP MVGDRSDWCI SRQRAWGVPI 

       490        500        510        520        530        540 
PVFYDEETSE PLLTEETINH VQQIIAEKGS DAWWELTVEE LLPEAYRNNG RTYRKGEDTM 

       550        560        570        580        590        600 
DVWFDSGSSW AAVANAKNRP LKYPVDMYLE GSDQHRGWFQ SSLLTSVAVN GIAPYKTVLT 

       610        620        630        640        650        660 
HGFVLDEKGH KMSKSLGNVV DPYQIINGGK NQKQEPAYGA DVLRLWVASV DYANDVPIGQ 

       670        680        690        700        710        720 
GILKQLVDIR NKIRNTARFL LGNLHDFNPE TDAVAYEDLP ELDRYMLHRM TEVFTEVTEA 

       730        740        750        760        770        780 
YESFQFFKFF QTVQNFCVVD LSNFYLDIAK DRLYISAPNA PRRRSCQTIL QLALENLTKA 

       790        800        810        820        830        840 
IAPVLCHLAE DIWQFLPYEK PTESVFQSGW VNLDAQWQQP QLAQKWVSLR QLRDGINQLL 

       850        860        870        880        890        900 
EQARRDKVIG SSLEAKLIIV DPRTAESGTD EKQGENFVHW FYGWLENFNE PTFDPNDRKI 

       910        920        930        940        950        960 
SLRNIFLTSQ VEFIKSAEIE PSFTYEETVV INLERHDNNI RTKPIKIIVT KADGHKCERC 

       970        980 
WNYSTQVGSF SDDPTICERC NEALQGNF 

« Hide

References

[1]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. expand/collapse author list , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000022 Genomic DNA. Translation: BAA17545.1.
PIRS77211.
RefSeqNP_440865.1. NC_000911.1.
YP_005650924.1. NC_017277.1.
YP_007450748.1. NC_020286.1.

3D structure databases

ProteinModelPortalP73505.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP73505. 1 interaction.
STRING1148.sll1362.

Proteomic databases

PaxDbP73505.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA17545; BAA17545; BAA17545.
GeneID12255008.
14616400.
954168.
KEGGsyn:sll1362.
syy:SYNGTS_0971.
syz:MYO_19780.
PATRIC23838974. VBISynSp132158_1052.

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHOG000246402.
KOK01870.
OMAVLGDWDN.
OrthoDBEOG644ZM1.
PhylomeDBP73505.
ProtClustDBPRK05743.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02002. Ile_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYI_SYNY3
AccessionPrimary (citable) accession number: P73505
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: April 16, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Synechocystis PCC 6803

Synechocystis (strain PCC 6803): entries and gene names

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries