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P73505

- SYI_SYNY3

UniProt

P73505 - SYI_SYNY3

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei570 – 5701Aminoacyl-adenylateUniRule annotation
Binding sitei614 – 6141ATPUniRule annotation
Metal bindingi957 – 9571ZincUniRule annotation
Metal bindingi960 – 9601ZincUniRule annotation
Metal bindingi977 – 9771ZincUniRule annotation
Metal bindingi980 – 9801ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:sll1362
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 988988Isoleucine--tRNA ligasePRO_0000098492Add
BLAST

Proteomic databases

PaxDbiP73505.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

IntActiP73505. 1 interaction.
STRINGi1148.sll1362.

Structurei

3D structure databases

ProteinModelPortaliP73505.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi60 – 7011"HIGH" regionAdd
BLAST
Motifi611 – 6155"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
InParanoidiP73505.
KOiK01870.
OMAiLGHRVHY.
OrthoDBiEOG644ZM1.
PhylomeDBiP73505.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P73505-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTEAKSYKDT VNLPQTRFDM RANANKREPE IQQYWQEKGI YADLADNNPK
60 70 80 90 100
DLFVLHDGPP YANGALHMGH ALNKVLKDII NKYKLLQGHK VHYVPGWDCH
110 120 130 140 150
GLPIELKVLQ SLKSSERAEL TPLTLRHKAR DFALKAQQEQ AVGFQRYGIW
160 170 180 190 200
GDWKKPYLTL TPEYEAAQIG VFGAMALKGY IYRGLKPVHW SPSSRTALAE
210 220 230 240 250
AELEYPEGHT SRSIYVSFPI TQAGEKAAEI LAPYIDDLAV AIWTTTPWTL
260 270 280 290 300
PGNLAVALNP ELTYAVVETE SHIFHRSYLI VALDLVEKLS ETFGVKLTVK
310 320 330 340 350
VTLQGESLEN TCYQHPLFDR VSPIVIGGDY VTTESGTGLV HTAPGHGQED
360 370 380 390 400
YVVGQRYGLP ILSPVDAAGN LTEEAGKFAG LNVLNDANEA IINALQDQKV
410 420 430 440 450
LLKEEAYEHK YPYDWRTKKP TIFRATEQWF ASVEGFRDQA LKAIKEVTWI
460 470 480 490 500
PTQGENRITP MVGDRSDWCI SRQRAWGVPI PVFYDEETSE PLLTEETINH
510 520 530 540 550
VQQIIAEKGS DAWWELTVEE LLPEAYRNNG RTYRKGEDTM DVWFDSGSSW
560 570 580 590 600
AAVANAKNRP LKYPVDMYLE GSDQHRGWFQ SSLLTSVAVN GIAPYKTVLT
610 620 630 640 650
HGFVLDEKGH KMSKSLGNVV DPYQIINGGK NQKQEPAYGA DVLRLWVASV
660 670 680 690 700
DYANDVPIGQ GILKQLVDIR NKIRNTARFL LGNLHDFNPE TDAVAYEDLP
710 720 730 740 750
ELDRYMLHRM TEVFTEVTEA YESFQFFKFF QTVQNFCVVD LSNFYLDIAK
760 770 780 790 800
DRLYISAPNA PRRRSCQTIL QLALENLTKA IAPVLCHLAE DIWQFLPYEK
810 820 830 840 850
PTESVFQSGW VNLDAQWQQP QLAQKWVSLR QLRDGINQLL EQARRDKVIG
860 870 880 890 900
SSLEAKLIIV DPRTAESGTD EKQGENFVHW FYGWLENFNE PTFDPNDRKI
910 920 930 940 950
SLRNIFLTSQ VEFIKSAEIE PSFTYEETVV INLERHDNNI RTKPIKIIVT
960 970 980
KADGHKCERC WNYSTQVGSF SDDPTICERC NEALQGNF
Length:988
Mass (Da):112,346
Last modified:February 1, 1997 - v1
Checksum:i13423806C0E4F0A0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000022 Genomic DNA. Translation: BAA17545.1.
PIRiS77211.
RefSeqiNP_440865.1. NC_000911.1.
YP_005650924.1. NC_017277.1.
YP_007450748.1. NC_020286.1.

Genome annotation databases

EnsemblBacteriaiBAA17545; BAA17545; BAA17545.
GeneIDi954168.
KEGGisyn:sll1362.
syy:SYNGTS_0971.
syz:MYO_19780.
PATRICi23838974. VBISynSp132158_1052.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000022 Genomic DNA. Translation: BAA17545.1 .
PIRi S77211.
RefSeqi NP_440865.1. NC_000911.1.
YP_005650924.1. NC_017277.1.
YP_007450748.1. NC_020286.1.

3D structure databases

ProteinModelPortali P73505.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P73505. 1 interaction.
STRINGi 1148.sll1362.

Proteomic databases

PaxDbi P73505.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAA17545 ; BAA17545 ; BAA17545 .
GeneIDi 954168.
KEGGi syn:sll1362.
syy:SYNGTS_0971.
syz:MYO_19780.
PATRICi 23838974. VBISynSp132158_1052.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
InParanoidi P73505.
KOi K01870.
OMAi LGHRVHY.
OrthoDBi EOG644ZM1.
PhylomeDBi P73505.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.

Entry informationi

Entry nameiSYI_SYNY3
AccessioniPrimary (citable) accession number: P73505
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: October 29, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3