P73505 (SYI_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isoleucine--tRNA ligase
EC=6.1.1.5

Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS

Gene names

Name:	ileS
Ordered Locus Names:	sll1362

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechocystis › Synechocystis sp. PCC 6803

Protein attributes

Sequence length	988 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02002
Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02002
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 988	988	Isoleucine--tRNA ligase HAMAP-Rule MF_02002		PRO_0000098492
Regions
	Motif	60 – 70	11	"HIGH" region HAMAP-Rule MF_02002
	Motif	611 – 615	5	"KMSKS" region HAMAP-Rule MF_02002
Sites
	Metal binding	957	1	Zinc By similarity
	Metal binding	960	1	Zinc By similarity
	Metal binding	977	1	Zinc By similarity
	Metal binding	980	1	Zinc By similarity
	Binding site	570	1	Aminoacyl-adenylate By similarity
	Binding site	614	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P73505 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 13423806C0E4F0A0	FASTA	988	112,346
	10 20 30 40 50 60 MTEAKSYKDT VNLPQTRFDM RANANKREPE IQQYWQEKGI YADLADNNPK DLFVLHDGPP 70 80 90 100 110 120 YANGALHMGH ALNKVLKDII NKYKLLQGHK VHYVPGWDCH GLPIELKVLQ SLKSSERAEL 130 140 150 160 170 180 TPLTLRHKAR DFALKAQQEQ AVGFQRYGIW GDWKKPYLTL TPEYEAAQIG VFGAMALKGY 190 200 210 220 230 240 IYRGLKPVHW SPSSRTALAE AELEYPEGHT SRSIYVSFPI TQAGEKAAEI LAPYIDDLAV 250 260 270 280 290 300 AIWTTTPWTL PGNLAVALNP ELTYAVVETE SHIFHRSYLI VALDLVEKLS ETFGVKLTVK 310 320 330 340 350 360 VTLQGESLEN TCYQHPLFDR VSPIVIGGDY VTTESGTGLV HTAPGHGQED YVVGQRYGLP 370 380 390 400 410 420 ILSPVDAAGN LTEEAGKFAG LNVLNDANEA IINALQDQKV LLKEEAYEHK YPYDWRTKKP 430 440 450 460 470 480 TIFRATEQWF ASVEGFRDQA LKAIKEVTWI PTQGENRITP MVGDRSDWCI SRQRAWGVPI 490 500 510 520 530 540 PVFYDEETSE PLLTEETINH VQQIIAEKGS DAWWELTVEE LLPEAYRNNG RTYRKGEDTM 550 560 570 580 590 600 DVWFDSGSSW AAVANAKNRP LKYPVDMYLE GSDQHRGWFQ SSLLTSVAVN GIAPYKTVLT 610 620 630 640 650 660 HGFVLDEKGH KMSKSLGNVV DPYQIINGGK NQKQEPAYGA DVLRLWVASV DYANDVPIGQ 670 680 690 700 710 720 GILKQLVDIR NKIRNTARFL LGNLHDFNPE TDAVAYEDLP ELDRYMLHRM TEVFTEVTEA 730 740 750 760 770 780 YESFQFFKFF QTVQNFCVVD LSNFYLDIAK DRLYISAPNA PRRRSCQTIL QLALENLTKA 790 800 810 820 830 840 IAPVLCHLAE DIWQFLPYEK PTESVFQSGW VNLDAQWQQP QLAQKWVSLR QLRDGINQLL 850 860 870 880 890 900 EQARRDKVIG SSLEAKLIIV DPRTAESGTD EKQGENFVHW FYGWLENFNE PTFDPNDRKI 910 920 930 940 950 960 SLRNIFLTSQ VEFIKSAEIE PSFTYEETVV INLERHDNNI RTKPIKIIVT KADGHKCERC 970 980 WNYSTQVGSF SDDPTICERC NEALQGNF

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA17545.1.
PIR	S77211.
RefSeq	NP_440865.1. NC_000911.1. YP_005650924.1. NC_017277.1. YP_007450748.1. NC_020286.1.
3D structure databases
ProteinModelPortal	P73505.
ModBase	Search...
Protein-protein interaction databases
IntAct	P73505. 1 interaction.
STRING	1148.sll1362.
Proteomic databases
PaxDb	P73505.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAA17545; BAA17545; BAA17545.
GeneID	12255008. 14616400. 954168.
KEGG	syn:sll1362. syy:SYNGTS_0971.
PATRIC	23838974. VBISynSp132158_1052.
Phylogenomic databases
eggNOG	COG0060.
HOGENOM	HOG000246402.
KO	K01870.
OMA	KQVLTHG.
ProtClustDB	PRK05743.
Family and domain databases
Gene3D	3.40.50.620. 2 hits. 3.90.740.10. 1 hit.
HAMAP	MF_02002. Ile_tRNA_synth_type1.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-ligase. IPR023585. Ile-tRNA-ligase_type1. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. IPR010663. Znf_DNA_glyclase/IsotRNA_synth. [Graphical view]
PANTHER	PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. PF06827. zf-FPG_IleRS. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYI_SYNY3

Accession

Primary (citable) accession number: P73505

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program