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Protein

Glutathione synthetase

Gene

gshB

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg2+ or Mn2+ ion per subunit.By similarity

Pathwayi: glutathione biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glutathione synthetase (gshB)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi288Magnesium or manganeseUniRule annotation1
Metal bindingi290Magnesium or manganeseUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi159 – 215ATPUniRule annotationAdd BLAST57

GO - Molecular functioni

Keywordsi

Molecular functionLigase
Biological processGlutathione biosynthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.2.3 382
UniPathwayiUPA00142; UER00210

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
Alternative name(s):
GSH synthetaseUniRule annotation
Short name:
GSH-SUniRule annotation
Short name:
GSHaseUniRule annotation
Glutathione synthaseUniRule annotation
Gene namesi
Name:gshBUniRule annotation
Ordered Locus Names:slr1238
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesMerismopediaceaeSynechocystis
Proteomesi
  • UP000001425 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001974901 – 320Glutathione synthetaseAdd BLAST320

Proteomic databases

PaxDbiP73493
PRIDEiP73493

Interactioni

Protein-protein interaction databases

IntActiP73493, 1 interactor
STRINGi1148.SYNGTS_0959

Structurei

3D structure databases

ProteinModelPortaliP73493
SMRiP73493
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini133 – 317ATP-graspUniRule annotationAdd BLAST185

Sequence similaritiesi

Belongs to the prokaryotic GSH synthase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000265022
InParanoidiP73493
KOiK01920
OMAiWMRKDPP
PhylomeDBiP73493

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
HAMAPiMF_00162 GSH_S, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR006284 Glut_synth_pro
IPR004218 GSHS_ATP-bd
IPR004215 GSHS_N
IPR016185 PreATP-grasp_dom_sf
PANTHERiPTHR21621:SF4 PTHR21621:SF4, 1 hit
PfamiView protein in Pfam
PF02955 GSH-S_ATP, 1 hit
PF02951 GSH-S_N, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01380 glut_syn, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit

Sequencei

Sequence statusi: Complete.

P73493-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLAFIIDPL EKLDPGHDST VAIMEAAQKL GHEVFVTSVG DLAVINGQAW
60 70 80 90 100
AKLAAVRLQP VILVDGHWQV SQPWSELSKS QWMALTECQA VFMRKDPPVT
110 120 130 140 150
VQYLYATFIL ELLAPTKTMV INSPQGLREA NEKMYTLQFA AVMPPTVVSL
160 170 180 190 200
DKGLIRQFLE EHGAAVLKPL GGKAGEGILF LDPGDRNFNS LVEISTQHGK
210 220 230 240 250
EPVMVQRFLP EAKEGDKRII LLDGDPIGAV NRIPSGAEFR GNMAVGGQVA
260 270 280 290 300
PTTITSREIE ICALLKPKLQ ADGLYFVGID VIGGYLTEVN VTSPTGIREI
310 320
DRLEGVRLGE KVICWLEKQF
Length:320
Mass (Da):35,069
Last modified:February 1, 1997 - v1
Checksum:i6C7BD439A23D185A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA Translation: BAA17533.1
PIRiS77430

Genome annotation databases

EnsemblBacteriaiBAA17533; BAA17533; BAA17533
KEGGisyn:slr1238

Similar proteinsi

Entry informationi

Entry nameiGSHB_SYNY3
AccessioniPrimary (citable) accession number: P73493
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 1, 1997
Last modified: May 23, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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