P73493 (GSHB_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione synthetase
EC=6.3.2.3

Alternative name(s):
GSH synthetase
Short name=GSH-S
Short name=GSHase
Glutathione synthase

Gene names

Name:	gshB
Ordered Locus Names:	slr1238

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechocystis › Synechocystis sp. PCC 6803

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP-Rule MF_00162
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP-Rule MF_00162
Sequence similarities	Belongs to the prokaryotic GSH synthase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytosol Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glutathione synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	Glutathione synthetase HAMAP-Rule MF_00162		PRO_0000197490
Regions
	Domain	133 – 317	185	ATP-grasp
	Nucleotide binding	159 – 215	57	ATP By similarity
Sites
	Metal binding	288	1	Magnesium or manganese By similarity
	Metal binding	290	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	P73493 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 6C7BD439A23D185A	FASTA	320	35,069
	10 20 30 40 50 60 MKLAFIIDPL EKLDPGHDST VAIMEAAQKL GHEVFVTSVG DLAVINGQAW AKLAAVRLQP 70 80 90 100 110 120 VILVDGHWQV SQPWSELSKS QWMALTECQA VFMRKDPPVT VQYLYATFIL ELLAPTKTMV 130 140 150 160 170 180 INSPQGLREA NEKMYTLQFA AVMPPTVVSL DKGLIRQFLE EHGAAVLKPL GGKAGEGILF 190 200 210 220 230 240 LDPGDRNFNS LVEISTQHGK EPVMVQRFLP EAKEGDKRII LLDGDPIGAV NRIPSGAEFR 250 260 270 280 290 300 GNMAVGGQVA PTTITSREIE ICALLKPKLQ ADGLYFVGID VIGGYLTEVN VTSPTGIREI 310 320 DRLEGVRLGE KVICWLEKQF

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA17533.1.
PIR	S77430.
RefSeq	NP_440853.1. NC_000911.1. YP_005650912.1. NC_017277.1. YP_007450736.1. NC_020286.1.
3D structure databases
ProteinModelPortal	P73493.
ModBase	Search...
Protein-protein interaction databases
IntAct	P73493. 1 interaction.
STRING	1148.slr1238.
Proteomic databases
PaxDb	P73493.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAA17533; BAA17533; BAA17533.
GeneID	12254916. 14616388. 954156.
KEGG	syn:slr1238. syy:SYNGTS_0959.
PATRIC	23838946. VBISynSp132158_1038.
Phylogenomic databases
eggNOG	COG0189.
HOGENOM	HOG000265022.
KO	K01920.
OMA	WMRKDPP.
ProtClustDB	PRK05246.
Enzyme and pathway databases
UniPathway	UPA00142; UER00210.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 1 hit. 3.40.50.20. 1 hit.
HAMAP	MF_00162. GSH-S.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR006284. Glut_synth_pro. IPR004218. GSHS_ATP-bd. IPR004215. GSHS_N. IPR016185. PreATP-grasp_dom. [Graphical view]
Pfam	PF02955. GSH-S_ATP. 1 hit. PF02951. GSH-S_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMs	TIGR01380. glut_syn. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSHB_SYNY3

Accession

Primary (citable) accession number: P73493

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program